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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 54 |
| AfaI | GTAC | 1 cut(s) | 87 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 328 |
| AflIII | ACRYGT | 1 cut(s) | 578 |
| AgsI | TTSAA | 1 cut(s) | 136 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 168, 200 |
| AluBI | AGCT | 1 cut(s) | 184 |
| AluI | AGCT | 1 cut(s) | 184 |
| Alw26I | GTCTC | 2 cut(s) | 267, 587 |
| ApeKI | GCWGC | 1 cut(s) | 181 |
| AsuHPI | GGTGA | 2 cut(s) | 220, 382 |
| BbvI | GCAGC | 1 cut(s) | 193 |
| BccI | CCATC | 2 cut(s) | 106, 183 |
| BclI | TGATCA | 2 cut(s) | 162, 256 |
| BcoDI | GTCTC | 2 cut(s) | 267, 587 |
| BfaI | CTAG | 1 cut(s) | 293 |
| BfmI | CTRYAG | 2 cut(s) | 179, 309 |
| BisI | GCNGC | 1 cut(s) | 182 |
| BlsI | GCNGC | 1 cut(s) | 183 |
| BmsI | GCATC | 1 cut(s) | 6 |
| BpmI | CTGGAG | 1 cut(s) | 92 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 328 |
| Bse1I | ACTGG | 1 cut(s) | 283 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 328 |
| BseNI | ACTGG | 1 cut(s) | 283 |
| BseXI | GCAGC | 1 cut(s) | 193 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 328 |
| BsmAI | GTCTC | 2 cut(s) | 267, 587 |
| Bsp143I | GATC | 3 cut(s) | 6, 162, 256 |
| BspHI | TCATGA | 1 cut(s) | 253 |
| BspMAI | CTGCAG | 1 cut(s) | 183 |
| BsrI | ACTGG | 1 cut(s) | 283 |
| BssMI | GATC | 3 cut(s) | 6, 162, 256 |
| Bst4CI | ACNGT | 1 cut(s) | 205 |
| Bst6I | CTCTTC | 1 cut(s) | 188 |
| BstC8I | GCNNGC | 1 cut(s) | 423 |
| BstDEI | CTNAG | 1 cut(s) | 10 |
| BstEII | GGTNACC | 1 cut(s) | 169 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 326 |
| BstKTI | GATC | 3 cut(s) | 9, 165, 259 |
| BstMAI | GTCTC | 2 cut(s) | 267, 587 |
| BstMBI | GATC | 3 cut(s) | 6, 162, 256 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 39, 552 |
| BstNSI | RCATGY | 2 cut(s) | 429, 582 |
| BstPI | GGTNACC | 1 cut(s) | 169 |
| BstSFI | CTRYAG | 2 cut(s) | 179, 309 |
| BstV1I | GCAGC | 1 cut(s) | 193 |
| BtsIMutI | CAGTG | 1 cut(s) | 276 |
| Cac8I | GCNNGC | 1 cut(s) | 423 |
| CciI | TCATGA | 1 cut(s) | 253 |
| Csp6I | GTAC | 1 cut(s) | 86 |
| CviAII | CATG | 6 cut(s) | 254, 426, 487, 540, 567, 579 |
| CviJI | RGCY | 6 cut(s) | 42, 184, 215, 321, 482, 499 |
| CviKI_1 | RGCY | 6 cut(s) | 42, 184, 215, 321, 482, 499 |
| CviQI | GTAC | 1 cut(s) | 86 |
| DdeI | CTNAG | 1 cut(s) | 10 |
| DpnI | GATC | 3 cut(s) | 8, 164, 258 |
| DpnII | GATC | 3 cut(s) | 6, 162, 256 |
| Eam1104I | CTCTTC | 1 cut(s) | 188 |
| EarI | CTCTTC | 1 cut(s) | 188 |
| Eco91I | GGTNACC | 1 cut(s) | 169 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 326 |
| EcoO65I | GGTNACC | 1 cut(s) | 169 |
| FaeI | CATG | 6 cut(s) | 257, 429, 490, 543, 570, 582 |
| FatI | CATG | 6 cut(s) | 253, 425, 486, 539, 566, 578 |
| FbaI | TGATCA | 2 cut(s) | 162, 256 |
| Fnu4HI | GCNGC | 1 cut(s) | 182 |
| Fsp4HI | GCNGC | 1 cut(s) | 182 |
| FspBI | CTAG | 1 cut(s) | 293 |
| GluI | GCNGC | 1 cut(s) | 182 |
| GsuI | CTGGAG | 1 cut(s) | 92 |
| Hin1II | CATG | 6 cut(s) | 257, 429, 490, 543, 570, 582 |
| HinfI | GANTC | 6 cut(s) | 80, 127, 250, 302, 377, 548 |
| HphI | GGTGA | 2 cut(s) | 220, 382 |
| Hpy188III | TCNNGA | 2 cut(s) | 71, 254 |
| HpyAV | CCTTC | 1 cut(s) | 292 |
| HpyCH4III | ACNGT | 1 cut(s) | 205 |
| HpyCH4V | TGCA | 4 cut(s) | 19, 181, 450, 555 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 39, 552 |
| HpyF3I | CTNAG | 1 cut(s) | 10 |
| Hsp92II | CATG | 6 cut(s) | 257, 429, 490, 543, 570, 582 |
| Ksp22I | TGATCA | 2 cut(s) | 162, 256 |
| Kzo9I | GATC | 3 cut(s) | 6, 162, 256 |
| LpnPI | CCDG | 4 cut(s) | 56, 151, 264, 407 |
| Lsp1109I | GCAGC | 1 cut(s) | 193 |
| LweI | GCATC | 1 cut(s) | 6 |
| MaeI | CTAG | 1 cut(s) | 293 |
| MaeIII | GTNAC | 2 cut(s) | 169, 338 |
| MalI | GATC | 3 cut(s) | 8, 164, 258 |
| MboI | GATC | 3 cut(s) | 6, 162, 256 |
| MboII | GAAGA | 3 cut(s) | 148, 205, 518 |
| MluCI | AATT | 1 cut(s) | 439 |
| MlyI | GAGTC | 1 cut(s) | 557 |
| MnlI | CCTC | 6 cut(s) | 28, 87, 135, 189, 322, 397 |
| MslI | CAYNNNNRTG | 1 cut(s) | 491 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 39, 552 |
| NdeII | GATC | 3 cut(s) | 6, 162, 256 |
| NlaIII | CATG | 6 cut(s) | 257, 429, 490, 543, 570, 582 |
| NspI | RCATGY | 2 cut(s) | 429, 582 |
| PagI | TCATGA | 1 cut(s) | 253 |
| PciI | ACATGT | 1 cut(s) | 578 |
| PfeI | GAWTC | 5 cut(s) | 80, 127, 250, 302, 377 |
| PkrI | GCNGC | 1 cut(s) | 183 |
| PleI | GAGTC | 1 cut(s) | 556 |
| PpsI | GAGTC | 1 cut(s) | 556 |
| PscI | ACATGT | 1 cut(s) | 578 |
| PsiI | TTATAA | 1 cut(s) | 54 |
| PspEI | GGTNACC | 1 cut(s) | 169 |
| PstI | CTGCAG | 1 cut(s) | 183 |
| RsaI | GTAC | 1 cut(s) | 87 |
| RsaNI | GTAC | 1 cut(s) | 86 |
| RseI | CAYNNNNRTG | 1 cut(s) | 491 |
| SatI | GCNGC | 1 cut(s) | 182 |
| Sau3AI | GATC | 3 cut(s) | 6, 162, 256 |
| SchI | GAGTC | 1 cut(s) | 557 |
| SetI | ASST | 2 cut(s) | 106, 186 |
| SfaNI | GCATC | 1 cut(s) | 6 |
| SfcI | CTRYAG | 2 cut(s) | 179, 309 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 491 |
| Sse9I | AATT | 1 cut(s) | 439 |
| SspMI | CTAG | 1 cut(s) | 293 |
| TaaI | ACNGT | 1 cut(s) | 205 |
| TaqI | TCGA | 1 cut(s) | 187 |
| TasI | AATT | 1 cut(s) | 439 |
| TatI | WGTACW | 1 cut(s) | 85 |
| TfiI | GAWTC | 5 cut(s) | 80, 127, 250, 302, 377 |
| TscAI | CASTG | 1 cut(s) | 283 |
| TseI | GCWGC | 1 cut(s) | 181 |
| TspDTI | ATGAA | 3 cut(s) | 218, 528, 555 |
| TspRI | CASTG | 1 cut(s) | 283 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 326 |
| XceI | RCATGY | 2 cut(s) | 429, 582 |
| XspI | CTAG | 1 cut(s) | 293 |