Rh7DG483800

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000153
Physical Location & Seq
Reverse (-)
68949329 .. 68963351
14023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG483800.1

Sequence Viewer

Length: 2304 bp
ATGGCTCGAGCATATGAGTCTTCTCAGCCCATTGAGCTTCGTGACCACCACTTCTCACTGGAGGATGGTCTCATTCCTACTTCTACCGCGGGGGATTGTGAAGGTAGCTCGGATGAGCCATTTCGTGAATTTTCTGAGCGTTTTGTTTCTTCCAAGTCCATATCCAGATGGTTCAGAAGGAAAGCCAAGAAGGGTGCATTTAAGCCTGATGTCCTAGATGACATTGGACAATTCAATTTCAGGCCGTATTTTACTGTTCACGGCTTCCAGACATTTCCTTTCTTTTATCCAGAGGCTAACTCATCACCATTCTTGGAATCCGTTGAGTTGCTTCTAGCCACTAGTGCGCAGTCCATAATAGCATATGGTGATTTTTTTGACCCTGCGATGGAGATGTATTCCCCCCATAGAGTTAAGAGGCAATTTGGCATCGATCAGGATGTGCCATCGCATCTATCCTACGGTGGACATCTTGGGAATTGCATTATGGCTTTCACTCGGACAGATGGTATCTCTTGCAGCGGTATACTTTCTGATAGTCTTGGGAAGAAGGATAGTGTCGGTTCTCCTACATTAGCATTCTTGGAATTTTGGGAGCTGCAGCTGTGCGAGTTCAAGAGGTTTATTAAGACTGGTAGTACTAGGTTGTGTCCGAAAGTTAGTAGTGAGAACAAGAGGCTGCTCCGCAGTAGCACTGTCATGGGTGTGATGTCTAGACTGAACCTTCCCTATACCGAGGTATACTCGAATGGTATAGAGCGAGTGGTTGGTCATCTTAAAGGTGGGAATGATTCTGTGGCGGACTTGATTACCACAAGAATAGAGAAGGGTGACAAGTCTGTGGTAAAAGGCAAAGTCAGAACTACTAAGAATGGAGAAGAGAGTAAAAGGAAGGTTCACCGCTTCAAACCTAAGGTGGCAAAGGACCGCATTCCTCCCCTGGTTGTCAAAAGCGCCTTTGGTGTTAGAGTGATACTACCGTCGGATTTGGTTGATGGAAAAGGGAAGAAGAGGAAAACTGCTTCTAGCTCAAAACAGAGTAGAGCTACTAAGCTAAAGGTTACACGTGATGCCTCACCCACTGTCCTCAGGCGAAGTTCCCGCGTTGCCAACATGAGTTTTTCAAAACGTAAACCTTCGTCTAAGGGCGCTGTTTATGTTGATCTTAGTGATGCGCCAAGTGACGACAGCGAGGGTAGCCCCCTTAGTGATAATGTTGACACATATGAGGAGAATGATTCTGACACTTATGAGGAAAGGGTCTGTGATGATGGTAACCAGCTGGAATCCCTTGAAGATGGGGGTAATGACGATTGGGGTAATCGAAACGTGACTTTGAATGAGGGGGATCACTGCAGTGATAACCCAGACATTGGCACTGATGATTTGGGGAAAGAATCTGTTGCAGATGGTAGCCTTTGCGCGGGTGACCATAATGAGCCGGGTGGATCAGTTTTGTTGACAGTTGGAAGGACATCGTTTGAGCCCCACGTCCCTAAGACAGTACCAAGGGATAATGGTGTTGCTGCTGTAATCTTGACCAATAGAGGGATTCCACTTTGTGCTACCTCCGATTGCTCTCTTGCTCCTAACTCAGATAAATCAGAAAAGGTGTCAAAAGCTGATTTGGAGATGCGTTTTAGGAAATTTACTTCTTCTTTTGACACTGAGGATCGCTTGAGTATTACTATTGATGATATTGCACGCCCATTGAGTAGTACAAGAAAGCAAGATGAGAGTGCTTCTTCATTTATTTGCTGGAAATATTACAAACTTGTAACATCACCATGGTGGCGTATTTACAAAAACTCGGAGATCGAAACACAAGTAAATGGAACACAGGACAGGGCTTTCTCTGGAATACTTCCATTTCATGTTGAGTTAAGTGAAGCACTTGAAACCCTCAAGGGAAGAAACCAAGATGCATTATTGGCTGCTTGCCTACACATTGCTTGTAAACAAGAAGACAAGCCCAGAACTCTCAAGGAAATCTTCTCCGTCGCGAATGGAGCCTCAAAGAAAGAGATTGGTAGAGCAAAGGAATACATAATCAAGCAACTGGGGTTAATGGAGAAGCCGTTACAAGTAGCAAATGCTAGGGACTATATGAGGCGATTCTGTTCCTCTCTTGGCTTGAACAATCAAGCAATCAAAGCAGCACAACAAGCTGTGCACAAGTCGCAAGAATTTGATATAAGGAGGAGCCCTGTATCGATAGCCGCAGCAGTGATTTGCATCATCACTCAGCTTTCAGATGTAGTAACAAGAAGCCTCTCAAAGATATCGCAGACGTTACAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

767

Amino Acids

84.99

Weight (kDa)

8.2

Isoelectric Point (pI)

47.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 54 - 152 2.4e-06 Plant mobile domain
TFIIB PF00382 632 - 677 2.7e-08 Transcription factor TFIIB repeat
TFIIB PF00382 695 - 766 1.5e-06 Transcription factor TFIIB repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000153)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25700 FvH4_2g11190 FvH4_2g11201 FvH4_2g16130 FvH4_3g40630 FvH4_4g02210 FvH4_4g14564 FvH4_6g24041 FvH4_6g24050 FvH4_6g24050
rosa_chinensis RchiOBHm_Chr1g0348241 RchiOBHm_Chr2g0109211 RchiOBHm_Chr2g0144471 RchiOBHm_Chr2g0174771 RchiOBHm_Chr3g0495101 RchiOBHm_Chr5g0047301 RchiOBHm_Chr5g0062201
rosa_laevigata RLG00000000570 RLG00000000571 RLG00000008718 RLG00000008719 RLG00000008835 RLG00000019314 RLG00000019316 RLG00000020979
rosa_multiflora Rmu_co8024416.1_g000001 Rmu_co8152282.1_g000001 Rmu_sc0000103.1_g000058 Rmu_sc0000161.1_g000022 Rmu_sc0000517.1_g000009 Rmu_sc0000517.1_g000010 Rmu_sc0000571.1_g000024 Rmu_sc0000580.1_g000130 Rmu_sc0000744.1_g000130 Rmu_sc0000796.1_g000017 Rmu_sc0000800.1_g000021 Rmu_sc0000830.1_g000037 Rmu_sc0000830.1_g000038 Rmu_sc0000830.1_g000059 Rmu_sc0000958.1_g000012 Rmu_sc0001094.1_g000018 Rmu_sc0001163.1_g000004 Rmu_sc0001200.1_g000052 Rmu_sc0001339.1_g000005 Rmu_sc0001397.1_g000009 Rmu_sc0001563.1_g000043 Rmu_sc0001563.1_g000044 Rmu_sc0001570.1_g000001 Rmu_sc0001629.1_g000016 Rmu_sc0001763.1_g000006 Rmu_sc0002011.1_g000013 Rmu_sc0002012.1_g000012 Rmu_sc0002268.1_g000026 Rmu_sc0002268.1_g000027 Rmu_sc0002321.1_g000021 Rmu_sc0002722.1_g000002 Rmu_sc0003032.1_g000006 Rmu_sc0003185.1_g000008 Rmu_sc0003238.1_g000006 Rmu_sc0003275.1_g000037 Rmu_sc0003275.1_g000038 Rmu_sc0003441.1_g000042 Rmu_sc0003748.1_g000027 Rmu_sc0004229.1_g000011 Rmu_sc0004283.1_g000041 Rmu_sc0004283.1_g000042 Rmu_sc0004283.1_g000043 Rmu_sc0004841.1_g000026 Rmu_sc0004841.1_g000031 Rmu_sc0004877.1_g000001 Rmu_sc0005039.1_g000012 Rmu_sc0005584.1_g000019 Rmu_sc0005584.1_g000020 Rmu_sc0005584.1_g000021 Rmu_sc0005600.1_g000021 Rmu_sc0005914.1_g000004 Rmu_sc0006139.1_g000006 Rmu_sc0006638.1_g000001 Rmu_sc0007228.1_g000001 Rmu_sc0007300.1_g000008 Rmu_sc0007486.1_g000002 Rmu_sc0007737.1_g000007 Rmu_sc0008328.1_g000015 Rmu_sc0009064.1_g000002 Rmu_sc0009315.1_g000006 Rmu_sc0009472.1_g000009 Rmu_sc0009774.1_g000016 Rmu_sc0009858.1_g000010 Rmu_sc0009858.1_g000011 Rmu_sc0010489.1_g000001 Rmu_sc0010579.1_g000002 Rmu_sc0011511.1_g000002 Rmu_sc0013359.1_g000001 Rmu_sc0014367.1_g000005 Rmu_sc0015624.1_g000003 Rmu_sc0016756.1_g000001 Rmu_sc0021286.1_g000001 Rmu_sc0021693.1_g000001 Rmu_sc0022538.1_g000001 Rmu_sc0030983.1_g000001 Rmu_sc0037682.1_g000001 Rmu_sc0041059.1_g000001 Rmu_ssc0000003.1_g000013 Rmu_ssc0000003.1_g000015 Rmu_ssc0000388.1_g000018 Rmu_ssc0000409.1_g000013 Rmu_ssc0000409.1_g000015 Rmu_ssc0000476.1_g000003
rosa_roxburghii Rroxscaffold_1G00017510 Rroxscaffold_1G00017520 Rroxscaffold_1G00017530 Rroxscaffold_1G00017990 Rroxscaffold_1G00031430 Rroxscaffold_1G00045790 Rroxscaffold_2G00108360 Rroxscaffold_2G00127910 Rroxscaffold_3G00218970 Rroxscaffold_3G00218980 Rroxscaffold_3G00218990 Rroxscaffold_3G00226450 Rroxscaffold_3G00226460 Rroxscaffold_3G00230570 Rroxscaffold_3G00230920 Rroxscaffold_4G00284360 Rroxscaffold_4G00292370 Rroxscaffold_4G00307090 Rroxscaffold_4G00319260 Rroxscaffold_4G00332580 Rroxscaffold_5G00341080 Rroxscaffold_5G00344620 Rroxscaffold_5G00358950 Rroxscaffold_5G00368600 Rroxscaffold_5G00368610 Rroxscaffold_5G00378350 Rroxscaffold_6G00388550 Rroxscaffold_6G00398560 Rroxscaffold_6G00398570 Rroxscaffold_7G00194900 Rroxscaffold_7G00194910 Rroxscaffold_7G00194920
rosa_rugosa Rorug05G0347200 Rorug06G0064400 Rorug06G0064500 Rorug07G0190700 Rorug07G0190800 Rorug07G0276200 Rorug07G0344800 Rorug07G0344900
rosa_samantha Rh1AG138000 Rh1AG138100 Rh1DG079100 Rh1DG264500 Rh1DG264600 Rh1DG275600 Rh1DG275700 Rh2AG474800 Rh2AG474900 Rh2AG557100 Rh2AG557200 Rh2AG567400 Rh2AG568000 Rh2AG568100 Rh3CG345000 Rh3CG351000 Rh3CG364300 Rh4AG186000 Rh5AG278000 Rh5DG280000 Rh5DG281700 Rh5DG447800 Rh7AG499400 Rh7BG470300 Rh7BG470400 Rh7DG197800 Rh7DG197900 Rh7DG198000 Rh7DG284800 Rh7DG311600 Rh7DG311700 Rh7DG329300 Rh7DG393300 Rh7DG393500 Rh7DG483800 Rh7DG483900
rosa_wichuraiana Rw0G003510 Rw1G021670 Rw2G016920 Rw3G020700 Rw7G028090 Rw7G031160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 348
AccB7I CCANNNNNTGG 1 cut(s) 1373
AccI GTMKAC 2 cut(s) 526, 741
AccII CGCG 4 cut(s) 89, 1104, 1424, 2003
AclWI GGATC 3 cut(s) 1356, 1456, 1680
AcsI RAATTY 4 cut(s) 128, 587, 1646, 2186
AcvI CACGTG 1 cut(s) 1067
AdeI CACNNNGTG 1 cut(s) 1562
AfaI GTAC 3 cut(s) 640, 1506, 1720
AfiI CCNNNNNNNGG 4 cut(s) 388, 1373, 1423, 1548
AflIII ACRYGT 1 cut(s) 1064
AgsI TTSAA 8 cut(s) 235, 616, 907, 1125, 1295, 1339, 1898, 2137
AhlI ACTAGT 1 cut(s) 341
AjiI CACGTC 1 cut(s) 1492
AjnI CCWGG 1 cut(s) 939
AleI CACNNNNGTG 2 cut(s) 1356, 1789
Alw21I GWGCWC 1 cut(s) 2175
Alw26I GTCTC 1 cut(s) 74
Alw44I GTGCAC 1 cut(s) 2171
AlwI GGATC 3 cut(s) 1356, 1456, 1680
Ama87I CYCGRG 1 cut(s) 6
AoxI GGCC 1 cut(s) 242
ApaLI GTGCAC 1 cut(s) 2171
ApeKI GCWGC 8 cut(s) 519, 598, 601, 679, 1526, 1934, 2156, 2222
ApoI RAATTY 4 cut(s) 128, 587, 1646, 2186
AspLEI GCGC 5 cut(s) 349, 956, 1151, 1177, 1424
AspS9I GGNCC 1 cut(s) 925
AsuC2I CCSGG 1 cut(s) 1443
AsuHPI GGTGA 7 cut(s) 297, 380, 842, 890, 1068, 1439, 1776
AvaI CYCGRG 1 cut(s) 6
AvaII GGWCC 1 cut(s) 925
AxyI CCTNAGG 2 cut(s) 912, 1088
BaeGI GKGCMC 1 cut(s) 2175
BaeI ACNNNNGTAYC 2 cut(s) 965, 998
BanII GRGCYC 2 cut(s) 1488, 2207
BbrPI CACGTG 1 cut(s) 1067
BbsI GAAGAC 2 cut(s) 12, 1971
Bbv12I GWGCWC 1 cut(s) 2175
BbvI GCAGC 8 cut(s) 531, 585, 613, 666, 1513, 1921, 2168, 2234
BccI CCATC 9 cut(s) 59, 162, 382, 454, 500, 989, 1265, 1292, 1403
BceAI ACGGC 3 cut(s) 229, 277, 2062
BciT130I CCWGG 1 cut(s) 941
BcnI CCSGG 1 cut(s) 1443
BcoDI GTCTC 1 cut(s) 74
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 7 cut(s) 215, 335, 342, 642, 714, 1026, 2097
BfmI CTRYAG 2 cut(s) 599, 1354
BfoI RGCGCY 2 cut(s) 957, 1152
BisI GCNGC 9 cut(s) 520, 599, 602, 680, 1527, 1935, 2157, 2220, 2223
BlsI GCNGC 9 cut(s) 521, 600, 603, 681, 1528, 1936, 2158, 2221, 2224
BmcAI AGTACT 1 cut(s) 640
Bme1390I CCNGG 2 cut(s) 941, 1443
Bme18I GGWCC 1 cut(s) 925
BmeT110I CYCGRG 1 cut(s) 6
BmgBI CACGTC 1 cut(s) 1492
BmgT120I GGNCC 1 cut(s) 925
BmiI GGNNCC 2 cut(s) 2011, 2204
BmrFI CCNGG 2 cut(s) 941, 1443
BmrI ACTGGG 1 cut(s) 2069
BmsI GCATC 7 cut(s) 438, 460, 1060, 1162, 1623, 1912, 2244
BmuI ACTGGG 1 cut(s) 2069
BpiI GAAGAC 2 cut(s) 12, 1971
BplI GAGNNNNNCTC 4 cut(s) 284, 316, 728, 760
BpmI CTGGAG 1 cut(s) 80
BpuEI CTTGAG 3 cut(s) 1699, 1889, 1967
BpuMI CCSGG 1 cut(s) 1443
Bsa29I ATCGAT 2 cut(s) 432, 2213
BsaAI YACGTR 1 cut(s) 1067
BsaBI GATNNNNATC 1 cut(s) 2234
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 5 cut(s) 87, 735, 939, 1508, 1787
BsaXI ACNNNNNCTCC 2 cut(s) 2063, 2093
Bsc4I CCNNNNNNNGG 4 cut(s) 388, 1373, 1423, 1548
Bse1I ACTGG 3 cut(s) 63, 637, 2064
Bse21I CCTNAGG 2 cut(s) 912, 1088
Bse3DI GCAATG 1 cut(s) 1947
Bse8I GATNNNNATC 1 cut(s) 2234
BseBI CCWGG 1 cut(s) 941
BseCI ATCGAT 2 cut(s) 432, 2213
BseDI CCNNGG 5 cut(s) 87, 735, 939, 1508, 1787
BseGI GGATG 3 cut(s) 70, 118, 445
BseJI GATNNNNATC 1 cut(s) 2234
BseLI CCNNNNNNNGG 4 cut(s) 388, 1373, 1423, 1548
BseMI GCAATG 1 cut(s) 1947
BseMII CTCAG 6 cut(s) 38, 126, 1102, 1608, 1659, 2258
BseNI ACTGG 3 cut(s) 63, 637, 2064
BseRI GAGGAG 2 cut(s) 1244, 2215
BseSI GKGCMC 1 cut(s) 2175
BseXI GCAGC 8 cut(s) 531, 585, 613, 666, 1513, 1921, 2168, 2234
Bsh1236I CGCG 4 cut(s) 89, 1104, 1424, 2003
BshFI GGCC 1 cut(s) 244
BshVI ATCGAT 2 cut(s) 432, 2213
BsiHKAI GWGCWC 1 cut(s) 2175
BsiHKCI CYCGRG 1 cut(s) 6
BsiSI CCGG 1 cut(s) 1442
BslFI GGGAC 2 cut(s) 1478, 2114
BslI CCNNNNNNNGG 4 cut(s) 388, 1373, 1423, 1548
BsmAI GTCTC 1 cut(s) 74
BsmFI GGGAC 2 cut(s) 1478, 2114
BsmI GAATGC 2 cut(s) 578, 930
BsnI GGCC 1 cut(s) 244
Bso31I GGTCTC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 6
Bsp1286I GDGCHC 3 cut(s) 1488, 2175, 2207
Bsp143I GATC 6 cut(s) 433, 1162, 1348, 1448, 1672, 1815
Bsp19I CCATGG 1 cut(s) 1787
Bsp68I TCGCGA 1 cut(s) 2003
BspANI GGCC 1 cut(s) 244
BspCNI CTCAG 6 cut(s) 37, 127, 1101, 1607, 1660, 2257
BspDI ATCGAT 2 cut(s) 432, 2213
BspFNI CGCG 4 cut(s) 89, 1104, 1424, 2003
BspLI GGNNCC 2 cut(s) 2011, 2204
BspMAI CTGCAG 2 cut(s) 603, 1358
BspPI GGATC 3 cut(s) 1356, 1456, 1680
BspTNI GGTCTC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 1947
BsrI ACTGG 3 cut(s) 63, 637, 2064
BssECI CCNNGG 5 cut(s) 87, 735, 939, 1508, 1787
BssMI GATC 6 cut(s) 433, 1162, 1348, 1448, 1672, 1815
BssNAI GTATAC 2 cut(s) 527, 742
BssT1I CCWWGG 2 cut(s) 1508, 1787
Bst1107I GTATAC 2 cut(s) 527, 742
Bst2UI CCWGG 1 cut(s) 941
Bst4CI ACNGT 7 cut(s) 256, 464, 697, 981, 1084, 1465, 1504
Bst6I CTCTTC 2 cut(s) 873, 1004
BstBAI YACGTR 1 cut(s) 1067
BstC8I GCNNGC 2 cut(s) 1705, 1939
BstDSI CCRYGG 2 cut(s) 87, 1787
BstEII GGTNACC 2 cut(s) 1274, 1427
BstF5I GGATG 3 cut(s) 70, 118, 445
BstFNI CGCG 4 cut(s) 89, 1104, 1424, 2003
BstH2I RGCGCY 2 cut(s) 957, 1152
BstHHI GCGC 5 cut(s) 349, 956, 1151, 1177, 1424
BstKTI GATC 6 cut(s) 436, 1165, 1351, 1451, 1675, 1818
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 6 cut(s) 433, 1162, 1348, 1448, 1672, 1815
BstMWI GCNNNNNNNGC 8 cut(s) 34, 344, 1197, 1931, 2009, 2153, 2165, 2179
BstNI CCWGG 1 cut(s) 941
BstPI GGTNACC 2 cut(s) 1274, 1427
BstSCI CCNGG 2 cut(s) 939, 1441
BstSFI CTRYAG 2 cut(s) 599, 1354
BstSLI GKGCMC 1 cut(s) 2175
BstUI CGCG 4 cut(s) 89, 1104, 1424, 2003
BstV1I GCAGC 8 cut(s) 531, 585, 613, 666, 1513, 1921, 2168, 2234
BstV2I GAAGAC 2 cut(s) 12, 1971
BstZ17I GTATAC 2 cut(s) 527, 742
Bsu15I ATCGAT 2 cut(s) 432, 2213
Bsu36I CCTNAGG 2 cut(s) 912, 1088
BsuRI GGCC 1 cut(s) 244
BsuTUI ATCGAT 2 cut(s) 432, 2213
BtgI CCRYGG 2 cut(s) 87, 1787
BtgZI GCGATG 2 cut(s) 401, 432
BtrI CACGTC 1 cut(s) 1492
BtsCI GGATG 3 cut(s) 70, 118, 445
BtsI GCAGTG 3 cut(s) 1351, 1363, 2232
BtsIMutI CAGTG 8 cut(s) 56, 693, 1080, 1351, 1363, 1377, 1665, 2232
BtuMI TCGCGA 1 cut(s) 2003
Cac8I GCNNGC 2 cut(s) 1705, 1939
CfoI GCGC 5 cut(s) 349, 956, 1151, 1177, 1424
Cfr13I GGNCC 1 cut(s) 925
Cfr42I CCGCGG 1 cut(s) 90
ClaI ATCGAT 2 cut(s) 432, 2213
Csp6I GTAC 3 cut(s) 639, 1505, 1719
CviAII CATG 4 cut(s) 700, 1114, 1788, 1874
CviQI GTAC 3 cut(s) 639, 1505, 1719
DpnI GATC 6 cut(s) 435, 1164, 1350, 1450, 1674, 1817
DpnII GATC 6 cut(s) 433, 1162, 1348, 1448, 1672, 1815
DraIII CACNNNGTG 1 cut(s) 1562
Eam1104I CTCTTC 2 cut(s) 873, 1004
EarI CTCTTC 2 cut(s) 873, 1004
EciI GGCGGA 1 cut(s) 815
Eco130I CCWWGG 2 cut(s) 1508, 1787
Eco24I GRGCYC 2 cut(s) 1488, 2207
Eco31I GGTCTC 1 cut(s) 74
Eco32I GATATC 1 cut(s) 2283
Eco47I GGWCC 1 cut(s) 925
Eco72I CACGTG 1 cut(s) 1067
Eco81I CCTNAGG 2 cut(s) 912, 1088
Eco88I CYCGRG 1 cut(s) 6
Eco91I GGTNACC 2 cut(s) 1274, 1427
EcoO65I GGTNACC 2 cut(s) 1274, 1427
EcoRII CCWGG 1 cut(s) 939
EcoRV GATATC 1 cut(s) 2283
EcoT14I CCWWGG 2 cut(s) 1508, 1787
EcoT22I ATGCAT 1 cut(s) 1927
EcoT38I GRGCYC 2 cut(s) 1488, 2207
ErhI CCWWGG 2 cut(s) 1508, 1787
FaeI CATG 4 cut(s) 703, 1117, 1791, 1877
FalI AAGNNNNNCTT 2 cut(s) 1976, 2008
FaqI GGGAC 2 cut(s) 1478, 2114
FatI CATG 4 cut(s) 699, 1113, 1787, 1873
FauI CCCGC 3 cut(s) 82, 1109, 1417
FauNDI CATATG 3 cut(s) 13, 364, 1225
FblI GTMKAC 2 cut(s) 526, 741
Fnu4HI GCNGC 9 cut(s) 520, 599, 602, 680, 1527, 1935, 2157, 2220, 2223
FokI GGATG 3 cut(s) 77, 125, 452
FriOI GRGCYC 2 cut(s) 1488, 2207
Fsp4HI GCNGC 9 cut(s) 520, 599, 602, 680, 1527, 1935, 2157, 2220, 2223
FspBI CTAG 7 cut(s) 215, 335, 342, 642, 714, 1026, 2097
FspI TGCGCA 1 cut(s) 348
GlaI GCGC 5 cut(s) 348, 955, 1150, 1176, 1423
GluI GCNGC 9 cut(s) 520, 599, 602, 680, 1527, 1935, 2157, 2220, 2223
GsuI CTGGAG 1 cut(s) 80
HaeII RGCGCY 2 cut(s) 957, 1152
HaeIII GGCC 1 cut(s) 244
HapII CCGG 1 cut(s) 1442
HhaI GCGC 5 cut(s) 349, 956, 1151, 1177, 1424
Hin1II CATG 4 cut(s) 703, 1117, 1791, 1877
Hin6I GCGC 5 cut(s) 347, 954, 1149, 1175, 1422
HinP1I GCGC 5 cut(s) 347, 954, 1149, 1175, 1422
HincII GTYRAC 2 cut(s) 1219, 1461
HindII GTYRAC 2 cut(s) 1219, 1461
HinfI GANTC 8 cut(s) 17, 317, 791, 1238, 1286, 1397, 1552, 2115
HpaII CCGG 1 cut(s) 1442
HphI GGTGA 7 cut(s) 297, 380, 842, 890, 1068, 1439, 1776
Hpy99I CGWCG 2 cut(s) 985, 2003
HpyAV CCTTC 9 cut(s) 95, 171, 184, 544, 734, 820, 886, 1146, 1464
HpyCH4III ACNGT 7 cut(s) 256, 464, 697, 981, 1084, 1465, 1504
HpyCH4IV ACGT 5 cut(s) 1066, 1129, 1329, 1491, 2291
HpyF10VI GCNNNNNNNGC 8 cut(s) 34, 344, 1197, 1931, 2009, 2153, 2165, 2179
HpySE526I ACGT 5 cut(s) 1066, 1129, 1329, 1491, 2291
Hsp92II CATG 4 cut(s) 703, 1117, 1791, 1877
HspAI GCGC 5 cut(s) 347, 954, 1149, 1175, 1422
KspI CCGCGG 1 cut(s) 90
Kzo9I GATC 6 cut(s) 433, 1162, 1348, 1448, 1672, 1815
LmnI GCTCC 5 cut(s) 595, 687, 1591, 2009, 2202
Lsp1109I GCAGC 8 cut(s) 531, 585, 613, 666, 1513, 1921, 2168, 2234
LweI GCATC 7 cut(s) 438, 460, 1060, 1162, 1623, 1912, 2244
MaeI CTAG 7 cut(s) 215, 335, 342, 642, 714, 1026, 2097
MaeII ACGT 5 cut(s) 1066, 1129, 1329, 1491, 2291
MalI GATC 6 cut(s) 435, 1164, 1350, 1450, 1674, 1817
MboI GATC 6 cut(s) 433, 1162, 1348, 1448, 1672, 1815
MhlI GDGCHC 3 cut(s) 1488, 2175, 2207
MluCI AATT 8 cut(s) 128, 230, 235, 422, 478, 587, 1646, 2186
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 2 cut(s) 963, 1447
Mph1103I ATGCAT 1 cut(s) 1927
MseI TTAA 6 cut(s) 201, 414, 627, 777, 1883, 2066
MslI CAYNNNNRTG 5 cut(s) 698, 704, 1356, 1786, 1789
MspA1I CMGCKG 4 cut(s) 89, 522, 604, 1282
MspI CCGG 1 cut(s) 1442
MspR9I CCNGG 2 cut(s) 941, 1443
Mva1269I GAATGC 2 cut(s) 578, 930
MvaI CCWGG 1 cut(s) 941
MvnI CGCG 4 cut(s) 89, 1104, 1424, 2003
MwoI GCNNNNNNNGC 8 cut(s) 34, 344, 1197, 1931, 2009, 2153, 2165, 2179
NciI CCSGG 1 cut(s) 1443
NcoI CCATGG 1 cut(s) 1787
NdeI CATATG 3 cut(s) 13, 364, 1225
NdeII GATC 6 cut(s) 433, 1162, 1348, 1448, 1672, 1815
NlaIII CATG 4 cut(s) 703, 1117, 1791, 1877
NlaIV GGNNCC 2 cut(s) 2011, 2204
NmuCI GTSAC 5 cut(s) 41, 830, 1181, 1330, 1427
NruI TCGCGA 1 cut(s) 2003
NsbI TGCGCA 1 cut(s) 348
NsiI ATGCAT 1 cut(s) 1927
OliI CACNNNNGTG 2 cut(s) 1356, 1789
PaeR7I CTCGAG 1 cut(s) 6
PctI GAATGC 2 cut(s) 578, 930
PfeI GAWTC 7 cut(s) 317, 791, 1238, 1286, 1397, 1552, 2115
PflMI CCANNNNNTGG 1 cut(s) 1373
PkrI GCNGC 9 cut(s) 521, 600, 603, 681, 1528, 1936, 2158, 2221, 2224
PleI GAGTC 1 cut(s) 25
PmaCI CACGTG 1 cut(s) 1067
PmlI CACGTG 1 cut(s) 1067
PpsI GAGTC 1 cut(s) 25
Ppu21I YACGTR 1 cut(s) 1067
Psp6I CCWGG 1 cut(s) 939
PspCI CACGTG 1 cut(s) 1067
PspEI GGTNACC 2 cut(s) 1274, 1427
PspGI CCWGG 1 cut(s) 939
PspN4I GGNNCC 2 cut(s) 2011, 2204
PspPI GGNCC 1 cut(s) 925
PspXI VCTCGAGB 1 cut(s) 6
PstI CTGCAG 2 cut(s) 603, 1358
PvuII CAGCTG 2 cut(s) 604, 1282
RruI TCGCGA 1 cut(s) 2003
RsaI GTAC 3 cut(s) 640, 1506, 1720
RsaNI GTAC 3 cut(s) 639, 1505, 1719
RseI CAYNNNNRTG 5 cut(s) 698, 704, 1356, 1786, 1789
SacII CCGCGG 1 cut(s) 90
SaqAI TTAA 6 cut(s) 201, 414, 627, 777, 1883, 2066
SatI GCNGC 9 cut(s) 520, 599, 602, 680, 1527, 1935, 2157, 2220, 2223
Sau3AI GATC 6 cut(s) 433, 1162, 1348, 1448, 1672, 1815
Sau96I GGNCC 1 cut(s) 925
ScaI AGTACT 1 cut(s) 640
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 2 cut(s) 941, 1443
SduI GDGCHC 3 cut(s) 1488, 2175, 2207
SfaNI GCATC 7 cut(s) 438, 460, 1060, 1162, 1623, 1912, 2244
SfcI CTRYAG 2 cut(s) 599, 1354
Sfr274I CTCGAG 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 90
SgrBI CCGCGG 1 cut(s) 90
SinI GGWCC 1 cut(s) 925
SlaI CTCGAG 1 cut(s) 6
SmiMI CAYNNNNRTG 5 cut(s) 698, 704, 1356, 1786, 1789
SmlI CTYRAG 4 cut(s) 6, 1678, 1904, 1982
SmoI CTYRAG 4 cut(s) 6, 1678, 1904, 1982
SpeI ACTAGT 1 cut(s) 341
Sse9I AATT 8 cut(s) 128, 230, 235, 422, 478, 587, 1646, 2186
SspI AATATT 1 cut(s) 1766
SspMI CTAG 7 cut(s) 215, 335, 342, 642, 714, 1026, 2097
StyD4I CCNGG 2 cut(s) 939, 1441
StyI CCWWGG 2 cut(s) 1508, 1787
TaaI ACNGT 7 cut(s) 256, 464, 697, 981, 1084, 1465, 1504
TaiI ACGT 5 cut(s) 1069, 1132, 1332, 1494, 2294
TaqI TCGA 6 cut(s) 7, 432, 746, 1324, 1818, 2213
TasI AATT 8 cut(s) 128, 230, 235, 422, 478, 587, 1646, 2186
TatI WGTACW 2 cut(s) 638, 1718
TauI GCSGC 1 cut(s) 2222
TfiI GAWTC 7 cut(s) 317, 791, 1238, 1286, 1397, 1552, 2115
Tru1I TTAA 6 cut(s) 201, 414, 627, 777, 1883, 2066
Tru9I TTAA 6 cut(s) 201, 414, 627, 777, 1883, 2066
TscAI CASTG 8 cut(s) 63, 700, 1087, 1358, 1363, 1384, 1672, 2232
TseFI GTSAC 5 cut(s) 41, 830, 1181, 1330, 1427
TseI GCWGC 8 cut(s) 519, 598, 601, 679, 1526, 1934, 2156, 2222
Tsp45I GTSAC 5 cut(s) 41, 830, 1181, 1330, 1427
TspDTI ATGAA 2 cut(s) 1737, 1862
TspGWI ACGGA 2 cut(s) 310, 1987
TspRI CASTG 8 cut(s) 63, 700, 1087, 1358, 1363, 1384, 1672, 2232
Van91I CCANNNNNTGG 1 cut(s) 1373
VneI GTGCAC 1 cut(s) 2171
VpaK11BI GGWCC 1 cut(s) 925
XapI RAATTY 4 cut(s) 128, 587, 1646, 2186
XbaI TCTAGA 1 cut(s) 713
XhoI CTCGAG 1 cut(s) 6
XmiI GTMKAC 2 cut(s) 526, 741
XspI CTAG 7 cut(s) 215, 335, 342, 642, 714, 1026, 2097
ZrmI AGTACT 1 cut(s) 640
Zsp2I ATGCAT 1 cut(s) 1927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.