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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 273, 289 |
| AclWI | GGATC | 2 cut(s) | 184, 326 |
| AcsI | RAATTY | 1 cut(s) | 50 |
| AluBI | AGCT | 1 cut(s) | 26 |
| AluI | AGCT | 1 cut(s) | 26 |
| Alw21I | GWGCWC | 2 cut(s) | 34, 40 |
| AlwI | GGATC | 2 cut(s) | 184, 326 |
| ApeKI | GCWGC | 3 cut(s) | 56, 131, 246 |
| ApoI | RAATTY | 1 cut(s) | 50 |
| AspS9I | GGNCC | 1 cut(s) | 68 |
| AsuHPI | GGTGA | 1 cut(s) | 130 |
| AvaII | GGWCC | 1 cut(s) | 68 |
| AxyI | CCTNAGG | 1 cut(s) | 225 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 88, 121 |
| BanII | GRGCYC | 1 cut(s) | 216 |
| BauI | CACGAG | 2 cut(s) | 11, 33 |
| Bbv12I | GWGCWC | 2 cut(s) | 34, 40 |
| BbvI | GCAGC | 3 cut(s) | 43, 118, 233 |
| BccI | CCATC | 1 cut(s) | 225 |
| BfmI | CTRYAG | 1 cut(s) | 132 |
| BisI | GCNGC | 3 cut(s) | 57, 132, 247 |
| BlsI | GCNGC | 3 cut(s) | 58, 133, 248 |
| Bme18I | GGWCC | 1 cut(s) | 68 |
| BmgT120I | GGNCC | 1 cut(s) | 68 |
| BmiI | GGNNCC | 1 cut(s) | 271 |
| BoxI | GACNNNNGTC | 1 cut(s) | 141 |
| Bse21I | CCTNAGG | 1 cut(s) | 225 |
| BseGI | GGATG | 1 cut(s) | 160 |
| BseMII | CTCAG | 2 cut(s) | 216, 305 |
| BseXI | GCAGC | 3 cut(s) | 43, 118, 233 |
| BsgI | GTGCAG | 1 cut(s) | 96 |
| BsiHKAI | GWGCWC | 2 cut(s) | 34, 40 |
| BslFI | GGGAC | 2 cut(s) | 214, 243 |
| BsmFI | GGGAC | 2 cut(s) | 214, 243 |
| Bsp1286I | GDGCHC | 3 cut(s) | 34, 40, 216 |
| Bsp143I | GATC | 2 cut(s) | 176, 318 |
| BspACI | CCGC | 2 cut(s) | 273, 289 |
| BspCNI | CTCAG | 2 cut(s) | 217, 306 |
| BspLI | GGNNCC | 1 cut(s) | 271 |
| BspMAI | CTGCAG | 1 cut(s) | 136 |
| BspPI | GGATC | 2 cut(s) | 184, 326 |
| BssMI | GATC | 2 cut(s) | 176, 318 |
| BssSI | CACGAG | 2 cut(s) | 11, 33 |
| Bst2BI | CACGAG | 2 cut(s) | 11, 33 |
| Bst4CI | ACNGT | 2 cut(s) | 86, 193 |
| BstDEI | CTNAG | 2 cut(s) | 225, 314 |
| BstF5I | GGATG | 1 cut(s) | 160 |
| BstKTI | GATC | 2 cut(s) | 179, 321 |
| BstMBI | GATC | 2 cut(s) | 176, 318 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 141 |
| BstSFI | CTRYAG | 1 cut(s) | 132 |
| BstV1I | GCAGC | 3 cut(s) | 43, 118, 233 |
| Bsu36I | CCTNAGG | 1 cut(s) | 225 |
| BtsCI | GGATG | 1 cut(s) | 160 |
| BtsIMutI | CAGTG | 2 cut(s) | 115, 311 |
| Cfr13I | GGNCC | 1 cut(s) | 68 |
| CviJI | RGCY | 2 cut(s) | 26, 214 |
| CviKI_1 | RGCY | 2 cut(s) | 26, 214 |
| DdeI | CTNAG | 2 cut(s) | 225, 314 |
| DpnI | GATC | 2 cut(s) | 178, 320 |
| DpnII | GATC | 2 cut(s) | 176, 318 |
| Eco24I | GRGCYC | 1 cut(s) | 216 |
| Eco47I | GGWCC | 1 cut(s) | 68 |
| Eco81I | CCTNAGG | 1 cut(s) | 225 |
| EcoT38I | GRGCYC | 1 cut(s) | 216 |
| FaiI | YATR | 3 cut(s) | 18, 162, 264 |
| FaqI | GGGAC | 2 cut(s) | 214, 243 |
| Fnu4HI | GCNGC | 3 cut(s) | 57, 132, 247 |
| FokI | GGATG | 1 cut(s) | 167 |
| FriOI | GRGCYC | 1 cut(s) | 216 |
| Fsp4HI | GCNGC | 3 cut(s) | 57, 132, 247 |
| GluI | GCNGC | 3 cut(s) | 57, 132, 247 |
| HincII | GTYRAC | 1 cut(s) | 189 |
| HindII | GTYRAC | 1 cut(s) | 189 |
| HinfI | GANTC | 2 cut(s) | 113, 125 |
| HphI | GGTGA | 1 cut(s) | 130 |
| Hpy166II | GTNNAC | 1 cut(s) | 189 |
| Hpy188I | TCNGA | 1 cut(s) | 42 |
| Hpy188III | TCNNGA | 1 cut(s) | 11 |
| Hpy8I | GTNNAC | 1 cut(s) | 189 |
| HpyAV | CCTTC | 2 cut(s) | 40, 228 |
| HpyCH4III | ACNGT | 2 cut(s) | 86, 193 |
| HpyCH4IV | ACGT | 1 cut(s) | 99 |
| HpyCH4V | TGCA | 4 cut(s) | 59, 77, 134, 249 |
| HpyF3I | CTNAG | 2 cut(s) | 225, 314 |
| HpySE526I | ACGT | 1 cut(s) | 99 |
| Kzo9I | GATC | 2 cut(s) | 176, 318 |
| LpnPI | CCDG | 4 cut(s) | 12, 80, 155, 237 |
| Lsp1109I | GCAGC | 3 cut(s) | 43, 118, 233 |
| MaeII | ACGT | 1 cut(s) | 99 |
| MaeIII | GTNAC | 3 cut(s) | 86, 110, 239 |
| MalI | GATC | 2 cut(s) | 178, 320 |
| MboI | GATC | 2 cut(s) | 176, 318 |
| MhlI | GDGCHC | 3 cut(s) | 34, 40, 216 |
| MluCI | AATT | 1 cut(s) | 50 |
| MlyI | GAGTC | 1 cut(s) | 107 |
| MmeI | TCCRAC | 1 cut(s) | 175 |
| MnlI | CCTC | 5 cut(s) | 145, 191, 220, 296, 309 |
| MspA1I | CMGCKG | 1 cut(s) | 275 |
| NdeII | GATC | 2 cut(s) | 176, 318 |
| NlaIV | GGNNCC | 1 cut(s) | 271 |
| NmuCI | GTSAC | 1 cut(s) | 110 |
| PfeI | GAWTC | 1 cut(s) | 125 |
| PkrI | GCNGC | 3 cut(s) | 58, 133, 248 |
| PleI | GAGTC | 1 cut(s) | 107 |
| PpsI | GAGTC | 1 cut(s) | 107 |
| PshAI | GACNNNNGTC | 1 cut(s) | 141 |
| PspN4I | GGNNCC | 1 cut(s) | 271 |
| PspPI | GGNCC | 1 cut(s) | 68 |
| PstI | CTGCAG | 1 cut(s) | 136 |
| SatI | GCNGC | 3 cut(s) | 57, 132, 247 |
| Sau3AI | GATC | 2 cut(s) | 176, 318 |
| Sau96I | GGNCC | 1 cut(s) | 68 |
| SchI | GAGTC | 1 cut(s) | 107 |
| SduI | GDGCHC | 3 cut(s) | 34, 40, 216 |
| SetI | ASST | 2 cut(s) | 28, 102 |
| SfcI | CTRYAG | 1 cut(s) | 132 |
| SinI | GGWCC | 1 cut(s) | 68 |
| Sse9I | AATT | 1 cut(s) | 50 |
| SsiI | CCGC | 2 cut(s) | 273, 289 |
| TaaI | ACNGT | 2 cut(s) | 86, 193 |
| TaiI | ACGT | 1 cut(s) | 102 |
| TaqII | GACCGA | 1 cut(s) | 56 |
| TasI | AATT | 1 cut(s) | 50 |
| TfiI | GAWTC | 1 cut(s) | 125 |
| TscAI | CASTG | 2 cut(s) | 115, 318 |
| TseFI | GTSAC | 1 cut(s) | 110 |
| TseI | GCWGC | 3 cut(s) | 56, 131, 246 |
| Tsp45I | GTSAC | 1 cut(s) | 110 |
| TspDTI | ATGAA | 1 cut(s) | 59 |
| TspRI | CASTG | 2 cut(s) | 115, 318 |
| VpaK11BI | GGWCC | 1 cut(s) | 68 |
| XapI | RAATTY | 1 cut(s) | 50 |