RLG00000014394

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010523
Physical Location & Seq
Reverse (-)
52586126 .. 52587355
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014394

Sequence Viewer

Length: 1230 bp
ATGGGCAATGGAACCAGTGTTCAAGCAAAGCCAGAGAAACGACCCCTTTTGGAGCGAAAGACATCCTTTGCTAAGAATCATAGGATTGTTCCCATCAACTCCGAGCCAAGAATTTTTGAAGAGTTGATGAGATCGTGTCAGGCGGCTTGCAAAGATGACAAAGAGTTGCAAGACTTCGACGCCGAACTCAGCTCCGGCGCCGAAGAAGTCGTCCGCAGTACAGGTCTTGATCGGTTCAAGCACTGCCCAAAGTTCCTGACGCATTCCAACGAGAAGATTGTGAGGGTAATCTTGCCATGCAAAAAACAGATAGTTGAGGCAGAGGGTGTGCAGGCGAGGATGGTGATGACGGCGAAGGAGCAGCTCTTAGATCAATTCTTGATTAAGGAGTACTTCAACAATAGCTTGCAGACACATCAACTCTGCATATTGGAATTGCAAGACTTCATCAACTCGGCTCGTGAGGTCCATTCATGCATTACAGATGTGGTACTTGAGAGGGAAGTGTCGAAGAAGCTCAAGGAGTCTGAGAAGAAGTATCACGATTCTGCGGAAGCAGTTGTTGCCAAGGTGAAATCTCTTTTCGACCAACATATAGACATGCTTGACAAGTTGAAAGATCGAATTCAGAAGTTTGATAAGCAGCTGGAGCTTACTCGTACTTTAAAGAAGGTCGTAAATGTGGTGTTCATGGGTATGTTGTCTGGTCTGTTTGTTTGCACCATCATGGCGGCGGCTATGGCTGCTCCGCCAGTTGCGTCAGCCTTGACCTGTCATCCATGTATTGCCGGAGTTATTGCTGGTGGTGCCGTTGGTTATGCTTTTTCGTCAGTATTAAAAGAGGGACAACACTGGCTTCTGACTTTGATTCAGGGTTATGAATCAACTCTGGAGGCACGCACGGGTGTAATTAAGTCGATGGAAGCCGGAACCAGAGACGCCATTAAAGAGTTGGATGACATCATGTGTCATGTTGATAAGGTCATTGCAAAAGGGAATCCAGTGTTGTCCAATTTAAGCTCTGCAACTGATGAGGTAAGAGTGGATGTGGTTGATCAGGAAGTGACGTCTATTCTGGAAAAGCTGGCAGAGTTTGGCCAGGAAATTGAGGAACTGGACGAGAAAAGAGAGCAGTGCCTCCGGGTTATAGAGACCGCAAGGAATAAGGTTCTAGGGGAATATGGCGAATTGAAGAGTAGCCCAGTGTGGAACACGCAGATCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

410

Amino Acids

45.83

Weight (kDa)

6.13

Isoelectric Point (pI)

34.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 67 - 392 7.8e-28 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1070
AccB1I GGYRCC 2 cut(s) 197, 806
AciI CCGC 7 cut(s) 143, 214, 551, 731, 734, 749, 1155
AcoI YGGCCR 1 cut(s) 1096
AcsI RAATTY 2 cut(s) 111, 624
AcyI GRCGYC 4 cut(s) 180, 198, 939, 1067
AfaI GTAC 4 cut(s) 220, 392, 492, 661
AgsI TTSAA 6 cut(s) 23, 119, 238, 397, 616, 1192
AjnI CCWGG 1 cut(s) 1098
AloI GAACNNNNNNTCC 1 cut(s) 35
AluBI AGCT 8 cut(s) 192, 364, 405, 517, 646, 652, 1020, 1084
AluI AGCT 8 cut(s) 192, 364, 405, 517, 646, 652, 1020, 1084
Alw26I GTCTC 2 cut(s) 930, 1145
AoxI GGCC 1 cut(s) 1096
ApeKI GCWGC 3 cut(s) 361, 643, 743
ApoI RAATTY 2 cut(s) 111, 624
ArsI GACNNNNNNTTYG 2 cut(s) 195, 227
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 466
AsuC2I CCSGG 1 cut(s) 1142
AsuHPI GGTGA 2 cut(s) 355, 583
AvaII GGWCC 1 cut(s) 466
BalI TGGCCA 1 cut(s) 1098
BanI GGYRCC 2 cut(s) 197, 806
BauI CACGAG 1 cut(s) 459
BbvI GCAGC 3 cut(s) 373, 655, 730
BccI CCATC 4 cut(s) 101, 334, 731, 913
BceAI ACGGC 2 cut(s) 366, 794
BciT130I CCWGG 1 cut(s) 1100
BclI TGATCA 1 cut(s) 1054
BcnI CCSGG 1 cut(s) 1142
BcoDI GTCTC 2 cut(s) 930, 1145
BfaI CTAG 1 cut(s) 1172
BfoI RGCGCY 1 cut(s) 201
BisI GCNGC 6 cut(s) 144, 362, 644, 732, 735, 744
BlsI GCNGC 6 cut(s) 145, 363, 645, 733, 736, 745
BmcAI AGTACT 1 cut(s) 392
Bme1390I CCNGG 2 cut(s) 1100, 1142
Bme18I GGWCC 1 cut(s) 466
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 4 cut(s) 13, 199, 808, 931
BmrFI CCNGG 2 cut(s) 1100, 1142
BmrI ACTGGG 1 cut(s) 1196
BmuI ACTGGG 1 cut(s) 1196
BpmI CTGGAG 2 cut(s) 668, 911
BpuEI CTTGAG 2 cut(s) 503, 515
BpuMI CCSGG 1 cut(s) 1142
BsaHI GRCGYC 4 cut(s) 180, 198, 939, 1067
BsaI GGTCTC 1 cut(s) 1145
BsaJI CCNNGG 1 cut(s) 567
Bse1I ACTGG 6 cut(s) 15, 752, 857, 1001, 1119, 1202
Bse3DI GCAATG 2 cut(s) 13, 984
BseBI CCWGG 1 cut(s) 1100
BseDI CCNNGG 1 cut(s) 567
BseGI GGATG 5 cut(s) 62, 345, 775, 961, 1051
BseMI GCAATG 2 cut(s) 13, 984
BseMII CTCAG 2 cut(s) 202, 519
BseNI ACTGG 6 cut(s) 15, 752, 857, 1001, 1119, 1202
BseXI GCAGC 3 cut(s) 373, 655, 730
BsgI GTGCAG 1 cut(s) 350
BshFI GGCC 1 cut(s) 1098
BshNI GGYRCC 2 cut(s) 197, 806
BsiSI CCGG 4 cut(s) 195, 789, 927, 1141
BslFI GGGAC 1 cut(s) 858
BsmAI GTCTC 2 cut(s) 930, 1145
BsmBI CGTCTC 1 cut(s) 930
BsmFI GGGAC 1 cut(s) 858
BsmI GAATGC 1 cut(s) 262
BsnI GGCC 1 cut(s) 1098
Bso31I GGTCTC 1 cut(s) 1145
Bsp143I GATC 6 cut(s) 131, 229, 370, 619, 1054, 1218
BspACI CCGC 7 cut(s) 143, 214, 551, 731, 734, 749, 1155
BspANI GGCC 1 cut(s) 1098
BspCNI CTCAG 2 cut(s) 201, 520
BspLI GGNNCC 4 cut(s) 13, 199, 808, 931
BspT107I GGYRCC 2 cut(s) 197, 806
BspTNI GGTCTC 1 cut(s) 1145
BsrDI GCAATG 2 cut(s) 13, 984
BsrI ACTGG 6 cut(s) 15, 752, 857, 1001, 1119, 1202
BssECI CCNNGG 1 cut(s) 567
BssMI GATC 6 cut(s) 131, 229, 370, 619, 1054, 1218
BssNI GRCGYC 4 cut(s) 180, 198, 939, 1067
BssSI CACGAG 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 459
Bst2UI CCWGG 1 cut(s) 1100
Bst6I CTCTTC 2 cut(s) 114, 1187
BstACI GRCGYC 4 cut(s) 180, 198, 939, 1067
BstAPI GCANNNNNTGC 1 cut(s) 563
BstC8I GCNNGC 5 cut(s) 148, 333, 407, 898, 1086
BstDEI CTNAG 4 cut(s) 72, 188, 367, 528
BstF5I GGATG 5 cut(s) 62, 345, 775, 961, 1051
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 6 cut(s) 134, 232, 373, 622, 1057, 1221
BstMAI GTCTC 2 cut(s) 930, 1145
BstMBI GATC 6 cut(s) 131, 229, 370, 619, 1054, 1218
BstMWI GCNNNNNNNGC 5 cut(s) 563, 649, 740, 743, 806
BstNI CCWGG 1 cut(s) 1100
BstNSI RCATGY 1 cut(s) 604
BstSCI CCNGG 2 cut(s) 1098, 1140
BstV1I GCAGC 3 cut(s) 373, 655, 730
BsuRI GGCC 1 cut(s) 1098
BtsCI GGATG 5 cut(s) 62, 345, 775, 961, 1051
BtsI GCAGTG 2 cut(s) 241, 1139
BtsIMutI CAGTG 6 cut(s) 22, 241, 850, 1008, 1139, 1209
Cac8I GCNNGC 5 cut(s) 148, 333, 407, 898, 1086
CfoI GCGC 1 cut(s) 200
Cfr13I GGNCC 1 cut(s) 466
CseI GACGC 4 cut(s) 188, 268, 747, 947
Csp6I GTAC 4 cut(s) 219, 391, 491, 660
CviAII CATG 8 cut(s) 297, 474, 601, 691, 727, 780, 964, 971
CviQI GTAC 4 cut(s) 219, 391, 491, 660
DdeI CTNAG 4 cut(s) 72, 188, 367, 528
DinI GGCGCC 1 cut(s) 199
DpnI GATC 6 cut(s) 133, 231, 372, 621, 1056, 1220
DpnII GATC 6 cut(s) 131, 229, 370, 619, 1054, 1218
DraI TTTAAA 1 cut(s) 666
EaeI YGGCCR 1 cut(s) 1096
Eam1104I CTCTTC 2 cut(s) 114, 1187
EarI CTCTTC 2 cut(s) 114, 1187
EciI GGCGGA 1 cut(s) 738
Eco130I CCWWGG 1 cut(s) 567
Eco31I GGTCTC 1 cut(s) 1145
Eco47I GGWCC 1 cut(s) 466
EcoRI GAATTC 1 cut(s) 624
EcoRII CCWGG 1 cut(s) 1098
EcoT14I CCWWGG 1 cut(s) 567
EcoT22I ATGCAT 1 cut(s) 479
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 567
Esp3I CGTCTC 1 cut(s) 930
FaeI CATG 8 cut(s) 300, 477, 604, 694, 730, 783, 967, 974
FalI AAGNNNNNCTT 4 cut(s) 50, 82, 377, 409
FaqI GGGAC 1 cut(s) 858
FatI CATG 8 cut(s) 296, 473, 600, 690, 726, 779, 963, 970
FbaI TGATCA 1 cut(s) 1054
Fnu4HI GCNGC 6 cut(s) 144, 362, 644, 732, 735, 744
FokI GGATG 5 cut(s) 49, 352, 762, 968, 1058
Fsp4HI GCNGC 6 cut(s) 144, 362, 644, 732, 735, 744
FspBI CTAG 1 cut(s) 1172
GlaI GCGC 1 cut(s) 199
GluI GCNGC 6 cut(s) 144, 362, 644, 732, 735, 744
GsuI CTGGAG 2 cut(s) 668, 911
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 1 cut(s) 1098
HapII CCGG 4 cut(s) 195, 789, 927, 1141
HgaI GACGC 4 cut(s) 188, 268, 747, 947
HhaI GCGC 1 cut(s) 200
Hin1I GRCGYC 4 cut(s) 180, 198, 939, 1067
Hin1II CATG 8 cut(s) 300, 477, 604, 694, 730, 783, 967, 974
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HinfI GANTC 6 cut(s) 76, 524, 545, 868, 881, 997
HpaII CCGG 4 cut(s) 195, 789, 927, 1141
HphI GGTGA 2 cut(s) 355, 583
Hpy188I TCNGA 4 cut(s) 103, 529, 630, 861
Hpy188III TCNNGA 9 cut(s) 227, 256, 379, 461, 542, 890, 1058, 1076, 1222
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 2 cut(s) 349, 664
HpyCH4IV ACGT 1 cut(s) 1067
HpyF10VI GCNNNNNNNGC 5 cut(s) 563, 649, 740, 743, 806
HpyF3I CTNAG 4 cut(s) 72, 188, 367, 528
HpySE526I ACGT 1 cut(s) 1067
Hsp92I GRCGYC 4 cut(s) 180, 198, 939, 1067
Hsp92II CATG 8 cut(s) 300, 477, 604, 694, 730, 783, 967, 974
HspAI GCGC 1 cut(s) 198
KasI GGCGCC 1 cut(s) 197
Ksp22I TGATCA 1 cut(s) 1054
Kzo9I GATC 6 cut(s) 131, 229, 370, 619, 1054, 1218
LmnI GCTCC 5 cut(s) 52, 197, 358, 649, 751
Lsp1109I GCAGC 3 cut(s) 373, 655, 730
MaeI CTAG 1 cut(s) 1172
MaeII ACGT 1 cut(s) 1067
MaeIII GTNAC 1 cut(s) 1063
MalI GATC 6 cut(s) 133, 231, 372, 621, 1056, 1220
MboI GATC 6 cut(s) 131, 229, 370, 619, 1054, 1218
MboII GAAGA 6 cut(s) 131, 215, 286, 523, 544, 1204
MlsI TGGCCA 1 cut(s) 1098
MluCI AATT 8 cut(s) 111, 374, 434, 624, 909, 1012, 1104, 1187
MluNI TGGCCA 1 cut(s) 1098
Mly113I GGCGCC 1 cut(s) 198
MlyI GAGTC 1 cut(s) 533
MmeI TCCRAC 2 cut(s) 291, 933
Mox20I TGGCCA 1 cut(s) 1098
Mph1103I ATGCAT 1 cut(s) 479
MscI TGGCCA 1 cut(s) 1098
MseI TTAA 6 cut(s) 384, 665, 836, 912, 945, 1016
MslI CAYNNNNRTG 2 cut(s) 695, 725
Msp20I TGGCCA 1 cut(s) 1098
MspA1I CMGCKG 1 cut(s) 646
MspI CCGG 4 cut(s) 195, 789, 927, 1141
MspR9I CCNGG 2 cut(s) 1100, 1142
Mva1269I GAATGC 1 cut(s) 262
MvaI CCWGG 1 cut(s) 1100
MwoI GCNNNNNNNGC 5 cut(s) 563, 649, 740, 743, 806
NarI GGCGCC 1 cut(s) 198
NciI CCSGG 1 cut(s) 1142
NdeII GATC 6 cut(s) 131, 229, 370, 619, 1054, 1218
NlaIII CATG 8 cut(s) 300, 477, 604, 694, 730, 783, 967, 974
NlaIV GGNNCC 4 cut(s) 13, 199, 808, 931
NmeAIII GCCGAG 1 cut(s) 434
NmuCI GTSAC 1 cut(s) 1063
NsiI ATGCAT 1 cut(s) 479
NspI RCATGY 1 cut(s) 604
PctI GAATGC 1 cut(s) 262
PfeI GAWTC 5 cut(s) 76, 545, 868, 881, 997
PkrI GCNGC 6 cut(s) 145, 363, 645, 733, 736, 745
PleI GAGTC 1 cut(s) 532
PluTI GGCGCC 1 cut(s) 201
PpsI GAGTC 1 cut(s) 532
Psp6I CCWGG 1 cut(s) 1098
PspGI CCWGG 1 cut(s) 1098
PspN4I GGNNCC 4 cut(s) 13, 199, 808, 931
PspPI GGNCC 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 646
RsaI GTAC 4 cut(s) 220, 392, 492, 661
RsaNI GTAC 4 cut(s) 219, 391, 491, 660
RseI CAYNNNNRTG 2 cut(s) 695, 725
SaqAI TTAA 6 cut(s) 384, 665, 836, 912, 945, 1016
SatI GCNGC 6 cut(s) 144, 362, 644, 732, 735, 744
Sau3AI GATC 6 cut(s) 131, 229, 370, 619, 1054, 1218
Sau96I GGNCC 1 cut(s) 466
ScaI AGTACT 1 cut(s) 392
SchI GAGTC 1 cut(s) 533
ScrFI CCNGG 2 cut(s) 1100, 1142
SfoI GGCGCC 1 cut(s) 199
SinI GGWCC 1 cut(s) 466
SmiMI CAYNNNNRTG 2 cut(s) 695, 725
SmlI CTYRAG 2 cut(s) 494, 518
SmoI CTYRAG 2 cut(s) 494, 518
Sse9I AATT 8 cut(s) 111, 374, 434, 624, 909, 1012, 1104, 1187
SsiI CCGC 7 cut(s) 143, 214, 551, 731, 734, 749, 1155
SspDI GGCGCC 1 cut(s) 197
SspMI CTAG 1 cut(s) 1172
StyD4I CCNGG 2 cut(s) 1098, 1140
StyI CCWWGG 1 cut(s) 567
TaiI ACGT 1 cut(s) 1070
TaqI TCGA 5 cut(s) 177, 509, 585, 622, 917
TasI AATT 8 cut(s) 111, 374, 434, 624, 909, 1012, 1104, 1187
TatI WGTACW 2 cut(s) 218, 390
TauI GCSGC 3 cut(s) 146, 734, 737
TfiI GAWTC 5 cut(s) 76, 545, 868, 881, 997
Tru1I TTAA 6 cut(s) 384, 665, 836, 912, 945, 1016
Tru9I TTAA 6 cut(s) 384, 665, 836, 912, 945, 1016
TscAI CASTG 6 cut(s) 22, 248, 857, 1008, 1139, 1209
TseFI GTSAC 1 cut(s) 1063
TseI GCWGC 3 cut(s) 361, 643, 743
Tsp45I GTSAC 1 cut(s) 1063
TspDTI ATGAA 4 cut(s) 436, 462, 679, 894
TspRI CASTG 6 cut(s) 22, 248, 857, 1008, 1139, 1209
VpaK11BI GGWCC 1 cut(s) 466
XapI RAATTY 2 cut(s) 111, 624
XceI RCATGY 1 cut(s) 604
XcmI CCANNNNNNNNNTGG 1 cut(s) 949
XspI CTAG 1 cut(s) 1172
ZraI GACGTC 1 cut(s) 1068
ZrmI AGTACT 1 cut(s) 392
Zsp2I ATGCAT 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.