Rroxscaffold_7G00205120

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010523
Physical Location & Seq
Reverse (-)
54652655 .. 54653911
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00205120.1

Sequence Viewer

Length: 1257 bp
ATGGGCGGTGTTCAAGCAAAGCCAGAGAGACGACGCCCTGGCCCTGCTTTGGAGCGGAAGACATCTTTTGCTAATAAGAAGCATAGGGTTGTTCCCATCAATGCCGAACCGAGGCCAACTGACGACCTCGGCAAGCATTTTGAAGACTTGATGAGATCGTATCAGGCAGATTGCAAAGATGACAAAGACTTGCAAGACTTCGACGCCGAACTCAGATCCAGCGCCAAAGAAGTCATCTGCAGTACCGGTCTTGATCGGTTTAAGCAATGCCTAAAGTATCTGACGCATTCCAACGAGAAGATGGTGAGGGTAATCTTGTCATGCAAAAAACAGATACTTGAGGCAGAGGGTGTGCAGGCGAGGATGGTGATGATGGCGAATGAGAAGCTCTTAGATCAATTCTTGATCAAGGAGTACATCAACAATAGCTTGCAGACGCTTCAACTCTGCAGATTGGAATTGCAAGACTTCATCAAGTCGGCTCGTAAGGTCCATTCATGCATTGCAGATGTGGTACTTGAGAGGAAAGTGTCGAAGAAGCTCAAGGAGTCTGAGAAGAAGTATCACGATTCTGCGGAAGCAGTTGTTGACAAGGTGAAATCTCTGCGCGCCCAACATAAAGACATGCTTGACAAGTTGAAAGATCGAATTCAGAAGTTTGATAAGAAGCTGGAGCTTACTCGTACTTTAAAAAGGGTCGTAAATGTGGTGTTCTTAGGTATGTTGTCTGGTCTGTTTGTTTGCACCATCATGGCTGCGGCTATGGCTGCTCCGCCAGTTGCGTCGGCCTTGACCTCTCATCCATGTATTGCCGGAGTTATTGCTGGTGGTGCCGTTTGTTATGCTTTTTCGTCAGTATTAAAAGAGGGACAACACTGGCTTCTGACTTTGATTCAAGGTTATGAATCAACTCTGGAGGCACATACGGGTGTAATTAAGTCGATGGAAGCCGGAACCGGAGAAGCCATTAAAGAGTTGGATGACATCATGTGTCATGTTAATAAGGTCATTGCAAAAGGGAATGATCCAGTGTTGACCAATTTAAGCTCTGCAAGTGATGAGGGAAGAGTGGATGTGGTTGATCAGGAAGTGACGTCTATTCTGGAGAAGCTGACAGAGTTTGGCAAGGAAATTGAGGAACTGGACGAGAGAAGAAAGCAGTGCCTCCGGGTTATAGAGACGGCAAGGGATAAGGTTGTGGGGGAATATGACGAATTGAAGACCAGCCGAGTGTGGAACACGGAGATCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

418

Amino Acids

47.05

Weight (kDa)

8.48

Isoelectric Point (pI)

36.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 79 - 400 4e-24 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1097
AccB1I GGYRCC 1 cut(s) 830
AccBSI CCGCTC 1 cut(s) 55
AccII CGCG 1 cut(s) 609
AciI CCGC 5 cut(s) 6, 55, 575, 758, 773
AclWI GGATC 2 cut(s) 210, 1019
AcsI RAATTY 1 cut(s) 648
AcyI GRCGYC 3 cut(s) 34, 204, 1094
AfaI GTAC 4 cut(s) 244, 416, 516, 685
AfiI CCNNNNNNNGG 2 cut(s) 49, 111
AgeI ACCGGT 1 cut(s) 245
AgsI TTSAA 6 cut(s) 14, 143, 443, 640, 896, 1219
AjnI CCWGG 1 cut(s) 37
AluBI AGCT 7 cut(s) 388, 429, 541, 670, 676, 1047, 1111
AluI AGCT 7 cut(s) 388, 429, 541, 670, 676, 1047, 1111
Alw26I GTCTC 2 cut(s) 22, 1172
AlwI GGATC 2 cut(s) 210, 1019
AoxI GGCC 3 cut(s) 40, 113, 786
ApeKI GCWGC 2 cut(s) 755, 767
ApoI RAATTY 1 cut(s) 648
AsiGI ACCGGT 1 cut(s) 245
AspLEI GCGC 3 cut(s) 224, 609, 611
AspS9I GGNCC 2 cut(s) 41, 490
AsuC2I CCSGG 1 cut(s) 1169
AsuHPI GGTGA 3 cut(s) 316, 379, 607
AvaII GGWCC 1 cut(s) 490
BanI GGYRCC 1 cut(s) 830
BbsI GAAGAC 3 cut(s) 65, 150, 1226
BbvI GCAGC 2 cut(s) 742, 754
BccI CCATC 6 cut(s) 104, 295, 358, 367, 755, 937
BceAI ACGGC 2 cut(s) 818, 1197
BciT130I CCWGG 1 cut(s) 39
BclI TGATCA 2 cut(s) 405, 1081
BcnI CCSGG 1 cut(s) 1169
BcoDI GTCTC 2 cut(s) 22, 1172
BfmI CTRYAG 2 cut(s) 238, 448
BfoI RGCGCY 1 cut(s) 225
BisI GCNGC 3 cut(s) 756, 759, 768
BlsI GCNGC 3 cut(s) 757, 760, 769
Bme1390I CCNGG 2 cut(s) 39, 1169
Bme18I GGWCC 1 cut(s) 490
BmgT120I GGNCC 2 cut(s) 41, 490
BmiI GGNNCC 2 cut(s) 832, 955
BmrFI CCNGG 2 cut(s) 39, 1169
BpiI GAAGAC 3 cut(s) 65, 150, 1226
BpmI CTGGAG 3 cut(s) 692, 935, 1124
BpuEI CTTGAG 3 cut(s) 359, 527, 539
BpuMI CCSGG 1 cut(s) 1169
BsaHI GRCGYC 3 cut(s) 34, 204, 1094
BsaJI CCNNGG 3 cut(s) 37, 110, 127
BsaWI WCCGGW 2 cut(s) 245, 956
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 111
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 4 cut(s) 776, 881, 1028, 1146
Bse3DI GCAATG 3 cut(s) 272, 501, 1008
BseBI CCWGG 1 cut(s) 39
BseDI CCNNGG 3 cut(s) 37, 110, 127
BseGI GGATG 4 cut(s) 369, 799, 985, 1078
BseLI CCNNNNNNNGG 2 cut(s) 49, 111
BseMI GCAATG 3 cut(s) 272, 501, 1008
BseMII CTCAG 2 cut(s) 226, 543
BseNI ACTGG 4 cut(s) 776, 881, 1028, 1146
BsePI GCGCGC 1 cut(s) 607
BseXI GCAGC 2 cut(s) 742, 754
BsgI GTGCAG 1 cut(s) 374
Bsh1236I CGCG 1 cut(s) 609
BshFI GGCC 3 cut(s) 42, 115, 788
BshNI GGYRCC 1 cut(s) 830
BshTI ACCGGT 1 cut(s) 245
BsiSI CCGG 5 cut(s) 246, 813, 951, 957, 1168
BslFI GGGAC 1 cut(s) 882
BslI CCNNNNNNNGG 2 cut(s) 49, 111
BsmAI GTCTC 2 cut(s) 22, 1172
BsmBI CGTCTC 2 cut(s) 22, 1172
BsmFI GGGAC 1 cut(s) 882
BsmI GAATGC 1 cut(s) 286
BsnI GGCC 3 cut(s) 42, 115, 788
Bsp143I GATC 9 cut(s) 155, 215, 253, 394, 405, 643, 1024, 1081, 1245
BspACI CCGC 5 cut(s) 6, 55, 575, 758, 773
BspANI GGCC 3 cut(s) 42, 115, 788
BspCNI CTCAG 2 cut(s) 225, 544
BspFNI CGCG 1 cut(s) 609
BspLI GGNNCC 2 cut(s) 832, 955
BspMAI CTGCAG 2 cut(s) 242, 452
BspPI GGATC 2 cut(s) 210, 1019
BspT107I GGYRCC 1 cut(s) 830
BsrBI CCGCTC 1 cut(s) 55
BsrDI GCAATG 3 cut(s) 272, 501, 1008
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 4 cut(s) 776, 881, 1028, 1146
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 3 cut(s) 37, 110, 127
BssHII GCGCGC 1 cut(s) 607
BssMI GATC 9 cut(s) 155, 215, 253, 394, 405, 643, 1024, 1081, 1245
BssNI GRCGYC 3 cut(s) 34, 204, 1094
Bst2UI CCWGG 1 cut(s) 39
Bst6I CTCTTC 1 cut(s) 1060
BstACI GRCGYC 3 cut(s) 34, 204, 1094
BstC8I GCNNGC 4 cut(s) 134, 357, 431, 609
BstDEI CTNAG 4 cut(s) 212, 391, 552, 715
BstF5I GGATG 4 cut(s) 369, 799, 985, 1078
BstFNI CGCG 1 cut(s) 609
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 3 cut(s) 224, 609, 611
BstKTI GATC 9 cut(s) 158, 218, 256, 397, 408, 646, 1027, 1084, 1248
BstMAI GTCTC 2 cut(s) 22, 1172
BstMBI GATC 9 cut(s) 155, 215, 253, 394, 405, 643, 1024, 1081, 1245
BstMWI GCNNNNNNNGC 3 cut(s) 764, 767, 830
BstNI CCWGG 1 cut(s) 39
BstNSI RCATGY 1 cut(s) 628
BstSCI CCNGG 2 cut(s) 37, 1167
BstSFI CTRYAG 2 cut(s) 238, 448
BstUI CGCG 1 cut(s) 609
BstV1I GCAGC 2 cut(s) 742, 754
BstV2I GAAGAC 3 cut(s) 65, 150, 1226
BstX2I RGATCY 1 cut(s) 215
BstYI RGATCY 1 cut(s) 215
BsuRI GGCC 3 cut(s) 42, 115, 788
BtsCI GGATG 4 cut(s) 369, 799, 985, 1078
BtsI GCAGTG 1 cut(s) 1166
BtsIMutI CAGTG 3 cut(s) 874, 1035, 1166
Cac8I GCNNGC 4 cut(s) 134, 357, 431, 609
CfoI GCGC 3 cut(s) 224, 609, 611
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 2 cut(s) 41, 490
CseI GACGC 5 cut(s) 42, 212, 292, 445, 771
Csp6I GTAC 4 cut(s) 243, 415, 515, 684
CspAI ACCGGT 1 cut(s) 245
CviAII CATG 7 cut(s) 321, 498, 625, 751, 804, 988, 995
CviQI GTAC 4 cut(s) 243, 415, 515, 684
DdeI CTNAG 4 cut(s) 212, 391, 552, 715
DpnI GATC 9 cut(s) 157, 217, 255, 396, 407, 645, 1026, 1083, 1247
DpnII GATC 9 cut(s) 155, 215, 253, 394, 405, 643, 1024, 1081, 1245
DraI TTTAAA 1 cut(s) 690
Eam1104I CTCTTC 1 cut(s) 1060
EarI CTCTTC 1 cut(s) 1060
EciI GGCGGA 1 cut(s) 762
Eco47I GGWCC 1 cut(s) 490
EcoRI GAATTC 1 cut(s) 648
EcoRII CCWGG 1 cut(s) 37
EcoT22I ATGCAT 1 cut(s) 503
Esp3I CGTCTC 2 cut(s) 22, 1172
FaeI CATG 7 cut(s) 324, 501, 628, 754, 807, 991, 998
FalI AAGNNNNNCTT 2 cut(s) 612, 644
FaqI GGGAC 1 cut(s) 882
FatI CATG 7 cut(s) 320, 497, 624, 750, 803, 987, 994
FbaI TGATCA 2 cut(s) 405, 1081
Fnu4HI GCNGC 3 cut(s) 756, 759, 768
FokI GGATG 4 cut(s) 376, 786, 992, 1085
Fsp4HI GCNGC 3 cut(s) 756, 759, 768
GlaI GCGC 3 cut(s) 223, 608, 610
GluI GCNGC 3 cut(s) 756, 759, 768
GsuI CTGGAG 3 cut(s) 692, 935, 1124
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 3 cut(s) 42, 115, 788
HapII CCGG 5 cut(s) 246, 813, 951, 957, 1168
HgaI GACGC 5 cut(s) 42, 212, 292, 445, 771
HhaI GCGC 3 cut(s) 224, 609, 611
Hin1I GRCGYC 3 cut(s) 34, 204, 1094
Hin1II CATG 7 cut(s) 324, 501, 628, 754, 807, 991, 998
Hin6I GCGC 3 cut(s) 222, 607, 609
HinP1I GCGC 3 cut(s) 222, 607, 609
HincII GTYRAC 2 cut(s) 589, 1035
HindII GTYRAC 2 cut(s) 589, 1035
HinfI GANTC 4 cut(s) 548, 569, 892, 905
HpaII CCGG 5 cut(s) 246, 813, 951, 957, 1168
HphI GGTGA 3 cut(s) 316, 379, 607
Hpy166II GTNNAC 2 cut(s) 589, 1035
Hpy188I TCNGA 5 cut(s) 215, 282, 553, 654, 885
Hpy188III TCNNGA 7 cut(s) 251, 403, 566, 914, 1085, 1103, 1249
Hpy8I GTNNAC 2 cut(s) 589, 1035
Hpy99I CGWCG 3 cut(s) 36, 206, 787
HpyCH4IV ACGT 1 cut(s) 1094
HpyF10VI GCNNNNNNNGC 3 cut(s) 764, 767, 830
HpyF3I CTNAG 4 cut(s) 212, 391, 552, 715
HpySE526I ACGT 1 cut(s) 1094
Hsp92I GRCGYC 3 cut(s) 34, 204, 1094
Hsp92II CATG 7 cut(s) 324, 501, 628, 754, 807, 991, 998
HspAI GCGC 3 cut(s) 222, 607, 609
Ksp22I TGATCA 2 cut(s) 405, 1081
Kzo9I GATC 9 cut(s) 155, 215, 253, 394, 405, 643, 1024, 1081, 1245
LmnI GCTCC 3 cut(s) 52, 673, 775
Lsp1109I GCAGC 2 cut(s) 742, 754
MaeII ACGT 1 cut(s) 1094
MaeIII GTNAC 1 cut(s) 1090
MalI GATC 9 cut(s) 157, 217, 255, 396, 407, 645, 1026, 1083, 1247
MbiI CCGCTC 1 cut(s) 55
MboI GATC 9 cut(s) 155, 215, 253, 394, 405, 643, 1024, 1081, 1245
MboII GAAGA 8 cut(s) 70, 155, 310, 547, 568, 1077, 1164, 1231
MflI RGATCY 1 cut(s) 215
MluCI AATT 7 cut(s) 398, 458, 648, 933, 1039, 1131, 1214
MlyI GAGTC 1 cut(s) 557
MmeI TCCRAC 2 cut(s) 315, 957
Mph1103I ATGCAT 1 cut(s) 503
MseI TTAA 7 cut(s) 261, 689, 860, 936, 969, 999, 1043
MslI CAYNNNNRTG 2 cut(s) 749, 927
MspI CCGG 5 cut(s) 246, 813, 951, 957, 1168
MspR9I CCNGG 2 cut(s) 39, 1169
Mva1269I GAATGC 1 cut(s) 286
MvaI CCWGG 1 cut(s) 39
MvnI CGCG 1 cut(s) 609
MwoI GCNNNNNNNGC 3 cut(s) 764, 767, 830
NciI CCSGG 1 cut(s) 1169
NdeII GATC 9 cut(s) 155, 215, 253, 394, 405, 643, 1024, 1081, 1245
NlaIII CATG 7 cut(s) 324, 501, 628, 754, 807, 991, 998
NlaIV GGNNCC 2 cut(s) 832, 955
NmeAIII GCCGAG 2 cut(s) 108, 1253
NmuCI GTSAC 1 cut(s) 1090
NsiI ATGCAT 1 cut(s) 503
NspI RCATGY 1 cut(s) 628
PauI GCGCGC 1 cut(s) 607
PctI GAATGC 1 cut(s) 286
PfeI GAWTC 3 cut(s) 569, 892, 905
PinAI ACCGGT 1 cut(s) 245
PkrI GCNGC 3 cut(s) 757, 760, 769
PleI GAGTC 1 cut(s) 556
PpsI GAGTC 1 cut(s) 556
Psp6I CCWGG 1 cut(s) 37
PspGI CCWGG 1 cut(s) 37
PspN4I GGNNCC 2 cut(s) 832, 955
PspPI GGNCC 2 cut(s) 41, 490
PstI CTGCAG 2 cut(s) 242, 452
PsuI RGATCY 1 cut(s) 215
PteI GCGCGC 1 cut(s) 607
RsaI GTAC 4 cut(s) 244, 416, 516, 685
RsaNI GTAC 4 cut(s) 243, 415, 515, 684
RseI CAYNNNNRTG 2 cut(s) 749, 927
SaqAI TTAA 7 cut(s) 261, 689, 860, 936, 969, 999, 1043
SatI GCNGC 3 cut(s) 756, 759, 768
Sau3AI GATC 9 cut(s) 155, 215, 253, 394, 405, 643, 1024, 1081, 1245
Sau96I GGNCC 2 cut(s) 41, 490
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 2 cut(s) 39, 1169
SfcI CTRYAG 2 cut(s) 238, 448
SinI GGWCC 1 cut(s) 490
SmiMI CAYNNNNRTG 2 cut(s) 749, 927
SmlI CTYRAG 3 cut(s) 338, 518, 542
SmoI CTYRAG 3 cut(s) 338, 518, 542
Sse9I AATT 7 cut(s) 398, 458, 648, 933, 1039, 1131, 1214
SsiI CCGC 5 cut(s) 6, 55, 575, 758, 773
StyD4I CCNGG 2 cut(s) 37, 1167
TaiI ACGT 1 cut(s) 1097
TaqI TCGA 4 cut(s) 201, 533, 646, 941
TasI AATT 7 cut(s) 398, 458, 648, 933, 1039, 1131, 1214
TatI WGTACW 1 cut(s) 414
TauI GCSGC 1 cut(s) 761
TfiI GAWTC 3 cut(s) 569, 892, 905
Tru1I TTAA 7 cut(s) 261, 689, 860, 936, 969, 999, 1043
Tru9I TTAA 7 cut(s) 261, 689, 860, 936, 969, 999, 1043
TscAI CASTG 3 cut(s) 881, 1035, 1166
TseFI GTSAC 1 cut(s) 1090
TseI GCWGC 2 cut(s) 755, 767
Tsp45I GTSAC 1 cut(s) 1090
TspDTI ATGAA 3 cut(s) 460, 486, 918
TspGWI ACGGA 1 cut(s) 1256
TspRI CASTG 3 cut(s) 881, 1035, 1166
VpaK11BI GGWCC 1 cut(s) 490
XapI RAATTY 1 cut(s) 648
XceI RCATGY 1 cut(s) 628
XcmI CCANNNNNNNNNTGG 2 cut(s) 298, 973
ZraI GACGTC 1 cut(s) 1095
Zsp2I ATGCAT 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.