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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 67 |
| AciI | CCGC | 1 cut(s) | 67 |
| AclWI | GGATC | 2 cut(s) | 126, 139 |
| AfaI | GTAC | 1 cut(s) | 173 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 24 |
| AgsI | TTSAA | 1 cut(s) | 47 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 115 |
| AluBI | AGCT | 3 cut(s) | 11, 34, 105 |
| AluI | AGCT | 3 cut(s) | 11, 34, 105 |
| AlwI | GGATC | 2 cut(s) | 126, 139 |
| AoxI | GGCC | 1 cut(s) | 166 |
| AspLEI | GCGC | 1 cut(s) | 67 |
| AsuHPI | GGTGA | 1 cut(s) | 104 |
| AxyI | CCTNAGG | 1 cut(s) | 83 |
| BamHI | GGATCC | 1 cut(s) | 131 |
| BbsI | GAAGAC | 1 cut(s) | 78 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 115 |
| BmiI | GGNNCC | 2 cut(s) | 133, 144 |
| BmrI | ACTGGG | 1 cut(s) | 13 |
| BmsI | GCATC | 1 cut(s) | 49 |
| BmuI | ACTGGG | 1 cut(s) | 13 |
| BpiI | GAAGAC | 1 cut(s) | 78 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 24 |
| Bse1I | ACTGG | 1 cut(s) | 19 |
| Bse21I | CCTNAGG | 1 cut(s) | 83 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 24 |
| BseMII | CTCAG | 1 cut(s) | 120 |
| BseNI | ACTGG | 1 cut(s) | 19 |
| Bsh1236I | CGCG | 1 cut(s) | 67 |
| BshFI | GGCC | 1 cut(s) | 168 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 24 |
| BsnI | GGCC | 1 cut(s) | 168 |
| Bsp1407I | TGTACA | 1 cut(s) | 171 |
| Bsp143I | GATC | 1 cut(s) | 131 |
| BspACI | CCGC | 1 cut(s) | 67 |
| BspANI | GGCC | 1 cut(s) | 168 |
| BspCNI | CTCAG | 1 cut(s) | 119 |
| BspFNI | CGCG | 1 cut(s) | 67 |
| BspLI | GGNNCC | 2 cut(s) | 133, 144 |
| BspPI | GGATC | 2 cut(s) | 126, 139 |
| BsrGI | TGTACA | 1 cut(s) | 171 |
| BsrI | ACTGG | 1 cut(s) | 19 |
| BssMI | GATC | 1 cut(s) | 131 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 120 |
| BstAUI | TGTACA | 1 cut(s) | 171 |
| BstDEI | CTNAG | 2 cut(s) | 83, 106 |
| BstFNI | CGCG | 1 cut(s) | 67 |
| BstHHI | GCGC | 1 cut(s) | 67 |
| BstKTI | GATC | 1 cut(s) | 134 |
| BstMBI | GATC | 1 cut(s) | 131 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 111, 120 |
| BstNSI | RCATGY | 1 cut(s) | 118 |
| BstUI | CGCG | 1 cut(s) | 67 |
| BstV2I | GAAGAC | 1 cut(s) | 78 |
| BstX2I | RGATCY | 1 cut(s) | 131 |
| BstYI | RGATCY | 1 cut(s) | 131 |
| Bsu36I | CCTNAGG | 1 cut(s) | 83 |
| BsuRI | GGCC | 1 cut(s) | 168 |
| CfoI | GCGC | 1 cut(s) | 67 |
| Csp6I | GTAC | 1 cut(s) | 172 |
| CviAII | CATG | 1 cut(s) | 115 |
| CviJI | RGCY | 5 cut(s) | 11, 34, 88, 105, 168 |
| CviKI_1 | RGCY | 5 cut(s) | 11, 34, 88, 105, 168 |
| CviQI | GTAC | 1 cut(s) | 172 |
| DdeI | CTNAG | 2 cut(s) | 83, 106 |
| DpnI | GATC | 1 cut(s) | 133 |
| DpnII | GATC | 1 cut(s) | 131 |
| DriI | GACNNNNNGTC | 1 cut(s) | 115 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 115 |
| Eco81I | CCTNAGG | 1 cut(s) | 83 |
| FaeI | CATG | 1 cut(s) | 118 |
| FaiI | YATR | 3 cut(s) | 14, 116, 125 |
| FatI | CATG | 1 cut(s) | 114 |
| FauI | CCCGC | 1 cut(s) | 60 |
| GlaI | GCGC | 1 cut(s) | 66 |
| HaeIII | GGCC | 1 cut(s) | 168 |
| HhaI | GCGC | 1 cut(s) | 67 |
| Hin1II | CATG | 1 cut(s) | 118 |
| Hin6I | GCGC | 1 cut(s) | 65 |
| HinP1I | GCGC | 1 cut(s) | 65 |
| HindIII | AAGCTT | 1 cut(s) | 32 |
| HphI | GGTGA | 1 cut(s) | 104 |
| Hpy188I | TCNGA | 1 cut(s) | 109 |
| Hpy188III | TCNNGA | 1 cut(s) | 44 |
| HpyCH4V | TGCA | 3 cut(s) | 40, 123, 158 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 111, 120 |
| HpyF3I | CTNAG | 2 cut(s) | 83, 106 |
| Hsp92II | CATG | 1 cut(s) | 118 |
| HspAI | GCGC | 1 cut(s) | 65 |
| Kzo9I | GATC | 1 cut(s) | 131 |
| LpnPI | CCDG | 3 cut(s) | 32, 133, 182 |
| LweI | GCATC | 1 cut(s) | 49 |
| MalI | GATC | 1 cut(s) | 133 |
| MboI | GATC | 1 cut(s) | 131 |
| MboII | GAAGA | 1 cut(s) | 83 |
| MflI | RGATCY | 1 cut(s) | 131 |
| MluCI | AATT | 1 cut(s) | 53 |
| MnlI | CCTC | 4 cut(s) | 18, 55, 145, 156 |
| MvnI | CGCG | 1 cut(s) | 67 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 111, 120 |
| NdeII | GATC | 1 cut(s) | 131 |
| NlaIII | CATG | 1 cut(s) | 118 |
| NlaIV | GGNNCC | 2 cut(s) | 133, 144 |
| NspI | RCATGY | 1 cut(s) | 118 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 160 |
| PspN4I | GGNNCC | 2 cut(s) | 133, 144 |
| PsuI | RGATCY | 1 cut(s) | 131 |
| RsaI | GTAC | 1 cut(s) | 173 |
| RsaNI | GTAC | 1 cut(s) | 172 |
| Sau3AI | GATC | 1 cut(s) | 131 |
| SetI | ASST | 4 cut(s) | 13, 36, 84, 107 |
| SfaNI | GCATC | 1 cut(s) | 49 |
| Sse9I | AATT | 1 cut(s) | 53 |
| SsiI | CCGC | 1 cut(s) | 67 |
| TasI | AATT | 1 cut(s) | 53 |
| TatI | WGTACW | 1 cut(s) | 171 |
| XceI | RCATGY | 1 cut(s) | 118 |