Rw6G010350

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010523
Physical Location & Seq
Forward (+)
17883476 .. 17899456
15981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G010350.1

Sequence Viewer

Length: 1146 bp
ATGGGCGTTGTTCAAGCAAAGCCAGAGAGACGACGACCTGTTTTCGAGCGAAAGACATCCTTTGCTAAGAATCATAGGGTTGTTCCCTTCAATGCCGAACCAAGGCCAACTGACGATCTCAGCAAGCATTTTGAAGACTTGATGAGATCGTATCAGGCGGATTGCAAAGATGGCAAAGACTTGCAAGGCTTCGACGCCGAACTCAGCTCCAGCGCCAAAGAAGTCATCCGCAGTACCGGTCTTGATCGGTTTAAGGAATGCCTAAAGGTAATCTTGTCATGCAAAGAACAGATACTTGCGGCAGAGGGTGTGCAGGCGAGGATGACGAAAAACCAAAAATTGATGGAGTACTTCAACAATAGCTTGCAGACACTTCAACTCTGCAGATTGGAATTGCAAGACTTTATCAACTCGATCGTGTCGAAGAAGCTCAACGAGTCTCAGAAGATGTATCACCGTTCTGCGGAAGCAGTTGTTGCCAAGGTGAACTCTCTGCGCGACCACCATAGAGACATGCTTGACAAGTTGAAAGATCGAATTCAGAAGCTTGATAAGAAGCTGGAGCTTACTCGTACTTTAAAGAAGGTCGTAAATGTGGTGTTCATGGGTATGTTGTCTGGTCTGTTTGTTTGCACCATCATGGCGGCGGCTATGGCTGCTCCGCCAGTTGCGTCAGCCTTGACCTCTCATCCATGTATTGCCGGAGTTATTGCTGGTGGTGCCGTTGGTTATGCTTTTTCGTCAGTATTAAAAGAGGGACAACACTGGCTTCTGACTTTGATTCAAGGTTATGAATCAACTCTGGAGGCACATACGGGTGTAATTAAGTCGATGGAAGCCGGAACCAGAGACGCCATTAAAGAGTTGGATGACATCATGTGTCATGTTAATAAGGTCATTGCAAAAGGGAATGATCCAGTGTTGTCCAATTTAAGCTCTGCAAATGATGAGGGAAGAGTGGATGTGGTTGATCAGGAAGTGACGTCTATTCTGGAAAAGCTGGCAGAGTTTGGCAAGGAAATTGAGGAACTGGACGAGAAAAGAGAGCAGTGCCTCCGGGTTATAGAGACTGCAAGGGATAAGGTTCTAGGGGAATATGGCGAATTGAAGAGTAGCCCAGTGCGGAACACGAAGATCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

381

Amino Acids

42.64

Weight (kDa)

8.48

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 144 - 364 1.4e-24 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 986
AccB1I GGYRCC 1 cut(s) 719
AccII CGCG 1 cut(s) 498
AciI CCGC 8 cut(s) 158, 229, 299, 464, 644, 647, 662, 1123
AclWI GGATC 1 cut(s) 908
AcsI RAATTY 1 cut(s) 537
AcyI GRCGYC 3 cut(s) 195, 852, 983
AfaI GTAC 3 cut(s) 235, 350, 574
AfiI CCNNNNNNNGG 2 cut(s) 102, 463
AgeI ACCGGT 1 cut(s) 236
AgsI TTSAA 8 cut(s) 14, 91, 134, 355, 377, 529, 785, 1108
AluBI AGCT 8 cut(s) 207, 363, 430, 547, 559, 565, 936, 1000
AluI AGCT 8 cut(s) 207, 363, 430, 547, 559, 565, 936, 1000
Alw26I GTCTC 5 cut(s) 22, 444, 504, 843, 1061
AlwI GGATC 1 cut(s) 908
AoxI GGCC 1 cut(s) 104
ApeKI GCWGC 1 cut(s) 656
ApoI RAATTY 1 cut(s) 537
AsiGI ACCGGT 1 cut(s) 236
AspLEI GCGC 2 cut(s) 215, 498
AsuC2I CCSGG 1 cut(s) 1058
AsuHPI GGTGA 2 cut(s) 446, 496
BanI GGYRCC 1 cut(s) 719
BbsI GAAGAC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 643
BccI CCATC 4 cut(s) 164, 337, 644, 826
BceAI ACGGC 1 cut(s) 707
BclI TGATCA 1 cut(s) 970
BcnI CCSGG 1 cut(s) 1058
BcoDI GTCTC 5 cut(s) 22, 444, 504, 843, 1061
BfaI CTAG 1 cut(s) 1088
BfmI CTRYAG 1 cut(s) 382
BfoI RGCGCY 1 cut(s) 216
BisI GCNGC 4 cut(s) 300, 645, 648, 657
BlsI GCNGC 4 cut(s) 301, 646, 649, 658
BmcAI AGTACT 1 cut(s) 350
Bme1390I CCNGG 1 cut(s) 1058
BmiI GGNNCC 2 cut(s) 721, 844
BmrFI CCNGG 1 cut(s) 1058
BmrI ACTGGG 1 cut(s) 1112
BmuI ACTGGG 1 cut(s) 1112
BpiI GAAGAC 1 cut(s) 141
BpmI CTGGAG 3 cut(s) 193, 581, 824
BpuMI CCSGG 1 cut(s) 1058
BsaHI GRCGYC 3 cut(s) 195, 852, 983
BsaJI CCNNGG 2 cut(s) 101, 480
BsaWI WCCGGW 1 cut(s) 236
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 463
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 5 cut(s) 665, 770, 917, 1035, 1118
Bse3DI GCAATG 1 cut(s) 897
BseDI CCNNGG 2 cut(s) 101, 480
BseGI GGATG 6 cut(s) 56, 225, 327, 688, 874, 967
BseLI CCNNNNNNNGG 2 cut(s) 102, 463
BseMI GCAATG 1 cut(s) 897
BseMII CTCAG 3 cut(s) 133, 217, 455
BseNI ACTGG 5 cut(s) 665, 770, 917, 1035, 1118
BseXI GCAGC 1 cut(s) 643
BsgI GTGCAG 1 cut(s) 332
Bsh1236I CGCG 1 cut(s) 498
Bsh1285I CGRYCG 1 cut(s) 417
BshFI GGCC 1 cut(s) 106
BshNI GGYRCC 1 cut(s) 719
BshTI ACCGGT 1 cut(s) 236
BsiEI CGRYCG 1 cut(s) 417
BsiSI CCGG 4 cut(s) 237, 702, 840, 1057
BslFI GGGAC 1 cut(s) 771
BslI CCNNNNNNNGG 2 cut(s) 102, 463
BsmAI GTCTC 5 cut(s) 22, 444, 504, 843, 1061
BsmBI CGTCTC 2 cut(s) 22, 843
BsmFI GGGAC 1 cut(s) 771
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 1 cut(s) 106
Bsp143I GATC 8 cut(s) 115, 146, 244, 414, 532, 913, 970, 1134
BspACI CCGC 8 cut(s) 158, 229, 299, 464, 644, 647, 662, 1123
BspANI GGCC 1 cut(s) 106
BspCNI CTCAG 3 cut(s) 132, 216, 454
BspFNI CGCG 1 cut(s) 498
BspLI GGNNCC 2 cut(s) 721, 844
BspMAI CTGCAG 1 cut(s) 386
BspPI GGATC 1 cut(s) 908
BspT107I GGYRCC 1 cut(s) 719
BsrDI GCAATG 1 cut(s) 897
BsrFI RCCGGY 1 cut(s) 236
BsrI ACTGG 5 cut(s) 665, 770, 917, 1035, 1118
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 2 cut(s) 101, 480
BssMI GATC 8 cut(s) 115, 146, 244, 414, 532, 913, 970, 1134
BssNI GRCGYC 3 cut(s) 195, 852, 983
BssT1I CCWWGG 2 cut(s) 101, 480
Bst4CI ACNGT 1 cut(s) 458
Bst6I CTCTTC 2 cut(s) 949, 1103
BstACI GRCGYC 3 cut(s) 195, 852, 983
BstAPI GCANNNNNTGC 1 cut(s) 476
BstC8I GCNNGC 4 cut(s) 125, 315, 365, 1002
BstDEI CTNAG 4 cut(s) 66, 119, 203, 441
BstF5I GGATG 6 cut(s) 56, 225, 327, 688, 874, 967
BstFNI CGCG 1 cut(s) 498
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 2 cut(s) 215, 498
BstKTI GATC 8 cut(s) 118, 149, 247, 417, 535, 916, 973, 1137
BstMAI GTCTC 5 cut(s) 22, 444, 504, 843, 1061
BstMBI GATC 8 cut(s) 115, 146, 244, 414, 532, 913, 970, 1134
BstMCI CGRYCG 1 cut(s) 417
BstMWI GCNNNNNNNGC 5 cut(s) 171, 476, 653, 656, 719
BstNSI RCATGY 1 cut(s) 517
BstSCI CCNGG 1 cut(s) 1056
BstSFI CTRYAG 1 cut(s) 382
BstUI CGCG 1 cut(s) 498
BstV1I GCAGC 1 cut(s) 643
BstV2I GAAGAC 1 cut(s) 141
BsuRI GGCC 1 cut(s) 106
BtsCI GGATG 6 cut(s) 56, 225, 327, 688, 874, 967
BtsI GCAGTG 1 cut(s) 1055
BtsIMutI CAGTG 4 cut(s) 763, 924, 1055, 1125
Cac8I GCNNGC 4 cut(s) 125, 315, 365, 1002
CfoI GCGC 2 cut(s) 215, 498
Cfr10I RCCGGY 1 cut(s) 236
CseI GACGC 3 cut(s) 203, 660, 860
Csp6I GTAC 3 cut(s) 234, 349, 573
CspAI ACCGGT 1 cut(s) 236
CviAII CATG 7 cut(s) 279, 514, 604, 640, 693, 877, 884
CviQI GTAC 3 cut(s) 234, 349, 573
DdeI CTNAG 4 cut(s) 66, 119, 203, 441
DpnI GATC 8 cut(s) 117, 148, 246, 416, 534, 915, 972, 1136
DpnII GATC 8 cut(s) 115, 146, 244, 414, 532, 913, 970, 1134
DraI TTTAAA 1 cut(s) 579
Eam1104I CTCTTC 2 cut(s) 949, 1103
EarI CTCTTC 2 cut(s) 949, 1103
EciI GGCGGA 2 cut(s) 173, 651
Eco130I CCWWGG 2 cut(s) 101, 480
EcoRI GAATTC 1 cut(s) 537
EcoT14I CCWWGG 2 cut(s) 101, 480
ErhI CCWWGG 2 cut(s) 101, 480
Esp3I CGTCTC 2 cut(s) 22, 843
FaeI CATG 7 cut(s) 282, 517, 607, 643, 696, 880, 887
FalI AAGNNNNNCTT 4 cut(s) 44, 76, 257, 289
FaqI GGGAC 1 cut(s) 771
FatI CATG 7 cut(s) 278, 513, 603, 639, 692, 876, 883
FbaI TGATCA 1 cut(s) 970
Fnu4HI GCNGC 4 cut(s) 300, 645, 648, 657
FokI GGATG 6 cut(s) 43, 212, 334, 675, 881, 974
Fsp4HI GCNGC 4 cut(s) 300, 645, 648, 657
FspBI CTAG 1 cut(s) 1088
GlaI GCGC 2 cut(s) 214, 497
GluI GCNGC 4 cut(s) 300, 645, 648, 657
GsuI CTGGAG 3 cut(s) 193, 581, 824
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 1 cut(s) 106
HapII CCGG 4 cut(s) 237, 702, 840, 1057
HgaI GACGC 3 cut(s) 203, 660, 860
HhaI GCGC 2 cut(s) 215, 498
Hin1I GRCGYC 3 cut(s) 195, 852, 983
Hin1II CATG 7 cut(s) 282, 517, 607, 643, 696, 880, 887
Hin6I GCGC 2 cut(s) 213, 496
HinP1I GCGC 2 cut(s) 213, 496
HindIII AAGCTT 1 cut(s) 545
HinfI GANTC 4 cut(s) 70, 437, 781, 794
HpaII CCGG 4 cut(s) 237, 702, 840, 1057
HphI GGTGA 2 cut(s) 446, 496
Hpy166II GTNNAC 1 cut(s) 487
Hpy188I TCNGA 3 cut(s) 444, 543, 774
Hpy188III TCNNGA 5 cut(s) 242, 803, 974, 992, 1138
Hpy8I GTNNAC 1 cut(s) 487
Hpy99I CGWCG 2 cut(s) 36, 197
HpyAV CCTTC 2 cut(s) 97, 577
HpyCH4III ACNGT 1 cut(s) 458
HpyCH4IV ACGT 1 cut(s) 983
HpyF10VI GCNNNNNNNGC 5 cut(s) 171, 476, 653, 656, 719
HpyF3I CTNAG 4 cut(s) 66, 119, 203, 441
HpySE526I ACGT 1 cut(s) 983
Hsp92I GRCGYC 3 cut(s) 195, 852, 983
Hsp92II CATG 7 cut(s) 282, 517, 607, 643, 696, 880, 887
HspAI GCGC 2 cut(s) 213, 496
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 8 cut(s) 115, 146, 244, 414, 532, 913, 970, 1134
LmnI GCTCC 3 cut(s) 212, 562, 664
Lsp1109I GCAGC 1 cut(s) 643
MaeI CTAG 1 cut(s) 1088
MaeII ACGT 1 cut(s) 983
MaeIII GTNAC 1 cut(s) 979
MalI GATC 8 cut(s) 117, 148, 246, 416, 534, 915, 972, 1136
MboI GATC 8 cut(s) 115, 146, 244, 414, 532, 913, 970, 1134
MboII GAAGA 6 cut(s) 146, 436, 457, 966, 1120, 1144
MluCI AATT 7 cut(s) 338, 392, 537, 822, 928, 1020, 1103
MlyI GAGTC 1 cut(s) 446
MmeI TCCRAC 1 cut(s) 846
MnlI CCTC 8 cut(s) 298, 312, 694, 748, 799, 943, 1018, 1064
MseI TTAA 7 cut(s) 252, 578, 749, 825, 858, 888, 932
MslI CAYNNNNRTG 3 cut(s) 608, 638, 816
MspI CCGG 4 cut(s) 237, 702, 840, 1057
MspR9I CCNGG 1 cut(s) 1058
Mva1269I GAATGC 1 cut(s) 263
MvnI CGCG 1 cut(s) 498
MwoI GCNNNNNNNGC 5 cut(s) 171, 476, 653, 656, 719
NciI CCSGG 1 cut(s) 1058
NdeII GATC 8 cut(s) 115, 146, 244, 414, 532, 913, 970, 1134
NlaIII CATG 7 cut(s) 282, 517, 607, 643, 696, 880, 887
NlaIV GGNNCC 2 cut(s) 721, 844
NmuCI GTSAC 1 cut(s) 979
NspI RCATGY 1 cut(s) 517
PcsI WCGNNNNNNNCGW 1 cut(s) 419
PctI GAATGC 1 cut(s) 263
PfeI GAWTC 3 cut(s) 70, 781, 794
PinAI ACCGGT 1 cut(s) 236
PkrI GCNGC 4 cut(s) 301, 646, 649, 658
Ple19I CGATCG 1 cut(s) 417
PleI GAGTC 1 cut(s) 445
PpsI GAGTC 1 cut(s) 445
PspN4I GGNNCC 2 cut(s) 721, 844
PstI CTGCAG 1 cut(s) 386
PvuI CGATCG 1 cut(s) 417
RsaI GTAC 3 cut(s) 235, 350, 574
RsaNI GTAC 3 cut(s) 234, 349, 573
RseI CAYNNNNRTG 3 cut(s) 608, 638, 816
SaqAI TTAA 7 cut(s) 252, 578, 749, 825, 858, 888, 932
SatI GCNGC 4 cut(s) 300, 645, 648, 657
Sau3AI GATC 8 cut(s) 115, 146, 244, 414, 532, 913, 970, 1134
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 1 cut(s) 446
ScrFI CCNGG 1 cut(s) 1058
SfcI CTRYAG 1 cut(s) 382
SmiMI CAYNNNNRTG 3 cut(s) 608, 638, 816
Sse9I AATT 7 cut(s) 338, 392, 537, 822, 928, 1020, 1103
SsiI CCGC 8 cut(s) 158, 229, 299, 464, 644, 647, 662, 1123
SspMI CTAG 1 cut(s) 1088
StyD4I CCNGG 1 cut(s) 1056
StyI CCWWGG 2 cut(s) 101, 480
TaaI ACNGT 1 cut(s) 458
TaiI ACGT 1 cut(s) 986
TaqI TCGA 6 cut(s) 45, 192, 413, 422, 535, 830
TasI AATT 7 cut(s) 338, 392, 537, 822, 928, 1020, 1103
TatI WGTACW 1 cut(s) 348
TauI GCSGC 3 cut(s) 302, 647, 650
TfiI GAWTC 3 cut(s) 70, 781, 794
Tru1I TTAA 7 cut(s) 252, 578, 749, 825, 858, 888, 932
Tru9I TTAA 7 cut(s) 252, 578, 749, 825, 858, 888, 932
TscAI CASTG 4 cut(s) 770, 924, 1055, 1125
TseFI GTSAC 1 cut(s) 979
TseI GCWGC 1 cut(s) 656
Tsp45I GTSAC 1 cut(s) 979
TspDTI ATGAA 2 cut(s) 592, 807
TspRI CASTG 4 cut(s) 770, 924, 1055, 1125
XapI RAATTY 1 cut(s) 537
XceI RCATGY 1 cut(s) 517
XcmI CCANNNNNNNNNTGG 1 cut(s) 862
XspI CTAG 1 cut(s) 1088
ZraI GACGTC 1 cut(s) 984
ZrmI AGTACT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.