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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 144, 213 |
| AciI | CCGC | 3 cut(s) | 7, 88, 508 |
| AclWI | GGATC | 1 cut(s) | 348 |
| AcuI | CTGAAG | 1 cut(s) | 487 |
| AfaI | GTAC | 2 cut(s) | 43, 570 |
| AfeI | AGCGCT | 1 cut(s) | 460 |
| AflIII | ACRYGT | 1 cut(s) | 163 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 153, 185 |
| AluBI | AGCT | 3 cut(s) | 224, 434, 444 |
| AluI | AGCT | 3 cut(s) | 224, 434, 444 |
| Alw26I | GTCTC | 1 cut(s) | 442 |
| AlwI | GGATC | 1 cut(s) | 348 |
| Ama87I | CYCGRG | 1 cut(s) | 445 |
| Aor51HI | AGCGCT | 1 cut(s) | 460 |
| ApeKI | GCWGC | 3 cut(s) | 4, 82, 434 |
| AspLEI | GCGC | 1 cut(s) | 461 |
| AsuHPI | GGTGA | 1 cut(s) | 254 |
| AvaI | CYCGRG | 1 cut(s) | 445 |
| AxyI | CCTNAGG | 1 cut(s) | 327 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 380, 413 |
| BauI | CACGAG | 1 cut(s) | 516 |
| BbsI | GAAGAC | 1 cut(s) | 493 |
| BbvI | GCAGC | 3 cut(s) | 16, 69, 421 |
| BccI | CCATC | 2 cut(s) | 97, 404 |
| BceAI | ACGGC | 1 cut(s) | 379 |
| BcoDI | GTCTC | 1 cut(s) | 442 |
| BfaI | CTAG | 3 cut(s) | 45, 117, 441 |
| BfmI | CTRYAG | 1 cut(s) | 2 |
| BfoI | RGCGCY | 1 cut(s) | 462 |
| BisI | GCNGC | 3 cut(s) | 5, 83, 435 |
| BlsI | GCNGC | 3 cut(s) | 6, 84, 436 |
| BmeT110I | CYCGRG | 1 cut(s) | 445 |
| BmsI | GCATC | 2 cut(s) | 61, 475 |
| BpiI | GAAGAC | 1 cut(s) | 493 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 131, 163 |
| BpuEI | CTTGAG | 1 cut(s) | 164 |
| BsaI | GGTCTC | 1 cut(s) | 442 |
| BsaJI | CCNNGG | 1 cut(s) | 207 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 502, 532 |
| Bse1I | ACTGG | 2 cut(s) | 40, 356 |
| Bse21I | CCTNAGG | 1 cut(s) | 327 |
| BseDI | CCNNGG | 1 cut(s) | 207 |
| BseGI | GGATG | 3 cut(s) | 52, 205, 345 |
| BseMII | CTCAG | 1 cut(s) | 129 |
| BseNI | ACTGG | 2 cut(s) | 40, 356 |
| BseXI | GCAGC | 3 cut(s) | 16, 69, 421 |
| BsiHKCI | CYCGRG | 1 cut(s) | 445 |
| BsmAI | GTCTC | 1 cut(s) | 442 |
| Bso31I | GGTCTC | 1 cut(s) | 442 |
| BsoBI | CYCGRG | 1 cut(s) | 445 |
| Bsp143I | GATC | 1 cut(s) | 340 |
| BspACI | CCGC | 3 cut(s) | 7, 88, 508 |
| BspCNI | CTCAG | 1 cut(s) | 130 |
| BspMAI | CTGCAG | 1 cut(s) | 6 |
| BspPI | GGATC | 1 cut(s) | 348 |
| BspTNI | GGTCTC | 1 cut(s) | 442 |
| BsrI | ACTGG | 2 cut(s) | 40, 356 |
| BssECI | CCNNGG | 1 cut(s) | 207 |
| BssMI | GATC | 1 cut(s) | 340 |
| BssNAI | GTATAC | 1 cut(s) | 145 |
| BssSI | CACGAG | 1 cut(s) | 516 |
| Bst1107I | GTATAC | 1 cut(s) | 145 |
| Bst2BI | CACGAG | 1 cut(s) | 516 |
| Bst4CI | ACNGT | 3 cut(s) | 100, 496, 552 |
| Bst6I | CTCTTC | 1 cut(s) | 357 |
| BstDEI | CTNAG | 4 cut(s) | 138, 327, 403, 465 |
| BstF5I | GGATG | 3 cut(s) | 52, 205, 345 |
| BstH2I | RGCGCY | 1 cut(s) | 462 |
| BstHHI | GCGC | 1 cut(s) | 461 |
| BstKTI | GATC | 1 cut(s) | 343 |
| BstMAI | GTCTC | 1 cut(s) | 442 |
| BstMBI | GATC | 1 cut(s) | 340 |
| BstSFI | CTRYAG | 1 cut(s) | 2 |
| BstV1I | GCAGC | 3 cut(s) | 16, 69, 421 |
| BstV2I | GAAGAC | 1 cut(s) | 493 |
| BstZ17I | GTATAC | 1 cut(s) | 145 |
| Bsu36I | CCTNAGG | 1 cut(s) | 327 |
| BtsCI | GGATG | 3 cut(s) | 52, 205, 345 |
| BtsIMutI | CAGTG | 1 cut(s) | 135 |
| CfoI | GCGC | 1 cut(s) | 461 |
| Csp6I | GTAC | 2 cut(s) | 42, 569 |
| CviAII | CATG | 3 cut(s) | 112, 482, 529 |
| CviJI | RGCY | 7 cut(s) | 82, 121, 224, 275, 434, 444, 557 |
| CviKI_1 | RGCY | 7 cut(s) | 82, 121, 224, 275, 434, 444, 557 |
| CviQI | GTAC | 2 cut(s) | 42, 569 |
| DdeI | CTNAG | 4 cut(s) | 138, 327, 403, 465 |
| DpnI | GATC | 1 cut(s) | 342 |
| DpnII | GATC | 1 cut(s) | 340 |
| DraI | TTTAAA | 1 cut(s) | 322 |
| Eam1104I | CTCTTC | 1 cut(s) | 357 |
| EarI | CTCTTC | 1 cut(s) | 357 |
| EciI | GGCGGA | 1 cut(s) | 523 |
| Eco31I | GGTCTC | 1 cut(s) | 442 |
| Eco47III | AGCGCT | 1 cut(s) | 460 |
| Eco57I | CTGAAG | 1 cut(s) | 487 |
| Eco81I | CCTNAGG | 1 cut(s) | 327 |
| Eco88I | CYCGRG | 1 cut(s) | 445 |
| FaeI | CATG | 3 cut(s) | 115, 485, 532 |
| FaiI | YATR | 5 cut(s) | 113, 145, 483, 530, 575 |
| FatI | CATG | 3 cut(s) | 111, 481, 528 |
| FblI | GTMKAC | 2 cut(s) | 144, 213 |
| Fnu4HI | GCNGC | 3 cut(s) | 5, 83, 435 |
| FokI | GGATG | 3 cut(s) | 39, 212, 332 |
| Fsp4HI | GCNGC | 3 cut(s) | 5, 83, 435 |
| FspBI | CTAG | 3 cut(s) | 45, 117, 441 |
| GlaI | GCGC | 1 cut(s) | 460 |
| GluI | GCNGC | 3 cut(s) | 5, 83, 435 |
| HaeII | RGCGCY | 1 cut(s) | 462 |
| HhaI | GCGC | 1 cut(s) | 461 |
| Hin1II | CATG | 3 cut(s) | 115, 485, 532 |
| Hin6I | GCGC | 1 cut(s) | 459 |
| HinP1I | GCGC | 1 cut(s) | 459 |
| HinfI | GANTC | 1 cut(s) | 203 |
| HphI | GGTGA | 1 cut(s) | 254 |
| Hpy166II | GTNNAC | 3 cut(s) | 145, 214, 569 |
| Hpy188I | TCNGA | 2 cut(s) | 208, 280 |
| Hpy188III | TCNNGA | 1 cut(s) | 447 |
| Hpy8I | GTNNAC | 3 cut(s) | 145, 214, 569 |
| Hpy99I | CGWCG | 1 cut(s) | 400 |
| HpyAV | CCTTC | 3 cut(s) | 232, 253, 301 |
| HpyCH4III | ACNGT | 3 cut(s) | 100, 496, 552 |
| HpyCH4IV | ACGT | 1 cut(s) | 163 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 52, 254 |
| HpyF3I | CTNAG | 4 cut(s) | 138, 327, 403, 465 |
| HpySE526I | ACGT | 1 cut(s) | 163 |
| Hsp92II | CATG | 3 cut(s) | 115, 485, 532 |
| HspAI | GCGC | 1 cut(s) | 459 |
| Kzo9I | GATC | 1 cut(s) | 340 |
| LmnI | GCTCC | 1 cut(s) | 90 |
| LpnPI | CCDG | 3 cut(s) | 21, 369, 490 |
| Lsp1109I | GCAGC | 3 cut(s) | 16, 69, 421 |
| LweI | GCATC | 2 cut(s) | 61, 475 |
| MaeI | CTAG | 3 cut(s) | 45, 117, 441 |
| MaeII | ACGT | 1 cut(s) | 163 |
| MaeIII | GTNAC | 3 cut(s) | 127, 242, 475 |
| MalI | GATC | 1 cut(s) | 342 |
| MboI | GATC | 1 cut(s) | 340 |
| MboII | GAAGA | 2 cut(s) | 344, 493 |
| MnlI | CCTC | 6 cut(s) | 15, 72, 133, 202, 360, 499 |
| MseI | TTAA | 5 cut(s) | 30, 123, 270, 321, 381 |
| MslI | CAYNNNNRTG | 1 cut(s) | 57 |
| MspA1I | CMGCKG | 1 cut(s) | 7 |
| NdeII | GATC | 1 cut(s) | 340 |
| NlaIII | CATG | 3 cut(s) | 115, 485, 532 |
| NmuCI | GTSAC | 1 cut(s) | 242 |
| PaeR7I | CTCGAG | 1 cut(s) | 445 |
| PfeI | GAWTC | 1 cut(s) | 203 |
| PkrI | GCNGC | 3 cut(s) | 6, 84, 436 |
| PstI | CTGCAG | 1 cut(s) | 6 |
| RsaI | GTAC | 2 cut(s) | 43, 570 |
| RsaNI | GTAC | 2 cut(s) | 42, 569 |
| RseI | CAYNNNNRTG | 1 cut(s) | 57 |
| SaqAI | TTAA | 5 cut(s) | 30, 123, 270, 321, 381 |
| SatI | GCNGC | 3 cut(s) | 5, 83, 435 |
| Sau3AI | GATC | 1 cut(s) | 340 |
| SfaNI | GCATC | 2 cut(s) | 61, 475 |
| SfcI | CTRYAG | 1 cut(s) | 2 |
| Sfr274I | CTCGAG | 1 cut(s) | 445 |
| SlaI | CTCGAG | 1 cut(s) | 445 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 57 |
| SmlI | CTYRAG | 2 cut(s) | 179, 445 |
| SmoI | CTYRAG | 2 cut(s) | 179, 445 |
| SsiI | CCGC | 3 cut(s) | 7, 88, 508 |
| SspMI | CTAG | 3 cut(s) | 45, 117, 441 |
| TaaI | ACNGT | 3 cut(s) | 100, 496, 552 |
| TaiI | ACGT | 1 cut(s) | 166 |
| TaqI | TCGA | 3 cut(s) | 149, 398, 446 |
| TaqII | GACCGA | 1 cut(s) | 466 |
| TfiI | GAWTC | 1 cut(s) | 203 |
| Tru1I | TTAA | 5 cut(s) | 30, 123, 270, 321, 381 |
| Tru9I | TTAA | 5 cut(s) | 30, 123, 270, 321, 381 |
| TscAI | CASTG | 1 cut(s) | 142 |
| TseFI | GTSAC | 1 cut(s) | 242 |
| TseI | GCWGC | 3 cut(s) | 4, 82, 434 |
| Tsp45I | GTSAC | 1 cut(s) | 242 |
| TspRI | CASTG | 1 cut(s) | 142 |
| XhoI | CTCGAG | 1 cut(s) | 445 |
| XmiI | GTMKAC | 2 cut(s) | 144, 213 |
| XspI | CTAG | 3 cut(s) | 45, 117, 441 |