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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 58 |
| AciI | CCGC | 1 cut(s) | 259 |
| AclWI | GGATC | 1 cut(s) | 79 |
| AfaI | GTAC | 1 cut(s) | 267 |
| AgsI | TTSAA | 3 cut(s) | 278, 289, 334 |
| AluBI | AGCT | 2 cut(s) | 216, 245 |
| AluI | AGCT | 2 cut(s) | 216, 245 |
| AlwI | GGATC | 1 cut(s) | 79 |
| AoxI | GGCC | 1 cut(s) | 327 |
| AspLEI | GCGC | 2 cut(s) | 58, 60 |
| AspS9I | GGNCC | 1 cut(s) | 26 |
| AvaII | GGWCC | 1 cut(s) | 26 |
| BanII | GRGCYC | 1 cut(s) | 111 |
| BccI | CCATC | 2 cut(s) | 120, 345 |
| BfaI | CTAG | 1 cut(s) | 213 |
| Bme18I | GGWCC | 1 cut(s) | 26 |
| BmgT120I | GGNCC | 1 cut(s) | 26 |
| BmsI | GCATC | 1 cut(s) | 246 |
| BseMII | CTCAG | 1 cut(s) | 231 |
| BsePI | GCGCGC | 1 cut(s) | 56 |
| Bsh1236I | CGCG | 1 cut(s) | 58 |
| BshFI | GGCC | 1 cut(s) | 329 |
| BslFI | GGGAC | 2 cut(s) | 109, 149 |
| BsmFI | GGGAC | 2 cut(s) | 109, 149 |
| BsnI | GGCC | 1 cut(s) | 329 |
| Bsp1286I | GDGCHC | 1 cut(s) | 111 |
| Bsp1407I | TGTACA | 1 cut(s) | 265 |
| Bsp143I | GATC | 1 cut(s) | 71 |
| BspACI | CCGC | 1 cut(s) | 259 |
| BspANI | GGCC | 1 cut(s) | 329 |
| BspCNI | CTCAG | 1 cut(s) | 230 |
| BspFNI | CGCG | 1 cut(s) | 58 |
| BspPI | GGATC | 1 cut(s) | 79 |
| BsrGI | TGTACA | 1 cut(s) | 265 |
| BssHII | GCGCGC | 1 cut(s) | 56 |
| BssMI | GATC | 1 cut(s) | 71 |
| Bst4CI | ACNGT | 1 cut(s) | 88 |
| BstAUI | TGTACA | 1 cut(s) | 265 |
| BstC8I | GCNNGC | 2 cut(s) | 54, 58 |
| BstDEI | CTNAG | 2 cut(s) | 120, 217 |
| BstFNI | CGCG | 1 cut(s) | 58 |
| BstHHI | GCGC | 2 cut(s) | 58, 60 |
| BstKTI | GATC | 1 cut(s) | 74 |
| BstMBI | GATC | 1 cut(s) | 71 |
| BstUI | CGCG | 1 cut(s) | 58 |
| BsuRI | GGCC | 1 cut(s) | 329 |
| Cac8I | GCNNGC | 2 cut(s) | 54, 58 |
| CfoI | GCGC | 2 cut(s) | 58, 60 |
| Cfr13I | GGNCC | 1 cut(s) | 26 |
| Csp6I | GTAC | 1 cut(s) | 266 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 49, 84 |
| CviAII | CATG | 1 cut(s) | 337 |
| CviJI | RGCY | 4 cut(s) | 109, 216, 245, 329 |
| CviKI_1 | RGCY | 4 cut(s) | 109, 216, 245, 329 |
| CviQI | GTAC | 1 cut(s) | 266 |
| DdeI | CTNAG | 2 cut(s) | 120, 217 |
| DpnI | GATC | 1 cut(s) | 73 |
| DpnII | GATC | 1 cut(s) | 71 |
| Eco147I | AGGCCT | 1 cut(s) | 329 |
| Eco24I | GRGCYC | 1 cut(s) | 111 |
| Eco47I | GGWCC | 1 cut(s) | 26 |
| EcoT38I | GRGCYC | 1 cut(s) | 111 |
| FaeI | CATG | 1 cut(s) | 340 |
| FaiI | YATR | 3 cut(s) | 170, 318, 338 |
| FaqI | GGGAC | 2 cut(s) | 109, 149 |
| FatI | CATG | 1 cut(s) | 336 |
| FriOI | GRGCYC | 1 cut(s) | 111 |
| FspBI | CTAG | 1 cut(s) | 213 |
| GlaI | GCGC | 2 cut(s) | 57, 59 |
| HaeIII | GGCC | 1 cut(s) | 329 |
| HhaI | GCGC | 2 cut(s) | 58, 60 |
| Hin1II | CATG | 1 cut(s) | 340 |
| Hin6I | GCGC | 2 cut(s) | 56, 58 |
| HinP1I | GCGC | 2 cut(s) | 56, 58 |
| HincII | GTYRAC | 1 cut(s) | 84 |
| HindII | GTYRAC | 1 cut(s) | 84 |
| HinfI | GANTC | 1 cut(s) | 8 |
| Hpy166II | GTNNAC | 1 cut(s) | 84 |
| Hpy188I | TCNGA | 2 cut(s) | 220, 229 |
| Hpy8I | GTNNAC | 1 cut(s) | 84 |
| HpyAV | CCTTC | 2 cut(s) | 134, 275 |
| HpyCH4III | ACNGT | 1 cut(s) | 88 |
| HpyCH4V | TGCA | 1 cut(s) | 155 |
| HpyF3I | CTNAG | 2 cut(s) | 120, 217 |
| Hsp92II | CATG | 1 cut(s) | 340 |
| HspAI | GCGC | 2 cut(s) | 56, 58 |
| Kzo9I | GATC | 1 cut(s) | 71 |
| LmnI | GCTCC | 1 cut(s) | 214 |
| LpnPI | CCDG | 1 cut(s) | 38 |
| LweI | GCATC | 1 cut(s) | 246 |
| MaeI | CTAG | 1 cut(s) | 213 |
| MaeIII | GTNAC | 2 cut(s) | 44, 145 |
| MalI | GATC | 1 cut(s) | 73 |
| MboI | GATC | 1 cut(s) | 71 |
| MboII | GAAGA | 1 cut(s) | 290 |
| MhlI | GDGCHC | 1 cut(s) | 111 |
| MmeI | TCCRAC | 1 cut(s) | 70 |
| MnlI | CCTC | 2 cut(s) | 86, 319 |
| MseI | TTAA | 1 cut(s) | 302 |
| MvnI | CGCG | 1 cut(s) | 58 |
| NdeII | GATC | 1 cut(s) | 71 |
| NlaIII | CATG | 1 cut(s) | 340 |
| PauI | GCGCGC | 1 cut(s) | 56 |
| PceI | AGGCCT | 1 cut(s) | 329 |
| PfeI | GAWTC | 1 cut(s) | 8 |
| PspPI | GGNCC | 1 cut(s) | 26 |
| PteI | GCGCGC | 1 cut(s) | 56 |
| RsaI | GTAC | 1 cut(s) | 267 |
| RsaNI | GTAC | 1 cut(s) | 266 |
| SaqAI | TTAA | 1 cut(s) | 302 |
| Sau3AI | GATC | 1 cut(s) | 71 |
| Sau96I | GGNCC | 1 cut(s) | 26 |
| SduI | GDGCHC | 1 cut(s) | 111 |
| SetI | ASST | 2 cut(s) | 218, 247 |
| SfaNI | GCATC | 1 cut(s) | 246 |
| SinI | GGWCC | 1 cut(s) | 26 |
| SseBI | AGGCCT | 1 cut(s) | 329 |
| SsiI | CCGC | 1 cut(s) | 259 |
| SspMI | CTAG | 1 cut(s) | 213 |
| StuI | AGGCCT | 1 cut(s) | 329 |
| TaaI | ACNGT | 1 cut(s) | 88 |
| TaqII | GACCGA | 1 cut(s) | 14 |
| TatI | WGTACW | 1 cut(s) | 265 |
| TfiI | GAWTC | 1 cut(s) | 8 |
| Tru1I | TTAA | 1 cut(s) | 302 |
| Tru9I | TTAA | 1 cut(s) | 302 |
| TspDTI | ATGAA | 2 cut(s) | 17, 326 |
| VpaK11BI | GGWCC | 1 cut(s) | 26 |
| XspI | CTAG | 1 cut(s) | 213 |