RCHIOBHM_CHR0C38G0503041

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_chinensis
Unknown
OG0010523
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1224 bp
ATGGGCGGTGTTCAAGCAAAGCCAGAGAGACGACCCGTTTTGGAGCGAAAGACATCCTTTGCTAAGAATCATAGGATTGTTCCCATCAATTCCGAGCCAAGAATTTTTGAAGAGTTGATGAGATCGTATCAGGCGGATTGCAAAGATGACAAAGAGTTGCAAGACTTCGACGCCGAACTCAGCTCCGGCGCCGAAGAAGTCGTCCGCAGTACCGGTCTTGATCGGTTCAAGCAATGCCTAAAGTACCTGACGCATTCCAACGAGAAGATTGTGAGGGTAATCTTGTCATGCAAAAAACAGATAGTTGAGGCAGAGGGTGTGGGGGCGAGGATGGTGATGACGGCGAATGAGCAGCTCTTGGATCAATTCTTGATCAAGGAGTACTTCAACAATAGCTTGCAGACACTTCAACTCTGCAGATTGGAATTGCAGGACTTTATCAACTCGGCTCGTGAGGTCCATTCATGCATTACAGATGTGGTACTTGATTGGGAAGTGTCGAAGAAGCTCAACGAGTCTCAGAAGATGTATCACCGTTCTGCGGAAGCAGTTGTTGCCAAGGTGAAATCTCTGCGCGACCAACATAGAGACATGCTTGACAAGCTGAAAGATCGAATTGTGAAGTTTGATAGGAAGCTGGAGCTTACTCGTACTTTAAAGAAGGTCGTAAATGTGGTGTTCATGGGTATGTTGTCTGGTCTGTTTGTTTGCACCATCATGGCGGCGGCTATGGCTGCTCCGCCAGTTGCGTCGGCCATGACCTCTCATCCATGTATCGCCGGAGTTGTTGCTGGTGGTGCCGTTGGTTATGCTTTTTCGGCAGTATTAAAAGAGGGACAACACTGGCTTCTGACTTTTATTCAAGGTTATAAATCAACTCTGGAGGCACATACGGGTGTAATTAAGTCGATGGAAGCCGGAACCCGAGACGCCATTAAAGAGTTGGATGACATCATGTGTCATGTTAATAAGGTCATTGCAAAAGGGAACGATCCAGTGTTGTCCAATTTAAGCTCCACAAATGATGAGGGAAGAGTGGATGTGGTTGATCAGGAAGTGACGTCTATTCTGGAAAAGCTGGCAGAGTTTGGCAAGGAAATTGAGGAACTGGACGAGAAAAGAGAGCAGTGCCTCCGGGTTATAGAGACTGCAAGGGATAAGGTTCTAGGGGAATATGGCGAATTGAAGAGTAGCCCAGTGTGGAACACGAAGATCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

407

Amino Acids

45.8

Weight (kDa)

6.78

Isoelectric Point (pI)

33.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 870
AatII GACGTC 1 cut(s) 1064
AccB1I GGYRCC 2 cut(s) 188, 797
AccII CGCG 1 cut(s) 576
AciI CCGC 7 cut(s) 6, 134, 205, 542, 722, 725, 740
AclWI GGATC 2 cut(s) 369, 986
AcoI YGGCCR 1 cut(s) 753
AcsI RAATTY 1 cut(s) 102
AcyI GRCGYC 4 cut(s) 171, 189, 930, 1061
AfaI GTAC 5 cut(s) 211, 245, 383, 483, 652
AfiI CCNNNNNNNGG 1 cut(s) 541
AgeI ACCGGT 1 cut(s) 212
AgsI TTSAA 7 cut(s) 14, 110, 229, 388, 410, 863, 1186
AluBI AGCT 9 cut(s) 183, 355, 396, 508, 604, 637, 643, 1014, 1078
AluI AGCT 9 cut(s) 183, 355, 396, 508, 604, 637, 643, 1014, 1078
Alw26I GTCTC 5 cut(s) 22, 522, 582, 921, 1139
AlwI GGATC 2 cut(s) 369, 986
Ama87I CYCGRG 1 cut(s) 924
AoxI GGCC 1 cut(s) 753
ApeKI GCWGC 2 cut(s) 352, 734
ApoI RAATTY 1 cut(s) 102
ArsI GACNNNNNNTTYG 2 cut(s) 186, 218
AsiGI ACCGGT 1 cut(s) 212
AspLEI GCGC 2 cut(s) 191, 576
AspS9I GGNCC 1 cut(s) 457
AsuC2I CCSGG 1 cut(s) 1136
AsuHPI GGTGA 3 cut(s) 346, 524, 574
AvaI CYCGRG 1 cut(s) 924
AvaII GGWCC 1 cut(s) 457
BanI GGYRCC 2 cut(s) 188, 797
BauI CACGAG 1 cut(s) 450
BbvI GCAGC 2 cut(s) 364, 721
BccI CCATC 4 cut(s) 92, 325, 722, 904
BceAI ACGGC 2 cut(s) 357, 785
BclI TGATCA 2 cut(s) 372, 1048
BcnI CCSGG 1 cut(s) 1136
BcoDI GTCTC 5 cut(s) 22, 522, 582, 921, 1139
BfaI CTAG 1 cut(s) 1166
BfmI CTRYAG 1 cut(s) 415
BfoI RGCGCY 1 cut(s) 192
BisI GCNGC 4 cut(s) 353, 723, 726, 735
BlsI GCNGC 4 cut(s) 354, 724, 727, 736
BmcAI AGTACT 1 cut(s) 383
Bme1390I CCNGG 1 cut(s) 1136
Bme18I GGWCC 1 cut(s) 457
BmeT110I CYCGRG 1 cut(s) 924
BmgT120I GGNCC 1 cut(s) 457
BmiI GGNNCC 3 cut(s) 190, 799, 922
BmrFI CCNGG 1 cut(s) 1136
BmrI ACTGGG 1 cut(s) 1190
BmuI ACTGGG 1 cut(s) 1190
BpmI CTGGAG 2 cut(s) 659, 902
BpuMI CCSGG 1 cut(s) 1136
BsaHI GRCGYC 4 cut(s) 171, 189, 930, 1061
BsaJI CCNNGG 1 cut(s) 558
BsaWI WCCGGW 1 cut(s) 212
Bsc4I CCNNNNNNNGG 1 cut(s) 541
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 5 cut(s) 743, 848, 995, 1113, 1196
Bse3DI GCAATG 2 cut(s) 239, 975
BseDI CCNNGG 1 cut(s) 558
BseGI GGATG 5 cut(s) 53, 336, 766, 952, 1045
BseLI CCNNNNNNNGG 1 cut(s) 541
BseMI GCAATG 2 cut(s) 239, 975
BseMII CTCAG 2 cut(s) 193, 533
BseNI ACTGG 5 cut(s) 743, 848, 995, 1113, 1196
BseXI GCAGC 2 cut(s) 364, 721
Bsh1236I CGCG 1 cut(s) 576
BshFI GGCC 1 cut(s) 755
BshNI GGYRCC 2 cut(s) 188, 797
BshTI ACCGGT 1 cut(s) 212
BsiHKCI CYCGRG 1 cut(s) 924
BsiSI CCGG 5 cut(s) 186, 213, 780, 918, 1135
BslFI GGGAC 1 cut(s) 849
BslI CCNNNNNNNGG 1 cut(s) 541
BsmAI GTCTC 5 cut(s) 22, 522, 582, 921, 1139
BsmBI CGTCTC 2 cut(s) 22, 921
BsmFI GGGAC 1 cut(s) 849
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 1 cut(s) 755
BsoBI CYCGRG 1 cut(s) 924
Bsp143I GATC 8 cut(s) 122, 220, 361, 372, 610, 991, 1048, 1212
BspACI CCGC 7 cut(s) 6, 134, 205, 542, 722, 725, 740
BspANI GGCC 1 cut(s) 755
BspCNI CTCAG 2 cut(s) 192, 532
BspFNI CGCG 1 cut(s) 576
BspLI GGNNCC 3 cut(s) 190, 799, 922
BspMAI CTGCAG 1 cut(s) 419
BspPI GGATC 2 cut(s) 369, 986
BspT107I GGYRCC 2 cut(s) 188, 797
BsrDI GCAATG 2 cut(s) 239, 975
BsrFI RCCGGY 1 cut(s) 212
BsrI ACTGG 5 cut(s) 743, 848, 995, 1113, 1196
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 558
BssMI GATC 8 cut(s) 122, 220, 361, 372, 610, 991, 1048, 1212
BssNI GRCGYC 4 cut(s) 171, 189, 930, 1061
BssSI CACGAG 1 cut(s) 450
BssT1I CCWWGG 1 cut(s) 558
Bst2BI CACGAG 1 cut(s) 450
Bst4CI ACNGT 1 cut(s) 536
Bst6I CTCTTC 3 cut(s) 105, 1027, 1181
BstACI GRCGYC 4 cut(s) 171, 189, 930, 1061
BstAPI GCANNNNNTGC 1 cut(s) 554
BstC8I GCNNGC 2 cut(s) 398, 1080
BstDEI CTNAG 3 cut(s) 63, 179, 519
BstF5I GGATG 5 cut(s) 53, 336, 766, 952, 1045
BstFNI CGCG 1 cut(s) 576
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 2 cut(s) 191, 576
BstKTI GATC 8 cut(s) 125, 223, 364, 375, 613, 994, 1051, 1215
BstMAI GTCTC 5 cut(s) 22, 522, 582, 921, 1139
BstMBI GATC 8 cut(s) 122, 220, 361, 372, 610, 991, 1048, 1212
BstMWI GCNNNNNNNGC 6 cut(s) 554, 601, 731, 734, 797, 818
BstNSI RCATGY 1 cut(s) 595
BstSCI CCNGG 1 cut(s) 1134
BstSFI CTRYAG 1 cut(s) 415
BstUI CGCG 1 cut(s) 576
BstV1I GCAGC 2 cut(s) 364, 721
BsuRI GGCC 1 cut(s) 755
BtsCI GGATG 5 cut(s) 53, 336, 766, 952, 1045
BtsI GCAGTG 1 cut(s) 1133
BtsIMutI CAGTG 4 cut(s) 841, 1002, 1133, 1203
Cac8I GCNNGC 2 cut(s) 398, 1080
CfoI GCGC 2 cut(s) 191, 576
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 457
CseI GACGC 4 cut(s) 179, 259, 738, 938
Csp6I GTAC 5 cut(s) 210, 244, 382, 482, 651
CspAI ACCGGT 1 cut(s) 212
CviAII CATG 9 cut(s) 288, 465, 592, 682, 718, 757, 771, 955, 962
CviQI GTAC 5 cut(s) 210, 244, 382, 482, 651
DdeI CTNAG 3 cut(s) 63, 179, 519
DinI GGCGCC 1 cut(s) 190
DpnI GATC 8 cut(s) 124, 222, 363, 374, 612, 993, 1050, 1214
DpnII GATC 8 cut(s) 122, 220, 361, 372, 610, 991, 1048, 1212
DraI TTTAAA 1 cut(s) 657
EaeI YGGCCR 1 cut(s) 753
Eam1104I CTCTTC 3 cut(s) 105, 1027, 1181
EarI CTCTTC 3 cut(s) 105, 1027, 1181
EciI GGCGGA 2 cut(s) 149, 729
Eco130I CCWWGG 1 cut(s) 558
Eco47I GGWCC 1 cut(s) 457
Eco88I CYCGRG 1 cut(s) 924
EcoT14I CCWWGG 1 cut(s) 558
EcoT22I ATGCAT 1 cut(s) 470
EgeI GGCGCC 1 cut(s) 190
EheI GGCGCC 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 558
Esp3I CGTCTC 2 cut(s) 22, 921
FaeI CATG 9 cut(s) 291, 468, 595, 685, 721, 760, 774, 958, 965
FalI AAGNNNNNCTT 4 cut(s) 41, 73, 368, 400
FaqI GGGAC 1 cut(s) 849
FatI CATG 9 cut(s) 287, 464, 591, 681, 717, 756, 770, 954, 961
FbaI TGATCA 2 cut(s) 372, 1048
Fnu4HI GCNGC 4 cut(s) 353, 723, 726, 735
FokI GGATG 5 cut(s) 40, 343, 753, 959, 1052
Fsp4HI GCNGC 4 cut(s) 353, 723, 726, 735
FspBI CTAG 1 cut(s) 1166
GlaI GCGC 2 cut(s) 190, 575
GluI GCNGC 4 cut(s) 353, 723, 726, 735
GsuI CTGGAG 2 cut(s) 659, 902
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 1 cut(s) 755
HapII CCGG 5 cut(s) 186, 213, 780, 918, 1135
HgaI GACGC 4 cut(s) 179, 259, 738, 938
HhaI GCGC 2 cut(s) 191, 576
Hin1I GRCGYC 4 cut(s) 171, 189, 930, 1061
Hin1II CATG 9 cut(s) 291, 468, 595, 685, 721, 760, 774, 958, 965
Hin6I GCGC 2 cut(s) 189, 574
HinP1I GCGC 2 cut(s) 189, 574
HinfI GANTC 2 cut(s) 67, 515
HpaII CCGG 5 cut(s) 186, 213, 780, 918, 1135
HphI GGTGA 3 cut(s) 346, 524, 574
Hpy188I TCNGA 3 cut(s) 94, 522, 852
Hpy188III TCNNGA 7 cut(s) 218, 370, 452, 881, 1052, 1070, 1216
Hpy99I CGWCG 2 cut(s) 173, 754
HpyAV CCTTC 1 cut(s) 655
HpyCH4III ACNGT 1 cut(s) 536
HpyCH4IV ACGT 1 cut(s) 1061
HpyF10VI GCNNNNNNNGC 6 cut(s) 554, 601, 731, 734, 797, 818
HpyF3I CTNAG 3 cut(s) 63, 179, 519
HpySE526I ACGT 1 cut(s) 1061
Hsp92I GRCGYC 4 cut(s) 171, 189, 930, 1061
Hsp92II CATG 9 cut(s) 291, 468, 595, 685, 721, 760, 774, 958, 965
HspAI GCGC 2 cut(s) 189, 574
KasI GGCGCC 1 cut(s) 188
Ksp22I TGATCA 2 cut(s) 372, 1048
Kzo9I GATC 8 cut(s) 122, 220, 361, 372, 610, 991, 1048, 1212
LmnI GCTCC 5 cut(s) 43, 188, 640, 742, 1019
Lsp1109I GCAGC 2 cut(s) 364, 721
MaeI CTAG 1 cut(s) 1166
MaeII ACGT 1 cut(s) 1061
MaeIII GTNAC 1 cut(s) 1057
MalI GATC 8 cut(s) 124, 222, 363, 374, 612, 993, 1050, 1214
MboI GATC 8 cut(s) 122, 220, 361, 372, 610, 991, 1048, 1212
MboII GAAGA 8 cut(s) 122, 206, 277, 514, 535, 1044, 1198, 1222
MluCI AATT 9 cut(s) 88, 102, 365, 425, 615, 900, 1006, 1098, 1181
Mly113I GGCGCC 1 cut(s) 189
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 2 cut(s) 282, 924
Mph1103I ATGCAT 1 cut(s) 470
MseI TTAA 6 cut(s) 656, 827, 903, 936, 966, 1010
MslI CAYNNNNRTG 3 cut(s) 686, 716, 894
MspI CCGG 5 cut(s) 186, 213, 780, 918, 1135
MspR9I CCNGG 1 cut(s) 1136
Mva1269I GAATGC 1 cut(s) 253
MvnI CGCG 1 cut(s) 576
MwoI GCNNNNNNNGC 6 cut(s) 554, 601, 731, 734, 797, 818
NarI GGCGCC 1 cut(s) 189
NciI CCSGG 1 cut(s) 1136
NdeII GATC 8 cut(s) 122, 220, 361, 372, 610, 991, 1048, 1212
NlaIII CATG 9 cut(s) 291, 468, 595, 685, 721, 760, 774, 958, 965
NlaIV GGNNCC 3 cut(s) 190, 799, 922
NmeAIII GCCGAG 1 cut(s) 425
NmuCI GTSAC 1 cut(s) 1057
NsiI ATGCAT 1 cut(s) 470
NspI RCATGY 1 cut(s) 595
PctI GAATGC 1 cut(s) 253
PfeI GAWTC 1 cut(s) 67
PinAI ACCGGT 1 cut(s) 212
PkrI GCNGC 4 cut(s) 354, 724, 727, 736
PleI GAGTC 1 cut(s) 523
PluTI GGCGCC 1 cut(s) 192
PpsI GAGTC 1 cut(s) 523
PsiI TTATAA 1 cut(s) 870
PspN4I GGNNCC 3 cut(s) 190, 799, 922
PspPI GGNCC 1 cut(s) 457
PstI CTGCAG 1 cut(s) 419
RsaI GTAC 5 cut(s) 211, 245, 383, 483, 652
RsaNI GTAC 5 cut(s) 210, 244, 382, 482, 651
RseI CAYNNNNRTG 3 cut(s) 686, 716, 894
SaqAI TTAA 6 cut(s) 656, 827, 903, 936, 966, 1010
SatI GCNGC 4 cut(s) 353, 723, 726, 735
Sau3AI GATC 8 cut(s) 122, 220, 361, 372, 610, 991, 1048, 1212
Sau96I GGNCC 1 cut(s) 457
ScaI AGTACT 1 cut(s) 383
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 1 cut(s) 1136
SfcI CTRYAG 1 cut(s) 415
SfoI GGCGCC 1 cut(s) 190
SinI GGWCC 1 cut(s) 457
SmiMI CAYNNNNRTG 3 cut(s) 686, 716, 894
Sse9I AATT 9 cut(s) 88, 102, 365, 425, 615, 900, 1006, 1098, 1181
SsiI CCGC 7 cut(s) 6, 134, 205, 542, 722, 725, 740
SspDI GGCGCC 1 cut(s) 188
SspMI CTAG 1 cut(s) 1166
StyD4I CCNGG 1 cut(s) 1134
StyI CCWWGG 1 cut(s) 558
TaaI ACNGT 1 cut(s) 536
TaiI ACGT 1 cut(s) 1064
TaqI TCGA 4 cut(s) 168, 500, 613, 908
TasI AATT 9 cut(s) 88, 102, 365, 425, 615, 900, 1006, 1098, 1181
TatI WGTACW 1 cut(s) 381
TauI GCSGC 2 cut(s) 725, 728
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 6 cut(s) 656, 827, 903, 936, 966, 1010
Tru9I TTAA 6 cut(s) 656, 827, 903, 936, 966, 1010
TscAI CASTG 4 cut(s) 848, 1002, 1133, 1203
TseFI GTSAC 1 cut(s) 1057
TseI GCWGC 2 cut(s) 352, 734
Tsp45I GTSAC 1 cut(s) 1057
TspDTI ATGAA 2 cut(s) 453, 670
TspRI CASTG 4 cut(s) 848, 1002, 1133, 1203
VpaK11BI GGWCC 1 cut(s) 457
XapI RAATTY 1 cut(s) 102
XceI RCATGY 1 cut(s) 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 940
XspI CTAG 1 cut(s) 1166
ZraI GACGTC 1 cut(s) 1062
ZrmI AGTACT 1 cut(s) 383
Zsp2I ATGCAT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.