Plant mobile domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2061 |
| AarI | CACCTGC | 1 cut(s) | 1652 |
| Acc16I | TGCGCA | 1 cut(s) | 2870 |
| Acc36I | ACCTGC | 2 cut(s) | 532, 1652 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 494 |
| AccI | GTMKAC | 2 cut(s) | 826, 1569 |
| AccII | CGCG | 1 cut(s) | 747 |
| AciI | CCGC | 5 cut(s) | 1028, 1309, 1427, 2501, 2699 |
| AclWI | GGATC | 1 cut(s) | 2788 |
| AcoI | YGGCCR | 1 cut(s) | 260 |
| AcsI | RAATTY | 5 cut(s) | 1231, 1739, 2484, 2802, 2890 |
| AcuI | CTGAAG | 3 cut(s) | 1584, 2475, 2796 |
| AfaI | GTAC | 8 cut(s) | 980, 1404, 1527, 1582, 1852, 2253, 2621, 2934 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 457, 494, 650, 783, 1503, 2348 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 960 |
| AhlI | ACTAGT | 1 cut(s) | 2357 |
| AjiI | CACGTC | 1 cut(s) | 2852 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 481, 513, 1689, 1721, 2576, 2608 |
| AluBI | AGCT | 6 cut(s) | 67, 283, 1210, 1450, 1619, 2337 |
| AluI | AGCT | 6 cut(s) | 67, 283, 1210, 1450, 1619, 2337 |
| Alw21I | GWGCWC | 3 cut(s) | 189, 1212, 2746 |
| Alw26I | GTCTC | 5 cut(s) | 384, 1148, 2309, 2451, 2559 |
| Alw44I | GTGCAC | 2 cut(s) | 185, 2742 |
| AlwI | GGATC | 1 cut(s) | 2788 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 320 |
| Ama87I | CYCGRG | 2 cut(s) | 1090, 1839 |
| AoxI | GGCC | 5 cut(s) | 260, 294, 558, 2323, 2771 |
| ApaLI | GTGCAC | 2 cut(s) | 185, 2742 |
| ApeKI | GCWGC | 4 cut(s) | 320, 999, 1190, 1467 |
| ApoI | RAATTY | 5 cut(s) | 1231, 1739, 2484, 2802, 2890 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1006, 1038 |
| AseI | ATTAAT | 1 cut(s) | 2133 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2384 |
| AspLEI | GCGC | 4 cut(s) | 747, 1713, 2443, 2871 |
| AspS9I | GGNCC | 8 cut(s) | 558, 797, 1264, 1429, 1487, 1988, 2045, 2504 |
| AsuC2I | CCSGG | 1 cut(s) | 645 |
| AsuHPI | GGTGA | 7 cut(s) | 613, 1546, 1637, 1676, 1825, 2156, 2228 |
| AvaI | CYCGRG | 2 cut(s) | 1090, 1839 |
| AvaII | GGWCC | 7 cut(s) | 797, 1264, 1429, 1487, 1988, 2045, 2504 |
| AxyI | CCTNAGG | 1 cut(s) | 201 |
| BaeGI | GKGCMC | 2 cut(s) | 189, 2746 |
| BalI | TGGCCA | 1 cut(s) | 262 |
| BanII | GRGCYC | 1 cut(s) | 1212 |
| BauI | CACGAG | 2 cut(s) | 298, 1983 |
| BbsI | GAAGAC | 1 cut(s) | 703 |
| Bbv12I | GWGCWC | 3 cut(s) | 189, 1212, 2746 |
| BbvI | GCAGC | 4 cut(s) | 307, 1011, 1177, 1479 |
| BccI | CCATC | 8 cut(s) | 37, 236, 1444, 1571, 2135, 2212, 2642, 2815 |
| BceAI | ACGGC | 4 cut(s) | 130, 570, 654, 2667 |
| BclI | TGATCA | 2 cut(s) | 2020, 2098 |
| BcnI | CCSGG | 1 cut(s) | 645 |
| BcoDI | GTCTC | 5 cut(s) | 384, 1148, 2309, 2451, 2559 |
| BcuI | ACTAGT | 1 cut(s) | 2357 |
| BfmI | CTRYAG | 2 cut(s) | 1194, 2813 |
| BfoI | RGCGCY | 1 cut(s) | 1714 |
| BfuAI | ACCTGC | 2 cut(s) | 532, 1652 |
| BisI | GCNGC | 6 cut(s) | 321, 1000, 1028, 1191, 1310, 1468 |
| BlsI | GCNGC | 6 cut(s) | 322, 1001, 1029, 1192, 1311, 1469 |
| Bme1390I | CCNGG | 1 cut(s) | 645 |
| Bme18I | GGWCC | 7 cut(s) | 797, 1264, 1429, 1487, 1988, 2045, 2504 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 960 |
| BmeT110I | CYCGRG | 2 cut(s) | 1090, 1839 |
| BmgBI | CACGTC | 1 cut(s) | 2852 |
| BmgT120I | GGNCC | 8 cut(s) | 558, 797, 1264, 1429, 1487, 1988, 2045, 2504 |
| BmiI | GGNNCC | 5 cut(s) | 559, 948, 1488, 2046, 2505 |
| BmrFI | CCNGG | 1 cut(s) | 645 |
| BmrI | ACTGGG | 1 cut(s) | 180 |
| BmsI | GCATC | 4 cut(s) | 1296, 2102, 2116, 2665 |
| BmuI | ACTGGG | 1 cut(s) | 180 |
| BpiI | GAAGAC | 1 cut(s) | 703 |
| BpmI | CTGGAG | 4 cut(s) | 547, 1121, 1977, 2271 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1620, 2333 |
| BpuEI | CTTGAG | 5 cut(s) | 257, 2024, 2304, 2862, 2876 |
| BpuMI | CCSGG | 1 cut(s) | 645 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2306 |
| BsaI | GGTCTC | 1 cut(s) | 1148 |
| BsaJI | CCNNGG | 3 cut(s) | 643, 969, 2121 |
| BsaWI | WCCGGW | 1 cut(s) | 2168 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 181, 211, 624, 654 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 457, 494, 650, 783, 1503, 2348 |
| Bse118I | RCCGGY | 1 cut(s) | 776 |
| Bse1I | ACTGG | 6 cut(s) | 175, 499, 564, 1994, 2791, 2940 |
| Bse21I | CCTNAGG | 1 cut(s) | 201 |
| Bse3DI | GCAATG | 4 cut(s) | 687, 1375, 2185, 2427 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2306 |
| BseDI | CCNNGG | 3 cut(s) | 643, 969, 2121 |
| BseGI | GGATG | 7 cut(s) | 247, 376, 581, 1108, 2131, 2200, 2959 |
| BseJI | GATNNNNATC | 1 cut(s) | 2306 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 457, 494, 650, 783, 1503, 2348 |
| BseMI | GCAATG | 4 cut(s) | 687, 1375, 2185, 2427 |
| BseMII | CTCAG | 6 cut(s) | 192, 543, 1137, 1454, 1487, 2514 |
| BseNI | ACTGG | 6 cut(s) | 175, 499, 564, 1994, 2791, 2940 |
| BseRI | GAGGAG | 2 cut(s) | 1971, 2426 |
| BseSI | GKGCMC | 2 cut(s) | 189, 2746 |
| BseXI | GCAGC | 4 cut(s) | 307, 1011, 1177, 1479 |
| Bsh1236I | CGCG | 1 cut(s) | 747 |
| BshFI | GGCC | 5 cut(s) | 262, 296, 560, 2325, 2773 |
| BsiHKAI | GWGCWC | 3 cut(s) | 189, 1212, 2746 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1090, 1839 |
| BsiSI | CCGG | 3 cut(s) | 645, 777, 2169 |
| BslFI | GGGAC | 4 cut(s) | 626, 1500, 1500, 1919 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 457, 494, 650, 783, 1503, 2348 |
| BsmAI | GTCTC | 5 cut(s) | 384, 1148, 2309, 2451, 2559 |
| BsmFI | GGGAC | 4 cut(s) | 626, 1500, 1500, 1919 |
| BsmI | GAATGC | 1 cut(s) | 1879 |
| BsnI | GGCC | 5 cut(s) | 262, 296, 560, 2325, 2773 |
| Bso31I | GGTCTC | 1 cut(s) | 1148 |
| BsoBI | CYCGRG | 2 cut(s) | 1090, 1839 |
| Bsp1286I | GDGCHC | 3 cut(s) | 189, 1212, 2746 |
| Bsp1407I | TGTACA | 2 cut(s) | 978, 2619 |
| Bsp143I | GATC | 8 cut(s) | 70, 220, 1270, 2020, 2098, 2149, 2560, 2780 |
| Bsp19I | CCATGG | 1 cut(s) | 2121 |
| BspACI | CCGC | 5 cut(s) | 1028, 1309, 1427, 2501, 2699 |
| BspANI | GGCC | 5 cut(s) | 262, 296, 560, 2325, 2773 |
| BspCNI | CTCAG | 6 cut(s) | 193, 542, 1138, 1453, 1486, 2515 |
| BspFNI | CGCG | 1 cut(s) | 747 |
| BspHI | TCATGA | 2 cut(s) | 2239, 2388 |
| BspLI | GGNNCC | 5 cut(s) | 559, 948, 1488, 2046, 2505 |
| BspMAI | CTGCAG | 1 cut(s) | 2817 |
| BspMI | ACCTGC | 2 cut(s) | 532, 1652 |
| BspPI | GGATC | 1 cut(s) | 2788 |
| BspQI | GCTCTTC | 2 cut(s) | 735, 1173 |
| BspTNI | GGTCTC | 1 cut(s) | 1148 |
| BsrDI | GCAATG | 4 cut(s) | 687, 1375, 2185, 2427 |
| BsrFI | RCCGGY | 1 cut(s) | 776 |
| BsrGI | TGTACA | 2 cut(s) | 978, 2619 |
| BsrI | ACTGG | 6 cut(s) | 175, 499, 564, 1994, 2791, 2940 |
| BssAI | RCCGGY | 1 cut(s) | 776 |
| BssECI | CCNNGG | 3 cut(s) | 643, 969, 2121 |
| BssMI | GATC | 8 cut(s) | 70, 220, 1270, 2020, 2098, 2149, 2560, 2780 |
| BssNAI | GTATAC | 1 cut(s) | 1570 |
| BssSI | CACGAG | 2 cut(s) | 298, 1983 |
| BssT1I | CCWWGG | 2 cut(s) | 969, 2121 |
| Bst1107I | GTATAC | 1 cut(s) | 1570 |
| Bst2BI | CACGAG | 2 cut(s) | 298, 1983 |
| Bst4CI | ACNGT | 4 cut(s) | 927, 1081, 1525, 2960 |
| Bst6I | CTCTTC | 6 cut(s) | 387, 735, 1173, 1901, 1944, 2856 |
| BstAUI | TGTACA | 2 cut(s) | 978, 2619 |
| BstDSI | CCRYGG | 1 cut(s) | 2121 |
| BstEII | GGTNACC | 1 cut(s) | 1319 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2346 |
| BstF5I | GGATG | 7 cut(s) | 247, 376, 581, 1108, 2131, 2200, 2959 |
| BstFNI | CGCG | 1 cut(s) | 747 |
| BstH2I | RGCGCY | 1 cut(s) | 1714 |
| BstHHI | GCGC | 4 cut(s) | 747, 1713, 2443, 2871 |
| BstKTI | GATC | 8 cut(s) | 73, 223, 1273, 2023, 2101, 2152, 2563, 2783 |
| BstMAI | GTCTC | 5 cut(s) | 384, 1148, 2309, 2451, 2559 |
| BstMBI | GATC | 8 cut(s) | 70, 220, 1270, 2020, 2098, 2149, 2560, 2780 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 773, 1717, 2113, 2438, 2471, 2519 |
| BstNSI | RCATGY | 1 cut(s) | 2285 |
| BstPI | GGTNACC | 1 cut(s) | 1319 |
| BstSCI | CCNGG | 1 cut(s) | 643 |
| BstSFI | CTRYAG | 2 cut(s) | 1194, 2813 |
| BstSLI | GKGCMC | 2 cut(s) | 189, 2746 |
| BstUI | CGCG | 1 cut(s) | 747 |
| BstV1I | GCAGC | 4 cut(s) | 307, 1011, 1177, 1479 |
| BstV2I | GAAGAC | 1 cut(s) | 703 |
| BstZ17I | GTATAC | 1 cut(s) | 1570 |
| Bsu36I | CCTNAGG | 1 cut(s) | 201 |
| BsuRI | GGCC | 5 cut(s) | 262, 296, 560, 2325, 2773 |
| BtgI | CCRYGG | 1 cut(s) | 2121 |
| BtgZI | GCGATG | 2 cut(s) | 132, 1590 |
| BtrI | CACGTC | 1 cut(s) | 2852 |
| BtsCI | GGATG | 7 cut(s) | 247, 376, 581, 1108, 2131, 2200, 2959 |
| BtsI | GCAGTG | 2 cut(s) | 1190, 2460 |
| BtsIMutI | CAGTG | 7 cut(s) | 571, 1086, 1190, 1255, 1278, 2460, 2798 |
| BveI | ACCTGC | 2 cut(s) | 532, 1652 |
| CaiI | CAGNNNCTG | 1 cut(s) | 320 |
| CciI | TCATGA | 2 cut(s) | 2239, 2388 |
| CfoI | GCGC | 4 cut(s) | 747, 1713, 2443, 2871 |
| Cfr10I | RCCGGY | 1 cut(s) | 776 |
| Cfr13I | GGNCC | 8 cut(s) | 558, 797, 1264, 1429, 1487, 1988, 2045, 2504 |
| Csp6I | GTAC | 8 cut(s) | 979, 1403, 1526, 1581, 1851, 2252, 2620, 2933 |
| CviQI | GTAC | 8 cut(s) | 979, 1403, 1526, 1581, 1851, 2252, 2620, 2933 |
| DpnI | GATC | 8 cut(s) | 72, 222, 1272, 2022, 2100, 2151, 2562, 2782 |
| DpnII | GATC | 8 cut(s) | 70, 220, 1270, 2020, 2098, 2149, 2560, 2780 |
| DraI | TTTAAA | 2 cut(s) | 181, 1605 |
| DriI | GACNNNNNGTC | 1 cut(s) | 960 |
| EaeI | YGGCCR | 1 cut(s) | 260 |
| Eam1104I | CTCTTC | 6 cut(s) | 387, 735, 1173, 1901, 1944, 2856 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 960 |
| EarI | CTCTTC | 6 cut(s) | 387, 735, 1173, 1901, 1944, 2856 |
| Ecl136II | GAGCTC | 1 cut(s) | 1210 |
| Eco130I | CCWWGG | 2 cut(s) | 969, 2121 |
| Eco147I | AGGCCT | 2 cut(s) | 2325, 2773 |
| Eco24I | GRGCYC | 1 cut(s) | 1212 |
| Eco31I | GGTCTC | 1 cut(s) | 1148 |
| Eco32I | GATATC | 1 cut(s) | 2545 |
| Eco47I | GGWCC | 7 cut(s) | 797, 1264, 1429, 1487, 1988, 2045, 2504 |
| Eco53kI | GAGCTC | 1 cut(s) | 1210 |
| Eco57I | CTGAAG | 3 cut(s) | 1584, 2475, 2796 |
| Eco81I | CCTNAGG | 1 cut(s) | 201 |
| Eco88I | CYCGRG | 2 cut(s) | 1090, 1839 |
| Eco91I | GGTNACC | 1 cut(s) | 1319 |
| EcoICRI | GAGCTC | 1 cut(s) | 1210 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2346 |
| EcoO109I | RGGNCCY | 4 cut(s) | 558, 1264, 1487, 2504 |
| EcoO65I | GGTNACC | 1 cut(s) | 1319 |
| EcoRV | GATATC | 1 cut(s) | 2545 |
| EcoT14I | CCWWGG | 2 cut(s) | 969, 2121 |
| EcoT22I | ATGCAT | 1 cut(s) | 1749 |
| EcoT38I | GRGCYC | 1 cut(s) | 1212 |
| ErhI | CCWWGG | 2 cut(s) | 969, 2121 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1837, 1869, 2646, 2678 |
| FaqI | GGGAC | 4 cut(s) | 626, 1500, 1500, 1919 |
| FauI | CCCGC | 2 cut(s) | 2494, 2692 |
| FbaI | TGATCA | 2 cut(s) | 2020, 2098 |
| FblI | GTMKAC | 2 cut(s) | 826, 1569 |
| Fnu4HI | GCNGC | 6 cut(s) | 321, 1000, 1028, 1191, 1310, 1468 |
| FokI | GGATG | 7 cut(s) | 254, 383, 588, 1115, 2138, 2187, 2966 |
| FriOI | GRGCYC | 1 cut(s) | 1212 |
| Fsp4HI | GCNGC | 6 cut(s) | 321, 1000, 1028, 1191, 1310, 1468 |
| FspI | TGCGCA | 1 cut(s) | 2870 |
| GlaI | GCGC | 4 cut(s) | 746, 1712, 2442, 2870 |
| GluI | GCNGC | 6 cut(s) | 321, 1000, 1028, 1191, 1310, 1468 |
| GsuI | CTGGAG | 4 cut(s) | 547, 1121, 1977, 2271 |
| HaeII | RGCGCY | 1 cut(s) | 1714 |
| HaeIII | GGCC | 5 cut(s) | 262, 296, 560, 2325, 2773 |
| HapII | CCGG | 3 cut(s) | 645, 777, 2169 |
| HhaI | GCGC | 4 cut(s) | 747, 1713, 2443, 2871 |
| Hin6I | GCGC | 4 cut(s) | 745, 1711, 2441, 2869 |
| HinP1I | GCGC | 4 cut(s) | 745, 1711, 2441, 2869 |
| HincII | GTYRAC | 3 cut(s) | 505, 699, 2355 |
| HindII | GTYRAC | 3 cut(s) | 505, 699, 2355 |
| HindIII | AAGCTT | 1 cut(s) | 2335 |
| HpaI | GTTAAC | 1 cut(s) | 699 |
| HpaII | CCGG | 3 cut(s) | 645, 777, 2169 |
| HphI | GGTGA | 7 cut(s) | 613, 1546, 1637, 1676, 1825, 2156, 2228 |
| Hpy99I | CGWCG | 1 cut(s) | 1353 |
| HpyCH4III | ACNGT | 4 cut(s) | 927, 1081, 1525, 2960 |
| HpyCH4IV | ACGT | 5 cut(s) | 465, 1790, 1961, 2436, 2851 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 773, 1717, 2113, 2438, 2471, 2519 |
| HpySE526I | ACGT | 5 cut(s) | 465, 1790, 1961, 2436, 2851 |
| HspAI | GCGC | 4 cut(s) | 745, 1711, 2441, 2869 |
| KroI | GCCGGC | 1 cut(s) | 776 |
| KroNI | GCCGGC | 1 cut(s) | 778 |
| Ksp22I | TGATCA | 2 cut(s) | 2020, 2098 |
| KspAI | GTTAAC | 1 cut(s) | 699 |
| Kzo9I | GATC | 8 cut(s) | 70, 220, 1270, 2020, 2098, 2149, 2560, 2780 |
| LguI | GCTCTTC | 2 cut(s) | 735, 1173 |
| LmnI | GCTCC | 2 cut(s) | 2191, 2290 |
| Lsp1109I | GCAGC | 4 cut(s) | 307, 1011, 1177, 1479 |
| LweI | GCATC | 4 cut(s) | 1296, 2102, 2116, 2665 |
| MaeII | ACGT | 5 cut(s) | 465, 1790, 1961, 2436, 2851 |
| MaeIII | GTNAC | 6 cut(s) | 356, 877, 1319, 1664, 1957, 2144 |
| MalI | GATC | 8 cut(s) | 72, 222, 1272, 2022, 2100, 2151, 2562, 2782 |
| MboI | GATC | 8 cut(s) | 70, 220, 1270, 2020, 2098, 2149, 2560, 2780 |
| MhlI | GDGCHC | 3 cut(s) | 189, 1212, 2746 |
| MlsI | TGGCCA | 1 cut(s) | 262 |
| MluNI | TGGCCA | 1 cut(s) | 262 |
| MmeI | TCCRAC | 5 cut(s) | 135, 834, 1080, 2072, 2703 |
| Mox20I | TGGCCA | 1 cut(s) | 262 |
| Mph1103I | ATGCAT | 1 cut(s) | 1749 |
| MroNI | GCCGGC | 1 cut(s) | 776 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2384 |
| MscI | TGGCCA | 1 cut(s) | 262 |
| Msp20I | TGGCCA | 1 cut(s) | 262 |
| MspI | CCGG | 3 cut(s) | 645, 777, 2169 |
| MspR9I | CCNGG | 1 cut(s) | 645 |
| Mva1269I | GAATGC | 1 cut(s) | 1879 |
| MvnI | CGCG | 1 cut(s) | 747 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 773, 1717, 2113, 2438, 2471, 2519 |
| NaeI | GCCGGC | 1 cut(s) | 778 |
| NciI | CCSGG | 1 cut(s) | 645 |
| NcoI | CCATGG | 1 cut(s) | 2121 |
| NdeII | GATC | 8 cut(s) | 70, 220, 1270, 2020, 2098, 2149, 2560, 2780 |
| NgoMIV | GCCGGC | 1 cut(s) | 776 |
| NlaIV | GGNNCC | 5 cut(s) | 559, 948, 1488, 2046, 2505 |
| NmuCI | GTSAC | 3 cut(s) | 356, 1664, 2144 |
| NsbI | TGCGCA | 1 cut(s) | 2870 |
| NsiI | ATGCAT | 1 cut(s) | 1749 |
| NspI | RCATGY | 1 cut(s) | 2285 |
| PaeI | GCATGC | 1 cut(s) | 2285 |
| PagI | TCATGA | 2 cut(s) | 2239, 2388 |
| PaqCI | CACCTGC | 1 cut(s) | 1652 |
| PceI | AGGCCT | 2 cut(s) | 2325, 2773 |
| PciSI | GCTCTTC | 2 cut(s) | 735, 1173 |
| PctI | GAATGC | 1 cut(s) | 1879 |
| PdiI | GCCGGC | 1 cut(s) | 778 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2384 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 494 |
| PkrI | GCNGC | 6 cut(s) | 322, 1001, 1029, 1192, 1311, 1469 |
| PpuMI | RGGWCCY | 3 cut(s) | 1264, 1487, 2504 |
| PshBI | ATTAAT | 1 cut(s) | 2133 |
| PsiI | TTATAA | 1 cut(s) | 2061 |
| Psp124BI | GAGCTC | 1 cut(s) | 1212 |
| Psp5II | RGGWCCY | 3 cut(s) | 1264, 1487, 2504 |
| PspEI | GGTNACC | 1 cut(s) | 1319 |
| PspN4I | GGNNCC | 5 cut(s) | 559, 948, 1488, 2046, 2505 |
| PspPI | GGNCC | 8 cut(s) | 558, 797, 1264, 1429, 1487, 1988, 2045, 2504 |
| PspPPI | RGGWCCY | 3 cut(s) | 1264, 1487, 2504 |
| PstI | CTGCAG | 1 cut(s) | 2817 |
| PstNI | CAGNNNCTG | 1 cut(s) | 320 |
| RsaI | GTAC | 8 cut(s) | 980, 1404, 1527, 1582, 1852, 2253, 2621, 2934 |
| RsaNI | GTAC | 8 cut(s) | 979, 1403, 1526, 1581, 1851, 2252, 2620, 2933 |
| SacI | GAGCTC | 1 cut(s) | 1212 |
| SapI | GCTCTTC | 2 cut(s) | 735, 1173 |
| SatI | GCNGC | 6 cut(s) | 321, 1000, 1028, 1191, 1310, 1468 |
| Sau3AI | GATC | 8 cut(s) | 70, 220, 1270, 2020, 2098, 2149, 2560, 2780 |
| Sau96I | GGNCC | 8 cut(s) | 558, 797, 1264, 1429, 1487, 1988, 2045, 2504 |
| ScrFI | CCNGG | 1 cut(s) | 645 |
| SduI | GDGCHC | 3 cut(s) | 189, 1212, 2746 |
| SfaNI | GCATC | 4 cut(s) | 1296, 2102, 2116, 2665 |
| SfcI | CTRYAG | 2 cut(s) | 1194, 2813 |
| SinI | GGWCC | 7 cut(s) | 797, 1264, 1429, 1487, 1988, 2045, 2504 |
| SmlI | CTYRAG | 5 cut(s) | 236, 2003, 2319, 2855, 2877 |
| SmoI | CTYRAG | 5 cut(s) | 236, 2003, 2319, 2855, 2877 |
| SpeI | ACTAGT | 1 cut(s) | 2357 |
| SphI | GCATGC | 1 cut(s) | 2285 |
| SseBI | AGGCCT | 2 cut(s) | 2325, 2773 |
| SsiI | CCGC | 5 cut(s) | 1028, 1309, 1427, 2501, 2699 |
| SspI | AATATT | 1 cut(s) | 1022 |
| SstI | GAGCTC | 1 cut(s) | 1212 |
| StuI | AGGCCT | 2 cut(s) | 2325, 2773 |
| StyD4I | CCNGG | 1 cut(s) | 643 |
| StyI | CCWWGG | 2 cut(s) | 969, 2121 |
| TaaI | ACNGT | 4 cut(s) | 927, 1081, 1525, 2960 |
| TaiI | ACGT | 5 cut(s) | 468, 1793, 1964, 2439, 2854 |
| TaqI | TCGA | 7 cut(s) | 27, 219, 1364, 1596, 2137, 2395, 2563 |
| TatI | WGTACW | 6 cut(s) | 978, 1402, 1525, 2251, 2619, 2932 |
| TauI | GCSGC | 2 cut(s) | 1030, 1312 |
| TscAI | CASTG | 7 cut(s) | 571, 1086, 1190, 1255, 1285, 2467, 2798 |
| TseFI | GTSAC | 3 cut(s) | 356, 1664, 2144 |
| TseI | GCWGC | 4 cut(s) | 320, 999, 1190, 1467 |
| Tsp45I | GTSAC | 3 cut(s) | 356, 1664, 2144 |
| TspGWI | ACGGA | 1 cut(s) | 2040 |
| TspRI | CASTG | 7 cut(s) | 571, 1086, 1190, 1255, 1285, 2467, 2798 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 494 |
| VneI | GTGCAC | 2 cut(s) | 185, 2742 |
| VpaK11BI | GGWCC | 7 cut(s) | 797, 1264, 1429, 1487, 1988, 2045, 2504 |
| VspI | ATTAAT | 1 cut(s) | 2133 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2346 |
| XapI | RAATTY | 5 cut(s) | 1231, 1739, 2484, 2802, 2890 |
| XceI | RCATGY | 1 cut(s) | 2285 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1504 |
| XmiI | GTMKAC | 2 cut(s) | 826, 1569 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2384 |
| Zsp2I | ATGCAT | 1 cut(s) | 1749 |