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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 255 |
| AclI | AACGTT | 1 cut(s) | 92 |
| AflIII | ACRYGT | 1 cut(s) | 274 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 264, 296 |
| Alw26I | GTCTC | 2 cut(s) | 59, 184 |
| Ama87I | CYCGRG | 1 cut(s) | 56 |
| AvaI | CYCGRG | 1 cut(s) | 56 |
| BccI | CCATC | 1 cut(s) | 14 |
| BcoDI | GTCTC | 2 cut(s) | 59, 184 |
| BfaI | CTAG | 1 cut(s) | 156 |
| BmeT110I | CYCGRG | 1 cut(s) | 56 |
| BmiI | GGNNCC | 1 cut(s) | 79 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 242, 274 |
| BpuEI | CTTGAG | 1 cut(s) | 118 |
| BsaJI | CCNNGG | 1 cut(s) | 213 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 101, 131 |
| Bse1I | ACTGG | 1 cut(s) | 151 |
| BseDI | CCNNGG | 1 cut(s) | 213 |
| BseGI | GGATG | 1 cut(s) | 307 |
| BseMII | CTCAG | 2 cut(s) | 60, 240 |
| BseNI | ACTGG | 1 cut(s) | 151 |
| BsiHKCI | CYCGRG | 1 cut(s) | 56 |
| BslFI | GGGAC | 1 cut(s) | 166 |
| BsmAI | GTCTC | 2 cut(s) | 59, 184 |
| BsmFI | GGGAC | 1 cut(s) | 166 |
| BsoBI | CYCGRG | 1 cut(s) | 56 |
| Bsp19I | CCATGG | 1 cut(s) | 213 |
| BspCNI | CTCAG | 2 cut(s) | 59, 241 |
| BspLI | GGNNCC | 1 cut(s) | 79 |
| BsrI | ACTGG | 1 cut(s) | 151 |
| BssECI | CCNNGG | 1 cut(s) | 213 |
| BssNAI | GTATAC | 1 cut(s) | 256 |
| BssT1I | CCWWGG | 1 cut(s) | 213 |
| Bst1107I | GTATAC | 1 cut(s) | 256 |
| Bst4CI | ACNGT | 1 cut(s) | 71 |
| BstDEI | CTNAG | 2 cut(s) | 46, 249 |
| BstDSI | CCRYGG | 1 cut(s) | 213 |
| BstF5I | GGATG | 1 cut(s) | 307 |
| BstMAI | GTCTC | 2 cut(s) | 59, 184 |
| BstZ17I | GTATAC | 1 cut(s) | 256 |
| BtgI | CCRYGG | 1 cut(s) | 213 |
| BtsCI | GGATG | 1 cut(s) | 307 |
| BtsI | GCAGTG | 2 cut(s) | 122, 207 |
| BtsIMutI | CAGTG | 2 cut(s) | 122, 207 |
| CviAII | CATG | 2 cut(s) | 214, 223 |
| CviJI | RGCY | 2 cut(s) | 78, 173 |
| CviKI_1 | RGCY | 2 cut(s) | 78, 173 |
| DdeI | CTNAG | 2 cut(s) | 46, 249 |
| Eco130I | CCWWGG | 1 cut(s) | 213 |
| Eco88I | CYCGRG | 1 cut(s) | 56 |
| EcoT14I | CCWWGG | 1 cut(s) | 213 |
| ErhI | CCWWGG | 1 cut(s) | 213 |
| FaeI | CATG | 2 cut(s) | 217, 226 |
| FaiI | YATR | 8 cut(s) | 41, 215, 224, 244, 246, 256, 269, 331 |
| FaqI | GGGAC | 1 cut(s) | 166 |
| FatI | CATG | 2 cut(s) | 213, 222 |
| FblI | GTMKAC | 1 cut(s) | 255 |
| FokI | GGATG | 1 cut(s) | 314 |
| FspBI | CTAG | 1 cut(s) | 156 |
| Hin1II | CATG | 2 cut(s) | 217, 226 |
| Hpy166II | GTNNAC | 2 cut(s) | 256, 316 |
| Hpy188I | TCNGA | 4 cut(s) | 15, 49, 144, 168 |
| Hpy188III | TCNNGA | 3 cut(s) | 58, 97, 130 |
| Hpy8I | GTNNAC | 2 cut(s) | 256, 316 |
| HpyAV | CCTTC | 2 cut(s) | 58, 248 |
| HpyCH4III | ACNGT | 1 cut(s) | 71 |
| HpyCH4IV | ACGT | 2 cut(s) | 92, 274 |
| HpyCH4V | TGCA | 1 cut(s) | 115 |
| HpyF3I | CTNAG | 2 cut(s) | 46, 249 |
| HpySE526I | ACGT | 2 cut(s) | 92, 274 |
| Hsp92II | CATG | 2 cut(s) | 217, 226 |
| LmnI | GCTCC | 1 cut(s) | 83 |
| LpnPI | CCDG | 2 cut(s) | 115, 132 |
| MaeI | CTAG | 1 cut(s) | 156 |
| MaeII | ACGT | 2 cut(s) | 92, 274 |
| MboII | GAAGA | 1 cut(s) | 73 |
| MnlI | CCTC | 4 cut(s) | 93, 126, 244, 304 |
| MseI | TTAA | 2 cut(s) | 234, 291 |
| MslI | CAYNNNNRTG | 1 cut(s) | 212 |
| NcoI | CCATGG | 1 cut(s) | 213 |
| NlaIII | CATG | 2 cut(s) | 217, 226 |
| NlaIV | GGNNCC | 1 cut(s) | 79 |
| Psp1406I | AACGTT | 1 cut(s) | 92 |
| PspN4I | GGNNCC | 1 cut(s) | 79 |
| RseI | CAYNNNNRTG | 1 cut(s) | 212 |
| SaqAI | TTAA | 2 cut(s) | 234, 291 |
| SetI | ASST | 9 cut(s) | 95, 104, 137, 161, 240, 255, 277, 298, 315 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 212 |
| SmlI | CTYRAG | 1 cut(s) | 97 |
| SmoI | CTYRAG | 1 cut(s) | 97 |
| SspMI | CTAG | 1 cut(s) | 156 |
| StyI | CCWWGG | 1 cut(s) | 213 |
| TaaI | ACNGT | 1 cut(s) | 71 |
| TaiI | ACGT | 2 cut(s) | 95, 277 |
| TaqI | TCGA | 1 cut(s) | 260 |
| Tru1I | TTAA | 2 cut(s) | 234, 291 |
| Tru9I | TTAA | 2 cut(s) | 234, 291 |
| TscAI | CASTG | 2 cut(s) | 122, 214 |
| TspRI | CASTG | 2 cut(s) | 122, 214 |
| XmiI | GTMKAC | 1 cut(s) | 255 |
| XspI | CTAG | 1 cut(s) | 156 |