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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 245 |
| Acc36I | ACCTGC | 1 cut(s) | 65 |
| AccI | GTMKAC | 2 cut(s) | 187, 307 |
| AclWI | GGATC | 1 cut(s) | 163 |
| AcuI | CTGAAG | 1 cut(s) | 171 |
| AgsI | TTSAA | 1 cut(s) | 182 |
| AjiI | CACGTC | 1 cut(s) | 227 |
| AluBI | AGCT | 2 cut(s) | 220, 251 |
| AluI | AGCT | 2 cut(s) | 220, 251 |
| Alw26I | GTCTC | 1 cut(s) | 42 |
| AlwI | GGATC | 1 cut(s) | 163 |
| AspLEI | GCGC | 1 cut(s) | 246 |
| BccI | CCATC | 3 cut(s) | 17, 190, 323 |
| BcoDI | GTCTC | 1 cut(s) | 42 |
| BfaI | CTAG | 2 cut(s) | 270, 311 |
| BfmI | CTRYAG | 1 cut(s) | 188 |
| BfuAI | ACCTGC | 1 cut(s) | 65 |
| BmgBI | CACGTC | 1 cut(s) | 227 |
| BoxI | GACNNNNGTC | 1 cut(s) | 54 |
| BpuEI | CTTGAG | 2 cut(s) | 237, 251 |
| BsaJI | CCNNGG | 1 cut(s) | 297 |
| Bse1I | ACTGG | 1 cut(s) | 166 |
| BseDI | CCNNGG | 1 cut(s) | 297 |
| BseGI | GGATG | 1 cut(s) | 334 |
| BseMII | CTCAG | 1 cut(s) | 254 |
| BseNI | ACTGG | 1 cut(s) | 166 |
| BsmAI | GTCTC | 1 cut(s) | 42 |
| Bsp143I | GATC | 2 cut(s) | 155, 259 |
| Bsp19I | CCATGG | 1 cut(s) | 297 |
| BspCNI | CTCAG | 1 cut(s) | 255 |
| BspMI | ACCTGC | 1 cut(s) | 65 |
| BspPI | GGATC | 1 cut(s) | 163 |
| BsrI | ACTGG | 1 cut(s) | 166 |
| BssECI | CCNNGG | 1 cut(s) | 297 |
| BssMI | GATC | 2 cut(s) | 155, 259 |
| BssT1I | CCWWGG | 1 cut(s) | 297 |
| BstC8I | GCNNGC | 2 cut(s) | 222, 294 |
| BstDEI | CTNAG | 1 cut(s) | 263 |
| BstDSI | CCRYGG | 1 cut(s) | 297 |
| BstF5I | GGATG | 1 cut(s) | 334 |
| BstHHI | GCGC | 1 cut(s) | 246 |
| BstKTI | GATC | 2 cut(s) | 158, 262 |
| BstMAI | GTCTC | 1 cut(s) | 42 |
| BstMBI | GATC | 2 cut(s) | 155, 259 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 54 |
| BstSFI | CTRYAG | 1 cut(s) | 188 |
| BtgI | CCRYGG | 1 cut(s) | 297 |
| BtrI | CACGTC | 1 cut(s) | 227 |
| BtsCI | GGATG | 1 cut(s) | 334 |
| BveI | ACCTGC | 1 cut(s) | 65 |
| Cac8I | GCNNGC | 2 cut(s) | 222, 294 |
| CfoI | GCGC | 1 cut(s) | 246 |
| CviAII | CATG | 2 cut(s) | 67, 298 |
| CviJI | RGCY | 3 cut(s) | 90, 220, 251 |
| CviKI_1 | RGCY | 3 cut(s) | 90, 220, 251 |
| DdeI | CTNAG | 1 cut(s) | 263 |
| DpnI | GATC | 2 cut(s) | 157, 261 |
| DpnII | GATC | 2 cut(s) | 155, 259 |
| Eco130I | CCWWGG | 1 cut(s) | 297 |
| Eco57I | CTGAAG | 1 cut(s) | 171 |
| EcoT14I | CCWWGG | 1 cut(s) | 297 |
| ErhI | CCWWGG | 1 cut(s) | 297 |
| FaeI | CATG | 2 cut(s) | 70, 301 |
| FaiI | YATR | 5 cut(s) | 68, 138, 140, 171, 299 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 113, 145 |
| FatI | CATG | 2 cut(s) | 66, 297 |
| FblI | GTMKAC | 2 cut(s) | 187, 307 |
| FokI | GGATG | 1 cut(s) | 341 |
| FspBI | CTAG | 2 cut(s) | 270, 311 |
| FspI | TGCGCA | 1 cut(s) | 245 |
| GlaI | GCGC | 1 cut(s) | 245 |
| HhaI | GCGC | 1 cut(s) | 246 |
| Hin1II | CATG | 2 cut(s) | 70, 301 |
| Hin6I | GCGC | 1 cut(s) | 244 |
| HinP1I | GCGC | 1 cut(s) | 244 |
| HinfI | GANTC | 2 cut(s) | 101, 314 |
| Hpy166II | GTNNAC | 2 cut(s) | 188, 308 |
| Hpy188I | TCNGA | 3 cut(s) | 151, 160, 264 |
| Hpy188III | TCNNGA | 3 cut(s) | 59, 230, 254 |
| Hpy8I | GTNNAC | 2 cut(s) | 188, 308 |
| HpyAV | CCTTC | 3 cut(s) | 146, 208, 319 |
| HpyCH4IV | ACGT | 2 cut(s) | 226, 282 |
| HpyCH4V | TGCA | 2 cut(s) | 119, 224 |
| HpyF3I | CTNAG | 1 cut(s) | 263 |
| HpySE526I | ACGT | 2 cut(s) | 226, 282 |
| Hsp92II | CATG | 2 cut(s) | 70, 301 |
| HspAI | GCGC | 1 cut(s) | 244 |
| Kzo9I | GATC | 2 cut(s) | 155, 259 |
| LmnI | GCTCC | 1 cut(s) | 217 |
| LpnPI | CCDG | 7 cut(s) | 26, 60, 67, 107, 176, 179, 306 |
| MaeI | CTAG | 2 cut(s) | 270, 311 |
| MaeII | ACGT | 2 cut(s) | 226, 282 |
| MaeIII | GTNAC | 1 cut(s) | 278 |
| MalI | GATC | 2 cut(s) | 157, 261 |
| MboI | GATC | 2 cut(s) | 155, 259 |
| MboII | GAAGA | 1 cut(s) | 309 |
| MmeI | TCCRAC | 1 cut(s) | 78 |
| MnlI | CCTC | 3 cut(s) | 47, 138, 226 |
| MseI | TTAA | 2 cut(s) | 81, 107 |
| NcoI | CCATGG | 1 cut(s) | 297 |
| NdeII | GATC | 2 cut(s) | 155, 259 |
| NlaIII | CATG | 2 cut(s) | 70, 301 |
| NmuCI | GTSAC | 1 cut(s) | 278 |
| NsbI | TGCGCA | 1 cut(s) | 245 |
| PfeI | GAWTC | 2 cut(s) | 101, 314 |
| PshAI | GACNNNNGTC | 1 cut(s) | 54 |
| SaqAI | TTAA | 2 cut(s) | 81, 107 |
| Sau3AI | GATC | 2 cut(s) | 155, 259 |
| SetI | ASST | 9 cut(s) | 56, 79, 118, 149, 187, 222, 229, 253, 285 |
| SfcI | CTRYAG | 1 cut(s) | 188 |
| SmlI | CTYRAG | 2 cut(s) | 230, 252 |
| SmoI | CTYRAG | 2 cut(s) | 230, 252 |
| SspMI | CTAG | 2 cut(s) | 270, 311 |
| StyI | CCWWGG | 1 cut(s) | 297 |
| TaiI | ACGT | 2 cut(s) | 229, 285 |
| TfiI | GAWTC | 2 cut(s) | 101, 314 |
| Tru1I | TTAA | 2 cut(s) | 81, 107 |
| Tru9I | TTAA | 2 cut(s) | 81, 107 |
| TseFI | GTSAC | 1 cut(s) | 278 |
| Tsp45I | GTSAC | 1 cut(s) | 278 |
| TspDTI | ATGAA | 1 cut(s) | 55 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 20 |
| XmiI | GTMKAC | 2 cut(s) | 187, 307 |
| XspI | CTAG | 2 cut(s) | 270, 311 |