Rroxscaffold_7G00205080

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010523
Physical Location & Seq
Reverse (-)
54605481 .. 54606713
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00205080.1

Sequence Viewer

Length: 1233 bp
ATGGGCAATGGAACCAGTGTTCAAGCAAAGCCAGAGAAACGACCCGTTTTGGAGCGAAAGACATCCTTTGCTAAGAATCATAGGATTGTTCCCATCAATTCCGAGCCAAGAATTTTTGAAGAGTTGATGAGATCGTGTCAGGTGGCTTGCAAAGATGACAAAGAGTTGCAAGACTTCGACGCCGAACTCAGCTCCGGCGCCGAAGAAGTCGTCCGCAGTACCGGTCTTGATCGGTTCAAGCAATGCCTAAAGTACCTGACGCAATCCAACGAGAAGATTGTGAGGGTAATCTTGTCATGCAAAAAACAGATAGTTGAGGCAGAGGGTGTGCAGGCGAGGATGGTGATGACGGCGAATGAGCAGCTCTTGGATCAATTTTTGATCAAGGAGTACTTCAACAATAGCTTGCAGACACTTCAACTCTGCAGATTGGAATTGCAAGACTTTATCAACTCGGCTCGTGAGGTCCATTCATGCATTACAGATGTGGTACTTGATTGGGAAGTGTCGAAGAAGCTCAACGAGTCTCAGAAGATGTATCACCGTTCTGCGGAAGCAGTTGTTGCCAAGGTGAACTCTCTGCGCGACCACCATAGAGACATGCTTGACAAGTTGAAAGATCGAATTCAGAAGTTTGATAAGAAGCTGGAGCTTACTCGTACTTTAAAGAAGGTCGTAAATGTGGTGTTCATGGGTATGTTGTCTGGTCTGTTTGTTTGCACCATCATGGCGGCGGCTATGGCTGCTCCGCCAGTTGCGTCAGCCTTGACCTCTCATCCATGTATTGCCGGAGTTATTGCTGGTGGTGCCGTTGGTTATGCTTTTTCGTCAGTATTAAAGGAGGGACAACACTGGCTTCTGACTTTGATTCAAGGTTATGAATCAACTCTGGATGCACATACGGGTGTAATTAAGTCGATGGAAGCCGGAACCAGAGACGCCATTAAAGAGTTGGATGACATCATGTGTCATGTTAATAAGGTCATTGCAAAAGGGACTGATCCAGTGTTGTCCAATTTAAGCTCCGCAAATGATGAGGGAAGAGTGGATGTGGTTGATCAGGAAGTGACGTCTATTCTGGAAAAGCTGGCAGAGTTTGGCGAGGAAATTGAGGATCTGGACGAGAAAAGAGCGCAGTGCCTCCGGGTTATAGAGACTGCAAGGGATAAGGTTCTAGGGGAATATGGCGAATTGAAGAGTAGCCCAGTGTGGAACACGAAGATCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

410

Amino Acids

45.9

Weight (kDa)

6.2

Isoelectric Point (pI)

35.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 67 - 393 3.6e-28 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1073
AccB1I GGYRCC 2 cut(s) 197, 806
AccII CGCG 1 cut(s) 585
AciI CCGC 6 cut(s) 214, 551, 731, 734, 749, 1026
AclWI GGATC 3 cut(s) 378, 995, 1122
AcsI RAATTY 2 cut(s) 111, 624
AcyI GRCGYC 4 cut(s) 180, 198, 939, 1070
AfaI GTAC 5 cut(s) 220, 254, 392, 492, 661
AfiI CCNNNNNNNGG 1 cut(s) 550
AgeI ACCGGT 1 cut(s) 221
AgsI TTSAA 8 cut(s) 23, 119, 238, 397, 419, 616, 872, 1195
AloI GAACNNNNNNTCC 1 cut(s) 35
AluBI AGCT 8 cut(s) 192, 364, 405, 517, 646, 652, 1023, 1087
AluI AGCT 8 cut(s) 192, 364, 405, 517, 646, 652, 1023, 1087
Alw26I GTCTC 4 cut(s) 531, 591, 930, 1148
AlwI GGATC 3 cut(s) 378, 995, 1122
ApeKI GCWGC 2 cut(s) 361, 743
ApoI RAATTY 2 cut(s) 111, 624
ArsI GACNNNNNNTTYG 2 cut(s) 195, 227
AsiGI ACCGGT 1 cut(s) 221
AspLEI GCGC 3 cut(s) 200, 585, 1135
AspS9I GGNCC 1 cut(s) 466
AsuC2I CCSGG 1 cut(s) 1145
AsuHPI GGTGA 3 cut(s) 355, 533, 583
AvaII GGWCC 1 cut(s) 466
BanI GGYRCC 2 cut(s) 197, 806
BauI CACGAG 1 cut(s) 459
BbvI GCAGC 2 cut(s) 373, 730
BccI CCATC 4 cut(s) 101, 334, 731, 913
BceAI ACGGC 2 cut(s) 366, 794
BclI TGATCA 2 cut(s) 381, 1057
BcnI CCSGG 1 cut(s) 1145
BcoDI GTCTC 4 cut(s) 531, 591, 930, 1148
BfaI CTAG 1 cut(s) 1175
BfmI CTRYAG 1 cut(s) 424
BfoI RGCGCY 1 cut(s) 201
BisI GCNGC 4 cut(s) 362, 732, 735, 744
BlsI GCNGC 4 cut(s) 363, 733, 736, 745
BmcAI AGTACT 1 cut(s) 392
Bme1390I CCNGG 1 cut(s) 1145
Bme18I GGWCC 1 cut(s) 466
BmgT120I GGNCC 1 cut(s) 466
BmiI GGNNCC 4 cut(s) 13, 199, 808, 931
BmrFI CCNGG 1 cut(s) 1145
BmrI ACTGGG 1 cut(s) 1199
BmsI GCATC 1 cut(s) 883
BmuI ACTGGG 1 cut(s) 1199
BpmI CTGGAG 1 cut(s) 668
BpuMI CCSGG 1 cut(s) 1145
BsaHI GRCGYC 4 cut(s) 180, 198, 939, 1070
BsaJI CCNNGG 1 cut(s) 567
BsaWI WCCGGW 1 cut(s) 221
Bsc4I CCNNNNNNNGG 1 cut(s) 550
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 5 cut(s) 15, 752, 857, 1004, 1205
Bse3DI GCAATG 3 cut(s) 13, 248, 984
BseDI CCNNGG 1 cut(s) 567
BseGI GGATG 6 cut(s) 62, 345, 775, 898, 961, 1054
BseLI CCNNNNNNNGG 1 cut(s) 550
BseMI GCAATG 3 cut(s) 13, 248, 984
BseMII CTCAG 2 cut(s) 202, 542
BseNI ACTGG 5 cut(s) 15, 752, 857, 1004, 1205
BseXI GCAGC 2 cut(s) 373, 730
BsgI GTGCAG 1 cut(s) 350
Bsh1236I CGCG 1 cut(s) 585
BshNI GGYRCC 2 cut(s) 197, 806
BshTI ACCGGT 1 cut(s) 221
BsiSI CCGG 5 cut(s) 195, 222, 789, 927, 1144
BslFI GGGAC 2 cut(s) 858, 1009
BslI CCNNNNNNNGG 1 cut(s) 550
BsmAI GTCTC 4 cut(s) 531, 591, 930, 1148
BsmBI CGTCTC 1 cut(s) 930
BsmFI GGGAC 2 cut(s) 858, 1009
Bsp143I GATC 9 cut(s) 131, 229, 370, 381, 619, 1000, 1057, 1114, 1221
BspACI CCGC 6 cut(s) 214, 551, 731, 734, 749, 1026
BspCNI CTCAG 2 cut(s) 201, 541
BspFNI CGCG 1 cut(s) 585
BspLI GGNNCC 4 cut(s) 13, 199, 808, 931
BspMAI CTGCAG 1 cut(s) 428
BspPI GGATC 3 cut(s) 378, 995, 1122
BspT107I GGYRCC 2 cut(s) 197, 806
BsrDI GCAATG 3 cut(s) 13, 248, 984
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 5 cut(s) 15, 752, 857, 1004, 1205
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 1 cut(s) 567
BssMI GATC 9 cut(s) 131, 229, 370, 381, 619, 1000, 1057, 1114, 1221
BssNI GRCGYC 4 cut(s) 180, 198, 939, 1070
BssSI CACGAG 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 459
Bst4CI ACNGT 1 cut(s) 545
Bst6I CTCTTC 3 cut(s) 114, 1036, 1190
BstACI GRCGYC 4 cut(s) 180, 198, 939, 1070
BstAPI GCANNNNNTGC 1 cut(s) 563
BstC8I GCNNGC 4 cut(s) 148, 333, 407, 1089
BstDEI CTNAG 3 cut(s) 72, 188, 528
BstF5I GGATG 6 cut(s) 62, 345, 775, 898, 961, 1054
BstFNI CGCG 1 cut(s) 585
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 3 cut(s) 200, 585, 1135
BstKTI GATC 9 cut(s) 134, 232, 373, 384, 622, 1003, 1060, 1117, 1224
BstMAI GTCTC 4 cut(s) 531, 591, 930, 1148
BstMBI GATC 9 cut(s) 131, 229, 370, 381, 619, 1000, 1057, 1114, 1221
BstMWI GCNNNNNNNGC 4 cut(s) 563, 740, 743, 806
BstNSI RCATGY 1 cut(s) 604
BstSCI CCNGG 1 cut(s) 1143
BstSFI CTRYAG 1 cut(s) 424
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 2 cut(s) 373, 730
BstX2I RGATCY 1 cut(s) 1114
BstYI RGATCY 1 cut(s) 1114
BtsCI GGATG 6 cut(s) 62, 345, 775, 898, 961, 1054
BtsI GCAGTG 1 cut(s) 1142
BtsIMutI CAGTG 5 cut(s) 22, 850, 1011, 1142, 1212
Cac8I GCNNGC 4 cut(s) 148, 333, 407, 1089
CfoI GCGC 3 cut(s) 200, 585, 1135
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 1 cut(s) 466
CseI GACGC 4 cut(s) 188, 268, 747, 947
Csp6I GTAC 5 cut(s) 219, 253, 391, 491, 660
CspAI ACCGGT 1 cut(s) 221
CviAII CATG 8 cut(s) 297, 474, 601, 691, 727, 780, 964, 971
CviQI GTAC 5 cut(s) 219, 253, 391, 491, 660
DdeI CTNAG 3 cut(s) 72, 188, 528
DinI GGCGCC 1 cut(s) 199
DpnI GATC 9 cut(s) 133, 231, 372, 383, 621, 1002, 1059, 1116, 1223
DpnII GATC 9 cut(s) 131, 229, 370, 381, 619, 1000, 1057, 1114, 1221
DraI TTTAAA 1 cut(s) 666
Eam1104I CTCTTC 3 cut(s) 114, 1036, 1190
EarI CTCTTC 3 cut(s) 114, 1036, 1190
EciI GGCGGA 1 cut(s) 738
Eco130I CCWWGG 1 cut(s) 567
Eco47I GGWCC 1 cut(s) 466
EcoRI GAATTC 1 cut(s) 624
EcoT14I CCWWGG 1 cut(s) 567
EcoT22I ATGCAT 1 cut(s) 479
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 567
Esp3I CGTCTC 1 cut(s) 930
FaeI CATG 8 cut(s) 300, 477, 604, 694, 730, 783, 967, 974
FalI AAGNNNNNCTT 4 cut(s) 50, 82, 377, 409
FaqI GGGAC 2 cut(s) 858, 1009
FatI CATG 8 cut(s) 296, 473, 600, 690, 726, 779, 963, 970
FbaI TGATCA 2 cut(s) 381, 1057
Fnu4HI GCNGC 4 cut(s) 362, 732, 735, 744
FokI GGATG 6 cut(s) 49, 352, 762, 905, 968, 1061
Fsp4HI GCNGC 4 cut(s) 362, 732, 735, 744
FspBI CTAG 1 cut(s) 1175
GlaI GCGC 3 cut(s) 199, 584, 1134
GluI GCNGC 4 cut(s) 362, 732, 735, 744
GsuI CTGGAG 1 cut(s) 668
HaeII RGCGCY 1 cut(s) 201
HapII CCGG 5 cut(s) 195, 222, 789, 927, 1144
HgaI GACGC 4 cut(s) 188, 268, 747, 947
HhaI GCGC 3 cut(s) 200, 585, 1135
Hin1I GRCGYC 4 cut(s) 180, 198, 939, 1070
Hin1II CATG 8 cut(s) 300, 477, 604, 694, 730, 783, 967, 974
Hin6I GCGC 3 cut(s) 198, 583, 1133
HinP1I GCGC 3 cut(s) 198, 583, 1133
HinfI GANTC 4 cut(s) 76, 524, 868, 881
HpaII CCGG 5 cut(s) 195, 222, 789, 927, 1144
HphI GGTGA 3 cut(s) 355, 533, 583
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 4 cut(s) 103, 531, 630, 861
Hpy188III TCNNGA 7 cut(s) 227, 461, 890, 1061, 1079, 1118, 1225
Hpy8I GTNNAC 1 cut(s) 574
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 1 cut(s) 664
HpyCH4III ACNGT 1 cut(s) 545
HpyCH4IV ACGT 1 cut(s) 1070
HpyF10VI GCNNNNNNNGC 4 cut(s) 563, 740, 743, 806
HpyF3I CTNAG 3 cut(s) 72, 188, 528
HpySE526I ACGT 1 cut(s) 1070
Hsp92I GRCGYC 4 cut(s) 180, 198, 939, 1070
Hsp92II CATG 8 cut(s) 300, 477, 604, 694, 730, 783, 967, 974
HspAI GCGC 3 cut(s) 198, 583, 1133
KasI GGCGCC 1 cut(s) 197
Ksp22I TGATCA 2 cut(s) 381, 1057
Kzo9I GATC 9 cut(s) 131, 229, 370, 381, 619, 1000, 1057, 1114, 1221
LmnI GCTCC 5 cut(s) 52, 197, 649, 751, 1028
Lsp1109I GCAGC 2 cut(s) 373, 730
LweI GCATC 1 cut(s) 883
MaeI CTAG 1 cut(s) 1175
MaeII ACGT 1 cut(s) 1070
MaeIII GTNAC 1 cut(s) 1066
MalI GATC 9 cut(s) 133, 231, 372, 383, 621, 1002, 1059, 1116, 1223
MboI GATC 9 cut(s) 131, 229, 370, 381, 619, 1000, 1057, 1114, 1221
MboII GAAGA 8 cut(s) 131, 215, 286, 523, 544, 1053, 1207, 1231
MflI RGATCY 1 cut(s) 1114
MluCI AATT 9 cut(s) 97, 111, 374, 434, 624, 909, 1015, 1107, 1190
Mly113I GGCGCC 1 cut(s) 198
MlyI GAGTC 1 cut(s) 533
MmeI TCCRAC 2 cut(s) 291, 933
Mph1103I ATGCAT 1 cut(s) 479
MseI TTAA 6 cut(s) 665, 836, 912, 945, 975, 1019
MslI CAYNNNNRTG 3 cut(s) 695, 725, 903
MspI CCGG 5 cut(s) 195, 222, 789, 927, 1144
MspR9I CCNGG 1 cut(s) 1145
MvnI CGCG 1 cut(s) 585
MwoI GCNNNNNNNGC 4 cut(s) 563, 740, 743, 806
NarI GGCGCC 1 cut(s) 198
NciI CCSGG 1 cut(s) 1145
NdeII GATC 9 cut(s) 131, 229, 370, 381, 619, 1000, 1057, 1114, 1221
NlaIII CATG 8 cut(s) 300, 477, 604, 694, 730, 783, 967, 974
NlaIV GGNNCC 4 cut(s) 13, 199, 808, 931
NmeAIII GCCGAG 1 cut(s) 434
NmuCI GTSAC 1 cut(s) 1066
NsiI ATGCAT 1 cut(s) 479
NspI RCATGY 1 cut(s) 604
PfeI GAWTC 3 cut(s) 76, 868, 881
PinAI ACCGGT 1 cut(s) 221
PkrI GCNGC 4 cut(s) 363, 733, 736, 745
PleI GAGTC 1 cut(s) 532
PluTI GGCGCC 1 cut(s) 201
PpsI GAGTC 1 cut(s) 532
PspN4I GGNNCC 4 cut(s) 13, 199, 808, 931
PspPI GGNCC 1 cut(s) 466
PstI CTGCAG 1 cut(s) 428
PsuI RGATCY 1 cut(s) 1114
RsaI GTAC 5 cut(s) 220, 254, 392, 492, 661
RsaNI GTAC 5 cut(s) 219, 253, 391, 491, 660
RseI CAYNNNNRTG 3 cut(s) 695, 725, 903
SaqAI TTAA 6 cut(s) 665, 836, 912, 945, 975, 1019
SatI GCNGC 4 cut(s) 362, 732, 735, 744
Sau3AI GATC 9 cut(s) 131, 229, 370, 381, 619, 1000, 1057, 1114, 1221
Sau96I GGNCC 1 cut(s) 466
ScaI AGTACT 1 cut(s) 392
SchI GAGTC 1 cut(s) 533
ScrFI CCNGG 1 cut(s) 1145
SfaNI GCATC 1 cut(s) 883
SfcI CTRYAG 1 cut(s) 424
SfoI GGCGCC 1 cut(s) 199
SinI GGWCC 1 cut(s) 466
SmiMI CAYNNNNRTG 3 cut(s) 695, 725, 903
Sse9I AATT 9 cut(s) 97, 111, 374, 434, 624, 909, 1015, 1107, 1190
SsiI CCGC 6 cut(s) 214, 551, 731, 734, 749, 1026
SspDI GGCGCC 1 cut(s) 197
SspMI CTAG 1 cut(s) 1175
StyD4I CCNGG 1 cut(s) 1143
StyI CCWWGG 1 cut(s) 567
TaaI ACNGT 1 cut(s) 545
TaiI ACGT 1 cut(s) 1073
TaqI TCGA 4 cut(s) 177, 509, 622, 917
TasI AATT 9 cut(s) 97, 111, 374, 434, 624, 909, 1015, 1107, 1190
TatI WGTACW 1 cut(s) 390
TauI GCSGC 2 cut(s) 734, 737
TfiI GAWTC 3 cut(s) 76, 868, 881
Tru1I TTAA 6 cut(s) 665, 836, 912, 945, 975, 1019
Tru9I TTAA 6 cut(s) 665, 836, 912, 945, 975, 1019
TscAI CASTG 5 cut(s) 22, 857, 1011, 1142, 1212
TseFI GTSAC 1 cut(s) 1066
TseI GCWGC 2 cut(s) 361, 743
Tsp45I GTSAC 1 cut(s) 1066
TspDTI ATGAA 3 cut(s) 462, 679, 894
TspRI CASTG 5 cut(s) 22, 857, 1011, 1142, 1212
VpaK11BI GGWCC 1 cut(s) 466
XapI RAATTY 2 cut(s) 111, 624
XceI RCATGY 1 cut(s) 604
XcmI CCANNNNNNNNNTGG 1 cut(s) 949
XspI CTAG 1 cut(s) 1175
ZraI GACGTC 1 cut(s) 1071
ZrmI AGTACT 1 cut(s) 392
Zsp2I ATGCAT 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.