FvH4_2g11950

membrane protein At4g09580-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008356
Physical Location & Seq
Reverse (-)
10441924 .. 10449283
7360 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g11950.t1

Sequence Viewer

Length: 834 bp
ATGATGGCGGCTCCGAGGAATCTGGTGGTGAGGGACGAGGAGAATGCCAAGGTGGTTGTGGAGGAGGAGGAGGATTGGTCCACCGGCAAGAAGCCAAAGTCTTCAGAGAGACTGCCCCTCACGCGCTGGGAGGTTGCCGCCGCTCTCTCCGTATTCTTTATATTCTCCGCTGGCCTCTTCTGTGTCTACTTGACCATGCCCCCCGCCGATTACGGCAAACTCAAGCTTCCTCGCACCATTGCTGATCTTCGCTTGCTCAAAGATCATCTCGGGACTTATGCTAAGGATTATCCAGCTCAATTCATTCTGGGTTACTGTTCTACGTACATCTTCATGCAGACTTTCATGATTCCCGGAACCATCTTCATGTCATTGCTTGCCGGAGCTCTTTTCGGTGTTGTCAGAGGCCTTATGTTAGTTGTCTTCAACGCCACTGCCGGAGCTTCTTCCTGCTTCTTTTTGTCCAAGTTAATTGGCAGGCCCTTAGTTAGTTGGTTGTGGCCCGAAAAACTGAGGTTCTTTCAGGCAGAGATTGCAAAGCGGAAGGAGAAGCTGCTGAACTATATGCTGTTTCTGAGGATAACTCCAACATTGCCAAACCTTTTCATCAACTTGGCATCTCCCATTGTTGACATACCCTTTCACATTTTCTTCGTTGCAACTTTGGTTGGTCTTATTCCGGCATCTTATATCACAGTCAGAGCTGGCCTTGCTCTTGGGGATCTCAAGTCAGTCAAGGACCTCTACGATTTTAAAACATTATTTGTGCTGTTCCTTATTGGTTCAGTGATTATATTTCCAACTCTTCTGAAGAGAAAGCGGATCTATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

278

Amino Acids

31.32

Weight (kDa)

9.5

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 117 - 236 2.4e-15 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 186
AccII CGCG 1 cut(s) 124
AciI CCGC 7 cut(s) 8, 138, 141, 168, 204, 541, 820
AclWI GGATC 2 cut(s) 729, 830
AcuI CTGAAG 2 cut(s) 87, 830
AfaI GTAC 1 cut(s) 326
AgsI TTSAA 1 cut(s) 427
AluBI AGCT 6 cut(s) 226, 296, 386, 443, 553, 704
AluI AGCT 6 cut(s) 226, 296, 386, 443, 553, 704
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 1 cut(s) 103
AlwI GGATC 2 cut(s) 729, 830
Ama87I CYCGRG 1 cut(s) 269
AoxI GGCC 5 cut(s) 172, 406, 479, 500, 706
ApeKI GCWGC 1 cut(s) 553
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 4 cut(s) 78, 480, 501, 739
AsuC2I CCSGG 1 cut(s) 354
AsuHPI GGTGA 1 cut(s) 40
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 2 cut(s) 78, 739
BanII GRGCYC 1 cut(s) 388
BbsI GAAGAC 2 cut(s) 93, 415
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 540
BccI CCATC 1 cut(s) 368
BceAI ACGGC 1 cut(s) 229
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BcnI CCSGG 1 cut(s) 354
BcoDI GTCTC 1 cut(s) 103
BisI GCNGC 4 cut(s) 9, 138, 141, 554
BlsI GCNGC 4 cut(s) 10, 139, 142, 555
Bme1390I CCNGG 1 cut(s) 354
Bme18I GGWCC 2 cut(s) 78, 739
BmeT110I CYCGRG 1 cut(s) 269
BmgT120I GGNCC 4 cut(s) 78, 480, 501, 739
BmiI GGNNCC 2 cut(s) 12, 358
BmrFI CCNGG 1 cut(s) 354
BmsI GCATC 2 cut(s) 626, 692
BpiI GAAGAC 2 cut(s) 93, 415
BplI GAGNNNNNCTC 2 cut(s) 568, 600
Bpu10I CCTNAGC 1 cut(s) 282
BpuEI CTTGAG 2 cut(s) 206, 710
BpuMI CCSGG 1 cut(s) 354
BsaAI YACGTR 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 14, 48
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bse118I RCCGGY 1 cut(s) 83
Bse3DI GCAATG 3 cut(s) 237, 371, 590
BseDI CCNNGG 2 cut(s) 14, 48
BseMI GCAATG 3 cut(s) 237, 371, 590
BseMII CTCAG 2 cut(s) 503, 566
BseRI GAGGAG 4 cut(s) 53, 77, 80, 83
BseXI GCAGC 1 cut(s) 540
BseYI CCCAGC 1 cut(s) 126
Bsh1236I CGCG 1 cut(s) 124
BshFI GGCC 5 cut(s) 174, 408, 481, 502, 708
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 5 cut(s) 84, 354, 381, 438, 680
BslFI GGGAC 2 cut(s) 47, 286
BsmAI GTCTC 1 cut(s) 103
BsmFI GGGAC 2 cut(s) 47, 286
BsmI GAATGC 1 cut(s) 49
BsnI GGCC 5 cut(s) 174, 408, 481, 502, 708
BsoBI CYCGRG 1 cut(s) 269
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 4 cut(s) 244, 262, 721, 822
BspACI CCGC 7 cut(s) 8, 138, 141, 168, 204, 541, 820
BspANI GGCC 5 cut(s) 174, 408, 481, 502, 708
BspCNI CTCAG 2 cut(s) 504, 567
BspFNI CGCG 1 cut(s) 124
BspHI TCATGA 1 cut(s) 345
BspLI GGNNCC 2 cut(s) 12, 358
BspPI GGATC 2 cut(s) 729, 830
BsrBI CCGCTC 1 cut(s) 143
BsrDI GCAATG 3 cut(s) 237, 371, 590
BsrFI RCCGGY 1 cut(s) 83
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 14, 48
BssMI GATC 4 cut(s) 244, 262, 721, 822
BssT1I CCWWGG 1 cut(s) 48
Bst4CI ACNGT 2 cut(s) 317, 697
Bst6I CTCTTC 3 cut(s) 182, 806, 810
BstAPI GCANNNNNTGC 1 cut(s) 533
BstBAI YACGTR 1 cut(s) 324
BstC8I GCNNGC 5 cut(s) 172, 254, 378, 479, 706
BstDEI CTNAG 4 cut(s) 282, 484, 512, 575
BstFNI CGCG 1 cut(s) 124
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 4 cut(s) 247, 265, 724, 825
BstMAI GTCTC 1 cut(s) 103
BstMBI GATC 4 cut(s) 244, 262, 721, 822
BstMWI GCNNNNNNNGC 3 cut(s) 121, 533, 710
BstSCI CCNGG 1 cut(s) 352
BstSNI TACGTA 1 cut(s) 324
BstUI CGCG 1 cut(s) 124
BstV1I GCAGC 1 cut(s) 540
BstV2I GAAGAC 2 cut(s) 93, 415
BstX2I RGATCY 2 cut(s) 721, 822
BstYI RGATCY 2 cut(s) 721, 822
BsuRI GGCC 5 cut(s) 174, 408, 481, 502, 708
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 2 cut(s) 432, 792
Cac8I GCNNGC 5 cut(s) 172, 254, 378, 479, 706
CciI TCATGA 1 cut(s) 345
CfoI GCGC 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 4 cut(s) 78, 480, 501, 739
Csp6I GTAC 1 cut(s) 325
CviAII CATG 4 cut(s) 196, 334, 346, 367
CviQI GTAC 1 cut(s) 325
DdeI CTNAG 4 cut(s) 282, 484, 512, 575
DpnI GATC 4 cut(s) 246, 264, 723, 824
DpnII GATC 4 cut(s) 244, 262, 721, 822
DraI TTTAAA 1 cut(s) 754
Eam1104I CTCTTC 3 cut(s) 182, 806, 810
EarI CTCTTC 3 cut(s) 182, 806, 810
Ecl136II GAGCTC 1 cut(s) 386
Eco105I TACGTA 1 cut(s) 324
Eco130I CCWWGG 1 cut(s) 48
Eco147I AGGCCT 1 cut(s) 408
Eco24I GRGCYC 1 cut(s) 388
Eco47I GGWCC 2 cut(s) 78, 739
Eco53kI GAGCTC 1 cut(s) 386
Eco57I CTGAAG 2 cut(s) 87, 830
Eco88I CYCGRG 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 386
EcoO109I RGGNCCY 2 cut(s) 480, 739
EcoT14I CCWWGG 1 cut(s) 48
EcoT38I GRGCYC 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 4 cut(s) 199, 337, 349, 370
FaqI GGGAC 2 cut(s) 47, 286
FatI CATG 4 cut(s) 195, 333, 345, 366
FauI CCCGC 1 cut(s) 211
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 4 cut(s) 9, 138, 141, 554
FriOI GRGCYC 1 cut(s) 388
Fsp4HI GCNGC 4 cut(s) 9, 138, 141, 554
GlaI GCGC 1 cut(s) 125
GluI GCNGC 4 cut(s) 9, 138, 141, 554
GsaI CCCAGC 1 cut(s) 130
HaeIII GGCC 5 cut(s) 174, 408, 481, 502, 708
HapII CCGG 5 cut(s) 84, 354, 381, 438, 680
HhaI GCGC 1 cut(s) 126
Hin1II CATG 4 cut(s) 199, 337, 349, 370
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 2 cut(s) 19, 349
HpaII CCGG 5 cut(s) 84, 354, 381, 438, 680
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 3 cut(s) 81, 187, 631
Hpy188I TCNGA 6 cut(s) 15, 106, 404, 576, 701, 810
Hpy188III TCNNGA 2 cut(s) 271, 346
Hpy8I GTNNAC 3 cut(s) 81, 187, 631
HpyAV CCTTC 1 cut(s) 538
HpyCH4III ACNGT 2 cut(s) 317, 697
HpyCH4IV ACGT 1 cut(s) 323
HpyCH4V TGCA 3 cut(s) 337, 536, 659
HpyF10VI GCNNNNNNNGC 3 cut(s) 121, 533, 710
HpyF3I CTNAG 4 cut(s) 282, 484, 512, 575
HpySE526I ACGT 1 cut(s) 323
Hsp92II CATG 4 cut(s) 199, 337, 349, 370
HspAI GCGC 1 cut(s) 124
Kzo9I GATC 4 cut(s) 244, 262, 721, 822
LmnI GCTCC 3 cut(s) 16, 383, 440
Lsp1109I GCAGC 1 cut(s) 540
LweI GCATC 2 cut(s) 626, 692
MaeII ACGT 1 cut(s) 323
MaeIII GTNAC 1 cut(s) 311
MalI GATC 4 cut(s) 246, 264, 723, 824
MbiI CCGCTC 1 cut(s) 143
MboI GATC 4 cut(s) 244, 262, 721, 822
MflI RGATCY 2 cut(s) 721, 822
MhlI GDGCHC 1 cut(s) 388
MluCI AATT 2 cut(s) 299, 471
MmeI TCCRAC 2 cut(s) 611, 824
MseI TTAA 2 cut(s) 470, 753
MslI CAYNNNNRTG 2 cut(s) 332, 365
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 5 cut(s) 84, 354, 381, 438, 680
MspR9I CCNGG 1 cut(s) 354
Mva1269I GAATGC 1 cut(s) 49
MvnI CGCG 1 cut(s) 124
MwoI GCNNNNNNNGC 3 cut(s) 121, 533, 710
NciI CCSGG 1 cut(s) 354
NdeII GATC 4 cut(s) 244, 262, 721, 822
NlaIII CATG 4 cut(s) 199, 337, 349, 370
NlaIV GGNNCC 2 cut(s) 12, 358
PagI TCATGA 1 cut(s) 345
PceI AGGCCT 1 cut(s) 408
PctI GAATGC 1 cut(s) 49
PfeI GAWTC 2 cut(s) 19, 349
PfoI TCCNGGA 1 cut(s) 352
PkrI GCNGC 4 cut(s) 10, 139, 142, 555
Ppu21I YACGTR 1 cut(s) 324
PpuMI RGGWCCY 1 cut(s) 739
Psp124BI GAGCTC 1 cut(s) 388
Psp5II RGGWCCY 1 cut(s) 739
PspFI CCCAGC 1 cut(s) 126
PspN4I GGNNCC 2 cut(s) 12, 358
PspPI GGNCC 4 cut(s) 78, 480, 501, 739
PspPPI RGGWCCY 1 cut(s) 739
PsuI RGATCY 2 cut(s) 721, 822
RsaI GTAC 1 cut(s) 326
RsaNI GTAC 1 cut(s) 325
RseI CAYNNNNRTG 2 cut(s) 332, 365
SacI GAGCTC 1 cut(s) 388
SaqAI TTAA 2 cut(s) 470, 753
SatI GCNGC 4 cut(s) 9, 138, 141, 554
Sau3AI GATC 4 cut(s) 244, 262, 721, 822
Sau96I GGNCC 4 cut(s) 78, 480, 501, 739
ScrFI CCNGG 1 cut(s) 354
SduI GDGCHC 1 cut(s) 388
SfaNI GCATC 2 cut(s) 626, 692
SinI GGWCC 2 cut(s) 78, 739
SmiMI CAYNNNNRTG 2 cut(s) 332, 365
SmlI CTYRAG 2 cut(s) 221, 725
SmoI CTYRAG 2 cut(s) 221, 725
SnaBI TACGTA 1 cut(s) 324
Sse9I AATT 2 cut(s) 299, 471
SseBI AGGCCT 1 cut(s) 408
SsiI CCGC 7 cut(s) 8, 138, 141, 168, 204, 541, 820
SstI GAGCTC 1 cut(s) 388
StuI AGGCCT 1 cut(s) 408
StyD4I CCNGG 1 cut(s) 352
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 2 cut(s) 317, 697
TaiI ACGT 1 cut(s) 326
TasI AATT 2 cut(s) 299, 471
TauI GCSGC 3 cut(s) 11, 140, 143
TfiI GAWTC 2 cut(s) 19, 349
Tru1I TTAA 2 cut(s) 470, 753
Tru9I TTAA 2 cut(s) 470, 753
TscAI CASTG 2 cut(s) 439, 792
TseI GCWGC 1 cut(s) 553
TspDTI ATGAA 5 cut(s) 292, 322, 334, 355, 595
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 2 cut(s) 439, 792
VpaK11BI GGWCC 2 cut(s) 78, 739
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.