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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 138 |
| AccBSI | CCGCTC | 1 cut(s) | 181 |
| AciI | CCGC | 3 cut(s) | 89, 181, 236 |
| AfaI | GTAC | 2 cut(s) | 191, 267 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 90 |
| AgsI | TTSAA | 1 cut(s) | 284 |
| AjnI | CCWGG | 1 cut(s) | 164 |
| AluBI | AGCT | 3 cut(s) | 258, 280, 302 |
| AluI | AGCT | 3 cut(s) | 258, 280, 302 |
| Alw26I | GTCTC | 2 cut(s) | 74, 151 |
| AoxI | GGCC | 3 cut(s) | 93, 242, 288 |
| AspS9I | GGNCC | 3 cut(s) | 93, 228, 289 |
| AvaII | GGWCC | 1 cut(s) | 228 |
| BanI | GGYRCC | 1 cut(s) | 138 |
| BbsI | GAAGAC | 1 cut(s) | 12 |
| BccI | CCATC | 2 cut(s) | 59, 338 |
| BciT130I | CCWGG | 1 cut(s) | 166 |
| BciVI | GTATCC | 1 cut(s) | 228 |
| BcoDI | GTCTC | 2 cut(s) | 74, 151 |
| BfaI | CTAG | 1 cut(s) | 59 |
| BfmI | CTRYAG | 2 cut(s) | 30, 195 |
| BfuI | GTATCC | 1 cut(s) | 228 |
| Bme1390I | CCNGG | 1 cut(s) | 166 |
| Bme18I | GGWCC | 1 cut(s) | 228 |
| BmgT120I | GGNCC | 3 cut(s) | 93, 228, 289 |
| BmiI | GGNNCC | 2 cut(s) | 94, 140 |
| BmrFI | CCNGG | 1 cut(s) | 166 |
| BmsI | GCATC | 1 cut(s) | 128 |
| BpiI | GAAGAC | 1 cut(s) | 12 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 92, 124 |
| BpmI | CTGGAG | 1 cut(s) | 324 |
| BsaI | GGTCTC | 1 cut(s) | 74 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 291, 321 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 90 |
| BseBI | CCWGG | 1 cut(s) | 166 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 90 |
| BseMII | CTCAG | 2 cut(s) | 38, 123 |
| BseRI | GAGGAG | 1 cut(s) | 128 |
| BshFI | GGCC | 3 cut(s) | 95, 244, 290 |
| BshNI | GGYRCC | 1 cut(s) | 138 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 90 |
| BsmAI | GTCTC | 2 cut(s) | 74, 151 |
| BsnI | GGCC | 3 cut(s) | 95, 244, 290 |
| Bso31I | GGTCTC | 1 cut(s) | 74 |
| BspACI | CCGC | 3 cut(s) | 89, 181, 236 |
| BspANI | GGCC | 3 cut(s) | 95, 244, 290 |
| BspCNI | CTCAG | 2 cut(s) | 37, 122 |
| BspHI | TCATGA | 2 cut(s) | 40, 124 |
| BspLI | GGNNCC | 2 cut(s) | 94, 140 |
| BspMAI | CTGCAG | 1 cut(s) | 199 |
| BspQI | GCTCTTC | 1 cut(s) | 270 |
| BspT107I | GGYRCC | 1 cut(s) | 138 |
| BspTNI | GGTCTC | 1 cut(s) | 74 |
| BsrBI | CCGCTC | 1 cut(s) | 181 |
| Bst2UI | CCWGG | 1 cut(s) | 166 |
| Bst4CI | ACNGT | 1 cut(s) | 321 |
| Bst6I | CTCTTC | 2 cut(s) | 270, 279 |
| BstDEI | CTNAG | 3 cut(s) | 15, 24, 109 |
| BstMAI | GTCTC | 2 cut(s) | 74, 151 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 86, 178 |
| BstNI | CCWGG | 1 cut(s) | 166 |
| BstSCI | CCNGG | 1 cut(s) | 164 |
| BstSFI | CTRYAG | 2 cut(s) | 30, 195 |
| BstV2I | GAAGAC | 1 cut(s) | 12 |
| BsuI | GTATCC | 1 cut(s) | 228 |
| BsuRI | GGCC | 3 cut(s) | 95, 244, 290 |
| BtsI | GCAGTG | 2 cut(s) | 84, 204 |
| BtsIMutI | CAGTG | 3 cut(s) | 84, 204, 317 |
| CciI | TCATGA | 2 cut(s) | 40, 124 |
| Cfr13I | GGNCC | 3 cut(s) | 93, 228, 289 |
| Csp6I | GTAC | 2 cut(s) | 190, 266 |
| CviAII | CATG | 3 cut(s) | 41, 125, 371 |
| CviJI | RGCY | 7 cut(s) | 28, 95, 244, 258, 280, 290, 302 |
| CviKI_1 | RGCY | 7 cut(s) | 28, 95, 244, 258, 280, 290, 302 |
| CviQI | GTAC | 2 cut(s) | 190, 266 |
| DdeI | CTNAG | 3 cut(s) | 15, 24, 109 |
| Eam1104I | CTCTTC | 2 cut(s) | 270, 279 |
| EarI | CTCTTC | 2 cut(s) | 270, 279 |
| Eco31I | GGTCTC | 1 cut(s) | 74 |
| Eco47I | GGWCC | 1 cut(s) | 228 |
| EcoO109I | RGGNCCY | 3 cut(s) | 93, 228, 289 |
| EcoRII | CCWGG | 1 cut(s) | 164 |
| FaeI | CATG | 3 cut(s) | 44, 128, 374 |
| FaiI | YATR | 6 cut(s) | 42, 126, 161, 206, 208, 372 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 276, 308 |
| FatI | CATG | 3 cut(s) | 40, 124, 370 |
| FauI | CCCGC | 3 cut(s) | 82, 174, 229 |
| FspBI | CTAG | 1 cut(s) | 59 |
| GsuI | CTGGAG | 1 cut(s) | 324 |
| HaeIII | GGCC | 3 cut(s) | 95, 244, 290 |
| Hin1II | CATG | 3 cut(s) | 44, 128, 374 |
| Hpy166II | GTNNAC | 1 cut(s) | 346 |
| Hpy188I | TCNGA | 1 cut(s) | 112 |
| Hpy188III | TCNNGA | 2 cut(s) | 41, 125 |
| Hpy8I | GTNNAC | 1 cut(s) | 346 |
| HpyAV | CCTTC | 1 cut(s) | 374 |
| HpyCH4III | ACNGT | 1 cut(s) | 321 |
| HpyCH4V | TGCA | 2 cut(s) | 197, 224 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 86, 178 |
| HpyF3I | CTNAG | 3 cut(s) | 15, 24, 109 |
| Hsp92II | CATG | 3 cut(s) | 44, 128, 374 |
| LguI | GCTCTTC | 1 cut(s) | 270 |
| LmnI | GCTCC | 1 cut(s) | 299 |
| LweI | GCATC | 1 cut(s) | 128 |
| MaeI | CTAG | 1 cut(s) | 59 |
| MbiI | CCGCTC | 1 cut(s) | 181 |
| MboII | GAAGA | 5 cut(s) | 12, 74, 287, 296, 322 |
| MnlI | CCTC | 7 cut(s) | 94, 106, 109, 241, 256, 280, 334 |
| MspR9I | CCNGG | 1 cut(s) | 166 |
| MvaI | CCWGG | 1 cut(s) | 166 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 86, 178 |
| NlaIII | CATG | 3 cut(s) | 44, 128, 374 |
| NlaIV | GGNNCC | 2 cut(s) | 94, 140 |
| PagI | TCATGA | 2 cut(s) | 40, 124 |
| PciSI | GCTCTTC | 1 cut(s) | 270 |
| PpuMI | RGGWCCY | 1 cut(s) | 228 |
| Psp5II | RGGWCCY | 1 cut(s) | 228 |
| Psp6I | CCWGG | 1 cut(s) | 164 |
| PspGI | CCWGG | 1 cut(s) | 164 |
| PspN4I | GGNNCC | 2 cut(s) | 94, 140 |
| PspPI | GGNCC | 3 cut(s) | 93, 228, 289 |
| PspPPI | RGGWCCY | 1 cut(s) | 228 |
| PstI | CTGCAG | 1 cut(s) | 199 |
| RsaI | GTAC | 2 cut(s) | 191, 267 |
| RsaNI | GTAC | 2 cut(s) | 190, 266 |
| SapI | GCTCTTC | 1 cut(s) | 270 |
| Sau96I | GGNCC | 3 cut(s) | 93, 228, 289 |
| ScrFI | CCNGG | 1 cut(s) | 166 |
| SetI | ASST | 7 cut(s) | 170, 233, 260, 267, 282, 304, 345 |
| SfaNI | GCATC | 1 cut(s) | 128 |
| SfcI | CTRYAG | 2 cut(s) | 30, 195 |
| SinI | GGWCC | 1 cut(s) | 228 |
| SsiI | CCGC | 3 cut(s) | 89, 181, 236 |
| SspMI | CTAG | 1 cut(s) | 59 |
| StyD4I | CCNGG | 1 cut(s) | 164 |
| TaaI | ACNGT | 1 cut(s) | 321 |
| TscAI | CASTG | 3 cut(s) | 91, 204, 324 |
| TspDTI | ATGAA | 2 cut(s) | 29, 148 |
| TspRI | CASTG | 3 cut(s) | 91, 204, 324 |
| VpaK11BI | GGWCC | 1 cut(s) | 228 |
| XspI | CTAG | 1 cut(s) | 59 |