transcription factor
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 874, 2950 |
| Acc16I | TGCGCA | 2 cut(s) | 126, 2202 |
| Acc36I | ACCTGC | 2 cut(s) | 874, 2950 |
| AccBSI | CCGCTC | 4 cut(s) | 35, 1615, 2111, 3691 |
| AccI | GTMKAC | 2 cut(s) | 519, 2595 |
| AccII | CGCG | 1 cut(s) | 1943 |
| AclWI | GGATC | 9 cut(s) | 336, 349, 572, 1630, 1795, 2412, 2425, 2648, 3706 |
| AcoI | YGGCCR | 2 cut(s) | 894, 2970 |
| AcsI | RAATTY | 4 cut(s) | 8, 1104, 2084, 3180 |
| AcuI | CTGAAG | 6 cut(s) | 603, 1275, 1431, 2679, 3351, 3507 |
| AfaI | GTAC | 3 cut(s) | 176, 2050, 2252 |
| AflII | CTTAAG | 1 cut(s) | 1733 |
| AhlI | ACTAGT | 4 cut(s) | 119, 440, 2195, 2516 |
| AjnI | CCWGG | 4 cut(s) | 213, 1050, 2289, 3126 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1054, 1086, 3130, 3162 |
| Alw21I | GWGCWC | 4 cut(s) | 629, 1759, 1778, 2705 |
| AlwI | GGATC | 9 cut(s) | 336, 349, 572, 1630, 1795, 2412, 2425, 2648, 3706 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1360, 3436 |
| Ama87I | CYCGRG | 2 cut(s) | 320, 2396 |
| ApoI | RAATTY | 4 cut(s) | 8, 1104, 2084, 3180 |
| AseI | ATTAAT | 2 cut(s) | 1344, 3420 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1440, 3516 |
| AspLEI | GCGC | 4 cut(s) | 127, 1368, 2203, 3444 |
| AsuC2I | CCSGG | 2 cut(s) | 1683, 3759 |
| AvaI | CYCGRG | 2 cut(s) | 320, 2396 |
| AvaII | GGWCC | 4 cut(s) | 1124, 1484, 3200, 3560 |
| AxyI | CCTNAGG | 4 cut(s) | 699, 875, 2775, 2951 |
| BalI | TGGCCA | 2 cut(s) | 896, 2972 |
| BamHI | GGATCC | 2 cut(s) | 341, 2417 |
| BanII | GRGCYC | 5 cut(s) | 1517, 1662, 1778, 3593, 3738 |
| BauI | CACGAG | 1 cut(s) | 1777 |
| BbsI | GAAGAC | 1 cut(s) | 1866 |
| Bbv12I | GWGCWC | 4 cut(s) | 629, 1759, 1778, 2705 |
| BceAI | ACGGC | 2 cut(s) | 854, 2930 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1682, 1716, 3758, 3792 |
| BciT130I | CCWGG | 4 cut(s) | 215, 1052, 2291, 3128 |
| BciVI | GTATCC | 2 cut(s) | 728, 2804 |
| BclI | TGATCA | 2 cut(s) | 1222, 3298 |
| BcnI | CCSGG | 2 cut(s) | 1683, 3759 |
| BcuI | ACTAGT | 4 cut(s) | 119, 440, 2195, 2516 |
| BfmI | CTRYAG | 7 cut(s) | 295, 1065, 1361, 1884, 2371, 3141, 3437 |
| BfoI | RGCGCY | 2 cut(s) | 1369, 3445 |
| BfrI | CTTAAG | 1 cut(s) | 1733 |
| BfuAI | ACCTGC | 2 cut(s) | 874, 2950 |
| BfuI | GTATCC | 2 cut(s) | 728, 2804 |
| BmcAI | AGTACT | 2 cut(s) | 176, 2252 |
| Bme1390I | CCNGG | 6 cut(s) | 215, 1052, 1683, 2291, 3128, 3759 |
| Bme18I | GGWCC | 4 cut(s) | 1124, 1484, 3200, 3560 |
| BmeT110I | CYCGRG | 2 cut(s) | 320, 2396 |
| BmiI | GGNNCC | 2 cut(s) | 343, 2419 |
| BmrFI | CCNGG | 6 cut(s) | 215, 1052, 1683, 2291, 3128, 3759 |
| BmsI | GCATC | 9 cut(s) | 270, 847, 1292, 1874, 1982, 2346, 2923, 3368, 3815 |
| BoxI | GACNNNNGTC | 2 cut(s) | 1587, 3663 |
| BpiI | GAAGAC | 1 cut(s) | 1866 |
| BpmI | CTGGAG | 2 cut(s) | 711, 2787 |
| BpuEI | CTTGAG | 4 cut(s) | 382, 539, 2458, 2615 |
| BpuMI | CCSGG | 2 cut(s) | 1683, 3759 |
| BsaI | GGTCTC | 3 cut(s) | 814, 1928, 2890 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 874, 904, 995, 1025, 2950, 2980, 3814, 3844 |
| Bse118I | RCCGGY | 2 cut(s) | 97, 2173 |
| Bse1I | ACTGG | 6 cut(s) | 415, 728, 1354, 2491, 2804, 3430 |
| Bse21I | CCTNAGG | 4 cut(s) | 699, 875, 2775, 2951 |
| Bse3DI | GCAATG | 1 cut(s) | 1860 |
| BseBI | CCWGG | 4 cut(s) | 215, 1052, 2291, 3128 |
| BseMI | GCAATG | 1 cut(s) | 1860 |
| BseNI | ACTGG | 6 cut(s) | 415, 728, 1354, 2491, 2804, 3430 |
| BseRI | GAGGAG | 5 cut(s) | 714, 1016, 1712, 1846, 2790 |
| BsgI | GTGCAG | 2 cut(s) | 1529, 3605 |
| Bsh1236I | CGCG | 1 cut(s) | 1943 |
| BsiHKAI | GWGCWC | 4 cut(s) | 629, 1759, 1778, 2705 |
| BsiHKCI | CYCGRG | 2 cut(s) | 320, 2396 |
| BsiSI | CCGG | 6 cut(s) | 98, 603, 1682, 2174, 2679, 3758 |
| BslFI | GGGAC | 5 cut(s) | 1660, 1700, 1767, 3736, 3776 |
| BsmFI | GGGAC | 5 cut(s) | 1660, 1700, 1767, 3736, 3776 |
| Bso31I | GGTCTC | 3 cut(s) | 814, 1928, 2890 |
| BsoBI | CYCGRG | 2 cut(s) | 320, 2396 |
| Bsp1286I | GDGCHC | 8 cut(s) | 629, 1517, 1662, 1759, 1778, 2705, 3593, 3738 |
| BspFNI | CGCG | 1 cut(s) | 1943 |
| BspHI | TCATGA | 2 cut(s) | 1894, 1978 |
| BspLI | GGNNCC | 2 cut(s) | 343, 2419 |
| BspMAI | CTGCAG | 4 cut(s) | 299, 1365, 2375, 3441 |
| BspMI | ACCTGC | 2 cut(s) | 874, 2950 |
| BspPI | GGATC | 9 cut(s) | 336, 349, 572, 1630, 1795, 2412, 2425, 2648, 3706 |
| BspTI | CTTAAG | 1 cut(s) | 1733 |
| BspTNI | GGTCTC | 3 cut(s) | 814, 1928, 2890 |
| BsrBI | CCGCTC | 4 cut(s) | 35, 1615, 2111, 3691 |
| BsrDI | GCAATG | 1 cut(s) | 1860 |
| BsrFI | RCCGGY | 2 cut(s) | 97, 2173 |
| BsrI | ACTGG | 6 cut(s) | 415, 728, 1354, 2491, 2804, 3430 |
| BssAI | RCCGGY | 2 cut(s) | 97, 2173 |
| BssNAI | GTATAC | 2 cut(s) | 520, 2596 |
| BssSI | CACGAG | 1 cut(s) | 1777 |
| BssT1I | CCWWGG | 4 cut(s) | 930, 1496, 3006, 3572 |
| Bst1107I | GTATAC | 2 cut(s) | 520, 2596 |
| Bst2BI | CACGAG | 1 cut(s) | 1777 |
| Bst2UI | CCWGG | 4 cut(s) | 215, 1052, 2291, 3128 |
| Bst6I | CTCTTC | 4 cut(s) | 791, 1730, 1755, 2867 |
| BstAFI | CTTAAG | 1 cut(s) | 1733 |
| BstC8I | GCNNGC | 6 cut(s) | 597, 1517, 1521, 2673, 3593, 3597 |
| BstDSI | CCRYGG | 1 cut(s) | 1941 |
| BstEII | GGTNACC | 2 cut(s) | 548, 2624 |
| BstFNI | CGCG | 1 cut(s) | 1943 |
| BstH2I | RGCGCY | 2 cut(s) | 1369, 3445 |
| BstHHI | GCGC | 4 cut(s) | 127, 1368, 2203, 3444 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 103, 866, 1516, 1537, 1940, 2179, 2942, 3592, 3613 |
| BstNI | CCWGG | 4 cut(s) | 215, 1052, 2291, 3128 |
| BstNSI | RCATGY | 4 cut(s) | 144, 1038, 2220, 3114 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 1587, 3663 |
| BstPI | GGTNACC | 2 cut(s) | 548, 2624 |
| BstSCI | CCNGG | 6 cut(s) | 213, 1050, 1681, 2289, 3126, 3757 |
| BstSFI | CTRYAG | 7 cut(s) | 295, 1065, 1361, 1884, 2371, 3141, 3437 |
| BstUI | CGCG | 1 cut(s) | 1943 |
| BstV2I | GAAGAC | 1 cut(s) | 1866 |
| BstX2I | RGATCY | 3 cut(s) | 341, 1800, 2417 |
| BstYI | RGATCY | 3 cut(s) | 341, 1800, 2417 |
| BstZ17I | GTATAC | 2 cut(s) | 520, 2596 |
| Bsu36I | CCTNAGG | 4 cut(s) | 699, 875, 2775, 2951 |
| BsuI | GTATCC | 2 cut(s) | 728, 2804 |
| BtgI | CCRYGG | 1 cut(s) | 1941 |
| BtgZI | GCGATG | 2 cut(s) | 1053, 3129 |
| BtsI | GCAGTG | 2 cut(s) | 1358, 3434 |
| BtsIMutI | CAGTG | 7 cut(s) | 471, 1204, 1358, 2547, 3280, 3434, 3857 |
| BveI | ACCTGC | 2 cut(s) | 874, 2950 |
| Cac8I | GCNNGC | 6 cut(s) | 597, 1517, 1521, 2673, 3593, 3597 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1360, 3436 |
| CciI | TCATGA | 2 cut(s) | 1894, 1978 |
| CfoI | GCGC | 4 cut(s) | 127, 1368, 2203, 3444 |
| Cfr10I | RCCGGY | 2 cut(s) | 97, 2173 |
| Cfr42I | CCGCGG | 1 cut(s) | 1944 |
| CseI | GACGC | 2 cut(s) | 416, 2492 |
| Csp6I | GTAC | 3 cut(s) | 175, 2049, 2251 |
| CviQI | GTAC | 3 cut(s) | 175, 2049, 2251 |
| EaeI | YGGCCR | 2 cut(s) | 894, 2970 |
| Eam1104I | CTCTTC | 4 cut(s) | 791, 1730, 1755, 2867 |
| EarI | CTCTTC | 4 cut(s) | 791, 1730, 1755, 2867 |
| EciI | GGCGGA | 4 cut(s) | 895, 1635, 2971, 3711 |
| Ecl136II | GAGCTC | 1 cut(s) | 1776 |
| Eco130I | CCWWGG | 4 cut(s) | 930, 1496, 3006, 3572 |
| Eco147I | AGGCCT | 1 cut(s) | 2059 |
| Eco24I | GRGCYC | 5 cut(s) | 1517, 1662, 1778, 3593, 3738 |
| Eco31I | GGTCTC | 3 cut(s) | 814, 1928, 2890 |
| Eco47I | GGWCC | 4 cut(s) | 1124, 1484, 3200, 3560 |
| Eco53kI | GAGCTC | 1 cut(s) | 1776 |
| Eco57I | CTGAAG | 6 cut(s) | 603, 1275, 1431, 2679, 3351, 3507 |
| Eco81I | CCTNAGG | 4 cut(s) | 699, 875, 2775, 2951 |
| Eco88I | CYCGRG | 2 cut(s) | 320, 2396 |
| Eco91I | GGTNACC | 2 cut(s) | 548, 2624 |
| EcoICRI | GAGCTC | 1 cut(s) | 1776 |
| EcoO109I | RGGNCCY | 3 cut(s) | 1303, 1836, 3379 |
| EcoO65I | GGTNACC | 2 cut(s) | 548, 2624 |
| EcoRII | CCWGG | 4 cut(s) | 213, 1050, 2289, 3126 |
| EcoT14I | CCWWGG | 4 cut(s) | 930, 1496, 3006, 3572 |
| EcoT38I | GRGCYC | 5 cut(s) | 1517, 1662, 1778, 3593, 3738 |
| ErhI | CCWWGG | 4 cut(s) | 930, 1496, 3006, 3572 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 728, 760, 2804, 2836 |
| FaqI | GGGAC | 5 cut(s) | 1660, 1700, 1767, 3736, 3776 |
| FauI | CCCGC | 6 cut(s) | 1435, 1608, 1737, 1936, 3511, 3684 |
| FauNDI | CATATG | 2 cut(s) | 1536, 3612 |
| FbaI | TGATCA | 2 cut(s) | 1222, 3298 |
| FblI | GTMKAC | 2 cut(s) | 519, 2595 |
| FriOI | GRGCYC | 5 cut(s) | 1517, 1662, 1778, 3593, 3738 |
| FspI | TGCGCA | 2 cut(s) | 126, 2202 |
| GlaI | GCGC | 4 cut(s) | 126, 1367, 2202, 3443 |
| GsuI | CTGGAG | 2 cut(s) | 711, 2787 |
| HaeII | RGCGCY | 2 cut(s) | 1369, 3445 |
| HapII | CCGG | 6 cut(s) | 98, 603, 1682, 2174, 2679, 3758 |
| HgaI | GACGC | 2 cut(s) | 416, 2492 |
| HhaI | GCGC | 4 cut(s) | 127, 1368, 2203, 3444 |
| Hin6I | GCGC | 4 cut(s) | 125, 1366, 2201, 3442 |
| HinP1I | GCGC | 4 cut(s) | 125, 1366, 2201, 3442 |
| HincII | GTYRAC | 2 cut(s) | 973, 3049 |
| HindII | GTYRAC | 2 cut(s) | 973, 3049 |
| HpaII | CCGG | 6 cut(s) | 98, 603, 1682, 2174, 2679, 3758 |
| Hpy99I | CGWCG | 4 cut(s) | 928, 1697, 3004, 3773 |
| HpyCH4IV | ACGT | 4 cut(s) | 916, 1695, 2992, 3771 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 103, 866, 1516, 1537, 1940, 2179, 2942, 3592, 3613 |
| HpySE526I | ACGT | 4 cut(s) | 916, 1695, 2992, 3771 |
| HspAI | GCGC | 4 cut(s) | 125, 1366, 2201, 3442 |
| Ksp22I | TGATCA | 2 cut(s) | 1222, 3298 |
| KspI | CCGCGG | 1 cut(s) | 1944 |
| LweI | GCATC | 9 cut(s) | 270, 847, 1292, 1874, 1982, 2346, 2923, 3368, 3815 |
| MaeII | ACGT | 4 cut(s) | 916, 1695, 2992, 3771 |
| MbiI | CCGCTC | 4 cut(s) | 35, 1615, 2111, 3691 |
| MflI | RGATCY | 3 cut(s) | 341, 1800, 2417 |
| MhlI | GDGCHC | 8 cut(s) | 629, 1517, 1662, 1759, 1778, 2705, 3593, 3738 |
| MlsI | TGGCCA | 2 cut(s) | 896, 2972 |
| MluNI | TGGCCA | 2 cut(s) | 896, 2972 |
| MlyI | GAGTC | 4 cut(s) | 1019, 1741, 1880, 3095 |
| MmeI | TCCRAC | 7 cut(s) | 319, 1085, 1621, 1724, 2395, 3161, 3697 |
| Mox20I | TGGCCA | 2 cut(s) | 896, 2972 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1440, 3516 |
| MscI | TGGCCA | 2 cut(s) | 896, 2972 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1436, 3512 |
| Msp20I | TGGCCA | 2 cut(s) | 896, 2972 |
| MspA1I | CMGCKG | 5 cut(s) | 382, 599, 1943, 2458, 2675 |
| MspCI | CTTAAG | 1 cut(s) | 1733 |
| MspI | CCGG | 6 cut(s) | 98, 603, 1682, 2174, 2679, 3758 |
| MspR9I | CCNGG | 6 cut(s) | 215, 1052, 1683, 2291, 3128, 3759 |
| MvaI | CCWGG | 4 cut(s) | 215, 1052, 2291, 3128 |
| MvnI | CGCG | 1 cut(s) | 1943 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 103, 866, 1516, 1537, 1940, 2179, 2942, 3592, 3613 |
| NciI | CCSGG | 2 cut(s) | 1683, 3759 |
| NdeI | CATATG | 2 cut(s) | 1536, 3612 |
| NlaIV | GGNNCC | 2 cut(s) | 343, 2419 |
| NmuCI | GTSAC | 4 cut(s) | 548, 1356, 2624, 3432 |
| NsbI | TGCGCA | 2 cut(s) | 126, 2202 |
| NspI | RCATGY | 4 cut(s) | 144, 1038, 2220, 3114 |
| PagI | TCATGA | 2 cut(s) | 1894, 1978 |
| PaqCI | CACCTGC | 2 cut(s) | 874, 2950 |
| PceI | AGGCCT | 1 cut(s) | 2059 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1440, 3516 |
| PfeI | GAWTC | 7 cut(s) | 1423, 1571, 1792, 1808, 1826, 3499, 3647 |
| PleI | GAGTC | 4 cut(s) | 1018, 1741, 1879, 3094 |
| PpsI | GAGTC | 4 cut(s) | 1018, 1741, 1879, 3094 |
| PshAI | GACNNNNGTC | 2 cut(s) | 1587, 3663 |
| PshBI | ATTAAT | 2 cut(s) | 1344, 3420 |
| Psp124BI | GAGCTC | 1 cut(s) | 1778 |
| Psp6I | CCWGG | 4 cut(s) | 213, 1050, 2289, 3126 |
| PspEI | GGTNACC | 2 cut(s) | 548, 2624 |
| PspGI | CCWGG | 4 cut(s) | 213, 1050, 2289, 3126 |
| PspN4I | GGNNCC | 2 cut(s) | 343, 2419 |
| PstI | CTGCAG | 4 cut(s) | 299, 1365, 2375, 3441 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1360, 3436 |
| PsuI | RGATCY | 3 cut(s) | 341, 1800, 2417 |
| PvuII | CAGCTG | 2 cut(s) | 599, 2675 |
| RsaI | GTAC | 3 cut(s) | 176, 2050, 2252 |
| RsaNI | GTAC | 3 cut(s) | 175, 2049, 2251 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1436, 3512 |
| SacI | GAGCTC | 1 cut(s) | 1778 |
| SacII | CCGCGG | 1 cut(s) | 1944 |
| SbfI | CCTGCAGG | 2 cut(s) | 299, 2375 |
| ScaI | AGTACT | 2 cut(s) | 176, 2252 |
| SchI | GAGTC | 4 cut(s) | 1019, 1741, 1880, 3095 |
| ScrFI | CCNGG | 6 cut(s) | 215, 1052, 1683, 2291, 3128, 3759 |
| SdaI | CCTGCAGG | 2 cut(s) | 299, 2375 |
| SduI | GDGCHC | 8 cut(s) | 629, 1517, 1662, 1759, 1778, 2705, 3593, 3738 |
| SfaNI | GCATC | 9 cut(s) | 270, 847, 1292, 1874, 1982, 2346, 2923, 3368, 3815 |
| SfcI | CTRYAG | 7 cut(s) | 295, 1065, 1361, 1884, 2371, 3141, 3437 |
| Sfr303I | CCGCGG | 1 cut(s) | 1944 |
| SgrBI | CCGCGG | 1 cut(s) | 1944 |
| SinI | GGWCC | 4 cut(s) | 1124, 1484, 3200, 3560 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1436, 3512 |
| SmlI | CTYRAG | 5 cut(s) | 361, 554, 1733, 2437, 2630 |
| SmoI | CTYRAG | 5 cut(s) | 361, 554, 1733, 2437, 2630 |
| SpeI | ACTAGT | 4 cut(s) | 119, 440, 2195, 2516 |
| Sse8387I | CCTGCAGG | 2 cut(s) | 299, 2375 |
| SseBI | AGGCCT | 1 cut(s) | 2059 |
| SstI | GAGCTC | 1 cut(s) | 1778 |
| StuI | AGGCCT | 1 cut(s) | 2059 |
| StyD4I | CCNGG | 6 cut(s) | 213, 1050, 1681, 2289, 3126, 3757 |
| StyI | CCWWGG | 4 cut(s) | 930, 1496, 3006, 3572 |
| TaiI | ACGT | 4 cut(s) | 919, 1698, 2995, 3774 |
| TatI | WGTACW | 2 cut(s) | 174, 2250 |
| TauI | GCSGC | 2 cut(s) | 35, 2111 |
| TfiI | GAWTC | 7 cut(s) | 1423, 1571, 1792, 1808, 1826, 3499, 3647 |
| TscAI | CASTG | 7 cut(s) | 478, 1204, 1365, 2554, 3280, 3441, 3864 |
| TseFI | GTSAC | 4 cut(s) | 548, 1356, 2624, 3432 |
| Tsp45I | GTSAC | 4 cut(s) | 548, 1356, 2624, 3432 |
| TspGWI | ACGGA | 2 cut(s) | 1137, 3213 |
| TspRI | CASTG | 7 cut(s) | 478, 1204, 1365, 2554, 3280, 3441, 3864 |
| Vha464I | CTTAAG | 1 cut(s) | 1733 |
| VpaK11BI | GGWCC | 4 cut(s) | 1124, 1484, 3200, 3560 |
| VspI | ATTAAT | 2 cut(s) | 1344, 3420 |
| XapI | RAATTY | 4 cut(s) | 8, 1104, 2084, 3180 |
| XbaI | TCTAGA | 2 cut(s) | 491, 2567 |
| XceI | RCATGY | 4 cut(s) | 144, 1038, 2220, 3114 |
| XmiI | GTMKAC | 2 cut(s) | 519, 2595 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1440, 3516 |
| ZrmI | AGTACT | 2 cut(s) | 176, 2252 |