Rroxscaffold_177G00434180

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000177
OG0010523
Physical Location & Seq
Reverse (-)
115064 .. 117490
2427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_177G00434180.1

Sequence Viewer

Length: 1170 bp
ATGGGCGGTGCTCAAGCAAAGGCTGCAAAGCCAGAGAGAGGACGAGCCGGTTTGGAGCGAAAGACATCCTTTGCTAAGAATCAGAGCGTTGTTCCCATCAATGCCGAACCAAGGCCAACTGACGACCTCTGCAAGGATTTTGAAGACTTGATGAGATCGTATCAGGCGGATTGCAAAGATGACAAAGACTTGCAAGACTTCGACGCCGCACTTGGCTCCAGCGCCAAAGAAGTCATCTGCAGTACCGGTTTTGATCAGCTTAAGCAATGCATAAAGTGTCTGACCGATTCCGACGAGAAGATGAAGGAGGTAATCTTGTCATGCATAACACAGATACTTGAGGCAGAGGGTGTGCAGGTGAGGGTGGTGATGACGGGGAATGAGAACCTCTTAGATGATTTGATCGGCGAGTACCTCGAGAATAGCGAGCAGACGCTTGACCTCTGTGGATTGGAATTGCAAGATTTCATCGACTCGGCTCGTGTGGCCCATTCATGCATTAAAGATGTGCTTGAGAGGGGAGTGTCGAAGAAGCTCAACGAGTCTGTGAGGAAGTATCATGGTTCTGCGGAAGCAGTTCTTGCAAAGGTGAAATCTCTGCTCGACCAACATAGAGACATGCTTGACAAGTTGAAAGATCGAATTCAGAAGCTTGATAAGAAGCTGGAGCTTAATCGTACTTTAAAAAAGGTCGTAAATGTGGTGTTCTTAGGTATGTTATTTGGTCTGTTTGTTTGCACCATCATGGCAGCGGCTATGGCTGCTCCGCCAGTTGCATCAGCCTTGACTTGTACTCCAAGTATTTACTACGTTGTTGCTGGTGGTGCCGTTGGTTATGGTTTTTCGTCGATATTAAAAGAGGGACAACGCCGGCTTCTCACTTCGATTCAAGGTAATGTATCAACTCTGGAGGCACATAAGGGTGTAATTAAGTCTATGGAAGCCGGAACCGGACACGCCATTAAAGAGTTGGATGACATCATGTGTCATGTTAATAAGGTCATTGCAAAGGGGAATGATCCAGTGTTGTCCAATTTAAGCTCTGCAAATGATGAGGGAAGAGTAGATGTGGAAATTGATGAGAAAGAGATTATTGAAAAAAGCGGACAGAGTTTCACAAAGCAATTGAGGAATTGGACAAGAAAAGAAAGCAGTGCGTCGAGGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

389

Amino Acids

42.69

Weight (kDa)

5.84

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 191 - 382 6.3e-17 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 346
Acc36I ACCTGC 1 cut(s) 346
AccB1I GGYRCC 1 cut(s) 824
AciI CCGC 7 cut(s) 6, 167, 207, 569, 752, 767, 1104
AclWI GGATC 1 cut(s) 1013
AcsI RAATTY 1 cut(s) 642
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 4 cut(s) 244, 413, 679, 793
AfiI CCNNNNNNNGG 3 cut(s) 38, 111, 133
AflII CTTAAG 1 cut(s) 260
AgeI ACCGGT 1 cut(s) 245
AgsI TTSAA 4 cut(s) 143, 634, 890, 1097
AluBI AGCT 6 cut(s) 259, 535, 652, 664, 670, 1041
AluI AGCT 6 cut(s) 259, 535, 652, 664, 670, 1041
Alw21I GWGCWC 1 cut(s) 13
Alw26I GTCTC 1 cut(s) 609
AlwI GGATC 1 cut(s) 1013
Ama87I CYCGRG 1 cut(s) 416
AoxI GGCC 2 cut(s) 113, 486
ApeKI GCWGC 3 cut(s) 23, 749, 761
ApoI RAATTY 1 cut(s) 642
AsiGI ACCGGT 1 cut(s) 245
Asp700I GAANNNNTTC 1 cut(s) 576
AspLEI GCGC 1 cut(s) 224
AspS9I GGNCC 1 cut(s) 487
AsuHPI GGTGA 3 cut(s) 370, 379, 601
AvaI CYCGRG 1 cut(s) 416
BanI GGYRCC 1 cut(s) 824
BauI CACGAG 1 cut(s) 480
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 13
BbvI GCAGC 3 cut(s) 10, 748, 761
BccI CCATC 2 cut(s) 104, 749
BceAI ACGGC 1 cut(s) 812
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 1 cut(s) 609
BfmI CTRYAG 1 cut(s) 238
BfoI RGCGCY 1 cut(s) 225
BfrI CTTAAG 1 cut(s) 260
BfuAI ACCTGC 1 cut(s) 346
BisI GCNGC 5 cut(s) 24, 207, 750, 753, 762
BlsI GCNGC 5 cut(s) 25, 208, 751, 754, 763
BmeT110I CYCGRG 1 cut(s) 416
BmgT120I GGNCC 1 cut(s) 487
BmiI GGNNCC 3 cut(s) 217, 826, 949
BmsI GCATC 1 cut(s) 785
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 3 cut(s) 202, 686, 929
BpuEI CTTGAG 2 cut(s) 359, 533
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 110
BsaWI WCCGGW 2 cut(s) 245, 950
BsaXI ACNNNNNCTCC 2 cut(s) 778, 808
Bsc4I CCNNNNNNNGG 3 cut(s) 38, 111, 133
Bse118I RCCGGY 3 cut(s) 47, 245, 870
Bse1I ACTGG 2 cut(s) 770, 1022
Bse3DI GCAATG 2 cut(s) 272, 1002
BseDI CCNNGG 1 cut(s) 110
BseGI GGATG 2 cut(s) 65, 979
BseLI CCNNNNNNNGG 3 cut(s) 38, 111, 133
BseMI GCAATG 2 cut(s) 272, 1002
BseNI ACTGG 2 cut(s) 770, 1022
BseXI GCAGC 3 cut(s) 10, 748, 761
BsgI GTGCAG 1 cut(s) 374
BshFI GGCC 2 cut(s) 115, 488
BshNI GGYRCC 1 cut(s) 824
BshTI ACCGGT 1 cut(s) 245
BsiHKAI GWGCWC 1 cut(s) 13
BsiHKCI CYCGRG 1 cut(s) 416
BsiSI CCGG 5 cut(s) 48, 246, 871, 945, 951
BslFI GGGAC 1 cut(s) 876
BslI CCNNNNNNNGG 3 cut(s) 38, 111, 133
BsmAI GTCTC 1 cut(s) 609
BsmFI GGGAC 1 cut(s) 876
BsnI GGCC 2 cut(s) 115, 488
BsoBI CYCGRG 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 13
Bsp143I GATC 5 cut(s) 155, 253, 402, 637, 1018
BspACI CCGC 7 cut(s) 6, 167, 207, 569, 752, 767, 1104
BspANI GGCC 2 cut(s) 115, 488
BspLI GGNNCC 3 cut(s) 217, 826, 949
BspMAI CTGCAG 1 cut(s) 242
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 1 cut(s) 1013
BspT107I GGYRCC 1 cut(s) 824
BspTI CTTAAG 1 cut(s) 260
BsrDI GCAATG 2 cut(s) 272, 1002
BsrFI RCCGGY 3 cut(s) 47, 245, 870
BsrI ACTGG 2 cut(s) 770, 1022
BssAI RCCGGY 3 cut(s) 47, 245, 870
BssECI CCNNGG 1 cut(s) 110
BssMI GATC 5 cut(s) 155, 253, 402, 637, 1018
BssNI GRCGYC 1 cut(s) 204
BssSI CACGAG 1 cut(s) 480
BssT1I CCWWGG 1 cut(s) 110
Bst2BI CACGAG 1 cut(s) 480
Bst6I CTCTTC 1 cut(s) 1054
BstACI GRCGYC 1 cut(s) 204
BstAFI CTTAAG 1 cut(s) 260
BstAPI GCANNNNNTGC 2 cut(s) 23, 581
BstC8I GCNNGC 2 cut(s) 428, 872
BstDEI CTNAG 3 cut(s) 75, 391, 709
BstENI CCTNNNNNAGG 1 cut(s) 131
BstF5I GGATG 2 cut(s) 65, 979
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 5 cut(s) 158, 256, 405, 640, 1021
BstMAI GTCTC 1 cut(s) 609
BstMBI GATC 5 cut(s) 155, 253, 402, 637, 1018
BstMWI GCNNNNNNNGC 6 cut(s) 23, 485, 581, 758, 761, 824
BstNSI RCATGY 1 cut(s) 622
BstSFI CTRYAG 1 cut(s) 238
BstV1I GCAGC 3 cut(s) 10, 748, 761
BstV2I GAAGAC 1 cut(s) 150
BsuRI GGCC 2 cut(s) 115, 488
BtsCI GGATG 2 cut(s) 65, 979
BtsI GCAGTG 1 cut(s) 1159
BtsIMutI CAGTG 2 cut(s) 1029, 1159
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 2 cut(s) 428, 872
CfoI GCGC 1 cut(s) 224
Cfr10I RCCGGY 3 cut(s) 47, 245, 870
Cfr13I GGNCC 1 cut(s) 487
CseI GACGC 3 cut(s) 212, 442, 1146
Csp6I GTAC 4 cut(s) 243, 412, 678, 792
CspAI ACCGGT 1 cut(s) 245
CviAII CATG 7 cut(s) 321, 495, 560, 619, 745, 982, 989
CviQI GTAC 4 cut(s) 243, 412, 678, 792
DdeI CTNAG 3 cut(s) 75, 391, 709
DpnI GATC 5 cut(s) 157, 255, 404, 639, 1020
DpnII GATC 5 cut(s) 155, 253, 402, 637, 1018
DraI TTTAAA 1 cut(s) 684
Eam1104I CTCTTC 1 cut(s) 1054
EarI CTCTTC 1 cut(s) 1054
EciI GGCGGA 2 cut(s) 182, 756
Eco130I CCWWGG 1 cut(s) 110
Eco88I CYCGRG 1 cut(s) 416
EcoNI CCTNNNNNAGG 1 cut(s) 131
EcoRI GAATTC 1 cut(s) 642
EcoT14I CCWWGG 1 cut(s) 110
EcoT22I ATGCAT 3 cut(s) 272, 326, 500
ErhI CCWWGG 1 cut(s) 110
FaeI CATG 7 cut(s) 324, 498, 563, 622, 748, 985, 992
FalI AAGNNNNNCTT 6 cut(s) 53, 85, 495, 527, 564, 596
FaqI GGGAC 1 cut(s) 876
FatI CATG 7 cut(s) 320, 494, 559, 618, 744, 981, 988
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 5 cut(s) 24, 207, 750, 753, 762
FokI GGATG 2 cut(s) 52, 986
Fsp4HI GCNGC 5 cut(s) 24, 207, 750, 753, 762
GlaI GCGC 1 cut(s) 223
GluI GCNGC 5 cut(s) 24, 207, 750, 753, 762
GsuI CTGGAG 3 cut(s) 202, 686, 929
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 2 cut(s) 115, 488
HapII CCGG 5 cut(s) 48, 246, 871, 945, 951
HgaI GACGC 3 cut(s) 212, 442, 1146
HhaI GCGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 7 cut(s) 324, 498, 563, 622, 748, 985, 992
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 5 cut(s) 79, 287, 473, 542, 886
HpaII CCGG 5 cut(s) 48, 246, 871, 945, 951
HphI GGTGA 3 cut(s) 370, 379, 601
Hpy188I TCNGA 4 cut(s) 84, 282, 292, 648
Hpy188III TCNNGA 2 cut(s) 418, 908
Hpy99I CGWCG 4 cut(s) 206, 296, 850, 1162
HpyAV CCTTC 1 cut(s) 298
HpyCH4IV ACGT 1 cut(s) 810
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 485, 581, 758, 761, 824
HpyF3I CTNAG 3 cut(s) 75, 391, 709
HpySE526I ACGT 1 cut(s) 810
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 7 cut(s) 324, 498, 563, 622, 748, 985, 992
HspAI GCGC 1 cut(s) 222
KroI GCCGGC 1 cut(s) 870
KroNI GCCGGC 1 cut(s) 872
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 5 cut(s) 155, 253, 402, 637, 1018
LmnI GCTCC 4 cut(s) 55, 221, 667, 769
Lsp1109I GCAGC 3 cut(s) 10, 748, 761
LweI GCATC 1 cut(s) 785
MaeII ACGT 1 cut(s) 810
MalI GATC 5 cut(s) 157, 255, 404, 639, 1020
MboI GATC 5 cut(s) 155, 253, 402, 637, 1018
MboII GAAGA 4 cut(s) 155, 310, 541, 1071
MfeI CAATTG 1 cut(s) 1124
MhlI GDGCHC 1 cut(s) 13
MluCI AATT 7 cut(s) 455, 642, 927, 1033, 1074, 1124, 1132
MlyI GAGTC 2 cut(s) 467, 551
MmeI TCCRAC 2 cut(s) 315, 951
Mph1103I ATGCAT 3 cut(s) 272, 326, 500
MroNI GCCGGC 1 cut(s) 870
MroXI GAANNNNTTC 1 cut(s) 576
MseI TTAA 9 cut(s) 261, 501, 672, 683, 854, 930, 963, 993, 1037
MslI CAYNNNNRTG 2 cut(s) 743, 921
MspA1I CMGCKG 1 cut(s) 752
MspCI CTTAAG 1 cut(s) 260
MspI CCGG 5 cut(s) 48, 246, 871, 945, 951
MunI CAATTG 1 cut(s) 1124
MwoI GCNNNNNNNGC 6 cut(s) 23, 485, 581, 758, 761, 824
NaeI GCCGGC 1 cut(s) 872
NdeII GATC 5 cut(s) 155, 253, 402, 637, 1018
NgoMIV GCCGGC 1 cut(s) 870
NlaIII CATG 7 cut(s) 324, 498, 563, 622, 748, 985, 992
NlaIV GGNNCC 3 cut(s) 217, 826, 949
NmeAIII GCCGAG 1 cut(s) 455
NsiI ATGCAT 3 cut(s) 272, 326, 500
NspI RCATGY 1 cut(s) 622
PaeR7I CTCGAG 1 cut(s) 416
PaqCI CACCTGC 1 cut(s) 346
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PdiI GCCGGC 1 cut(s) 872
PdmI GAANNNNTTC 1 cut(s) 576
PfeI GAWTC 3 cut(s) 79, 287, 886
PinAI ACCGGT 1 cut(s) 245
PkrI GCNGC 5 cut(s) 25, 208, 751, 754, 763
PleI GAGTC 2 cut(s) 467, 550
PpsI GAGTC 2 cut(s) 467, 550
PspN4I GGNNCC 3 cut(s) 217, 826, 949
PspPI GGNCC 1 cut(s) 487
PstI CTGCAG 1 cut(s) 242
RsaI GTAC 4 cut(s) 244, 413, 679, 793
RsaNI GTAC 4 cut(s) 243, 412, 678, 792
RseI CAYNNNNRTG 2 cut(s) 743, 921
SaqAI TTAA 9 cut(s) 261, 501, 672, 683, 854, 930, 963, 993, 1037
SatI GCNGC 5 cut(s) 24, 207, 750, 753, 762
Sau3AI GATC 5 cut(s) 155, 253, 402, 637, 1018
Sau96I GGNCC 1 cut(s) 487
SchI GAGTC 2 cut(s) 467, 551
SduI GDGCHC 1 cut(s) 13
SfaNI GCATC 1 cut(s) 785
SfcI CTRYAG 1 cut(s) 238
Sfr274I CTCGAG 1 cut(s) 416
SlaI CTCGAG 1 cut(s) 416
SmiMI CAYNNNNRTG 2 cut(s) 743, 921
SmlI CTYRAG 5 cut(s) 12, 260, 338, 416, 512
SmoI CTYRAG 5 cut(s) 12, 260, 338, 416, 512
Sse9I AATT 7 cut(s) 455, 642, 927, 1033, 1074, 1124, 1132
SsiI CCGC 7 cut(s) 6, 167, 207, 569, 752, 767, 1104
StyI CCWWGG 1 cut(s) 110
TaiI ACGT 1 cut(s) 813
TaqI TCGA 9 cut(s) 201, 417, 471, 527, 603, 640, 848, 884, 1160
TaqII GACCGA 1 cut(s) 299
TasI AATT 7 cut(s) 455, 642, 927, 1033, 1074, 1124, 1132
TatI WGTACW 1 cut(s) 791
TauI GCSGC 2 cut(s) 209, 755
TfiI GAWTC 3 cut(s) 79, 287, 886
Tru1I TTAA 9 cut(s) 261, 501, 672, 683, 854, 930, 963, 993, 1037
Tru9I TTAA 9 cut(s) 261, 501, 672, 683, 854, 930, 963, 993, 1037
TscAI CASTG 2 cut(s) 1029, 1159
TseI GCWGC 3 cut(s) 23, 749, 761
TspDTI ATGAA 3 cut(s) 317, 457, 483
TspRI CASTG 2 cut(s) 1029, 1159
Vha464I CTTAAG 1 cut(s) 260
XagI CCTNNNNNAGG 1 cut(s) 131
XapI RAATTY 1 cut(s) 642
XceI RCATGY 1 cut(s) 622
XcmI CCANNNNNNNNNTGG 1 cut(s) 967
XhoI CTCGAG 1 cut(s) 416
XmnI GAANNNNTTC 1 cut(s) 576
Zsp2I ATGCAT 3 cut(s) 272, 326, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.