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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 269 |
| AccBSI | CCGCTC | 1 cut(s) | 78 |
| AccII | CGCG | 1 cut(s) | 300 |
| AciI | CCGC | 2 cut(s) | 42, 78 |
| AclI | AACGTT | 1 cut(s) | 108 |
| AclWI | GGATC | 2 cut(s) | 187, 278 |
| AgsI | TTSAA | 1 cut(s) | 206 |
| AjiI | CACGTC | 1 cut(s) | 251 |
| AluBI | AGCT | 1 cut(s) | 244 |
| AluI | AGCT | 1 cut(s) | 244 |
| Alw26I | GTCTC | 1 cut(s) | 75 |
| AlwI | GGATC | 2 cut(s) | 187, 278 |
| AspLEI | GCGC | 1 cut(s) | 270 |
| AspS9I | GGNCC | 1 cut(s) | 171 |
| AvaII | GGWCC | 1 cut(s) | 171 |
| BccI | CCATC | 1 cut(s) | 214 |
| BcoDI | GTCTC | 1 cut(s) | 75 |
| BfmI | CTRYAG | 1 cut(s) | 212 |
| Bme18I | GGWCC | 1 cut(s) | 171 |
| BmgBI | CACGTC | 1 cut(s) | 251 |
| BmgT120I | GGNCC | 1 cut(s) | 171 |
| BpuEI | CTTGAG | 2 cut(s) | 261, 275 |
| BsaJI | CCNNGG | 1 cut(s) | 319 |
| Bse118I | RCCGGY | 2 cut(s) | 67, 98 |
| BseDI | CCNNGG | 1 cut(s) | 319 |
| BseRI | GAGGAG | 1 cut(s) | 50 |
| Bsh1236I | CGCG | 1 cut(s) | 300 |
| Bsh1285I | CGRYCG | 1 cut(s) | 298 |
| BsiEI | CGRYCG | 1 cut(s) | 298 |
| BsiSI | CCGG | 2 cut(s) | 68, 99 |
| BsmAI | GTCTC | 1 cut(s) | 75 |
| Bsp143I | GATC | 3 cut(s) | 179, 283, 295 |
| Bsp19I | CCATGG | 1 cut(s) | 319 |
| BspACI | CCGC | 2 cut(s) | 42, 78 |
| BspFNI | CGCG | 1 cut(s) | 300 |
| BspMAI | CTGCAG | 1 cut(s) | 216 |
| BspPI | GGATC | 2 cut(s) | 187, 278 |
| BsrBI | CCGCTC | 1 cut(s) | 78 |
| BsrFI | RCCGGY | 2 cut(s) | 67, 98 |
| BssAI | RCCGGY | 2 cut(s) | 67, 98 |
| BssECI | CCNNGG | 1 cut(s) | 319 |
| BssMI | GATC | 3 cut(s) | 179, 283, 295 |
| BssT1I | CCWWGG | 1 cut(s) | 319 |
| BstC8I | GCNNGC | 2 cut(s) | 246, 316 |
| BstDSI | CCRYGG | 1 cut(s) | 319 |
| BstFNI | CGCG | 1 cut(s) | 300 |
| BstHHI | GCGC | 1 cut(s) | 270 |
| BstKTI | GATC | 3 cut(s) | 182, 286, 298 |
| BstMAI | GTCTC | 1 cut(s) | 75 |
| BstMBI | GATC | 3 cut(s) | 179, 283, 295 |
| BstMCI | CGRYCG | 1 cut(s) | 298 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 95 |
| BstSFI | CTRYAG | 1 cut(s) | 212 |
| BstUI | CGCG | 1 cut(s) | 300 |
| BtgI | CCRYGG | 1 cut(s) | 319 |
| BtgZI | GCGATG | 1 cut(s) | 70 |
| BtrI | CACGTC | 1 cut(s) | 251 |
| Cac8I | GCNNGC | 2 cut(s) | 246, 316 |
| CfoI | GCGC | 1 cut(s) | 270 |
| Cfr10I | RCCGGY | 2 cut(s) | 67, 98 |
| Cfr13I | GGNCC | 1 cut(s) | 171 |
| CviAII | CATG | 3 cut(s) | 13, 52, 320 |
| CviJI | RGCY | 4 cut(s) | 56, 114, 244, 275 |
| CviKI_1 | RGCY | 4 cut(s) | 56, 114, 244, 275 |
| DpnI | GATC | 3 cut(s) | 181, 285, 297 |
| DpnII | GATC | 3 cut(s) | 179, 283, 295 |
| Eco130I | CCWWGG | 1 cut(s) | 319 |
| Eco47I | GGWCC | 1 cut(s) | 171 |
| EcoT14I | CCWWGG | 1 cut(s) | 319 |
| ErhI | CCWWGG | 1 cut(s) | 319 |
| FaeI | CATG | 3 cut(s) | 16, 55, 323 |
| FaiI | YATR | 5 cut(s) | 14, 53, 162, 164, 321 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 137, 169 |
| FatI | CATG | 3 cut(s) | 12, 51, 319 |
| FspI | TGCGCA | 1 cut(s) | 269 |
| GlaI | GCGC | 1 cut(s) | 269 |
| HapII | CCGG | 2 cut(s) | 68, 99 |
| HhaI | GCGC | 1 cut(s) | 270 |
| Hin1II | CATG | 3 cut(s) | 16, 55, 323 |
| Hin6I | GCGC | 1 cut(s) | 268 |
| HinP1I | GCGC | 1 cut(s) | 268 |
| HinfI | GANTC | 2 cut(s) | 38, 125 |
| HpaII | CCGG | 2 cut(s) | 68, 99 |
| Hpy188I | TCNGA | 3 cut(s) | 175, 288, 295 |
| Hpy188III | TCNNGA | 2 cut(s) | 254, 278 |
| HpyAV | CCTTC | 2 cut(s) | 170, 232 |
| HpyCH4IV | ACGT | 3 cut(s) | 108, 195, 250 |
| HpyCH4V | TGCA | 3 cut(s) | 143, 214, 248 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 95 |
| HpySE526I | ACGT | 3 cut(s) | 108, 195, 250 |
| Hsp92II | CATG | 3 cut(s) | 16, 55, 323 |
| HspAI | GCGC | 1 cut(s) | 268 |
| Kzo9I | GATC | 3 cut(s) | 179, 283, 295 |
| LmnI | GCTCC | 1 cut(s) | 241 |
| LpnPI | CCDG | 4 cut(s) | 81, 112, 200, 328 |
| MaeII | ACGT | 3 cut(s) | 108, 195, 250 |
| MaeIII | GTNAC | 2 cut(s) | 61, 301 |
| MalI | GATC | 3 cut(s) | 181, 285, 297 |
| MbiI | CCGCTC | 1 cut(s) | 78 |
| MboI | GATC | 3 cut(s) | 179, 283, 295 |
| MluCI | AATT | 1 cut(s) | 337 |
| MlyI | GAGTC | 1 cut(s) | 47 |
| MmeI | TCCRAC | 1 cut(s) | 102 |
| MnlI | CCTC | 3 cut(s) | 28, 162, 250 |
| MseI | TTAA | 5 cut(s) | 47, 105, 131, 198, 336 |
| MspI | CCGG | 2 cut(s) | 68, 99 |
| MvnI | CGCG | 1 cut(s) | 300 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 95 |
| NcoI | CCATGG | 1 cut(s) | 319 |
| NdeII | GATC | 3 cut(s) | 179, 283, 295 |
| NlaIII | CATG | 3 cut(s) | 16, 55, 323 |
| NmuCI | GTSAC | 1 cut(s) | 301 |
| NsbI | TGCGCA | 1 cut(s) | 269 |
| PfeI | GAWTC | 1 cut(s) | 125 |
| Ple19I | CGATCG | 1 cut(s) | 298 |
| PleI | GAGTC | 1 cut(s) | 46 |
| PpsI | GAGTC | 1 cut(s) | 46 |
| Psp1406I | AACGTT | 1 cut(s) | 108 |
| PspPI | GGNCC | 1 cut(s) | 171 |
| PstI | CTGCAG | 1 cut(s) | 216 |
| PvuI | CGATCG | 1 cut(s) | 298 |
| SaqAI | TTAA | 5 cut(s) | 47, 105, 131, 198, 336 |
| Sau3AI | GATC | 3 cut(s) | 179, 283, 295 |
| Sau96I | GGNCC | 1 cut(s) | 171 |
| SchI | GAGTC | 1 cut(s) | 47 |
| SetI | ASST | 7 cut(s) | 111, 142, 173, 198, 211, 246, 253 |
| SfcI | CTRYAG | 1 cut(s) | 212 |
| SgrAI | CRCCGGYG | 1 cut(s) | 67 |
| SinI | GGWCC | 1 cut(s) | 171 |
| SmlI | CTYRAG | 2 cut(s) | 254, 276 |
| SmoI | CTYRAG | 2 cut(s) | 254, 276 |
| Sse9I | AATT | 1 cut(s) | 337 |
| SsiI | CCGC | 2 cut(s) | 42, 78 |
| StyI | CCWWGG | 1 cut(s) | 319 |
| TaiI | ACGT | 3 cut(s) | 111, 198, 253 |
| TaqI | TCGA | 1 cut(s) | 19 |
| TasI | AATT | 1 cut(s) | 337 |
| TfiI | GAWTC | 1 cut(s) | 125 |
| Tru1I | TTAA | 5 cut(s) | 47, 105, 131, 198, 336 |
| Tru9I | TTAA | 5 cut(s) | 47, 105, 131, 198, 336 |
| TseFI | GTSAC | 1 cut(s) | 301 |
| Tsp45I | GTSAC | 1 cut(s) | 301 |
| VpaK11BI | GGWCC | 1 cut(s) | 171 |