Rorug06G0001100

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010523
Physical Location & Seq
Reverse (-)
237049 .. 247559
10511 bp
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UTR
Exon/CDS
Intron
Rorug06G0001100.1

Sequence Viewer

Length: 291 bp
ATGGCTACTGAAAAATTGGGTATCAAGATTGAAAAGAATCCTCCCGAGTCCACGCTCACCCAGCTCGGCGTCAGGAATTGGCCTAAGTGGAGTTGCCCTCCAAGCAAGTTTCCATGGACGTACAGTGACAAAGAGACTTGCTATCTGTTGGAAGGAAAGGTGAAGGTTACTCCTGCTGGATCAAAAGAGTCGGTTGAGATTGGTGGTGGTGACTTGGTGGAATTGCCTAAAGGAATGAGCTGCACGTGGGATGTGTCAGTGGGGGTAGACAAGCACTATAACTTCGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

96

Amino Acids

10.6

Weight (kDa)

6.56

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 20 - 93 5.2e-29 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 267
AclWI GGATC 1 cut(s) 187
AcvI CACGTG 1 cut(s) 246
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 1 cut(s) 122
AgsI TTSAA 1 cut(s) 32
AluBI AGCT 2 cut(s) 64, 240
AluI AGCT 2 cut(s) 64, 240
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 1 cut(s) 187
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 1 cut(s) 80
ApeKI GCWGC 1 cut(s) 240
AsuHPI GGTGA 3 cut(s) 49, 172, 221
AvaI CYCGRG 1 cut(s) 44
BbrPI CACGTG 1 cut(s) 246
BbvI GCAGC 1 cut(s) 227
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 1 cut(s) 289
BisI GCNGC 1 cut(s) 241
BlsI GCNGC 1 cut(s) 242
BmeT110I CYCGRG 1 cut(s) 44
BplI GAGNNNNNCTC 2 cut(s) 82, 114
BsaAI YACGTR 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 113
BseGI GGATG 1 cut(s) 256
BseXI GCAGC 1 cut(s) 227
BseYI CCCAGC 1 cut(s) 60
BsgI GTGCAG 1 cut(s) 226
BshFI GGCC 1 cut(s) 82
BsiHKCI CYCGRG 1 cut(s) 44
BsmAI GTCTC 1 cut(s) 128
BsnI GGCC 1 cut(s) 82
BsoBI CYCGRG 1 cut(s) 44
Bsp143I GATC 1 cut(s) 179
Bsp19I CCATGG 1 cut(s) 113
BspANI GGCC 1 cut(s) 82
BspPI GGATC 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 113
BssMI GATC 1 cut(s) 179
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 113
Bst4CI ACNGT 1 cut(s) 125
BstACI GRCGYC 1 cut(s) 69
BstBAI YACGTR 1 cut(s) 246
BstDEI CTNAG 1 cut(s) 84
BstDSI CCRYGG 1 cut(s) 113
BstF5I GGATG 1 cut(s) 256
BstKTI GATC 1 cut(s) 182
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 1 cut(s) 179
BstMWI GCNNNNNNNGC 2 cut(s) 61, 102
BstV1I GCAGC 1 cut(s) 227
BsuRI GGCC 1 cut(s) 82
BtgI CCRYGG 1 cut(s) 113
BtsCI GGATG 1 cut(s) 256
BtsIMutI CAGTG 2 cut(s) 130, 264
CseI GACGC 1 cut(s) 58
Csp6I GTAC 1 cut(s) 121
CviAII CATG 1 cut(s) 114
CviJI RGCY 5 cut(s) 5, 64, 82, 240, 288
CviKI_1 RGCY 5 cut(s) 5, 64, 82, 240, 288
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 1 cut(s) 84
DpnI GATC 1 cut(s) 181
DpnII GATC 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 113
Eco72I CACGTG 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 44
EcoT14I CCWWGG 1 cut(s) 113
ErhI CCWWGG 1 cut(s) 113
FaeI CATG 1 cut(s) 117
FaiI YATR 2 cut(s) 115, 279
FatI CATG 1 cut(s) 113
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 1 cut(s) 241
FokI GGATG 1 cut(s) 263
Fsp4HI GCNGC 1 cut(s) 241
FspBI CTAG 1 cut(s) 289
GluI GCNGC 1 cut(s) 241
GsaI CCCAGC 1 cut(s) 64
HaeIII GGCC 1 cut(s) 82
HgaI GACGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 1 cut(s) 117
HinfI GANTC 3 cut(s) 37, 47, 188
HphI GGTGA 3 cut(s) 49, 172, 221
Hpy166II GTNNAC 2 cut(s) 51, 268
Hpy188III TCNNGA 3 cut(s) 25, 44, 73
Hpy8I GTNNAC 2 cut(s) 51, 268
HpyAV CCTTC 2 cut(s) 146, 157
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4IV ACGT 2 cut(s) 119, 245
HpyCH4V TGCA 1 cut(s) 243
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 102
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 2 cut(s) 119, 245
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 1 cut(s) 117
Kzo9I GATC 1 cut(s) 179
LpnPI CCDG 4 cut(s) 58, 74, 162, 186
Lsp1109I GCAGC 1 cut(s) 227
MaeI CTAG 1 cut(s) 289
MaeII ACGT 2 cut(s) 119, 245
MaeIII GTNAC 3 cut(s) 125, 166, 209
MalI GATC 1 cut(s) 181
MboI GATC 1 cut(s) 179
MluCI AATT 3 cut(s) 14, 76, 221
MlyI GAGTC 2 cut(s) 56, 197
MmeI TCCRAC 1 cut(s) 129
MnlI CCTC 2 cut(s) 51, 108
MwoI GCNNNNNNNGC 2 cut(s) 61, 102
NcoI CCATGG 1 cut(s) 113
NdeII GATC 1 cut(s) 179
NlaIII CATG 1 cut(s) 117
NmeAIII GCCGAG 1 cut(s) 45
NmuCI GTSAC 2 cut(s) 125, 209
PfeI GAWTC 1 cut(s) 37
PkrI GCNGC 1 cut(s) 242
PleI GAGTC 2 cut(s) 55, 196
PmaCI CACGTG 1 cut(s) 246
PmlI CACGTG 1 cut(s) 246
PpsI GAGTC 2 cut(s) 55, 196
Ppu21I YACGTR 1 cut(s) 246
PspCI CACGTG 1 cut(s) 246
PspFI CCCAGC 1 cut(s) 60
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
SatI GCNGC 1 cut(s) 241
Sau3AI GATC 1 cut(s) 179
SchI GAGTC 2 cut(s) 56, 197
SetI ASST 6 cut(s) 66, 122, 162, 168, 242, 248
Sse9I AATT 3 cut(s) 14, 76, 221
SspMI CTAG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 113
TaaI ACNGT 1 cut(s) 125
TaiI ACGT 2 cut(s) 122, 248
TasI AATT 3 cut(s) 14, 76, 221
TfiI GAWTC 1 cut(s) 37
TscAI CASTG 2 cut(s) 130, 264
TseFI GTSAC 2 cut(s) 125, 209
TseI GCWGC 1 cut(s) 240
Tsp45I GTSAC 2 cut(s) 125, 209
TspRI CASTG 2 cut(s) 130, 264
XmiI GTMKAC 1 cut(s) 267
XspI CTAG 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.