Rorug06G0064500

membrane protein At4g09580-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000153
Physical Location & Seq
Forward (+)
8593097 .. 8601092
7996 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0064500.1

Sequence Viewer

Length: 873 bp
ATGGAAGTAGAGGTCGACGAGAAGGAGCTCAAAGCCGCCGGAGCCGAGTTGTTCTCCGACGGACGCCGCGGCCTTCGAATCCACGGCTGGGAAATCGAGACTCGCAACCGCTCCATTCTCAACTCCTCCACCCACGAACTGTGGGAGAAAAAGCTTGAGACTTGTCACATGCCGGAGATGATATTTGGGGAGAGTTGTTTGGTTCTGAAGCATGTGAAGAGTGGCACCACTATTCACTTCAATGCTTTTGATGCTCTGATTGGGTGGAAGAAGGAGGCTTTGCCTCCAGTTGAAGTCCCAGCTGCTGCTCAGTGGAAGTTCAGATGTAAACCCTCTGAGCAAGTGATACTGGATTATGACTATACATTCACAACACCGTACGGAGGAAGTGAAACTATTACCTTGGATGCAGACAAGCACGAAGGAGGAGAGGTATCTAAGGAGACCTGCAATCTCCACTGGGAGGACTGCAAAGAAAAAATTGATGTGGTTGCATTGGCATCTAAAGAGCCTATTCTCTTTTATGATGAGGTAGTCTTGTATGAAGATGAATTGGCGGATAATGGTGTGTCTCTTCTAACTGTAAAAGTGAGAGTCATGCCGAGCGGTTGGTTTCTTCTCTTGCGTTTTTGGCTTAGAGTTGACGGAGTGCTTATGAGATTAAGGGATACCCGTATGCACTGTGTTTTTGGAGATAATGTAAATCCCATTATTCTTCGAGAGAGCTGCTGGAGAGAAGCTACATTTCAAGCTTTGGCTGCAAAGGGATACCCTTCTGAAGCTGCTGCCTACAATGATCCAAGTATCATCAGCCAAAGGCTTCCTGTGGTCATGAGCAAGACCCAAAAGCTAAAGGTATCTAGTAACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

290

Amino Acids

32.98

Weight (kDa)

5.48

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIP41 PF04176 60 - 252 1.4e-56 TIP41-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000153)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25700 FvH4_2g11190 FvH4_2g11201 FvH4_2g16130 FvH4_3g40630 FvH4_4g02210 FvH4_4g14564 FvH4_6g24041 FvH4_6g24050 FvH4_6g24050
rosa_chinensis RchiOBHm_Chr1g0348241 RchiOBHm_Chr2g0109211 RchiOBHm_Chr2g0144471 RchiOBHm_Chr2g0174771 RchiOBHm_Chr3g0495101 RchiOBHm_Chr5g0047301 RchiOBHm_Chr5g0062201
rosa_laevigata RLG00000000570 RLG00000000571 RLG00000008718 RLG00000008719 RLG00000008835 RLG00000019314 RLG00000019316 RLG00000020979
rosa_multiflora Rmu_co8024416.1_g000001 Rmu_co8152282.1_g000001 Rmu_sc0000103.1_g000058 Rmu_sc0000161.1_g000022 Rmu_sc0000517.1_g000009 Rmu_sc0000517.1_g000010 Rmu_sc0000571.1_g000024 Rmu_sc0000580.1_g000130 Rmu_sc0000744.1_g000130 Rmu_sc0000796.1_g000017 Rmu_sc0000800.1_g000021 Rmu_sc0000830.1_g000037 Rmu_sc0000830.1_g000038 Rmu_sc0000830.1_g000059 Rmu_sc0000958.1_g000012 Rmu_sc0001094.1_g000018 Rmu_sc0001163.1_g000004 Rmu_sc0001200.1_g000052 Rmu_sc0001339.1_g000005 Rmu_sc0001397.1_g000009 Rmu_sc0001563.1_g000043 Rmu_sc0001563.1_g000044 Rmu_sc0001570.1_g000001 Rmu_sc0001629.1_g000016 Rmu_sc0001763.1_g000006 Rmu_sc0002011.1_g000013 Rmu_sc0002012.1_g000012 Rmu_sc0002268.1_g000026 Rmu_sc0002268.1_g000027 Rmu_sc0002321.1_g000021 Rmu_sc0002722.1_g000002 Rmu_sc0003032.1_g000006 Rmu_sc0003185.1_g000008 Rmu_sc0003238.1_g000006 Rmu_sc0003275.1_g000037 Rmu_sc0003275.1_g000038 Rmu_sc0003441.1_g000042 Rmu_sc0003748.1_g000027 Rmu_sc0004229.1_g000011 Rmu_sc0004283.1_g000041 Rmu_sc0004283.1_g000042 Rmu_sc0004283.1_g000043 Rmu_sc0004841.1_g000026 Rmu_sc0004841.1_g000031 Rmu_sc0004877.1_g000001 Rmu_sc0005039.1_g000012 Rmu_sc0005584.1_g000019 Rmu_sc0005584.1_g000020 Rmu_sc0005584.1_g000021 Rmu_sc0005600.1_g000021 Rmu_sc0005914.1_g000004 Rmu_sc0006139.1_g000006 Rmu_sc0006638.1_g000001 Rmu_sc0007228.1_g000001 Rmu_sc0007300.1_g000008 Rmu_sc0007486.1_g000002 Rmu_sc0007737.1_g000007 Rmu_sc0008328.1_g000015 Rmu_sc0009064.1_g000002 Rmu_sc0009315.1_g000006 Rmu_sc0009472.1_g000009 Rmu_sc0009774.1_g000016 Rmu_sc0009858.1_g000010 Rmu_sc0009858.1_g000011 Rmu_sc0010489.1_g000001 Rmu_sc0010579.1_g000002 Rmu_sc0011511.1_g000002 Rmu_sc0013359.1_g000001 Rmu_sc0014367.1_g000005 Rmu_sc0015624.1_g000003 Rmu_sc0016756.1_g000001 Rmu_sc0021286.1_g000001 Rmu_sc0021693.1_g000001 Rmu_sc0022538.1_g000001 Rmu_sc0030983.1_g000001 Rmu_sc0037682.1_g000001 Rmu_sc0041059.1_g000001 Rmu_ssc0000003.1_g000013 Rmu_ssc0000003.1_g000015 Rmu_ssc0000388.1_g000018 Rmu_ssc0000409.1_g000013 Rmu_ssc0000409.1_g000015 Rmu_ssc0000476.1_g000003
rosa_roxburghii Rroxscaffold_1G00017510 Rroxscaffold_1G00017520 Rroxscaffold_1G00017530 Rroxscaffold_1G00017990 Rroxscaffold_1G00031430 Rroxscaffold_1G00045790 Rroxscaffold_2G00108360 Rroxscaffold_2G00127910 Rroxscaffold_3G00218970 Rroxscaffold_3G00218980 Rroxscaffold_3G00218990 Rroxscaffold_3G00226450 Rroxscaffold_3G00226460 Rroxscaffold_3G00230570 Rroxscaffold_3G00230920 Rroxscaffold_4G00284360 Rroxscaffold_4G00292370 Rroxscaffold_4G00307090 Rroxscaffold_4G00319260 Rroxscaffold_4G00332580 Rroxscaffold_5G00341080 Rroxscaffold_5G00344620 Rroxscaffold_5G00358950 Rroxscaffold_5G00368600 Rroxscaffold_5G00368610 Rroxscaffold_5G00378350 Rroxscaffold_6G00388550 Rroxscaffold_6G00398560 Rroxscaffold_6G00398570 Rroxscaffold_7G00194900 Rroxscaffold_7G00194910 Rroxscaffold_7G00194920
rosa_rugosa Rorug05G0347200 Rorug06G0064400 Rorug06G0064500 Rorug07G0190700 Rorug07G0190800 Rorug07G0276200 Rorug07G0344800 Rorug07G0344900
rosa_samantha Rh1AG138000 Rh1AG138100 Rh1DG079100 Rh1DG264500 Rh1DG264600 Rh1DG275600 Rh1DG275700 Rh2AG474800 Rh2AG474900 Rh2AG557100 Rh2AG557200 Rh2AG567400 Rh2AG568000 Rh2AG568100 Rh3CG345000 Rh3CG351000 Rh3CG364300 Rh4AG186000 Rh5AG278000 Rh5DG280000 Rh5DG281700 Rh5DG447800 Rh7AG499400 Rh7BG470300 Rh7BG470400 Rh7DG197800 Rh7DG197900 Rh7DG198000 Rh7DG284800 Rh7DG311600 Rh7DG311700 Rh7DG329300 Rh7DG393300 Rh7DG393500 Rh7DG483800 Rh7DG483900
rosa_wichuraiana Rw0G003510 Rw1G021670 Rw2G016920 Rw3G020700 Rw7G028090 Rw7G031160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 455
AccB1I GGYRCC 1 cut(s) 224
AccBSI CCGCTC 2 cut(s) 111, 606
AccI GTMKAC 1 cut(s) 15
AccII CGCG 1 cut(s) 69
AciI CCGC 6 cut(s) 36, 67, 69, 109, 557, 606
AclWI GGATC 1 cut(s) 791
AcuI CTGAAG 2 cut(s) 227, 798
AcyI GRCGYC 1 cut(s) 64
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 3 cut(s) 88, 383, 463
AflIII ACRYGT 1 cut(s) 867
AgsI TTSAA 3 cut(s) 241, 293, 749
AluBI AGCT 8 cut(s) 28, 154, 302, 726, 740, 752, 782, 850
AluI AGCT 8 cut(s) 28, 154, 302, 726, 740, 752, 782, 850
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 4 cut(s) 92, 152, 437, 576
AlwI GGATC 1 cut(s) 791
AlwNI CAGNNNCTG 1 cut(s) 305
AoxI GGCC 1 cut(s) 70
ApeKI GCWGC 6 cut(s) 302, 305, 726, 758, 782, 785
AsuII TTCGAA 1 cut(s) 76
BanI GGYRCC 1 cut(s) 224
BanII GRGCYC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 6 cut(s) 289, 292, 713, 745, 769, 772
BceAI ACGGC 1 cut(s) 100
BcgI CGANNNNNNTGC 2 cut(s) 708, 742
BciVI GTATCC 2 cut(s) 661, 761
BcoDI GTCTC 4 cut(s) 92, 152, 437, 576
BfaI CTAG 1 cut(s) 861
BfuAI ACCTGC 1 cut(s) 455
BfuI GTATCC 2 cut(s) 661, 761
BisI GCNGC 9 cut(s) 36, 67, 70, 303, 306, 727, 759, 783, 786
BlsI GCNGC 9 cut(s) 37, 68, 71, 304, 307, 728, 760, 784, 787
BmiI GGNNCC 2 cut(s) 43, 226
BmrI ACTGGG 1 cut(s) 469
BmsI GCATC 3 cut(s) 241, 397, 509
BmuI ACTGGG 1 cut(s) 469
BplI GAGNNNNNCTC 2 cut(s) 38, 70
BpmI CTGGAG 2 cut(s) 270, 751
Bpu14I TTCGAA 1 cut(s) 76
BpuEI CTTGAG 1 cut(s) 176
BsaHI GRCGYC 1 cut(s) 64
BsaI GGTCTC 1 cut(s) 437
BsaJI CCNNGG 3 cut(s) 67, 82, 402
BsaXI ACNNNNNCTCC 4 cut(s) 417, 447, 684, 714
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 383, 463
Bse1I ACTGG 3 cut(s) 287, 354, 464
BseDI CCNNGG 3 cut(s) 67, 82, 402
BseGI GGATG 1 cut(s) 412
BseLI CCNNNNNNNGG 3 cut(s) 88, 383, 463
BseMII CTCAG 2 cut(s) 323, 327
BseNI ACTGG 3 cut(s) 287, 354, 464
BseRI GAGGAG 2 cut(s) 115, 441
BseXI GCAGC 6 cut(s) 289, 292, 713, 745, 769, 772
BseYI CCCAGC 2 cut(s) 87, 298
Bsh1236I CGCG 1 cut(s) 69
BshFI GGCC 1 cut(s) 72
BshNI GGYRCC 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 2 cut(s) 39, 173
BsiWI CGTACG 1 cut(s) 378
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 3 cut(s) 88, 383, 463
BsmAI GTCTC 4 cut(s) 92, 152, 437, 576
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 1 cut(s) 72
Bso31I GGTCTC 1 cut(s) 437
Bsp119I TTCGAA 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 30
Bsp143I GATC 1 cut(s) 796
BspACI CCGC 6 cut(s) 36, 67, 69, 109, 557, 606
BspANI GGCC 1 cut(s) 72
BspCNI CTCAG 2 cut(s) 322, 328
BspFNI CGCG 1 cut(s) 69
BspHI TCATGA 1 cut(s) 831
BspLI GGNNCC 2 cut(s) 43, 226
BspMI ACCTGC 1 cut(s) 455
BspPI GGATC 1 cut(s) 791
BspT104I TTCGAA 1 cut(s) 76
BspT107I GGYRCC 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 437
BsrBI CCGCTC 2 cut(s) 111, 606
BsrI ACTGG 3 cut(s) 287, 354, 464
BssECI CCNNGG 3 cut(s) 67, 82, 402
BssMI GATC 1 cut(s) 796
BssNI GRCGYC 1 cut(s) 64
BssT1I CCWWGG 1 cut(s) 402
Bst4CI ACNGT 4 cut(s) 141, 378, 583, 683
Bst6I CTCTTC 2 cut(s) 212, 579
BstACI GRCGYC 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 76
BstDEI CTNAG 4 cut(s) 309, 336, 438, 635
BstDSI CCRYGG 2 cut(s) 67, 82
BstF5I GGATG 1 cut(s) 412
BstFNI CGCG 1 cut(s) 69
BstKTI GATC 1 cut(s) 799
BstMAI GTCTC 4 cut(s) 92, 152, 437, 576
BstMBI GATC 1 cut(s) 796
BstMWI GCNNNNNNNGC 4 cut(s) 41, 251, 631, 758
BstNSI RCATGY 3 cut(s) 172, 215, 871
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 6 cut(s) 289, 292, 713, 745, 769, 772
BsuI GTATCC 2 cut(s) 661, 761
BsuRI GGCC 1 cut(s) 72
BtgI CCRYGG 2 cut(s) 67, 82
BtsCI GGATG 1 cut(s) 412
BtsIMutI CAGTG 3 cut(s) 317, 457, 679
BveI ACCTGC 1 cut(s) 455
CaiI CAGNNNCTG 1 cut(s) 305
CciI TCATGA 1 cut(s) 831
Cfr42I CCGCGG 1 cut(s) 70
CseI GACGC 1 cut(s) 72
Csp6I GTAC 1 cut(s) 379
CviAII CATG 5 cut(s) 169, 212, 598, 832, 868
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 4 cut(s) 309, 336, 438, 635
DpnI GATC 1 cut(s) 798
DpnII GATC 1 cut(s) 796
Eam1104I CTCTTC 2 cut(s) 212, 579
EarI CTCTTC 2 cut(s) 212, 579
EciI GGCGGA 1 cut(s) 572
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 402
Eco24I GRGCYC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 437
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 2 cut(s) 227, 798
EcoICRI GAGCTC 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 5 cut(s) 172, 215, 601, 835, 871
FaqI GGGAC 1 cut(s) 281
FatI CATG 5 cut(s) 168, 211, 597, 831, 867
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 9 cut(s) 36, 67, 70, 303, 306, 727, 759, 783, 786
FokI GGATG 1 cut(s) 419
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 9 cut(s) 36, 67, 70, 303, 306, 727, 759, 783, 786
FspBI CTAG 1 cut(s) 861
GluI GCNGC 9 cut(s) 36, 67, 70, 303, 306, 727, 759, 783, 786
GsaI CCCAGC 2 cut(s) 91, 302
GsuI CTGGAG 2 cut(s) 270, 751
HaeIII GGCC 1 cut(s) 72
HapII CCGG 2 cut(s) 39, 173
HgaI GACGC 1 cut(s) 72
Hin1I GRCGYC 1 cut(s) 64
Hin1II CATG 5 cut(s) 172, 215, 601, 835, 871
HincII GTYRAC 2 cut(s) 16, 643
HindII GTYRAC 2 cut(s) 16, 643
HindIII AAGCTT 2 cut(s) 152, 750
HinfI GANTC 3 cut(s) 78, 100, 594
HpaII CCGG 2 cut(s) 39, 173
Hpy166II GTNNAC 3 cut(s) 16, 329, 643
Hpy188I TCNGA 6 cut(s) 58, 207, 258, 323, 337, 778
Hpy188III TCNNGA 3 cut(s) 97, 719, 832
Hpy8I GTNNAC 3 cut(s) 16, 329, 643
Hpy99I CGWCG 2 cut(s) 20, 62
HpyAV CCTTC 5 cut(s) 16, 83, 265, 416, 783
HpyCH4III ACNGT 4 cut(s) 141, 378, 583, 683
HpyCH4V TGCA 6 cut(s) 410, 450, 471, 494, 679, 761
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 251, 631, 758
HpyF3I CTNAG 4 cut(s) 309, 336, 438, 635
Hsp92I GRCGYC 1 cut(s) 64
Hsp92II CATG 5 cut(s) 172, 215, 601, 835, 871
KspI CCGCGG 1 cut(s) 70
Kzo9I GATC 1 cut(s) 796
LmnI GCTCC 3 cut(s) 25, 41, 116
Lsp1109I GCAGC 6 cut(s) 289, 292, 713, 745, 769, 772
LweI GCATC 3 cut(s) 241, 397, 509
MaeI CTAG 1 cut(s) 861
MaeIII GTNAC 2 cut(s) 164, 863
MalI GATC 1 cut(s) 798
MbiI CCGCTC 2 cut(s) 111, 606
MboI GATC 1 cut(s) 796
MboII GAAGA 6 cut(s) 229, 280, 557, 566, 608, 707
MhlI GDGCHC 1 cut(s) 30
MluCI AATT 2 cut(s) 480, 551
MlyI GAGTC 2 cut(s) 94, 603
MmeI TCCRAC 1 cut(s) 81
MseI TTAA 1 cut(s) 662
MslI CAYNNNNRTG 1 cut(s) 240
MspA1I CMGCKG 2 cut(s) 69, 302
MspI CCGG 2 cut(s) 39, 173
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 4 cut(s) 41, 251, 631, 758
NdeII GATC 1 cut(s) 796
NlaIII CATG 5 cut(s) 172, 215, 601, 835, 871
NlaIV GGNNCC 2 cut(s) 43, 226
NmeAIII GCCGAG 2 cut(s) 70, 627
NmuCI GTSAC 1 cut(s) 164
NspI RCATGY 3 cut(s) 172, 215, 871
NspV TTCGAA 1 cut(s) 76
PagI TCATGA 1 cut(s) 831
PciI ACATGT 1 cut(s) 867
PfeI GAWTC 1 cut(s) 78
Pfl23II CGTACG 1 cut(s) 378
PkrI GCNGC 9 cut(s) 37, 68, 71, 304, 307, 728, 760, 784, 787
PleI GAGTC 2 cut(s) 94, 602
PpsI GAGTC 2 cut(s) 94, 602
PscI ACATGT 1 cut(s) 867
Psp124BI GAGCTC 1 cut(s) 30
PspFI CCCAGC 2 cut(s) 87, 298
PspLI CGTACG 1 cut(s) 378
PspN4I GGNNCC 2 cut(s) 43, 226
PstNI CAGNNNCTG 1 cut(s) 305
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
RseI CAYNNNNRTG 1 cut(s) 240
SacI GAGCTC 1 cut(s) 30
SacII CCGCGG 1 cut(s) 70
SalI GTCGAC 1 cut(s) 14
SaqAI TTAA 1 cut(s) 662
SatI GCNGC 9 cut(s) 36, 67, 70, 303, 306, 727, 759, 783, 786
Sau3AI GATC 1 cut(s) 796
SchI GAGTC 2 cut(s) 94, 603
SduI GDGCHC 1 cut(s) 30
SfaNI GCATC 3 cut(s) 241, 397, 509
Sfr303I CCGCGG 1 cut(s) 70
SfuI TTCGAA 1 cut(s) 76
SgrBI CCGCGG 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 2 cut(s) 480, 551
SsiI CCGC 6 cut(s) 36, 67, 69, 109, 557, 606
SspMI CTAG 1 cut(s) 861
SstI GAGCTC 1 cut(s) 30
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 4 cut(s) 141, 378, 583, 683
TaqI TCGA 4 cut(s) 15, 76, 96, 718
TasI AATT 2 cut(s) 480, 551
TauI GCSGC 3 cut(s) 38, 69, 72
TfiI GAWTC 1 cut(s) 78
Tru1I TTAA 1 cut(s) 662
Tru9I TTAA 1 cut(s) 662
TscAI CASTG 3 cut(s) 317, 464, 686
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 6 cut(s) 302, 305, 726, 758, 782, 785
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 2 cut(s) 558, 564
TspGWI ACGGA 3 cut(s) 75, 396, 660
TspRI CASTG 3 cut(s) 317, 464, 686
XceI RCATGY 3 cut(s) 172, 215, 871
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 1 cut(s) 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.