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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 785 |
| AccI | GTMKAC | 2 cut(s) | 156, 225 |
| AciI | CCGC | 2 cut(s) | 322, 514 |
| AclWI | GGATC | 1 cut(s) | 354 |
| AcsI | RAATTY | 1 cut(s) | 806 |
| AfaI | GTAC | 1 cut(s) | 667 |
| AgsI | TTSAA | 2 cut(s) | 152, 674 |
| AjnI | CCWGG | 1 cut(s) | 699 |
| AluBI | AGCT | 3 cut(s) | 450, 467, 707 |
| AluI | AGCT | 3 cut(s) | 450, 467, 707 |
| AlwI | GGATC | 1 cut(s) | 354 |
| AoxI | GGCC | 2 cut(s) | 455, 823 |
| ApeKI | GCWGC | 1 cut(s) | 812 |
| ApoI | RAATTY | 1 cut(s) | 806 |
| AspS9I | GGNCC | 1 cut(s) | 749 |
| AsuHPI | GGTGA | 5 cut(s) | 179, 266, 352, 641, 811 |
| AvaII | GGWCC | 1 cut(s) | 749 |
| AxyI | CCTNAGG | 2 cut(s) | 333, 509 |
| BbvI | GCAGC | 1 cut(s) | 799 |
| BccI | CCATC | 3 cut(s) | 409, 410, 812 |
| BceAI | ACGGC | 2 cut(s) | 541, 709 |
| BciT130I | CCWGG | 1 cut(s) | 701 |
| BfaI | CTAG | 1 cut(s) | 447 |
| BfmI | CTRYAG | 2 cut(s) | 738, 813 |
| BisI | GCNGC | 2 cut(s) | 322, 813 |
| BlsI | GCNGC | 2 cut(s) | 323, 814 |
| BmcAI | AGTACT | 1 cut(s) | 667 |
| Bme1390I | CCNGG | 1 cut(s) | 701 |
| Bme18I | GGWCC | 1 cut(s) | 749 |
| BmgT120I | GGNCC | 1 cut(s) | 749 |
| BmrFI | CCNGG | 1 cut(s) | 701 |
| BmsI | GCATC | 4 cut(s) | 73, 360, 481, 705 |
| BpmI | CTGGAG | 1 cut(s) | 345 |
| Bpu10I | CCTNAGC | 1 cut(s) | 564 |
| BpuEI | CTTGAG | 2 cut(s) | 176, 472 |
| Bsa29I | ATCGAT | 1 cut(s) | 45 |
| BsaJI | CCNNGG | 1 cut(s) | 625 |
| Bse1I | ACTGG | 2 cut(s) | 362, 663 |
| Bse21I | CCTNAGG | 2 cut(s) | 333, 509 |
| BseBI | CCWGG | 1 cut(s) | 701 |
| BseCI | ATCGAT | 1 cut(s) | 45 |
| BseDI | CCNNGG | 1 cut(s) | 625 |
| BseGI | GGATG | 3 cut(s) | 217, 351, 696 |
| BseMII | CTCAG | 1 cut(s) | 125 |
| BseNI | ACTGG | 2 cut(s) | 362, 663 |
| BseRI | GAGGAG | 1 cut(s) | 348 |
| BseXI | GCAGC | 1 cut(s) | 799 |
| BshFI | GGCC | 2 cut(s) | 457, 825 |
| BshVI | ATCGAT | 1 cut(s) | 45 |
| BsnI | GGCC | 2 cut(s) | 457, 825 |
| Bsp143I | GATC | 3 cut(s) | 46, 346, 583 |
| BspACI | CCGC | 2 cut(s) | 322, 514 |
| BspANI | GGCC | 2 cut(s) | 457, 825 |
| BspCNI | CTCAG | 1 cut(s) | 124 |
| BspDI | ATCGAT | 1 cut(s) | 45 |
| BspMAI | CTGCAG | 1 cut(s) | 817 |
| BspPI | GGATC | 1 cut(s) | 354 |
| BsrI | ACTGG | 2 cut(s) | 362, 663 |
| BssECI | CCNNGG | 1 cut(s) | 625 |
| BssMI | GATC | 3 cut(s) | 46, 346, 583 |
| BssNAI | GTATAC | 1 cut(s) | 157 |
| BssT1I | CCWWGG | 1 cut(s) | 625 |
| Bst1107I | GTATAC | 1 cut(s) | 157 |
| Bst2UI | CCWGG | 1 cut(s) | 701 |
| Bst4CI | ACNGT | 5 cut(s) | 134, 391, 502, 611, 767 |
| BstDEI | CTNAG | 6 cut(s) | 111, 333, 509, 552, 564, 587 |
| BstEII | GGTNACC | 1 cut(s) | 185 |
| BstF5I | GGATG | 3 cut(s) | 217, 351, 696 |
| BstKTI | GATC | 3 cut(s) | 49, 349, 586 |
| BstMBI | GATC | 3 cut(s) | 46, 346, 583 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 569 |
| BstNI | CCWGG | 1 cut(s) | 701 |
| BstPI | GGTNACC | 1 cut(s) | 185 |
| BstSCI | CCNGG | 1 cut(s) | 699 |
| BstSFI | CTRYAG | 2 cut(s) | 738, 813 |
| BstV1I | GCAGC | 1 cut(s) | 799 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 707 |
| BstZ17I | GTATAC | 1 cut(s) | 157 |
| Bsu15I | ATCGAT | 1 cut(s) | 45 |
| Bsu36I | CCTNAGG | 2 cut(s) | 333, 509 |
| BsuRI | GGCC | 2 cut(s) | 457, 825 |
| BsuTUI | ATCGAT | 1 cut(s) | 45 |
| BtgZI | GCGATG | 1 cut(s) | 45 |
| BtsCI | GGATG | 3 cut(s) | 217, 351, 696 |
| BtsIMutI | CAGTG | 3 cut(s) | 139, 396, 616 |
| Cfr13I | GGNCC | 1 cut(s) | 749 |
| ClaI | ATCGAT | 1 cut(s) | 45 |
| Csp6I | GTAC | 1 cut(s) | 666 |
| CviAII | CATG | 3 cut(s) | 488, 524, 535 |
| CviQI | GTAC | 1 cut(s) | 666 |
| DdeI | CTNAG | 6 cut(s) | 111, 333, 509, 552, 564, 587 |
| DpnI | GATC | 3 cut(s) | 48, 348, 585 |
| DpnII | GATC | 3 cut(s) | 46, 346, 583 |
| DraI | TTTAAA | 1 cut(s) | 328 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 785 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 785 |
| EciI | GGCGGA | 1 cut(s) | 529 |
| Eco130I | CCWWGG | 1 cut(s) | 625 |
| Eco47I | GGWCC | 1 cut(s) | 749 |
| Eco81I | CCTNAGG | 2 cut(s) | 333, 509 |
| Eco91I | GGTNACC | 1 cut(s) | 185 |
| EcoO65I | GGTNACC | 1 cut(s) | 185 |
| EcoRII | CCWGG | 1 cut(s) | 699 |
| EcoT14I | CCWWGG | 1 cut(s) | 625 |
| ErhI | CCWWGG | 1 cut(s) | 625 |
| FaeI | CATG | 3 cut(s) | 491, 527, 538 |
| FatI | CATG | 3 cut(s) | 487, 523, 534 |
| FblI | GTMKAC | 2 cut(s) | 156, 225 |
| Fnu4HI | GCNGC | 2 cut(s) | 322, 813 |
| FokI | GGATG | 3 cut(s) | 224, 338, 683 |
| Fsp4HI | GCNGC | 2 cut(s) | 322, 813 |
| FspBI | CTAG | 1 cut(s) | 447 |
| GluI | GCNGC | 2 cut(s) | 322, 813 |
| GsuI | CTGGAG | 1 cut(s) | 345 |
| HaeIII | GGCC | 2 cut(s) | 457, 825 |
| Hin1II | CATG | 3 cut(s) | 491, 527, 538 |
| HinfI | GANTC | 2 cut(s) | 659, 731 |
| HphI | GGTGA | 5 cut(s) | 179, 266, 352, 641, 811 |
| Hpy166II | GTNNAC | 3 cut(s) | 80, 157, 226 |
| Hpy188I | TCNGA | 2 cut(s) | 114, 867 |
| Hpy188III | TCNNGA | 1 cut(s) | 50 |
| Hpy8I | GTNNAC | 3 cut(s) | 80, 157, 226 |
| HpyAV | CCTTC | 4 cut(s) | 244, 307, 337, 721 |
| HpyCH4III | ACNGT | 5 cut(s) | 134, 391, 502, 611, 767 |
| HpyCH4V | TGCA | 3 cut(s) | 96, 266, 815 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 569 |
| HpyF3I | CTNAG | 6 cut(s) | 111, 333, 509, 552, 564, 587 |
| Hsp92II | CATG | 3 cut(s) | 491, 527, 538 |
| Kzo9I | GATC | 3 cut(s) | 46, 346, 583 |
| LpnPI | CCDG | 8 cut(s) | 35, 268, 375, 676, 686, 693, 713, 761 |
| Lsp1109I | GCAGC | 1 cut(s) | 799 |
| LweI | GCATC | 4 cut(s) | 73, 360, 481, 705 |
| MaeI | CTAG | 1 cut(s) | 447 |
| MaeIII | GTNAC | 7 cut(s) | 185, 235, 254, 385, 481, 590, 767 |
| MalI | GATC | 3 cut(s) | 48, 348, 585 |
| MboI | GATC | 3 cut(s) | 46, 346, 583 |
| MboII | GAAGA | 2 cut(s) | 664, 686 |
| MluCI | AATT | 2 cut(s) | 806, 868 |
| MnlI | CCTC | 9 cut(s) | 24, 200, 202, 214, 366, 369, 447, 607, 751 |
| MseI | TTAA | 5 cut(s) | 18, 27, 140, 327, 471 |
| MspR9I | CCNGG | 1 cut(s) | 701 |
| MvaI | CCWGG | 1 cut(s) | 701 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 569 |
| NdeII | GATC | 3 cut(s) | 46, 346, 583 |
| NlaIII | CATG | 3 cut(s) | 491, 527, 538 |
| NmuCI | GTSAC | 3 cut(s) | 185, 254, 590 |
| PfeI | GAWTC | 2 cut(s) | 659, 731 |
| PkrI | GCNGC | 2 cut(s) | 323, 814 |
| Psp6I | CCWGG | 1 cut(s) | 699 |
| PspEI | GGTNACC | 1 cut(s) | 185 |
| PspGI | CCWGG | 1 cut(s) | 699 |
| PspPI | GGNCC | 1 cut(s) | 749 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 846, 878 |
| PstI | CTGCAG | 1 cut(s) | 817 |
| RsaI | GTAC | 1 cut(s) | 667 |
| RsaNI | GTAC | 1 cut(s) | 666 |
| SaqAI | TTAA | 5 cut(s) | 18, 27, 140, 327, 471 |
| SatI | GCNGC | 2 cut(s) | 322, 813 |
| Sau3AI | GATC | 3 cut(s) | 46, 346, 583 |
| Sau96I | GGNCC | 1 cut(s) | 749 |
| ScaI | AGTACT | 1 cut(s) | 667 |
| ScrFI | CCNGG | 1 cut(s) | 701 |
| SfaNI | GCATC | 4 cut(s) | 73, 360, 481, 705 |
| SfcI | CTRYAG | 2 cut(s) | 738, 813 |
| SinI | GGWCC | 1 cut(s) | 749 |
| SmlI | CTYRAG | 2 cut(s) | 191, 451 |
| SmoI | CTYRAG | 2 cut(s) | 191, 451 |
| Sse9I | AATT | 2 cut(s) | 806, 868 |
| SsiI | CCGC | 2 cut(s) | 322, 514 |
| SspMI | CTAG | 1 cut(s) | 447 |
| StyD4I | CCNGG | 1 cut(s) | 699 |
| StyI | CCWWGG | 1 cut(s) | 625 |
| TaaI | ACNGT | 5 cut(s) | 134, 391, 502, 611, 767 |
| TaqI | TCGA | 4 cut(s) | 45, 161, 404, 789 |
| TasI | AATT | 2 cut(s) | 806, 868 |
| TatI | WGTACW | 1 cut(s) | 665 |
| TauI | GCSGC | 1 cut(s) | 324 |
| TfiI | GAWTC | 2 cut(s) | 659, 731 |
| Tru1I | TTAA | 5 cut(s) | 18, 27, 140, 327, 471 |
| Tru9I | TTAA | 5 cut(s) | 18, 27, 140, 327, 471 |
| TscAI | CASTG | 3 cut(s) | 139, 396, 616 |
| TseFI | GTSAC | 3 cut(s) | 185, 254, 590 |
| TseI | GCWGC | 1 cut(s) | 812 |
| Tsp45I | GTSAC | 3 cut(s) | 185, 254, 590 |
| TspDTI | ATGAA | 1 cut(s) | 740 |
| TspRI | CASTG | 3 cut(s) | 139, 396, 616 |
| VpaK11BI | GGWCC | 1 cut(s) | 749 |
| XapI | RAATTY | 1 cut(s) | 806 |
| XmiI | GTMKAC | 2 cut(s) | 156, 225 |
| XspI | CTAG | 1 cut(s) | 447 |
| ZrmI | AGTACT | 1 cut(s) | 667 |