Rh6DG102100

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0010523
Physical Location & Seq
Forward (+)
12854854 .. 12857228
2375 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG102100.1

Sequence Viewer

Length: 1197 bp
ATGGGCGGTGCTCAAGCAAAGGCTGCAAAGCGAGAGAGAGGACGAGCAGGTTTGGAGCGAAAGACATCCGCTAAGAATCATAGGGTTGTTCCCGAACCAAGGCCAACTGATGACCTCTGCAAGGATTTTGAAGATGGCAAAGACTTGCAAGACTTCGCCGCCGCACTCGGCTCCAGCGCCAAAGAAGTCATCTGCAGTACCGGTTTTGATCGGTTTGAGAAATACCTAATGTATCTGAAGGATTCCGACGAGAAGACGAAGGAGGTAATCTTGTCATGCATAAAACAGATACTTGAGGCAGAGGATGTGCAGGCGATGACGGAGGATGAGAAGTTCTTAGATGATTTGATCAAAATGTACACCAACAATAGCGGGCAGACGCATCAACTCTGCCGAGGGCAATTGCGAGAATTCATCAAATCGGCTCGTGCGGTCCATTCATGCATTACAGATGTGCTTGAGAAGGGAGTGTCGAAGAAGCTCAACGAATCTGTGACGAAGTATCGCCTTGATGCGGAAGAAGTAGTTGCAAAAGTGAAATCTCTGCGCGACCAACACATAGACATGCTTCACGAGTTGGAAGATCGAATTCAGAAGTGTAATAAGAAGCTGGAGCGTATTCGTACTTTAAAGAAGGTTGTACATGTGGTGTTCATAGGTATGTTGTCTGGTCTGTTTGTTTGCACCATCATGGCGGCGGCTATGGCTGCTCCTCCAGTTGCGTCAGCCTTGACGTCTCATCCATGTATTGCCGGAGTTGTTGCTGGTGGTGCCGTTGGTTATGCTTGTTCGTCAGTATTAAAAGAGGGACGACACTGGCTTCTCACTGTGATTCAAGATTGTAAATCAACTCTGGAGGCACGTAAGGGTGTAATTCAGTCGATGGAAGCCGGAACCGGAGACACCATTAAAGAGTTGGATGCCATCATGTGTCTTGTTAAGAAGGTCATTGCAAAGGGGAATGATCCAGTGTTGTCCAATTTAAGCTCTGCAAATGATGAGGGAAGAGTAGATGTGGAAATTGATGAGAAAGAGACTATTGAAAAGTGGCGGACAGAGTTTCACAAAGCAATTGAGGAATTGGACAAGAAAAGGGAGGACTGCCTCGGGGTTGTAGAGAAGGCAAGGAAAAAGGTTCAAGGGGAATATGACGAATTGAAGAGGAGCCCAGTGTTGAACACGAAGATCAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

398

Amino Acids

44.32

Weight (kDa)

8.14

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 64 - 379 1.8e-21 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 737
Acc36I ACCTGC 1 cut(s) 38
AccB1I GGYRCC 1 cut(s) 770
AccII CGCG 1 cut(s) 549
AclWI GGATC 1 cut(s) 959
AcsI RAATTY 2 cut(s) 410, 588
AcuI CTGAAG 1 cut(s) 257
AcyI GRCGYC 1 cut(s) 734
AfaI GTAC 4 cut(s) 199, 359, 625, 642
AfiI CCNNNNNNNGG 3 cut(s) 99, 121, 514
AflIII ACRYGT 1 cut(s) 643
AgeI ACCGGT 1 cut(s) 200
AgsI TTSAA 6 cut(s) 131, 836, 1043, 1139, 1159, 1177
AloI GAACNNNNNNTCC 2 cut(s) 317, 349
AluBI AGCT 3 cut(s) 481, 610, 987
AluI AGCT 3 cut(s) 481, 610, 987
Alw21I GWGCWC 1 cut(s) 13
Alw26I GTCTC 3 cut(s) 741, 894, 1028
AlwI GGATC 1 cut(s) 959
Ama87I CYCGRG 1 cut(s) 1106
AoxI GGCC 1 cut(s) 101
ApeKI GCWGC 2 cut(s) 23, 707
ApoI RAATTY 2 cut(s) 410, 588
AsiGI ACCGGT 1 cut(s) 200
AspLEI GCGC 2 cut(s) 179, 549
AspS9I GGNCC 1 cut(s) 433
AvaI CYCGRG 1 cut(s) 1106
AvaII GGWCC 1 cut(s) 433
BanI GGYRCC 1 cut(s) 770
BanII GRGCYC 1 cut(s) 1169
BauI CACGAG 2 cut(s) 426, 572
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 13
BbvI GCAGC 2 cut(s) 10, 694
BccI CCATC 4 cut(s) 128, 695, 877, 932
BceAI ACGGC 1 cut(s) 758
BclI TGATCA 1 cut(s) 348
BcoDI GTCTC 3 cut(s) 741, 894, 1028
BfmI CTRYAG 1 cut(s) 193
BfoI RGCGCY 1 cut(s) 180
BfuAI ACCTGC 1 cut(s) 38
BisI GCNGC 6 cut(s) 24, 159, 162, 696, 699, 708
BlsI GCNGC 6 cut(s) 25, 160, 163, 697, 700, 709
Bme18I GGWCC 1 cut(s) 433
BmeT110I CYCGRG 1 cut(s) 1106
BmgT120I GGNCC 1 cut(s) 433
BmiI GGNNCC 4 cut(s) 172, 772, 895, 1166
BmrI ACTGGG 1 cut(s) 1163
BmsI GCATC 3 cut(s) 391, 502, 910
BmuI ACTGGG 1 cut(s) 1163
BpiI GAAGAC 1 cut(s) 260
BpmI CTGGAG 4 cut(s) 157, 632, 699, 875
BpuEI CTTGAG 2 cut(s) 314, 479
BsaAI YACGTR 1 cut(s) 863
BsaHI GRCGYC 1 cut(s) 734
BsaJI CCNNGG 3 cut(s) 98, 394, 1105
BsaWI WCCGGW 2 cut(s) 200, 896
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 121, 514
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 4 cut(s) 716, 821, 968, 1169
Bse3DI GCAATG 1 cut(s) 948
BseDI CCNNGG 3 cut(s) 98, 394, 1105
BseGI GGATG 5 cut(s) 65, 310, 331, 739, 925
BseLI CCNNNNNNNGG 3 cut(s) 99, 121, 514
BseMI GCAATG 1 cut(s) 948
BseNI ACTGG 4 cut(s) 716, 821, 968, 1169
BseRI GAGGAG 2 cut(s) 702, 1177
BseXI GCAGC 2 cut(s) 10, 694
BsgI GTGCAG 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 549
BshFI GGCC 1 cut(s) 103
BshNI GGYRCC 1 cut(s) 770
BshTI ACCGGT 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 13
BsiHKCI CYCGRG 1 cut(s) 1106
BsiSI CCGG 4 cut(s) 201, 753, 891, 897
BslFI GGGAC 1 cut(s) 822
BslI CCNNNNNNNGG 3 cut(s) 99, 121, 514
BsmAI GTCTC 3 cut(s) 741, 894, 1028
BsmBI CGTCTC 1 cut(s) 741
BsmFI GGGAC 1 cut(s) 822
BsnI GGCC 1 cut(s) 103
BsoBI CYCGRG 1 cut(s) 1106
Bsp1286I GDGCHC 2 cut(s) 13, 1169
Bsp1407I TGTACA 2 cut(s) 357, 640
Bsp143I GATC 5 cut(s) 208, 348, 583, 964, 1185
BspANI GGCC 1 cut(s) 103
BspFNI CGCG 1 cut(s) 549
BspLI GGNNCC 4 cut(s) 172, 772, 895, 1166
BspMAI CTGCAG 1 cut(s) 197
BspMI ACCTGC 1 cut(s) 38
BspPI GGATC 1 cut(s) 959
BspT107I GGYRCC 1 cut(s) 770
BsrDI GCAATG 1 cut(s) 948
BsrFI RCCGGY 1 cut(s) 200
BsrGI TGTACA 2 cut(s) 357, 640
BsrI ACTGG 4 cut(s) 716, 821, 968, 1169
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 3 cut(s) 98, 394, 1105
BssMI GATC 5 cut(s) 208, 348, 583, 964, 1185
BssNI GRCGYC 1 cut(s) 734
BssSI CACGAG 2 cut(s) 426, 572
BssT1I CCWWGG 1 cut(s) 98
Bst2BI CACGAG 2 cut(s) 426, 572
Bst4CI ACNGT 1 cut(s) 829
Bst6I CTCTTC 2 cut(s) 1000, 1154
BstACI GRCGYC 1 cut(s) 734
BstAPI GCANNNNNTGC 1 cut(s) 23
BstAUI TGTACA 2 cut(s) 357, 640
BstBAI YACGTR 1 cut(s) 863
BstC8I GCNNGC 2 cut(s) 312, 374
BstDEI CTNAG 2 cut(s) 72, 337
BstENI CCTNNNNNAGG 1 cut(s) 119
BstF5I GGATG 5 cut(s) 65, 310, 331, 739, 925
BstFNI CGCG 1 cut(s) 549
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 2 cut(s) 179, 549
BstKTI GATC 5 cut(s) 211, 351, 586, 967, 1188
BstMAI GTCTC 3 cut(s) 741, 894, 1028
BstMBI GATC 5 cut(s) 208, 348, 583, 964, 1185
BstMWI GCNNNNNNNGC 4 cut(s) 23, 704, 707, 770
BstNSI RCATGY 2 cut(s) 568, 647
BstSFI CTRYAG 1 cut(s) 193
BstUI CGCG 1 cut(s) 549
BstV1I GCAGC 2 cut(s) 10, 694
BstV2I GAAGAC 1 cut(s) 260
BsuRI GGCC 1 cut(s) 103
BtgZI GCGATG 1 cut(s) 329
BtsCI GGATG 5 cut(s) 65, 310, 331, 739, 925
BtsIMutI CAGTG 4 cut(s) 814, 825, 975, 1176
BveI ACCTGC 1 cut(s) 38
Cac8I GCNNGC 2 cut(s) 312, 374
CfoI GCGC 2 cut(s) 179, 549
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 1 cut(s) 433
CseI GACGC 2 cut(s) 388, 711
Csp6I GTAC 4 cut(s) 198, 358, 624, 641
CspAI ACCGGT 1 cut(s) 200
CviAII CATG 7 cut(s) 276, 441, 565, 644, 691, 744, 928
CviQI GTAC 4 cut(s) 198, 358, 624, 641
DdeI CTNAG 2 cut(s) 72, 337
DpnI GATC 5 cut(s) 210, 350, 585, 966, 1187
DpnII GATC 5 cut(s) 208, 348, 583, 964, 1185
DraI TTTAAA 1 cut(s) 630
Eam1104I CTCTTC 2 cut(s) 1000, 1154
EarI CTCTTC 2 cut(s) 1000, 1154
EciI GGCGGA 1 cut(s) 1066
Eco130I CCWWGG 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 1169
Eco47I GGWCC 1 cut(s) 433
Eco57I CTGAAG 1 cut(s) 257
Eco88I CYCGRG 1 cut(s) 1106
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoRI GAATTC 2 cut(s) 410, 588
EcoT14I CCWWGG 1 cut(s) 98
EcoT22I ATGCAT 2 cut(s) 281, 446
EcoT38I GRGCYC 1 cut(s) 1169
ErhI CCWWGG 1 cut(s) 98
Esp3I CGTCTC 1 cut(s) 741
FaeI CATG 7 cut(s) 279, 444, 568, 647, 694, 747, 931
FaqI GGGAC 1 cut(s) 822
FatI CATG 7 cut(s) 275, 440, 564, 643, 690, 743, 927
FauI CCCGC 1 cut(s) 365
FbaI TGATCA 1 cut(s) 348
Fnu4HI GCNGC 6 cut(s) 24, 159, 162, 696, 699, 708
FokI GGATG 5 cut(s) 52, 317, 338, 726, 932
FriOI GRGCYC 1 cut(s) 1169
Fsp4HI GCNGC 6 cut(s) 24, 159, 162, 696, 699, 708
GlaI GCGC 2 cut(s) 178, 548
GluI GCNGC 6 cut(s) 24, 159, 162, 696, 699, 708
GsuI CTGGAG 4 cut(s) 157, 632, 699, 875
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 1 cut(s) 103
HapII CCGG 4 cut(s) 201, 753, 891, 897
HgaI GACGC 2 cut(s) 388, 711
HhaI GCGC 2 cut(s) 179, 549
Hin1I GRCGYC 1 cut(s) 734
Hin1II CATG 7 cut(s) 279, 444, 568, 647, 694, 747, 931
Hin6I GCGC 2 cut(s) 177, 547
HinP1I GCGC 2 cut(s) 177, 547
HinfI GANTC 4 cut(s) 76, 242, 488, 832
HpaII CCGG 4 cut(s) 201, 753, 891, 897
Hpy166II GTNNAC 1 cut(s) 360
Hpy188I TCNGA 3 cut(s) 237, 247, 594
Hpy188III TCNNGA 5 cut(s) 92, 572, 836, 854, 1189
Hpy8I GTNNAC 1 cut(s) 360
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 6 cut(s) 232, 253, 457, 628, 937, 1114
HpyCH4III ACNGT 1 cut(s) 829
HpyCH4IV ACGT 2 cut(s) 734, 862
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 704, 707, 770
HpyF3I CTNAG 2 cut(s) 72, 337
HpySE526I ACGT 2 cut(s) 734, 862
Hsp92I GRCGYC 1 cut(s) 734
Hsp92II CATG 7 cut(s) 279, 444, 568, 647, 694, 747, 931
HspAI GCGC 2 cut(s) 177, 547
Ksp22I TGATCA 1 cut(s) 348
Kzo9I GATC 5 cut(s) 208, 348, 583, 964, 1185
LmnI GCTCC 5 cut(s) 55, 176, 613, 715, 1164
Lsp1109I GCAGC 2 cut(s) 10, 694
LweI GCATC 3 cut(s) 391, 502, 910
MaeII ACGT 2 cut(s) 734, 862
MaeIII GTNAC 1 cut(s) 493
MalI GATC 5 cut(s) 210, 350, 585, 966, 1187
MboI GATC 5 cut(s) 208, 348, 583, 964, 1185
MboII GAAGA 8 cut(s) 143, 265, 487, 530, 593, 1017, 1171, 1195
MfeI CAATTG 2 cut(s) 401, 1071
MhlI GDGCHC 2 cut(s) 13, 1169
MluCI AATT 9 cut(s) 401, 410, 588, 873, 979, 1020, 1071, 1079, 1154
MmeI TCCRAC 3 cut(s) 270, 558, 897
Mph1103I ATGCAT 2 cut(s) 281, 446
MseI TTAA 5 cut(s) 629, 800, 909, 939, 983
MslI CAYNNNNRTG 3 cut(s) 563, 659, 689
MspI CCGG 4 cut(s) 201, 753, 891, 897
MunI CAATTG 2 cut(s) 401, 1071
MvnI CGCG 1 cut(s) 549
MwoI GCNNNNNNNGC 4 cut(s) 23, 704, 707, 770
NdeII GATC 5 cut(s) 208, 348, 583, 964, 1185
NlaIII CATG 7 cut(s) 279, 444, 568, 647, 694, 747, 931
NlaIV GGNNCC 4 cut(s) 172, 772, 895, 1166
NmeAIII GCCGAG 2 cut(s) 147, 419
NmuCI GTSAC 1 cut(s) 493
NsiI ATGCAT 2 cut(s) 281, 446
NspI RCATGY 2 cut(s) 568, 647
PciI ACATGT 1 cut(s) 643
PfeI GAWTC 4 cut(s) 76, 242, 488, 832
PinAI ACCGGT 1 cut(s) 200
PkrI GCNGC 6 cut(s) 25, 160, 163, 697, 700, 709
Ppu21I YACGTR 1 cut(s) 863
PscI ACATGT 1 cut(s) 643
PspN4I GGNNCC 4 cut(s) 172, 772, 895, 1166
PspPI GGNCC 1 cut(s) 433
PstI CTGCAG 1 cut(s) 197
RsaI GTAC 4 cut(s) 199, 359, 625, 642
RsaNI GTAC 4 cut(s) 198, 358, 624, 641
RseI CAYNNNNRTG 3 cut(s) 563, 659, 689
SaqAI TTAA 5 cut(s) 629, 800, 909, 939, 983
SatI GCNGC 6 cut(s) 24, 159, 162, 696, 699, 708
Sau3AI GATC 5 cut(s) 208, 348, 583, 964, 1185
Sau96I GGNCC 1 cut(s) 433
SduI GDGCHC 2 cut(s) 13, 1169
SfaNI GCATC 3 cut(s) 391, 502, 910
SfcI CTRYAG 1 cut(s) 193
SinI GGWCC 1 cut(s) 433
SmiMI CAYNNNNRTG 3 cut(s) 563, 659, 689
SmlI CTYRAG 3 cut(s) 12, 293, 458
SmoI CTYRAG 3 cut(s) 12, 293, 458
Sse9I AATT 9 cut(s) 401, 410, 588, 873, 979, 1020, 1071, 1079, 1154
StyI CCWWGG 1 cut(s) 98
TaaI ACNGT 1 cut(s) 829
TaiI ACGT 2 cut(s) 737, 865
TaqI TCGA 3 cut(s) 473, 586, 881
TasI AATT 9 cut(s) 401, 410, 588, 873, 979, 1020, 1071, 1079, 1154
TatI WGTACW 2 cut(s) 357, 640
TauI GCSGC 4 cut(s) 161, 164, 698, 701
TfiI GAWTC 4 cut(s) 76, 242, 488, 832
Tru1I TTAA 5 cut(s) 629, 800, 909, 939, 983
Tru9I TTAA 5 cut(s) 629, 800, 909, 939, 983
TscAI CASTG 4 cut(s) 821, 832, 975, 1176
TseFI GTSAC 1 cut(s) 493
TseI GCWGC 2 cut(s) 23, 707
Tsp45I GTSAC 1 cut(s) 493
TspDTI ATGAA 3 cut(s) 403, 429, 643
TspGWI ACGGA 1 cut(s) 335
TspRI CASTG 4 cut(s) 821, 832, 975, 1176
VpaK11BI GGWCC 1 cut(s) 433
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 2 cut(s) 410, 588
XceI RCATGY 2 cut(s) 568, 647
XcmI CCANNNNNNNNNTGG 1 cut(s) 913
ZraI GACGTC 1 cut(s) 735
Zsp2I ATGCAT 2 cut(s) 281, 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.