Rroxscaffold_177G00434270

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000177
OG0010523
Physical Location & Seq
Reverse (-)
236641 .. 237813
1173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_177G00434270.1

Sequence Viewer

Length: 1173 bp
ATGGGCAATGGAACCAGTGTTCAAGCAAAGCCAGAGAAACGACCCGTTTTGGAGCGAAAGACATCCTTTGCTAAGAATCATAGGATTGTTCCCATCAATTCCGAGCCAAGAATTTTTGAAGAGTTGATGAGATCGTGTCAGGCGGCTTGCAAAGATGACAAAGAGTTGCAAGACTTCGACGCCGAACTCAGCTCCGGCGCCGAAGAAGTCGTCCGCAGTACCGGTCTTGATAGGTTCAAGCAATGCCTAAAGTACCTGACGCAATCCAACGAGAAGATTGTGAGGGTAATCTTATCATGCAAAAAACAGATAGTTGAGGCAGAGGGTGTGCAGGCGAGGATGGTGATGATGGCGAATGAGCAGCTCTTGGATCAATTTTTGATCAAGGAGTACTTCAACAATAGCTTGCAGACACTTCAACTCTGCAGATTGGAATTGCAGGACTTTATCAACTCGGCTCGTGAGGTCCATTCATGCATTACAGATGTGGTACTTGATTGGGAAGTGTCGAAGAAGCTCAACGAGTCTCAGAAGATGTATCACCGTTCTGTGGAAGCAGTTGTTGCCAAGGTGAACTCTCTGCGCGACCACCATAGAGACATGCTTGACAAGTTGAAAGATCGAATTCAGAAGTTTGATAAGAAGCTGGAGCTTACTCGTACTTTAAAGAAGGTCGTAAATGTGGTGTTCATGGGTATGTTGTCTGGTCTGTTTGTTTGCACCATCATGGCGGCGGCTATGGCTGCTCCGCCAGTTGCGTCAGCCTTGACCTCTCATCCATGTATTGCCGCAGTTATTGCTGGTGGTGCCGTTGGTTATGCTTTTTCGTCAGCATTAAAGGAGGGACAACACTGGCTTCTGACTTTGATTCAAGGTTATGAATCAACTCTGGATGCACATACGGGTGTAATTAAGTCGATGGAAGCCGGAACAAGAGACGCCATTAAGGAGTTGGATGACATCATGTGTCATGTTAATGAGGTCATTGCAAAAGGGAATGATCCAGTGTTGTCCAATTTAAGCTCCGCAAATGATGAGGGAAGAGTGGATGTGGTTGATCAGGAAGCAGTGACGTCTATTCTGGAAAAGCTGGCAGAGTTTGGCCAGGAGATTGAGGAACTGGACGAGAAAAAGAGAGCAGTGCCTCCGGGTTATAGAGACTGCAAGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

390

Amino Acids

43.55

Weight (kDa)

5.94

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 67 - 377 5.4e-24 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1076
AccB1I GGYRCC 2 cut(s) 197, 806
AccII CGCG 1 cut(s) 585
AciI CCGC 7 cut(s) 143, 214, 731, 734, 749, 789, 1026
AclWI GGATC 2 cut(s) 378, 995
AcoI YGGCCR 1 cut(s) 1102
AcsI RAATTY 2 cut(s) 111, 624
AcyI GRCGYC 4 cut(s) 180, 198, 939, 1073
AfaI GTAC 5 cut(s) 220, 254, 392, 492, 661
AfiI CCNNNNNNNGG 1 cut(s) 550
AgeI ACCGGT 1 cut(s) 221
AgsI TTSAA 7 cut(s) 23, 119, 238, 397, 419, 616, 872
AjnI CCWGG 1 cut(s) 1104
AloI GAACNNNNNNTCC 1 cut(s) 35
AluBI AGCT 8 cut(s) 192, 364, 405, 517, 646, 652, 1023, 1090
AluI AGCT 8 cut(s) 192, 364, 405, 517, 646, 652, 1023, 1090
Alw26I GTCTC 4 cut(s) 531, 591, 930, 1152
AlwI GGATC 2 cut(s) 378, 995
AoxI GGCC 1 cut(s) 1102
ApeKI GCWGC 2 cut(s) 361, 743
ApoI RAATTY 2 cut(s) 111, 624
ArsI GACNNNNNNTTYG 2 cut(s) 195, 227
AsiGI ACCGGT 1 cut(s) 221
AspLEI GCGC 2 cut(s) 200, 585
AspS9I GGNCC 1 cut(s) 466
AsuC2I CCSGG 1 cut(s) 1149
AsuHPI GGTGA 3 cut(s) 355, 533, 583
AvaII GGWCC 1 cut(s) 466
BalI TGGCCA 1 cut(s) 1104
BanI GGYRCC 2 cut(s) 197, 806
BauI CACGAG 1 cut(s) 459
BbvI GCAGC 2 cut(s) 373, 730
BccI CCATC 5 cut(s) 101, 334, 343, 731, 913
BceAI ACGGC 1 cut(s) 794
BciT130I CCWGG 1 cut(s) 1106
BclI TGATCA 2 cut(s) 381, 1057
BcnI CCSGG 1 cut(s) 1149
BcoDI GTCTC 4 cut(s) 531, 591, 930, 1152
BfmI CTRYAG 1 cut(s) 424
BfoI RGCGCY 1 cut(s) 201
BisI GCNGC 6 cut(s) 144, 362, 732, 735, 744, 789
BlsI GCNGC 6 cut(s) 145, 363, 733, 736, 745, 790
BmcAI AGTACT 1 cut(s) 392
Bme1390I CCNGG 2 cut(s) 1106, 1149
Bme18I GGWCC 1 cut(s) 466
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 3 cut(s) 13, 199, 808
BmrFI CCNGG 2 cut(s) 1106, 1149
BmsI GCATC 1 cut(s) 883
BpmI CTGGAG 1 cut(s) 668
BpuMI CCSGG 1 cut(s) 1149
BsaHI GRCGYC 4 cut(s) 180, 198, 939, 1073
BsaJI CCNNGG 1 cut(s) 567
BsaWI WCCGGW 1 cut(s) 221
Bsc4I CCNNNNNNNGG 1 cut(s) 550
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 5 cut(s) 15, 752, 857, 1004, 1125
Bse3DI GCAATG 3 cut(s) 13, 248, 984
BseBI CCWGG 1 cut(s) 1106
BseDI CCNNGG 1 cut(s) 567
BseGI GGATG 6 cut(s) 62, 345, 775, 898, 961, 1054
BseLI CCNNNNNNNGG 1 cut(s) 550
BseMI GCAATG 3 cut(s) 13, 248, 984
BseMII CTCAG 2 cut(s) 202, 542
BseNI ACTGG 5 cut(s) 15, 752, 857, 1004, 1125
BseXI GCAGC 2 cut(s) 373, 730
BsgI GTGCAG 1 cut(s) 350
Bsh1236I CGCG 1 cut(s) 585
BshFI GGCC 1 cut(s) 1104
BshNI GGYRCC 2 cut(s) 197, 806
BshTI ACCGGT 1 cut(s) 221
BsiSI CCGG 4 cut(s) 195, 222, 927, 1148
BslFI GGGAC 1 cut(s) 858
BslI CCNNNNNNNGG 1 cut(s) 550
BsmAI GTCTC 4 cut(s) 531, 591, 930, 1152
BsmBI CGTCTC 1 cut(s) 930
BsmFI GGGAC 1 cut(s) 858
BsnI GGCC 1 cut(s) 1104
Bsp143I GATC 6 cut(s) 131, 370, 381, 619, 1000, 1057
BspACI CCGC 7 cut(s) 143, 214, 731, 734, 749, 789, 1026
BspANI GGCC 1 cut(s) 1104
BspCNI CTCAG 2 cut(s) 201, 541
BspFNI CGCG 1 cut(s) 585
BspLI GGNNCC 3 cut(s) 13, 199, 808
BspMAI CTGCAG 1 cut(s) 428
BspPI GGATC 2 cut(s) 378, 995
BspT107I GGYRCC 2 cut(s) 197, 806
BsrDI GCAATG 3 cut(s) 13, 248, 984
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 5 cut(s) 15, 752, 857, 1004, 1125
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 1 cut(s) 567
BssMI GATC 6 cut(s) 131, 370, 381, 619, 1000, 1057
BssNI GRCGYC 4 cut(s) 180, 198, 939, 1073
BssSI CACGAG 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 459
Bst2UI CCWGG 1 cut(s) 1106
Bst4CI ACNGT 1 cut(s) 545
Bst6I CTCTTC 2 cut(s) 114, 1036
BstACI GRCGYC 4 cut(s) 180, 198, 939, 1073
BstAPI GCANNNNNTGC 2 cut(s) 563, 797
BstC8I GCNNGC 4 cut(s) 148, 333, 407, 1092
BstDEI CTNAG 3 cut(s) 72, 188, 528
BstF5I GGATG 6 cut(s) 62, 345, 775, 898, 961, 1054
BstFNI CGCG 1 cut(s) 585
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 2 cut(s) 200, 585
BstKTI GATC 6 cut(s) 134, 373, 384, 622, 1003, 1060
BstMAI GTCTC 4 cut(s) 531, 591, 930, 1152
BstMBI GATC 6 cut(s) 131, 370, 381, 619, 1000, 1057
BstMWI GCNNNNNNNGC 5 cut(s) 563, 740, 743, 797, 806
BstNI CCWGG 1 cut(s) 1106
BstNSI RCATGY 1 cut(s) 604
BstSCI CCNGG 2 cut(s) 1104, 1147
BstSFI CTRYAG 1 cut(s) 424
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 2 cut(s) 373, 730
BsuRI GGCC 1 cut(s) 1104
BtsCI GGATG 6 cut(s) 62, 345, 775, 898, 961, 1054
BtsI GCAGTG 2 cut(s) 1074, 1146
BtsIMutI CAGTG 5 cut(s) 22, 850, 1011, 1074, 1146
Cac8I GCNNGC 4 cut(s) 148, 333, 407, 1092
CfoI GCGC 2 cut(s) 200, 585
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 1 cut(s) 466
CseI GACGC 4 cut(s) 188, 268, 747, 947
Csp6I GTAC 5 cut(s) 219, 253, 391, 491, 660
CspAI ACCGGT 1 cut(s) 221
CviAII CATG 8 cut(s) 297, 474, 601, 691, 727, 780, 964, 971
CviQI GTAC 5 cut(s) 219, 253, 391, 491, 660
DdeI CTNAG 3 cut(s) 72, 188, 528
DinI GGCGCC 1 cut(s) 199
DpnI GATC 6 cut(s) 133, 372, 383, 621, 1002, 1059
DpnII GATC 6 cut(s) 131, 370, 381, 619, 1000, 1057
DraI TTTAAA 1 cut(s) 666
EaeI YGGCCR 1 cut(s) 1102
Eam1104I CTCTTC 2 cut(s) 114, 1036
EarI CTCTTC 2 cut(s) 114, 1036
EciI GGCGGA 1 cut(s) 738
Eco130I CCWWGG 1 cut(s) 567
Eco47I GGWCC 1 cut(s) 466
EcoRI GAATTC 1 cut(s) 624
EcoRII CCWGG 1 cut(s) 1104
EcoT14I CCWWGG 1 cut(s) 567
EcoT22I ATGCAT 1 cut(s) 479
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 567
Esp3I CGTCTC 1 cut(s) 930
FaeI CATG 8 cut(s) 300, 477, 604, 694, 730, 783, 967, 974
FalI AAGNNNNNCTT 4 cut(s) 50, 82, 377, 409
FaqI GGGAC 1 cut(s) 858
FatI CATG 8 cut(s) 296, 473, 600, 690, 726, 779, 963, 970
FbaI TGATCA 2 cut(s) 381, 1057
Fnu4HI GCNGC 6 cut(s) 144, 362, 732, 735, 744, 789
FokI GGATG 6 cut(s) 49, 352, 762, 905, 968, 1061
Fsp4HI GCNGC 6 cut(s) 144, 362, 732, 735, 744, 789
GlaI GCGC 2 cut(s) 199, 584
GluI GCNGC 6 cut(s) 144, 362, 732, 735, 744, 789
GsuI CTGGAG 1 cut(s) 668
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 1 cut(s) 1104
HapII CCGG 4 cut(s) 195, 222, 927, 1148
HgaI GACGC 4 cut(s) 188, 268, 747, 947
HhaI GCGC 2 cut(s) 200, 585
Hin1I GRCGYC 4 cut(s) 180, 198, 939, 1073
Hin1II CATG 8 cut(s) 300, 477, 604, 694, 730, 783, 967, 974
Hin6I GCGC 2 cut(s) 198, 583
HinP1I GCGC 2 cut(s) 198, 583
HinfI GANTC 4 cut(s) 76, 524, 868, 881
HpaII CCGG 4 cut(s) 195, 222, 927, 1148
HphI GGTGA 3 cut(s) 355, 533, 583
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 4 cut(s) 103, 531, 630, 861
Hpy188III TCNNGA 5 cut(s) 227, 461, 890, 1061, 1082
Hpy8I GTNNAC 1 cut(s) 574
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 1 cut(s) 664
HpyCH4III ACNGT 1 cut(s) 545
HpyCH4IV ACGT 1 cut(s) 1073
HpyF10VI GCNNNNNNNGC 5 cut(s) 563, 740, 743, 797, 806
HpyF3I CTNAG 3 cut(s) 72, 188, 528
HpySE526I ACGT 1 cut(s) 1073
Hsp92I GRCGYC 4 cut(s) 180, 198, 939, 1073
Hsp92II CATG 8 cut(s) 300, 477, 604, 694, 730, 783, 967, 974
HspAI GCGC 2 cut(s) 198, 583
KasI GGCGCC 1 cut(s) 197
Ksp22I TGATCA 2 cut(s) 381, 1057
Kzo9I GATC 6 cut(s) 131, 370, 381, 619, 1000, 1057
LmnI GCTCC 5 cut(s) 52, 197, 649, 751, 1028
Lsp1109I GCAGC 2 cut(s) 373, 730
LweI GCATC 1 cut(s) 883
MaeII ACGT 1 cut(s) 1073
MaeIII GTNAC 1 cut(s) 1069
MalI GATC 6 cut(s) 133, 372, 383, 621, 1002, 1059
MboI GATC 6 cut(s) 131, 370, 381, 619, 1000, 1057
MboII GAAGA 6 cut(s) 131, 215, 286, 523, 544, 1053
MlsI TGGCCA 1 cut(s) 1104
MluCI AATT 7 cut(s) 97, 111, 374, 434, 624, 909, 1015
MluNI TGGCCA 1 cut(s) 1104
Mly113I GGCGCC 1 cut(s) 198
MlyI GAGTC 1 cut(s) 533
MmeI TCCRAC 2 cut(s) 291, 933
Mox20I TGGCCA 1 cut(s) 1104
Mph1103I ATGCAT 1 cut(s) 479
MscI TGGCCA 1 cut(s) 1104
MseI TTAA 6 cut(s) 665, 836, 912, 945, 975, 1019
MslI CAYNNNNRTG 4 cut(s) 695, 725, 903, 975
Msp20I TGGCCA 1 cut(s) 1104
MspI CCGG 4 cut(s) 195, 222, 927, 1148
MspR9I CCNGG 2 cut(s) 1106, 1149
MvaI CCWGG 1 cut(s) 1106
MvnI CGCG 1 cut(s) 585
MwoI GCNNNNNNNGC 5 cut(s) 563, 740, 743, 797, 806
NarI GGCGCC 1 cut(s) 198
NciI CCSGG 1 cut(s) 1149
NdeII GATC 6 cut(s) 131, 370, 381, 619, 1000, 1057
NlaIII CATG 8 cut(s) 300, 477, 604, 694, 730, 783, 967, 974
NlaIV GGNNCC 3 cut(s) 13, 199, 808
NmeAIII GCCGAG 1 cut(s) 434
NmuCI GTSAC 1 cut(s) 1069
NsiI ATGCAT 1 cut(s) 479
NspI RCATGY 1 cut(s) 604
PfeI GAWTC 3 cut(s) 76, 868, 881
PinAI ACCGGT 1 cut(s) 221
PkrI GCNGC 6 cut(s) 145, 363, 733, 736, 745, 790
PleI GAGTC 1 cut(s) 532
PluTI GGCGCC 1 cut(s) 201
PpsI GAGTC 1 cut(s) 532
Psp6I CCWGG 1 cut(s) 1104
PspGI CCWGG 1 cut(s) 1104
PspN4I GGNNCC 3 cut(s) 13, 199, 808
PspPI GGNCC 1 cut(s) 466
PstI CTGCAG 1 cut(s) 428
RsaI GTAC 5 cut(s) 220, 254, 392, 492, 661
RsaNI GTAC 5 cut(s) 219, 253, 391, 491, 660
RseI CAYNNNNRTG 4 cut(s) 695, 725, 903, 975
SaqAI TTAA 6 cut(s) 665, 836, 912, 945, 975, 1019
SatI GCNGC 6 cut(s) 144, 362, 732, 735, 744, 789
Sau3AI GATC 6 cut(s) 131, 370, 381, 619, 1000, 1057
Sau96I GGNCC 1 cut(s) 466
ScaI AGTACT 1 cut(s) 392
SchI GAGTC 1 cut(s) 533
ScrFI CCNGG 2 cut(s) 1106, 1149
SfaNI GCATC 1 cut(s) 883
SfcI CTRYAG 1 cut(s) 424
SfoI GGCGCC 1 cut(s) 199
SinI GGWCC 1 cut(s) 466
SmiMI CAYNNNNRTG 4 cut(s) 695, 725, 903, 975
Sse9I AATT 7 cut(s) 97, 111, 374, 434, 624, 909, 1015
SsiI CCGC 7 cut(s) 143, 214, 731, 734, 749, 789, 1026
SspDI GGCGCC 1 cut(s) 197
StyD4I CCNGG 2 cut(s) 1104, 1147
StyI CCWWGG 1 cut(s) 567
TaaI ACNGT 1 cut(s) 545
TaiI ACGT 1 cut(s) 1076
TaqI TCGA 4 cut(s) 177, 509, 622, 917
TasI AATT 7 cut(s) 97, 111, 374, 434, 624, 909, 1015
TatI WGTACW 1 cut(s) 390
TauI GCSGC 4 cut(s) 146, 734, 737, 791
TfiI GAWTC 3 cut(s) 76, 868, 881
Tru1I TTAA 6 cut(s) 665, 836, 912, 945, 975, 1019
Tru9I TTAA 6 cut(s) 665, 836, 912, 945, 975, 1019
TscAI CASTG 5 cut(s) 22, 857, 1011, 1074, 1146
TseFI GTSAC 1 cut(s) 1069
TseI GCWGC 2 cut(s) 361, 743
Tsp45I GTSAC 1 cut(s) 1069
TspDTI ATGAA 3 cut(s) 462, 679, 894
TspRI CASTG 5 cut(s) 22, 857, 1011, 1074, 1146
VpaK11BI GGWCC 1 cut(s) 466
XapI RAATTY 2 cut(s) 111, 624
XceI RCATGY 1 cut(s) 604
XcmI CCANNNNNNNNNTGG 1 cut(s) 949
ZraI GACGTC 1 cut(s) 1074
ZrmI AGTACT 1 cut(s) 392
Zsp2I ATGCAT 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.