Catalyzes the isomerization of citrate to isocitrate via cis-aconitate
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 572 |
| AarI | CACCTGC | 1 cut(s) | 2134 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 284 |
| Acc36I | ACCTGC | 2 cut(s) | 2134, 3338 |
| Acc65I | GGTACC | 1 cut(s) | 464 |
| AccB1I | GGYRCC | 1 cut(s) | 464 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2852 |
| AccBSI | CCGCTC | 1 cut(s) | 2578 |
| AccI | GTMKAC | 4 cut(s) | 276, 1779, 2232, 3111 |
| AccII | CGCG | 4 cut(s) | 1127, 2151, 2842, 3277 |
| AclWI | GGATC | 8 cut(s) | 239, 370, 1596, 1609, 1832, 2593, 2817, 3196 |
| AcoI | YGGCCR | 2 cut(s) | 3, 2154 |
| AcsI | RAATTY | 3 cut(s) | 438, 1268, 2915 |
| AcuI | CTGAAG | 1 cut(s) | 1863 |
| AdeI | CACNNNGTG | 1 cut(s) | 2699 |
| AfaI | GTAC | 6 cut(s) | 189, 466, 1436, 2446, 2643, 2930 |
| AflII | CTTAAG | 2 cut(s) | 2815, 3104 |
| AflIII | ACRYGT | 1 cut(s) | 786 |
| AhlI | ACTAGT | 2 cut(s) | 1379, 1700 |
| AjnI | CCWGG | 6 cut(s) | 171, 390, 610, 1202, 1473, 2310 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 2344, 2376, 2975, 3007 |
| Alw21I | GWGCWC | 3 cut(s) | 1387, 1889, 2401 |
| Alw26I | GTCTC | 8 cut(s) | 795, 1112, 1189, 1583, 1708, 2074, 2630, 3047 |
| AlwI | GGATC | 8 cut(s) | 239, 370, 1596, 1609, 1832, 2593, 2817, 3196 |
| Ama87I | CYCGRG | 1 cut(s) | 1580 |
| AoxI | GGCC | 9 cut(s) | 3, 771, 1020, 1131, 1221, 1241, 2154, 2222, 2476 |
| ApeKI | GCWGC | 9 cut(s) | 385, 589, 1292, 1366, 1639, 1859, 2066, 2379, 3140 |
| ApoI | RAATTY | 3 cut(s) | 438, 1268, 2915 |
| AseI | ATTAAT | 1 cut(s) | 546 |
| Asp718I | GGTACC | 1 cut(s) | 464 |
| AspA2I | CCTAGG | 1 cut(s) | 3265 |
| AspLEI | GCGC | 3 cut(s) | 2842, 2844, 3277 |
| AspS9I | GGNCC | 6 cut(s) | 771, 1007, 1020, 1131, 2222, 2477 |
| AsuHPI | GGTGA | 9 cut(s) | 117, 749, 761, 1335, 1418, 2115, 2207, 2396, 3314 |
| AsuII | TTCGAA | 1 cut(s) | 426 |
| AvaI | CYCGRG | 1 cut(s) | 1580 |
| AvaII | GGWCC | 1 cut(s) | 1007 |
| AvrII | CCTAGG | 1 cut(s) | 3265 |
| AxyI | CCTNAGG | 2 cut(s) | 1959, 2135 |
| BaeGI | GKGCMC | 1 cut(s) | 2977 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 171, 204 |
| BalI | TGGCCA | 1 cut(s) | 2156 |
| BamHI | GGATCC | 1 cut(s) | 1601 |
| BanI | GGYRCC | 1 cut(s) | 464 |
| BanII | GRGCYC | 2 cut(s) | 2401, 2625 |
| BauI | CACGAG | 1 cut(s) | 159 |
| Bbv12I | GWGCWC | 3 cut(s) | 1387, 1889, 2401 |
| BbvCI | CCTCAGC | 1 cut(s) | 534 |
| BbvI | GCAGC | 9 cut(s) | 372, 601, 1279, 1353, 1651, 1846, 2053, 2366, 3127 |
| BceAI | ACGGC | 3 cut(s) | 2114, 3066, 3348 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 706, 740, 3013, 3047 |
| BciT130I | CCWGG | 6 cut(s) | 173, 392, 612, 1204, 1475, 2312 |
| BciVI | GTATCC | 2 cut(s) | 1988, 3235 |
| BcoDI | GTCTC | 8 cut(s) | 795, 1112, 1189, 1583, 1708, 2074, 2630, 3047 |
| BcuI | ACTAGT | 2 cut(s) | 1379, 1700 |
| BfaI | CTAG | 7 cut(s) | 1352, 1380, 1680, 1701, 1752, 2949, 3266 |
| BfmI | CTRYAG | 7 cut(s) | 273, 587, 825, 968, 1068, 1555, 2355 |
| BfrI | CTTAAG | 2 cut(s) | 2815, 3104 |
| BfuAI | ACCTGC | 2 cut(s) | 2134, 3338 |
| BfuI | GTATCC | 2 cut(s) | 1988, 3235 |
| BglII | AGATCT | 2 cut(s) | 2861, 3341 |
| BlnI | CCTAGG | 1 cut(s) | 3265 |
| BmcAI | AGTACT | 2 cut(s) | 1436, 2643 |
| Bme1390I | CCNGG | 6 cut(s) | 173, 392, 612, 1204, 1475, 2312 |
| Bme18I | GGWCC | 1 cut(s) | 1007 |
| BmeT110I | CYCGRG | 1 cut(s) | 1580 |
| BmgT120I | GGNCC | 6 cut(s) | 771, 1007, 1020, 1131, 2222, 2477 |
| BmiI | GGNNCC | 6 cut(s) | 270, 466, 1132, 1603, 2904, 3010 |
| BmrFI | CCNGG | 6 cut(s) | 173, 392, 612, 1204, 1475, 2312 |
| BmrI | ACTGGG | 3 cut(s) | 645, 2654, 3168 |
| BmsI | GCATC | 9 cut(s) | 211, 1166, 1476, 1498, 1530, 2107, 2760, 3064, 3259 |
| BmuI | ACTGGG | 3 cut(s) | 645, 2654, 3168 |
| BplI | GAGNNNNNCTC | 8 cut(s) | 1130, 1162, 1322, 1354, 1421, 1453, 1766, 1798 |
| BpmI | CTGGAG | 3 cut(s) | 155, 204, 1971 |
| Bpu10I | CCTNAGC | 2 cut(s) | 534, 3144 |
| Bpu14I | TTCGAA | 1 cut(s) | 426 |
| BpuEI | CTTGAG | 3 cut(s) | 922, 1642, 1799 |
| BsaI | GGTCTC | 2 cut(s) | 1112, 2074 |
| BsaJI | CCNNGG | 9 cut(s) | 84, 225, 810, 1125, 1914, 2190, 2392, 2889, 3265 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 2255, 2285, 2388, 2418, 2759, 2789 |
| Bse118I | RCCGGY | 1 cut(s) | 1357 |
| Bse1I | ACTGG | 7 cut(s) | 187, 640, 865, 1101, 1988, 2649, 3174 |
| Bse21I | CCTNAGG | 2 cut(s) | 1959, 2135 |
| Bse3DI | GCAATG | 3 cut(s) | 839, 891, 2835 |
| BseBI | CCWGG | 6 cut(s) | 173, 392, 612, 1204, 1475, 2312 |
| BseDI | CCNNGG | 9 cut(s) | 84, 225, 810, 1125, 1914, 2190, 2392, 2889, 3265 |
| BseMI | GCAATG | 3 cut(s) | 839, 891, 2835 |
| BseNI | ACTGG | 7 cut(s) | 187, 640, 865, 1101, 1988, 2649, 3174 |
| BsePI | GCGCGC | 1 cut(s) | 2840 |
| BseRI | GAGGAG | 8 cut(s) | 158, 245, 1166, 1431, 1974, 2276, 3014, 3065 |
| BseSI | GKGCMC | 1 cut(s) | 2977 |
| BseXI | GCAGC | 9 cut(s) | 372, 601, 1279, 1353, 1651, 1846, 2053, 2366, 3127 |
| BseYI | CCCAGC | 1 cut(s) | 510 |
| BsgI | GTGCAG | 1 cut(s) | 2522 |
| Bsh1236I | CGCG | 4 cut(s) | 1127, 2151, 2842, 3277 |
| BshFI | GGCC | 9 cut(s) | 5, 773, 1022, 1133, 1223, 1243, 2156, 2224, 2478 |
| BshNI | GGYRCC | 1 cut(s) | 464 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1387, 1889, 2401 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1580 |
| BsiSI | CCGG | 2 cut(s) | 1358, 1863 |
| BslFI | GGGAC | 4 cut(s) | 1690, 2623, 2663, 2870 |
| BsmAI | GTCTC | 8 cut(s) | 795, 1112, 1189, 1583, 1708, 2074, 2630, 3047 |
| BsmFI | GGGAC | 4 cut(s) | 1690, 2623, 2663, 2870 |
| BsmI | GAATGC | 1 cut(s) | 371 |
| BsnI | GGCC | 9 cut(s) | 5, 773, 1022, 1133, 1223, 1243, 2156, 2224, 2478 |
| Bso31I | GGTCTC | 2 cut(s) | 1112, 2074 |
| BsoBI | CYCGRG | 1 cut(s) | 1580 |
| Bsp119I | TTCGAA | 1 cut(s) | 426 |
| Bsp1286I | GDGCHC | 5 cut(s) | 1387, 1889, 2401, 2625, 2977 |
| Bsp19I | CCATGG | 2 cut(s) | 225, 810 |
| BspANI | GGCC | 9 cut(s) | 5, 773, 1022, 1133, 1223, 1243, 2156, 2224, 2478 |
| BspFNI | CGCG | 4 cut(s) | 1127, 2151, 2842, 3277 |
| BspHI | TCATGA | 2 cut(s) | 1078, 1162 |
| BspLI | GGNNCC | 6 cut(s) | 270, 466, 1132, 1603, 2904, 3010 |
| BspMAI | CTGCAG | 1 cut(s) | 591 |
| BspMI | ACCTGC | 2 cut(s) | 2134, 3338 |
| BspPI | GGATC | 8 cut(s) | 239, 370, 1596, 1609, 1832, 2593, 2817, 3196 |
| BspT104I | TTCGAA | 1 cut(s) | 426 |
| BspT107I | GGYRCC | 1 cut(s) | 464 |
| BspTI | CTTAAG | 2 cut(s) | 2815, 3104 |
| BspTNI | GGTCTC | 2 cut(s) | 1112, 2074 |
| BsrBI | CCGCTC | 1 cut(s) | 2578 |
| BsrDI | GCAATG | 3 cut(s) | 839, 891, 2835 |
| BsrFI | RCCGGY | 1 cut(s) | 1357 |
| BsrI | ACTGG | 7 cut(s) | 187, 640, 865, 1101, 1988, 2649, 3174 |
| BssAI | RCCGGY | 1 cut(s) | 1357 |
| BssECI | CCNNGG | 9 cut(s) | 84, 225, 810, 1125, 1914, 2190, 2392, 2889, 3265 |
| BssHII | GCGCGC | 1 cut(s) | 2840 |
| BssNAI | GTATAC | 1 cut(s) | 1780 |
| BssSI | CACGAG | 1 cut(s) | 159 |
| BssT1I | CCWWGG | 6 cut(s) | 84, 225, 810, 2190, 2392, 3265 |
| Bst1107I | GTATAC | 1 cut(s) | 1780 |
| Bst2BI | CACGAG | 1 cut(s) | 159 |
| Bst2UI | CCWGG | 6 cut(s) | 173, 392, 612, 1204, 1475, 2312 |
| Bst6I | CTCTTC | 3 cut(s) | 1063, 2051, 2713 |
| BstAFI | CTTAAG | 2 cut(s) | 2815, 3104 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 2117, 3330 |
| BstBI | TTCGAA | 1 cut(s) | 426 |
| BstC8I | GCNNGC | 7 cut(s) | 156, 508, 1402, 1857, 2842, 3079, 3138 |
| BstDSI | CCRYGG | 3 cut(s) | 225, 810, 1125 |
| BstEII | GGTNACC | 2 cut(s) | 1808, 1963 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 3269 |
| BstFNI | CGCG | 4 cut(s) | 1127, 2151, 2842, 3277 |
| BstHHI | GCGC | 3 cut(s) | 2842, 2844, 3277 |
| BstMAI | GTCTC | 8 cut(s) | 795, 1112, 1189, 1583, 1708, 2074, 2630, 3047 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 637, 770, 779, 1363, 1527, 2117, 2126, 3321, 3330 |
| BstNI | CCWGG | 6 cut(s) | 173, 392, 612, 1204, 1475, 2312 |
| BstNSI | RCATGY | 5 cut(s) | 213, 385, 790, 1404, 3081 |
| BstPI | GGTNACC | 2 cut(s) | 1808, 1963 |
| BstSCI | CCNGG | 6 cut(s) | 171, 390, 610, 1202, 1473, 2310 |
| BstSFI | CTRYAG | 7 cut(s) | 273, 587, 825, 968, 1068, 1555, 2355 |
| BstSLI | GKGCMC | 1 cut(s) | 2977 |
| BstUI | CGCG | 4 cut(s) | 1127, 2151, 2842, 3277 |
| BstV1I | GCAGC | 9 cut(s) | 372, 601, 1279, 1353, 1651, 1846, 2053, 2366, 3127 |
| BstX2I | RGATCY | 5 cut(s) | 362, 1601, 2861, 3201, 3341 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 601 |
| BstYI | RGATCY | 5 cut(s) | 362, 1601, 2861, 3201, 3341 |
| BstZ17I | GTATAC | 1 cut(s) | 1780 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1959, 2135 |
| BsuI | GTATCC | 2 cut(s) | 1988, 3235 |
| BsuRI | GGCC | 9 cut(s) | 5, 773, 1022, 1133, 1223, 1243, 2156, 2224, 2478 |
| BtgI | CCRYGG | 3 cut(s) | 225, 810, 1125 |
| BtgZI | GCGATG | 2 cut(s) | 1470, 2313 |
| BtsI | GCAGTG | 2 cut(s) | 584, 657 |
| BtsIMutI | CAGTG | 7 cut(s) | 9, 180, 584, 657, 1094, 1731, 3181 |
| BveI | ACCTGC | 2 cut(s) | 2134, 3338 |
| Cac8I | GCNNGC | 7 cut(s) | 156, 508, 1402, 1857, 2842, 3079, 3138 |
| CciI | TCATGA | 2 cut(s) | 1078, 1162 |
| CfoI | GCGC | 3 cut(s) | 2842, 2844, 3277 |
| Cfr10I | RCCGGY | 1 cut(s) | 1357 |
| Cfr13I | GGNCC | 6 cut(s) | 771, 1007, 1020, 1131, 2222, 2477 |
| Cfr42I | CCGCGG | 1 cut(s) | 1128 |
| CseI | GACGC | 1 cut(s) | 1676 |
| CsiI | ACCWGGT | 1 cut(s) | 610 |
| Csp6I | GTAC | 6 cut(s) | 188, 465, 1435, 2445, 2642, 2929 |
| CviQI | GTAC | 6 cut(s) | 188, 465, 1435, 2445, 2642, 2929 |
| DraIII | CACNNNGTG | 1 cut(s) | 2699 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 284 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 284 |
| EaeI | YGGCCR | 2 cut(s) | 3, 2154 |
| Eam1104I | CTCTTC | 3 cut(s) | 1063, 2051, 2713 |
| EarI | CTCTTC | 3 cut(s) | 1063, 2051, 2713 |
| EciI | GGCGGA | 2 cut(s) | 2155, 2598 |
| Ecl136II | GAGCTC | 1 cut(s) | 2399 |
| Eco130I | CCWWGG | 6 cut(s) | 84, 225, 810, 2190, 2392, 3265 |
| Eco147I | AGGCCT | 1 cut(s) | 1243 |
| Eco24I | GRGCYC | 2 cut(s) | 2401, 2625 |
| Eco31I | GGTCTC | 2 cut(s) | 1112, 2074 |
| Eco47I | GGWCC | 1 cut(s) | 1007 |
| Eco53kI | GAGCTC | 1 cut(s) | 2399 |
| Eco57I | CTGAAG | 1 cut(s) | 1863 |
| Eco81I | CCTNAGG | 2 cut(s) | 1959, 2135 |
| Eco88I | CYCGRG | 1 cut(s) | 1580 |
| Eco91I | GGTNACC | 2 cut(s) | 1808, 1963 |
| EcoICRI | GAGCTC | 1 cut(s) | 2399 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 3269 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1020, 1131 |
| EcoO65I | GGTNACC | 2 cut(s) | 1808, 1963 |
| EcoRI | GAATTC | 1 cut(s) | 438 |
| EcoRII | CCWGG | 6 cut(s) | 171, 390, 610, 1202, 1473, 2310 |
| EcoT14I | CCWWGG | 6 cut(s) | 84, 225, 810, 2190, 2392, 3265 |
| EcoT22I | ATGCAT | 1 cut(s) | 3079 |
| EcoT38I | GRGCYC | 2 cut(s) | 2401, 2625 |
| ErhI | CCWWGG | 6 cut(s) | 84, 225, 810, 2190, 2392, 3265 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 3098, 3130, 3137, 3169, 3331, 3363 |
| FaqI | GGGAC | 4 cut(s) | 1690, 2623, 2663, 2870 |
| FauI | CCCGC | 3 cut(s) | 1120, 2156, 2571 |
| FblI | GTMKAC | 4 cut(s) | 276, 1779, 2232, 3111 |
| FriOI | GRGCYC | 2 cut(s) | 2401, 2625 |
| FspBI | CTAG | 7 cut(s) | 1352, 1380, 1680, 1701, 1752, 2949, 3266 |
| GlaI | GCGC | 3 cut(s) | 2841, 2843, 3276 |
| GsaI | CCCAGC | 1 cut(s) | 514 |
| GsuI | CTGGAG | 3 cut(s) | 155, 204, 1971 |
| HaeIII | GGCC | 9 cut(s) | 5, 773, 1022, 1133, 1223, 1243, 2156, 2224, 2478 |
| HapII | CCGG | 2 cut(s) | 1358, 1863 |
| HgaI | GACGC | 1 cut(s) | 1676 |
| HhaI | GCGC | 3 cut(s) | 2842, 2844, 3277 |
| Hin6I | GCGC | 3 cut(s) | 2840, 2842, 3275 |
| HinP1I | GCGC | 3 cut(s) | 2840, 2842, 3275 |
| HincII | GTYRAC | 2 cut(s) | 2233, 3112 |
| HindII | GTYRAC | 2 cut(s) | 2233, 3112 |
| HindIII | AAGCTT | 3 cut(s) | 629, 3242, 3296 |
| HinfI | GANTC | 8 cut(s) | 47, 470, 893, 1055, 2270, 2318, 2459, 2534 |
| HpaII | CCGG | 2 cut(s) | 1358, 1863 |
| HphI | GGTGA | 9 cut(s) | 117, 749, 761, 1335, 1418, 2115, 2207, 2396, 3314 |
| Hpy166II | GTNNAC | 8 cut(s) | 277, 563, 1505, 1780, 2174, 2180, 2233, 3112 |
| Hpy8I | GTNNAC | 8 cut(s) | 277, 563, 1505, 1780, 2174, 2180, 2233, 3112 |
| Hpy99I | CGWCG | 2 cut(s) | 2188, 2237 |
| HpyCH4IV | ACGT | 1 cut(s) | 2176 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 637, 770, 779, 1363, 1527, 2117, 2126, 3321, 3330 |
| HpySE526I | ACGT | 1 cut(s) | 2176 |
| HspAI | GCGC | 3 cut(s) | 2840, 2842, 3275 |
| KpnI | GGTACC | 1 cut(s) | 468 |
| KspI | CCGCGG | 1 cut(s) | 1128 |
| LmnI | GCTCC | 5 cut(s) | 232, 393, 803, 2396, 2908 |
| Lsp1109I | GCAGC | 9 cut(s) | 372, 601, 1279, 1353, 1651, 1846, 2053, 2366, 3127 |
| LweI | GCATC | 9 cut(s) | 211, 1166, 1476, 1498, 1530, 2107, 2760, 3064, 3259 |
| MabI | ACCWGGT | 1 cut(s) | 610 |
| MaeI | CTAG | 7 cut(s) | 1352, 1380, 1680, 1701, 1752, 2949, 3266 |
| MaeII | ACGT | 1 cut(s) | 2176 |
| MbiI | CCGCTC | 1 cut(s) | 2578 |
| MfeI | CAATTG | 1 cut(s) | 777 |
| MflI | RGATCY | 5 cut(s) | 362, 1601, 2861, 3201, 3341 |
| MhlI | GDGCHC | 5 cut(s) | 1387, 1889, 2401, 2625, 2977 |
| MlsI | TGGCCA | 1 cut(s) | 2156 |
| MluNI | TGGCCA | 1 cut(s) | 2156 |
| MlyI | GAGTC | 3 cut(s) | 1049, 2279, 2327 |
| MmeI | TCCRAC | 3 cut(s) | 1579, 2375, 2584 |
| Mox20I | TGGCCA | 1 cut(s) | 2156 |
| Mph1103I | ATGCAT | 1 cut(s) | 3079 |
| MscI | TGGCCA | 1 cut(s) | 2156 |
| MslI | CAYNNNNRTG | 5 cut(s) | 63, 482, 599, 1736, 2406 |
| Msp20I | TGGCCA | 1 cut(s) | 2156 |
| MspA1I | CMGCKG | 4 cut(s) | 194, 1127, 1642, 1859 |
| MspCI | CTTAAG | 2 cut(s) | 2815, 3104 |
| MspI | CCGG | 2 cut(s) | 1358, 1863 |
| MspR9I | CCNGG | 6 cut(s) | 173, 392, 612, 1204, 1475, 2312 |
| MunI | CAATTG | 1 cut(s) | 777 |
| Mva1269I | GAATGC | 1 cut(s) | 371 |
| MvaI | CCWGG | 6 cut(s) | 173, 392, 612, 1204, 1475, 2312 |
| MvnI | CGCG | 4 cut(s) | 1127, 2151, 2842, 3277 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 637, 770, 779, 1363, 1527, 2117, 2126, 3321, 3330 |
| NcoI | CCATGG | 2 cut(s) | 225, 810 |
| NlaIV | GGNNCC | 6 cut(s) | 270, 466, 1132, 1603, 2904, 3010 |
| NmuCI | GTSAC | 4 cut(s) | 65, 655, 682, 3083 |
| NsiI | ATGCAT | 1 cut(s) | 3079 |
| NspI | RCATGY | 5 cut(s) | 213, 385, 790, 1404, 3081 |
| NspV | TTCGAA | 1 cut(s) | 426 |
| PaeI | GCATGC | 2 cut(s) | 1404, 3081 |
| PagI | TCATGA | 2 cut(s) | 1078, 1162 |
| PaqCI | CACCTGC | 1 cut(s) | 2134 |
| PauI | GCGCGC | 1 cut(s) | 2840 |
| PceI | AGGCCT | 1 cut(s) | 1243 |
| PciI | ACATGT | 1 cut(s) | 786 |
| PctI | GAATGC | 1 cut(s) | 371 |
| PfeI | GAWTC | 5 cut(s) | 47, 470, 893, 2459, 2534 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2852 |
| PfoI | TCCNGGA | 1 cut(s) | 171 |
| PleI | GAGTC | 3 cut(s) | 1049, 2278, 2326 |
| PpsI | GAGTC | 3 cut(s) | 1049, 2278, 2326 |
| PscI | ACATGT | 1 cut(s) | 786 |
| PshBI | ATTAAT | 1 cut(s) | 546 |
| PsiI | TTATAA | 1 cut(s) | 572 |
| Psp124BI | GAGCTC | 1 cut(s) | 2401 |
| Psp6I | CCWGG | 6 cut(s) | 171, 390, 610, 1202, 1473, 2310 |
| PspEI | GGTNACC | 2 cut(s) | 1808, 1963 |
| PspFI | CCCAGC | 1 cut(s) | 510 |
| PspGI | CCWGG | 6 cut(s) | 171, 390, 610, 1202, 1473, 2310 |
| PspN4I | GGNNCC | 6 cut(s) | 270, 466, 1132, 1603, 2904, 3010 |
| PspPI | GGNCC | 6 cut(s) | 771, 1007, 1020, 1131, 2222, 2477 |
| PstI | CTGCAG | 1 cut(s) | 591 |
| PsuI | RGATCY | 5 cut(s) | 362, 1601, 2861, 3201, 3341 |
| PteI | GCGCGC | 1 cut(s) | 2840 |
| PvuII | CAGCTG | 2 cut(s) | 194, 1859 |
| RsaI | GTAC | 6 cut(s) | 189, 466, 1436, 2446, 2643, 2930 |
| RsaNI | GTAC | 6 cut(s) | 188, 465, 1435, 2445, 2642, 2929 |
| RseI | CAYNNNNRTG | 5 cut(s) | 63, 482, 599, 1736, 2406 |
| SacI | GAGCTC | 1 cut(s) | 2401 |
| SacII | CCGCGG | 1 cut(s) | 1128 |
| SalI | GTCGAC | 2 cut(s) | 2231, 3110 |
| Sau96I | GGNCC | 6 cut(s) | 771, 1007, 1020, 1131, 2222, 2477 |
| ScaI | AGTACT | 2 cut(s) | 1436, 2643 |
| SchI | GAGTC | 3 cut(s) | 1049, 2279, 2327 |
| ScrFI | CCNGG | 6 cut(s) | 173, 392, 612, 1204, 1475, 2312 |
| SduI | GDGCHC | 5 cut(s) | 1387, 1889, 2401, 2625, 2977 |
| SexAI | ACCWGGT | 1 cut(s) | 610 |
| SfaNI | GCATC | 9 cut(s) | 211, 1166, 1476, 1498, 1530, 2107, 2760, 3064, 3259 |
| SfcI | CTRYAG | 7 cut(s) | 273, 587, 825, 968, 1068, 1555, 2355 |
| Sfr303I | CCGCGG | 1 cut(s) | 1128 |
| SfuI | TTCGAA | 1 cut(s) | 426 |
| SgrBI | CCGCGG | 1 cut(s) | 1128 |
| SinI | GGWCC | 1 cut(s) | 1007 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 63, 482, 599, 1736, 2406 |
| SmlI | CTYRAG | 5 cut(s) | 901, 1621, 1814, 2815, 3104 |
| SmoI | CTYRAG | 5 cut(s) | 901, 1621, 1814, 2815, 3104 |
| SpeI | ACTAGT | 2 cut(s) | 1379, 1700 |
| SphI | GCATGC | 2 cut(s) | 1404, 3081 |
| SseBI | AGGCCT | 1 cut(s) | 1243 |
| SspMI | CTAG | 7 cut(s) | 1352, 1380, 1680, 1701, 1752, 2949, 3266 |
| SstI | GAGCTC | 1 cut(s) | 2401 |
| StuI | AGGCCT | 1 cut(s) | 1243 |
| StyD4I | CCNGG | 6 cut(s) | 171, 390, 610, 1202, 1473, 2310 |
| StyI | CCWWGG | 6 cut(s) | 84, 225, 810, 2190, 2392, 3265 |
| TaiI | ACGT | 1 cut(s) | 2179 |
| TatI | WGTACW | 2 cut(s) | 1434, 2641 |
| TauI | GCSGC | 1 cut(s) | 8 |
| TfiI | GAWTC | 5 cut(s) | 47, 470, 893, 2459, 2534 |
| TscAI | CASTG | 7 cut(s) | 16, 187, 591, 664, 1101, 1738, 3181 |
| TseFI | GTSAC | 4 cut(s) | 65, 655, 682, 3083 |
| TseI | GCWGC | 9 cut(s) | 385, 589, 1292, 1366, 1639, 1859, 2066, 2379, 3140 |
| Tsp45I | GTSAC | 4 cut(s) | 65, 655, 682, 3083 |
| TspDTI | ATGAA | 6 cut(s) | 568, 910, 1067, 2177, 2670, 3106 |
| TspGWI | ACGGA | 2 cut(s) | 1030, 3001 |
| TspRI | CASTG | 7 cut(s) | 16, 187, 591, 664, 1101, 1738, 3181 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2852 |
| Vha464I | CTTAAG | 2 cut(s) | 2815, 3104 |
| VpaK11BI | GGWCC | 1 cut(s) | 1007 |
| VspI | ATTAAT | 1 cut(s) | 546 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 3269 |
| XapI | RAATTY | 3 cut(s) | 438, 1268, 2915 |
| XbaI | TCTAGA | 1 cut(s) | 1751 |
| XceI | RCATGY | 5 cut(s) | 213, 385, 790, 1404, 3081 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 179 |
| XmaJI | CCTAGG | 1 cut(s) | 3265 |
| XmiI | GTMKAC | 4 cut(s) | 276, 1779, 2232, 3111 |
| XspI | CTAG | 7 cut(s) | 1352, 1380, 1680, 1701, 1752, 2949, 3266 |
| ZrmI | AGTACT | 2 cut(s) | 1436, 2643 |
| Zsp2I | ATGCAT | 1 cut(s) | 3079 |