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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 291 |
| AciI | CCGC | 4 cut(s) | 26, 98, 235, 349 |
| AcsI | RAATTY | 1 cut(s) | 138 |
| AfaI | GTAC | 2 cut(s) | 16, 190 |
| AgeI | ACCGGT | 1 cut(s) | 182 |
| AgsI | TTSAA | 1 cut(s) | 78 |
| AluBI | AGCT | 2 cut(s) | 64, 149 |
| AluI | AGCT | 2 cut(s) | 64, 149 |
| ApeKI | GCWGC | 2 cut(s) | 149, 229 |
| ApoI | RAATTY | 1 cut(s) | 138 |
| AsiGI | ACCGGT | 1 cut(s) | 182 |
| AsuNHI | GCTAGC | 1 cut(s) | 4 |
| BbvI | GCAGC | 2 cut(s) | 136, 216 |
| BfaI | CTAG | 3 cut(s) | 5, 192, 264 |
| BisI | GCNGC | 2 cut(s) | 150, 230 |
| BlsI | GCNGC | 2 cut(s) | 151, 231 |
| BmcAI | AGTACT | 1 cut(s) | 190 |
| BmtI | GCTAGC | 1 cut(s) | 8 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 278, 310 |
| BsaJI | CCNNGG | 1 cut(s) | 285 |
| BsaWI | WCCGGW | 1 cut(s) | 182 |
| Bse118I | RCCGGY | 1 cut(s) | 182 |
| Bse1I | ACTGG | 1 cut(s) | 16 |
| BseDI | CCNNGG | 1 cut(s) | 285 |
| BseGI | GGATG | 1 cut(s) | 88 |
| BseNI | ACTGG | 1 cut(s) | 16 |
| BseRI | GAGGAG | 1 cut(s) | 24 |
| BseXI | GCAGC | 2 cut(s) | 136, 216 |
| BshTI | ACCGGT | 1 cut(s) | 182 |
| BsiSI | CCGG | 1 cut(s) | 183 |
| BsmI | GAATGC | 1 cut(s) | 129 |
| Bsp143I | GATC | 1 cut(s) | 67 |
| BspACI | CCGC | 4 cut(s) | 26, 98, 235, 349 |
| BspOI | GCTAGC | 1 cut(s) | 8 |
| BsrFI | RCCGGY | 1 cut(s) | 182 |
| BsrI | ACTGG | 1 cut(s) | 16 |
| BssAI | RCCGGY | 1 cut(s) | 182 |
| BssECI | CCNNGG | 1 cut(s) | 285 |
| BssMI | GATC | 1 cut(s) | 67 |
| BssNAI | GTATAC | 1 cut(s) | 292 |
| Bst1107I | GTATAC | 1 cut(s) | 292 |
| Bst4CI | ACNGT | 1 cut(s) | 247 |
| BstC8I | GCNNGC | 2 cut(s) | 6, 30 |
| BstF5I | GGATG | 1 cut(s) | 88 |
| BstKTI | GATC | 1 cut(s) | 70 |
| BstMBI | GATC | 1 cut(s) | 67 |
| BstV1I | GCAGC | 2 cut(s) | 136, 216 |
| BstZ17I | GTATAC | 1 cut(s) | 292 |
| BtsCI | GGATG | 1 cut(s) | 88 |
| Cac8I | GCNNGC | 2 cut(s) | 6, 30 |
| Cfr10I | RCCGGY | 1 cut(s) | 182 |
| Csp6I | GTAC | 2 cut(s) | 15, 189 |
| CspAI | ACCGGT | 1 cut(s) | 182 |
| CviAII | CATG | 1 cut(s) | 250 |
| CviJI | RGCY | 3 cut(s) | 64, 149, 229 |
| CviKI_1 | RGCY | 3 cut(s) | 64, 149, 229 |
| CviQI | GTAC | 2 cut(s) | 15, 189 |
| DpnI | GATC | 1 cut(s) | 69 |
| DpnII | GATC | 1 cut(s) | 67 |
| FaeI | CATG | 1 cut(s) | 253 |
| FaiI | YATR | 6 cut(s) | 55, 107, 251, 282, 292, 305 |
| FatI | CATG | 1 cut(s) | 249 |
| FauI | CCCGC | 1 cut(s) | 91 |
| FblI | GTMKAC | 1 cut(s) | 291 |
| Fnu4HI | GCNGC | 2 cut(s) | 150, 230 |
| FokI | GGATG | 1 cut(s) | 95 |
| Fsp4HI | GCNGC | 2 cut(s) | 150, 230 |
| FspBI | CTAG | 3 cut(s) | 5, 192, 264 |
| GluI | GCNGC | 2 cut(s) | 150, 230 |
| HapII | CCGG | 1 cut(s) | 183 |
| Hin1II | CATG | 1 cut(s) | 253 |
| HindIII | AAGCTT | 1 cut(s) | 62 |
| HinfI | GANTC | 1 cut(s) | 341 |
| HpaII | CCGG | 1 cut(s) | 183 |
| Hpy166II | GTNNAC | 1 cut(s) | 292 |
| Hpy188I | TCNGA | 2 cut(s) | 72, 204 |
| Hpy188III | TCNNGA | 1 cut(s) | 264 |
| Hpy8I | GTNNAC | 1 cut(s) | 292 |
| HpyAV | CCTTC | 2 cut(s) | 86, 284 |
| HpyCH4III | ACNGT | 1 cut(s) | 247 |
| HpyCH4IV | ACGT | 1 cut(s) | 155 |
| HpyCH4V | TGCA | 1 cut(s) | 152 |
| HpySE526I | ACGT | 1 cut(s) | 155 |
| Hsp92II | CATG | 1 cut(s) | 253 |
| Kzo9I | GATC | 1 cut(s) | 67 |
| LmnI | GCTCC | 3 cut(s) | 37, 146, 237 |
| LpnPI | CCDG | 2 cut(s) | 43, 196 |
| Lsp1109I | GCAGC | 2 cut(s) | 136, 216 |
| MaeI | CTAG | 3 cut(s) | 5, 192, 264 |
| MaeII | ACGT | 1 cut(s) | 155 |
| MalI | GATC | 1 cut(s) | 69 |
| MboI | GATC | 1 cut(s) | 67 |
| MluCI | AATT | 1 cut(s) | 138 |
| MnlI | CCTC | 4 cut(s) | 45, 162, 219, 280 |
| MseI | TTAA | 3 cut(s) | 165, 177, 327 |
| MslI | CAYNNNNRTG | 2 cut(s) | 248, 254 |
| MspI | CCGG | 1 cut(s) | 183 |
| Mva1269I | GAATGC | 1 cut(s) | 129 |
| NdeII | GATC | 1 cut(s) | 67 |
| NheI | GCTAGC | 1 cut(s) | 4 |
| NlaIII | CATG | 1 cut(s) | 253 |
| PctI | GAATGC | 1 cut(s) | 129 |
| PfeI | GAWTC | 1 cut(s) | 341 |
| PinAI | ACCGGT | 1 cut(s) | 182 |
| PkrI | GCNGC | 2 cut(s) | 151, 231 |
| RsaI | GTAC | 2 cut(s) | 16, 190 |
| RsaNI | GTAC | 2 cut(s) | 15, 189 |
| RseI | CAYNNNNRTG | 2 cut(s) | 248, 254 |
| SaqAI | TTAA | 3 cut(s) | 165, 177, 327 |
| SatI | GCNGC | 2 cut(s) | 150, 230 |
| Sau3AI | GATC | 1 cut(s) | 67 |
| ScaI | AGTACT | 1 cut(s) | 190 |
| SetI | ASST | 9 cut(s) | 66, 97, 151, 158, 173, 197, 276, 291, 334 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 248, 254 |
| Sse9I | AATT | 1 cut(s) | 138 |
| SsiI | CCGC | 4 cut(s) | 26, 98, 235, 349 |
| SspMI | CTAG | 3 cut(s) | 5, 192, 264 |
| TaaI | ACNGT | 1 cut(s) | 247 |
| TaiI | ACGT | 1 cut(s) | 158 |
| TaqI | TCGA | 1 cut(s) | 296 |
| TaqII | GACCGA | 1 cut(s) | 283 |
| TasI | AATT | 1 cut(s) | 138 |
| TatI | WGTACW | 1 cut(s) | 188 |
| TfiI | GAWTC | 1 cut(s) | 341 |
| Tru1I | TTAA | 3 cut(s) | 165, 177, 327 |
| Tru9I | TTAA | 3 cut(s) | 165, 177, 327 |
| TseI | GCWGC | 2 cut(s) | 149, 229 |
| XapI | RAATTY | 1 cut(s) | 138 |
| XbaI | TCTAGA | 1 cut(s) | 263 |
| XmiI | GTMKAC | 1 cut(s) | 291 |
| XspI | CTAG | 3 cut(s) | 5, 192, 264 |
| ZrmI | AGTACT | 1 cut(s) | 190 |