Rmu_sc0001563.1_g000043

Cell division cycle protein 48 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001563.1
OG0000153
Physical Location & Seq
Reverse (-)
140562 .. 144156
3595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001563.1_g000043.1.cds

Sequence Viewer

Length: 2136 bp
atgttagacaatgtgaataatttcatctggcagccctatctccaaccttctgagtttgagaccttttcgttttctggtagggaagagcaatgtgctatagctctcggtcgggttgagctatacatgactgtttgcccaaattttatcccatccatcagtgaggataaggaagacttggatattttccctgttgtttattctcctgacagagtgatgcggcaacttggctgccttcaggatgttccctcacctccagctcgctcccttaactctaagatcacaagcttttcccatatgaagcgtagatgcgtacttgacttctctggcagaattgcggacttcccatctcagttgtcagggaacgccatgaatgctaacgcctcccctgcttatagttgttattggaggggggtgttgtgcaggttcaggctttttgtggaagccggcggtcctcagctaaagttgggacctattaccagcaataggttggctagccccaaggctgtattgacttatgctgaaaagcataacctcacgtataccgaagtgtacagcgatggtactagaaaggttgtcggctatttaaagggttttgaagctaagagggtgaagggagaggaaaagaagtgcgataaggagataggtgataagggtgatgcgagggaacacaacactcagaagcctattcggcgtttcaagcccaaggtggattttgacaagtttatgcatacgacccaacacggagtcaaactgatgatccccccgagcatgccacttcaaaagaacaaggaggtaaagaagaaaagatcttcaacaccccgagcaggtggtacttctcatgcaaagcaagcgaatgctaccaatttcgaagtgaagaaaagggtgaagagggaggctggtgcagattttgatgtccctcttcgtcgaagcaaccatgtgaagtccaagagttctccttcccctcgtggtcctccagcttatcttgagttatctgaatatgatcacggagagggttctcaagggggtccaaccgattcttctaaagaggatcatactaacccttcaaaaggggcatctgatcatctgcctgaagtgcctcccatgattagccaagatagtgacaatcaggttatcgaagatgaaggcatgagtgatggtgataaggtcgtcgaagatgaaggcatgagtgatggtgataaggttgtcgaagatgaaggcatgaatgatggtgataaggttgtcgaagatggaggcatgagtgatgccgctgtagttagtgatgatactgttttgatactgctgctattacatgagttttgcccctttgatgatgtgaaatgtgaaaggcgtggtcatccttcggatgttggtaagagtaatgacgaggttggtcatgacacggcagtacatgccttagttgagtctgataagcacactccagggattgataaggtgactatcattcaaacgcaaagccttggcccaagcttgcctgctaaagggtcaactagtgagtgggtgacccatatggacaactttatgattcgatttgcttctaaagaggagtctgctctaagcatggctgacgttacaccagtgttgagcggagacatcgctgtttttgcgggtatgaaaattccttcagagttagtgggtcccttagctagatttgctgagagatatagcggtggagatatctttagtttaattgatcgagactatacttcaaggaagaagtgtgagttgattaaggaacttggcatgatgttgtacagtatggaagtttgccagataaaagatggagaagtattcctcatttggagagacacctgtcgtgatttcatgggggcaggtcttaatgttggcttcttgttggattccttaaaacaagatgcaaagcatttcttttgttatatgatgaggtctgaaggatcagagaaccagtatgttaaagttcaaggtctgcaggagatggttttgcaaaccaagaaggagcttgcacgtcttgagggagagttgcgcaaggctcaagatgatctgagttccattttgcgtgacgtttctgtgcctgccatggagtgcgtactagaatcttcttggaaggacggtagtcacttctcttattatttatattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
CDC48_2 CDC48_N Vps4_C

Protein Analysis

711

Amino Acids

79.2

Weight (kDa)

5.58

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000153)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25700 FvH4_2g11190 FvH4_2g11201 FvH4_2g16130 FvH4_3g40630 FvH4_4g02210 FvH4_4g14564 FvH4_6g24041 FvH4_6g24050 FvH4_6g24050
rosa_chinensis RchiOBHm_Chr1g0348241 RchiOBHm_Chr2g0109211 RchiOBHm_Chr2g0144471 RchiOBHm_Chr2g0174771 RchiOBHm_Chr3g0495101 RchiOBHm_Chr5g0047301 RchiOBHm_Chr5g0062201
rosa_laevigata RLG00000000570 RLG00000000571 RLG00000008718 RLG00000008719 RLG00000008835 RLG00000019314 RLG00000019316 RLG00000020979
rosa_multiflora Rmu_co8024416.1_g000001 Rmu_co8152282.1_g000001 Rmu_sc0000103.1_g000058 Rmu_sc0000161.1_g000022 Rmu_sc0000517.1_g000009 Rmu_sc0000517.1_g000010 Rmu_sc0000571.1_g000024 Rmu_sc0000580.1_g000130 Rmu_sc0000744.1_g000130 Rmu_sc0000796.1_g000017 Rmu_sc0000800.1_g000021 Rmu_sc0000830.1_g000037 Rmu_sc0000830.1_g000038 Rmu_sc0000830.1_g000059 Rmu_sc0000958.1_g000012 Rmu_sc0001094.1_g000018 Rmu_sc0001163.1_g000004 Rmu_sc0001200.1_g000052 Rmu_sc0001339.1_g000005 Rmu_sc0001397.1_g000009 Rmu_sc0001563.1_g000043 Rmu_sc0001563.1_g000044 Rmu_sc0001570.1_g000001 Rmu_sc0001629.1_g000016 Rmu_sc0001763.1_g000006 Rmu_sc0002011.1_g000013 Rmu_sc0002012.1_g000012 Rmu_sc0002268.1_g000026 Rmu_sc0002268.1_g000027 Rmu_sc0002321.1_g000021 Rmu_sc0002722.1_g000002 Rmu_sc0003032.1_g000006 Rmu_sc0003185.1_g000008 Rmu_sc0003238.1_g000006 Rmu_sc0003275.1_g000037 Rmu_sc0003275.1_g000038 Rmu_sc0003441.1_g000042 Rmu_sc0003748.1_g000027 Rmu_sc0004229.1_g000011 Rmu_sc0004283.1_g000041 Rmu_sc0004283.1_g000042 Rmu_sc0004283.1_g000043 Rmu_sc0004841.1_g000026 Rmu_sc0004841.1_g000031 Rmu_sc0004877.1_g000001 Rmu_sc0005039.1_g000012 Rmu_sc0005584.1_g000019 Rmu_sc0005584.1_g000020 Rmu_sc0005584.1_g000021 Rmu_sc0005600.1_g000021 Rmu_sc0005914.1_g000004 Rmu_sc0006139.1_g000006 Rmu_sc0006638.1_g000001 Rmu_sc0007228.1_g000001 Rmu_sc0007300.1_g000008 Rmu_sc0007486.1_g000002 Rmu_sc0007737.1_g000007 Rmu_sc0008328.1_g000015 Rmu_sc0009064.1_g000002 Rmu_sc0009315.1_g000006 Rmu_sc0009472.1_g000009 Rmu_sc0009774.1_g000016 Rmu_sc0009858.1_g000010 Rmu_sc0009858.1_g000011 Rmu_sc0010489.1_g000001 Rmu_sc0010579.1_g000002 Rmu_sc0011511.1_g000002 Rmu_sc0013359.1_g000001 Rmu_sc0014367.1_g000005 Rmu_sc0015624.1_g000003 Rmu_sc0016756.1_g000001 Rmu_sc0021286.1_g000001 Rmu_sc0021693.1_g000001 Rmu_sc0022538.1_g000001 Rmu_sc0030983.1_g000001 Rmu_sc0037682.1_g000001 Rmu_sc0041059.1_g000001 Rmu_ssc0000003.1_g000013 Rmu_ssc0000003.1_g000015 Rmu_ssc0000388.1_g000018 Rmu_ssc0000409.1_g000013 Rmu_ssc0000409.1_g000015 Rmu_ssc0000476.1_g000003
rosa_roxburghii Rroxscaffold_1G00017510 Rroxscaffold_1G00017520 Rroxscaffold_1G00017530 Rroxscaffold_1G00017990 Rroxscaffold_1G00031430 Rroxscaffold_1G00045790 Rroxscaffold_2G00108360 Rroxscaffold_2G00127910 Rroxscaffold_3G00218970 Rroxscaffold_3G00218980 Rroxscaffold_3G00218990 Rroxscaffold_3G00226450 Rroxscaffold_3G00226460 Rroxscaffold_3G00230570 Rroxscaffold_3G00230920 Rroxscaffold_4G00284360 Rroxscaffold_4G00292370 Rroxscaffold_4G00307090 Rroxscaffold_4G00319260 Rroxscaffold_4G00332580 Rroxscaffold_5G00341080 Rroxscaffold_5G00344620 Rroxscaffold_5G00358950 Rroxscaffold_5G00368600 Rroxscaffold_5G00368610 Rroxscaffold_5G00378350 Rroxscaffold_6G00388550 Rroxscaffold_6G00398560 Rroxscaffold_6G00398570 Rroxscaffold_7G00194900 Rroxscaffold_7G00194910 Rroxscaffold_7G00194920
rosa_rugosa Rorug05G0347200 Rorug06G0064400 Rorug06G0064500 Rorug07G0190700 Rorug07G0190800 Rorug07G0276200 Rorug07G0344800 Rorug07G0344900
rosa_samantha Rh1AG138000 Rh1AG138100 Rh1DG079100 Rh1DG264500 Rh1DG264600 Rh1DG275600 Rh1DG275700 Rh2AG474800 Rh2AG474900 Rh2AG557100 Rh2AG557200 Rh2AG567400 Rh2AG568000 Rh2AG568100 Rh3CG345000 Rh3CG351000 Rh3CG364300 Rh4AG186000 Rh5AG278000 Rh5DG280000 Rh5DG281700 Rh5DG447800 Rh7AG499400 Rh7BG470300 Rh7BG470400 Rh7DG197800 Rh7DG197900 Rh7DG198000 Rh7DG284800 Rh7DG311600 Rh7DG311700 Rh7DG329300 Rh7DG393300 Rh7DG393500 Rh7DG483800 Rh7DG483900
rosa_wichuraiana Rw0G003510 Rw1G021670 Rw2G016920 Rw3G020700 Rw7G028090 Rw7G031160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 815
Acc16I TGCGCA 1 cut(s) 2021
Acc36I ACCTGC 3 cut(s) 411, 815, 1841
AccBSI CCGCTC 1 cut(s) 1605
AccI GTMKAC 1 cut(s) 539
AciI CCGC 7 cut(s) 217, 335, 447, 1266, 1605, 1625, 1686
AclWI GGATC 3 cut(s) 751, 1056, 1939
AcsI RAATTY 2 cut(s) 139, 1635
AcuI CTGAAG 4 cut(s) 218, 1110, 1626, 1947
AfaI GTAC 7 cut(s) 312, 551, 562, 832, 1407, 1772, 2085
AfiI CCNNNNNNNGG 4 cut(s) 483, 824, 1067, 1499
AgsI TTSAA 8 cut(s) 596, 697, 779, 813, 1065, 1466, 1728, 1958
AhlI ACTAGT 1 cut(s) 1508
AjiI CACGTC 1 cut(s) 2003
AjnI CCWGG 1 cut(s) 1438
AjuI GAANNNNNNNTTGG 2 cut(s) 1021, 1053
Alw26I GTCTC 4 cut(s) 53, 1602, 1710, 1818
AlwI GGATC 3 cut(s) 751, 1056, 1939
Ama87I CYCGRG 2 cut(s) 763, 819
AoxI GGCC 1 cut(s) 1480
ApeKI GCWGC 3 cut(s) 31, 228, 1300
ApoI RAATTY 2 cut(s) 139, 1635
Asp700I GAANNNNTTC 1 cut(s) 20
AspLEI GCGC 1 cut(s) 2022
AspS9I GGNCC 6 cut(s) 449, 467, 968, 1025, 1481, 1655
AsuII TTCGAA 1 cut(s) 867
AsuNHI GCTAGC 1 cut(s) 491
AvaI CYCGRG 2 cut(s) 763, 819
AvaII GGWCC 5 cut(s) 449, 467, 968, 1025, 1655
BauI CACGAG 1 cut(s) 963
BbsI GAAGAC 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 3 cut(s) 43, 215, 1287
BceAI ACGGC 1 cut(s) 1416
BciT130I CCWGG 1 cut(s) 1440
BclI TGATCA 2 cut(s) 1000, 1078
BcoDI GTCTC 4 cut(s) 53, 1602, 1710, 1818
BcuI ACTAGT 1 cut(s) 1508
BfaI CTAG 5 cut(s) 492, 564, 1509, 1665, 2087
BfmI CTRYAG 3 cut(s) 96, 1269, 1964
BfuAI ACCTGC 3 cut(s) 411, 815, 1841
BglI GCCNNNNNGGC 1 cut(s) 688
BglII AGATCT 1 cut(s) 806
BisI GCNGC 5 cut(s) 32, 218, 229, 1266, 1301
BlsI GCNGC 5 cut(s) 33, 219, 230, 1267, 1302
Bme1390I CCNGG 1 cut(s) 1440
Bme18I GGWCC 5 cut(s) 449, 467, 968, 1025, 1655
BmeT110I CYCGRG 2 cut(s) 763, 819
BmgBI CACGTC 1 cut(s) 2003
BmgT120I GGNCC 6 cut(s) 449, 467, 968, 1025, 1481, 1655
BmiI GGNNCC 4 cut(s) 468, 1026, 1656, 1657
BmrFI CCNGG 1 cut(s) 1440
BmsI GCATC 6 cut(s) 204, 296, 646, 1082, 1252, 1882
BmtI GCTAGC 1 cut(s) 495
BoxI GACNNNNGTC 2 cut(s) 1830, 2109
BpiI GAAGAC 1 cut(s) 177
BpmI CTGGAG 3 cut(s) 237, 957, 1422
Bpu10I CCTNAGC 2 cut(s) 453, 1660
Bpu14I TTCGAA 1 cut(s) 867
BpuEI CTTGAG 4 cut(s) 1002, 1004, 2013, 2027
BsaAI YACGTR 1 cut(s) 537
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 5 cut(s) 498, 702, 1439, 1477, 2073
Bsc4I CCNNNNNNNGG 4 cut(s) 483, 824, 1067, 1499
Bse118I RCCGGY 1 cut(s) 443
Bse1I ACTGG 2 cut(s) 1595, 1942
Bse3DI GCAATG 1 cut(s) 95
BseBI CCWGG 1 cut(s) 1440
BseDI CCNNGG 5 cut(s) 498, 702, 1439, 1477, 2073
BseGI GGATG 4 cut(s) 149, 244, 1354, 1369
BseLI CCNNNNNNNGG 4 cut(s) 483, 824, 1067, 1499
BseMI GCAATG 1 cut(s) 95
BseMII CTCAG 6 cut(s) 42, 362, 467, 689, 1665, 2030
BseNI ACTGG 2 cut(s) 1595, 1942
BseRI GAGGAG 1 cut(s) 1577
BseXI GCAGC 3 cut(s) 43, 215, 1287
BsgI GTGCAG 2 cut(s) 439, 921
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 1 cut(s) 1482
BsiEI CGRYCG 1 cut(s) 109
BsiHKCI CYCGRG 2 cut(s) 763, 819
BsiSI CCGG 1 cut(s) 444
BslFI GGGAC 3 cut(s) 480, 899, 1641
BslI CCNNNNNNNGG 4 cut(s) 483, 824, 1067, 1499
BsmAI GTCTC 4 cut(s) 53, 1602, 1710, 1818
BsmFI GGGAC 3 cut(s) 480, 899, 1641
BsmI GAATGC 2 cut(s) 376, 859
BsnI GGCC 1 cut(s) 1482
Bso31I GGTCTC 1 cut(s) 53
BsoBI CYCGRG 2 cut(s) 763, 819
Bsp119I TTCGAA 1 cut(s) 867
Bsp1407I TGTACA 2 cut(s) 549, 1770
Bsp143I GATC 9 cut(s) 276, 756, 806, 1000, 1048, 1078, 1711, 1931, 2035
Bsp19I CCATGG 1 cut(s) 2073
BspACI CCGC 7 cut(s) 217, 335, 447, 1266, 1605, 1625, 1686
BspANI GGCC 1 cut(s) 1482
BspCNI CTCAG 6 cut(s) 43, 361, 466, 688, 1666, 2031
BspHI TCATGA 1 cut(s) 1393
BspLI GGNNCC 4 cut(s) 468, 1026, 1656, 1657
BspMAI CTGCAG 1 cut(s) 1968
BspMI ACCTGC 3 cut(s) 411, 815, 1841
BspOI GCTAGC 1 cut(s) 495
BspPI GGATC 3 cut(s) 751, 1056, 1939
BspQI GCTCTTC 1 cut(s) 78
BspT104I TTCGAA 1 cut(s) 867
BspTNI GGTCTC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 1605
BsrDI GCAATG 1 cut(s) 95
BsrFI RCCGGY 1 cut(s) 443
BsrGI TGTACA 2 cut(s) 549, 1770
BsrI ACTGG 2 cut(s) 1595, 1942
BssAI RCCGGY 1 cut(s) 443
BssECI CCNNGG 5 cut(s) 498, 702, 1439, 1477, 2073
BssMI GATC 9 cut(s) 276, 756, 806, 1000, 1048, 1078, 1711, 1931, 2035
BssNAI GTATAC 1 cut(s) 540
BssSI CACGAG 1 cut(s) 963
BssT1I CCWWGG 4 cut(s) 498, 702, 1477, 2073
Bst1107I GTATAC 1 cut(s) 540
Bst2BI CACGAG 1 cut(s) 963
Bst2UI CCWGG 1 cut(s) 1440
Bst4CI ACNGT 4 cut(s) 130, 1288, 1775, 2108
Bst6I CTCTTC 3 cut(s) 78, 881, 924
BstAPI GCANNNNNTGC 1 cut(s) 1409
BstAUI TGTACA 2 cut(s) 549, 1770
BstBAI YACGTR 1 cut(s) 537
BstBI TTCGAA 1 cut(s) 867
BstC8I GCNNGC 9 cut(s) 259, 445, 493, 770, 849, 1490, 1494, 1998, 2070
BstDSI CCRYGG 1 cut(s) 2073
BstEII GGTNACC 1 cut(s) 1519
BstENI CCTNNNNNAGG 2 cut(s) 1065, 1497
BstF5I GGATG 4 cut(s) 149, 244, 1354, 1369
BstHHI GCGC 1 cut(s) 2022
BstKTI GATC 9 cut(s) 279, 759, 809, 1003, 1051, 1081, 1714, 1934, 2038
BstMAI GTCTC 4 cut(s) 53, 1602, 1710, 1818
BstMBI GATC 9 cut(s) 276, 756, 806, 1000, 1048, 1078, 1711, 1931, 2035
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 9 cut(s) 371, 386, 688, 697, 848, 1093, 1409, 1622, 1670
BstNI CCWGG 1 cut(s) 1440
BstNSI RCATGY 2 cut(s) 772, 1412
BstPAI GACNNNNGTC 2 cut(s) 1830, 2109
BstPI GGTNACC 1 cut(s) 1519
BstSCI CCNGG 1 cut(s) 1438
BstSFI CTRYAG 3 cut(s) 96, 1269, 1964
BstV1I GCAGC 3 cut(s) 43, 215, 1287
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 806
BstYI RGATCY 1 cut(s) 806
BstZ17I GTATAC 1 cut(s) 540
BsuRI GGCC 1 cut(s) 1482
BtgI CCRYGG 1 cut(s) 2073
BtgZI GCGATG 2 cut(s) 570, 1597
BtrI CACGTC 1 cut(s) 2003
BtsCI GGATG 4 cut(s) 149, 244, 1354, 1369
BtsIMutI CAGTG 2 cut(s) 163, 1602
BveI ACCTGC 3 cut(s) 411, 815, 1841
Cac8I GCNNGC 9 cut(s) 259, 445, 493, 770, 849, 1490, 1494, 1998, 2070
CciI TCATGA 1 cut(s) 1393
CfoI GCGC 1 cut(s) 2022
Cfr10I RCCGGY 1 cut(s) 443
Cfr13I GGNCC 6 cut(s) 449, 467, 968, 1025, 1481, 1655
Csp6I GTAC 7 cut(s) 311, 550, 561, 831, 1406, 1771, 2084
CviQI GTAC 7 cut(s) 311, 550, 561, 831, 1406, 1771, 2084
DpnI GATC 9 cut(s) 278, 758, 808, 1002, 1050, 1080, 1713, 1933, 2037
DpnII GATC 9 cut(s) 276, 756, 806, 1000, 1048, 1078, 1711, 1931, 2035
DraI TTTAAA 1 cut(s) 585
Eam1104I CTCTTC 3 cut(s) 78, 881, 924
EarI CTCTTC 3 cut(s) 78, 881, 924
Eco130I CCWWGG 4 cut(s) 498, 702, 1477, 2073
Eco31I GGTCTC 1 cut(s) 53
Eco32I GATATC 1 cut(s) 1696
Eco47I GGWCC 5 cut(s) 449, 467, 968, 1025, 1655
Eco57I CTGAAG 4 cut(s) 218, 1110, 1626, 1947
Eco88I CYCGRG 2 cut(s) 763, 819
Eco91I GGTNACC 1 cut(s) 1519
EcoNI CCTNNNNNAGG 2 cut(s) 1065, 1497
EcoO109I RGGNCCY 2 cut(s) 467, 1655
EcoO65I GGTNACC 1 cut(s) 1519
EcoRII CCWGG 1 cut(s) 1438
EcoRV GATATC 1 cut(s) 1696
EcoT14I CCWWGG 4 cut(s) 498, 702, 1477, 2073
EcoT22I ATGCAT 1 cut(s) 729
ErhI CCWWGG 4 cut(s) 498, 702, 1477, 2073
FalI AAGNNNNNCTT 4 cut(s) 158, 190, 1889, 1921
FaqI GGGAC 3 cut(s) 480, 899, 1641
FauI CCCGC 1 cut(s) 1618
FauNDI CATATG 2 cut(s) 294, 1527
FbaI TGATCA 2 cut(s) 1000, 1078
FblI GTMKAC 1 cut(s) 539
Fnu4HI GCNGC 5 cut(s) 32, 218, 229, 1266, 1301
FokI GGATG 4 cut(s) 136, 251, 1341, 1376
Fsp4HI GCNGC 5 cut(s) 32, 218, 229, 1266, 1301
FspBI CTAG 5 cut(s) 492, 564, 1509, 1665, 2087
FspI TGCGCA 1 cut(s) 2021
GlaI GCGC 1 cut(s) 2021
GluI GCNGC 5 cut(s) 32, 218, 229, 1266, 1301
GsuI CTGGAG 3 cut(s) 237, 957, 1422
HaeIII GGCC 1 cut(s) 1482
HapII CCGG 1 cut(s) 444
HhaI GCGC 1 cut(s) 2022
Hin6I GCGC 1 cut(s) 2020
HinP1I GCGC 1 cut(s) 2020
HincII GTYRAC 1 cut(s) 1506
HindII GTYRAC 1 cut(s) 1506
HindIII AAGCTT 2 cut(s) 283, 1486
HinfI GANTC 7 cut(s) 744, 1034, 1421, 1543, 1565, 1877, 2090
HpaII CCGG 1 cut(s) 444
Hpy166II GTNNAC 3 cut(s) 540, 550, 1506
Hpy188III TCNNGA 8 cut(s) 203, 236, 983, 1394, 1715, 1835, 2006, 2030
Hpy8I GTNNAC 3 cut(s) 540, 550, 1506
Hpy99I CGWCG 2 cut(s) 927, 1172
HpyCH4III ACNGT 4 cut(s) 130, 1288, 1775, 2108
HpyCH4IV ACGT 4 cut(s) 536, 1587, 2002, 2058
HpyCH4V TGCA 8 cut(s) 420, 727, 842, 902, 1895, 1966, 1981, 2000
HpyF10VI GCNNNNNNNGC 9 cut(s) 371, 386, 688, 697, 848, 1093, 1409, 1622, 1670
HpySE526I ACGT 4 cut(s) 536, 1587, 2002, 2058
HspAI GCGC 1 cut(s) 2020
KflI GGGWCCC 1 cut(s) 1655
KroI GCCGGC 1 cut(s) 443
KroNI GCCGGC 1 cut(s) 445
Ksp22I TGATCA 2 cut(s) 1000, 1078
Kzo9I GATC 9 cut(s) 276, 756, 806, 1000, 1048, 1078, 1711, 1931, 2035
LguI GCTCTTC 1 cut(s) 78
LmnI GCTCC 2 cut(s) 266, 1993
Lsp1109I GCAGC 3 cut(s) 43, 215, 1287
LweI GCATC 6 cut(s) 204, 296, 646, 1082, 1252, 1882
MaeI CTAG 5 cut(s) 492, 564, 1509, 1665, 2087
MaeII ACGT 4 cut(s) 536, 1587, 2002, 2058
MaeIII GTNAC 6 cut(s) 1118, 1453, 1519, 1588, 2054, 2111
MalI GATC 9 cut(s) 278, 758, 808, 1002, 1050, 1080, 1713, 1933, 2037
MbiI CCGCTC 1 cut(s) 1605
MboI GATC 9 cut(s) 276, 756, 806, 1000, 1048, 1078, 1711, 1931, 2035
MflI RGATCY 1 cut(s) 806
MluCI AATT 6 cut(s) 19, 139, 330, 862, 1635, 1707
MlyI GAGTC 3 cut(s) 753, 1430, 1574
MmeI TCCRAC 3 cut(s) 67, 1052, 1854
Mph1103I ATGCAT 1 cut(s) 729
MroNI GCCGGC 1 cut(s) 443
MroXI GAANNNNTTC 1 cut(s) 20
MseI TTAA 7 cut(s) 267, 584, 1706, 1749, 1857, 1883, 1950
MspA1I CMGCKG 1 cut(s) 1268
MspI CCGG 1 cut(s) 444
MspR9I CCNGG 1 cut(s) 1440
Mva1269I GAATGC 2 cut(s) 376, 859
MvaI CCWGG 1 cut(s) 1440
MwoI GCNNNNNNNGC 9 cut(s) 371, 386, 688, 697, 848, 1093, 1409, 1622, 1670
NaeI GCCGGC 1 cut(s) 445
NcoI CCATGG 1 cut(s) 2073
NdeI CATATG 2 cut(s) 294, 1527
NdeII GATC 9 cut(s) 276, 756, 806, 1000, 1048, 1078, 1711, 1931, 2035
NgoMIV GCCGGC 1 cut(s) 443
NheI GCTAGC 1 cut(s) 491
NlaIV GGNNCC 4 cut(s) 468, 1026, 1656, 1657
NmuCI GTSAC 5 cut(s) 1118, 1453, 1519, 2054, 2111
NsbI TGCGCA 1 cut(s) 2021
NsiI ATGCAT 1 cut(s) 729
NspI RCATGY 2 cut(s) 772, 1412
NspV TTCGAA 1 cut(s) 867
PaeI GCATGC 1 cut(s) 772
PagI TCATGA 1 cut(s) 1393
PaqCI CACCTGC 1 cut(s) 815
PciSI GCTCTTC 1 cut(s) 78
PctI GAATGC 2 cut(s) 376, 859
PdiI GCCGGC 1 cut(s) 445
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 4 cut(s) 1034, 1543, 1877, 2090
PkrI GCNGC 5 cut(s) 33, 219, 230, 1267, 1302
PleI GAGTC 3 cut(s) 752, 1429, 1573
PpsI GAGTC 3 cut(s) 752, 1429, 1573
Ppu21I YACGTR 1 cut(s) 537
PpuMI RGGWCCY 2 cut(s) 467, 1655
PshAI GACNNNNGTC 2 cut(s) 1830, 2109
Psp5II RGGWCCY 2 cut(s) 467, 1655
Psp6I CCWGG 1 cut(s) 1438
PspEI GGTNACC 1 cut(s) 1519
PspGI CCWGG 1 cut(s) 1438
PspN4I GGNNCC 4 cut(s) 468, 1026, 1656, 1657
PspPI GGNCC 6 cut(s) 449, 467, 968, 1025, 1481, 1655
PspPPI RGGWCCY 2 cut(s) 467, 1655
PstI CTGCAG 1 cut(s) 1968
PsuI RGATCY 1 cut(s) 806
RsaI GTAC 7 cut(s) 312, 551, 562, 832, 1407, 1772, 2085
RsaNI GTAC 7 cut(s) 311, 550, 561, 831, 1406, 1771, 2084
SapI GCTCTTC 1 cut(s) 78
SaqAI TTAA 7 cut(s) 267, 584, 1706, 1749, 1857, 1883, 1950
SatI GCNGC 5 cut(s) 32, 218, 229, 1266, 1301
Sau3AI GATC 9 cut(s) 276, 756, 806, 1000, 1048, 1078, 1711, 1931, 2035
Sau96I GGNCC 6 cut(s) 449, 467, 968, 1025, 1481, 1655
SchI GAGTC 3 cut(s) 753, 1430, 1574
ScrFI CCNGG 1 cut(s) 1440
SfaNI GCATC 6 cut(s) 204, 296, 646, 1082, 1252, 1882
SfcI CTRYAG 3 cut(s) 96, 1269, 1964
SfuI TTCGAA 1 cut(s) 867
SinI GGWCC 5 cut(s) 449, 467, 968, 1025, 1655
SmlI CTYRAG 4 cut(s) 983, 1017, 2006, 2028
SmoI CTYRAG 4 cut(s) 983, 1017, 2006, 2028
SpeI ACTAGT 1 cut(s) 1508
SphI GCATGC 1 cut(s) 772
Sse9I AATT 6 cut(s) 19, 139, 330, 862, 1635, 1707
SsiI CCGC 7 cut(s) 217, 335, 447, 1266, 1605, 1625, 1686
SspMI CTAG 5 cut(s) 492, 564, 1509, 1665, 2087
StyD4I CCNGG 1 cut(s) 1438
StyI CCWWGG 4 cut(s) 498, 702, 1477, 2073
TaaI ACNGT 4 cut(s) 130, 1288, 1775, 2108
TaiI ACGT 4 cut(s) 539, 1590, 2005, 2061
TaqI TCGA 8 cut(s) 867, 925, 1134, 1170, 1206, 1242, 1546, 1714
TaqII GACCGA 1 cut(s) 95
TasI AATT 6 cut(s) 19, 139, 330, 862, 1635, 1707
TatI WGTACW 3 cut(s) 549, 1405, 1770
TauI GCSGC 2 cut(s) 220, 1268
TfiI GAWTC 4 cut(s) 1034, 1543, 1877, 2090
Tru1I TTAA 7 cut(s) 267, 584, 1706, 1749, 1857, 1883, 1950
Tru9I TTAA 7 cut(s) 267, 584, 1706, 1749, 1857, 1883, 1950
TscAI CASTG 2 cut(s) 163, 1602
TseFI GTSAC 5 cut(s) 1118, 1453, 1519, 2054, 2111
TseI GCWGC 3 cut(s) 31, 228, 1300
Tsp45I GTSAC 5 cut(s) 1118, 1453, 1519, 2054, 2111
TspDTI ATGAA 9 cut(s) 13, 311, 383, 1155, 1191, 1227, 1235, 1646, 1831
TspGWI ACGGA 2 cut(s) 756, 1020
TspRI CASTG 2 cut(s) 163, 1602
VpaK11BI GGWCC 5 cut(s) 449, 467, 968, 1025, 1655
XagI CCTNNNNNAGG 2 cut(s) 1065, 1497
XapI RAATTY 2 cut(s) 139, 1635
XceI RCATGY 2 cut(s) 772, 1412
XcmI CCANNNNNNNNNTGG 2 cut(s) 484, 1796
XmiI GTMKAC 1 cut(s) 539
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 5 cut(s) 492, 564, 1509, 1665, 2087
Zsp2I ATGCAT 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.