Rroxscaffold_7G00196800

membrane protein At4g09580-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008356
Physical Location & Seq
Forward (+)
40045000 .. 40049481
4482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00196800.1

Sequence Viewer

Length: 843 bp
ATGATGGCGGCTCCGAGAAATCTGGTGGGGCTCAGGGATGAAGAGAATGCCAAGGCGGCGGCCGAGGAGGACCAGCAGGACGATGATTGGTCCCCCGGCAAGAAGCCCAAGTCGGAGCGCTTGCCCCTCACCCGCTGGGAGGTCGCCGCTGCTCTCTCCGTCTTCTTCATATTCTCCTCCGGCCTTTTCTGTGTCTACTTGACCATGCCCGCCGCCGATTACGGTAAACTCAAGCTTCCACGCACCATTGCCGATCTTCGCTTGCTCAAAGATCATCTTGGGACCTATGCAAACGATTATCCAGCTCAATTCATTCTGGGTTACTGTTCCACATACATCTTCATGCAGACTTTCATGATTCCCGGCACGATATTCATGTCATTGCTGGCCGGAGCTCTTTTCGGGGTTTTCAGGGGCCTTCTCTTGGTTGTCTTCAACGCCACAGCTGGAGCTTCTTCCTGCTTCTTTTTGTCCAAGTTAATTGGCAGGCCTTTAGTTAGTTGGTTGTGGCCCGAAAAATTGAGGTTCTTTCAGGCAGAGATTGCAAAGCGCAGGGAAAAGCTGCTCAACTATATGCTTTTCCTTAGGATAACTCCAACATTGCCAAACCTTTTCATCAACTTGGCATCTCCGATTGTTGACATACCCTTTCACATTTTCTTTCTTGCAACTTTGATCGGTCTTATTCCAGCATCTTATATCACAGTCAGAGCTGGCCTTGCTCTTGGGGATCTCAAGTCAGTCAAGGACCTCTACGATTTTAAAACATTATTTGTGCTTTTCCTTATTGGTTCAGTGATTATATTTCCAACACTTTTGAAGAGAAAGCGAATCTATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

280

Amino Acids

31.62

Weight (kDa)

9.36

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 120 - 239 1.7e-15 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 195
AciI CCGC 7 cut(s) 8, 56, 59, 133, 147, 210, 213
AclWI GGATC 1 cut(s) 738
AcoI YGGCCR 2 cut(s) 60, 387
AfeI AGCGCT 1 cut(s) 119
AfiI CCNNNNNNNGG 2 cut(s) 139, 424
AgsI TTSAA 2 cut(s) 436, 820
AluBI AGCT 7 cut(s) 235, 305, 395, 446, 452, 562, 713
AluI AGCT 7 cut(s) 235, 305, 395, 446, 452, 562, 713
Alw21I GWGCWC 1 cut(s) 397
AlwI GGATC 1 cut(s) 738
Aor51HI AGCGCT 1 cut(s) 119
AoxI GGCC 7 cut(s) 60, 181, 387, 415, 488, 509, 715
ApeKI GCWGC 2 cut(s) 149, 562
AspLEI GCGC 2 cut(s) 120, 552
AspS9I GGNCC 6 cut(s) 70, 90, 282, 415, 510, 748
AsuC2I CCSGG 2 cut(s) 96, 363
AsuHPI GGTGA 1 cut(s) 121
AvaII GGWCC 4 cut(s) 70, 90, 282, 748
AxyI CCTNAGG 1 cut(s) 584
BanII GRGCYC 2 cut(s) 33, 397
BbsI GAAGAC 2 cut(s) 154, 424
Bbv12I GWGCWC 1 cut(s) 397
BbvI GCAGC 2 cut(s) 136, 549
BcnI CCSGG 2 cut(s) 96, 363
BfoI RGCGCY 1 cut(s) 121
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 7 cut(s) 9, 57, 60, 147, 150, 213, 563
BlsI GCNGC 7 cut(s) 10, 58, 61, 148, 151, 214, 564
Bme1390I CCNGG 2 cut(s) 96, 363
Bme18I GGWCC 4 cut(s) 70, 90, 282, 748
BmgT120I GGNCC 6 cut(s) 70, 90, 282, 415, 510, 748
BmiI GGNNCC 4 cut(s) 12, 92, 283, 416
BmrFI CCNGG 2 cut(s) 96, 363
BmsI GCATC 2 cut(s) 635, 701
BpiI GAAGAC 2 cut(s) 154, 424
BpmI CTGGAG 1 cut(s) 468
Bpu10I CCTNAGC 1 cut(s) 32
BpuEI CTTGAG 2 cut(s) 215, 719
BpuMI CCSGG 2 cut(s) 96, 363
BsaJI CCNNGG 3 cut(s) 51, 63, 94
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 424
Bse21I CCTNAGG 1 cut(s) 584
Bse3DI GCAATG 3 cut(s) 246, 380, 599
BseDI CCNNGG 3 cut(s) 51, 63, 94
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 2 cut(s) 139, 424
BseMI GCAATG 3 cut(s) 246, 380, 599
BseMII CTCAG 1 cut(s) 46
BseRI GAGGAG 2 cut(s) 80, 166
BseX3I CGGCCG 1 cut(s) 60
BseXI GCAGC 2 cut(s) 136, 549
BseYI CCCAGC 1 cut(s) 135
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 7 cut(s) 62, 183, 389, 417, 490, 511, 717
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 397
BsiSI CCGG 4 cut(s) 96, 180, 363, 390
BslFI GGGAC 2 cut(s) 76, 295
BslI CCNNNNNNNGG 2 cut(s) 139, 424
BsmFI GGGAC 2 cut(s) 76, 295
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 7 cut(s) 62, 183, 389, 417, 490, 511, 717
Bsp1286I GDGCHC 2 cut(s) 33, 397
Bsp143I GATC 4 cut(s) 253, 271, 675, 730
BspACI CCGC 7 cut(s) 8, 56, 59, 133, 147, 210, 213
BspANI GGCC 7 cut(s) 62, 183, 389, 417, 490, 511, 717
BspCNI CTCAG 1 cut(s) 45
BspHI TCATGA 1 cut(s) 354
BspLI GGNNCC 4 cut(s) 12, 92, 283, 416
BspPI GGATC 1 cut(s) 738
BsrDI GCAATG 3 cut(s) 246, 380, 599
BssECI CCNNGG 3 cut(s) 51, 63, 94
BssMI GATC 4 cut(s) 253, 271, 675, 730
BssT1I CCWWGG 1 cut(s) 51
Bst4CI ACNGT 3 cut(s) 224, 326, 706
Bst6I CTCTTC 2 cut(s) 36, 815
BstAPI GCANNNNNTGC 1 cut(s) 542
BstC8I GCNNGC 6 cut(s) 122, 210, 263, 387, 488, 715
BstDEI CTNAG 2 cut(s) 32, 584
BstF5I GGATG 1 cut(s) 43
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 2 cut(s) 120, 552
BstKTI GATC 4 cut(s) 256, 274, 678, 733
BstMBI GATC 4 cut(s) 253, 271, 675, 730
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 3 cut(s) 56, 542, 719
BstSCI CCNGG 2 cut(s) 94, 361
BstV1I GCAGC 2 cut(s) 136, 549
BstV2I GAAGAC 2 cut(s) 154, 424
BstX2I RGATCY 1 cut(s) 730
BstYI RGATCY 1 cut(s) 730
BstZI CGGCCG 1 cut(s) 60
Bsu36I CCTNAGG 1 cut(s) 584
BsuRI GGCC 7 cut(s) 62, 183, 389, 417, 490, 511, 717
BtsCI GGATG 1 cut(s) 43
BtsIMutI CAGTG 1 cut(s) 801
Cac8I GCNNGC 6 cut(s) 122, 210, 263, 387, 488, 715
CciI TCATGA 1 cut(s) 354
CfoI GCGC 2 cut(s) 120, 552
Cfr13I GGNCC 6 cut(s) 70, 90, 282, 415, 510, 748
CviAII CATG 4 cut(s) 205, 343, 355, 376
DdeI CTNAG 2 cut(s) 32, 584
DpnI GATC 4 cut(s) 255, 273, 677, 732
DpnII GATC 4 cut(s) 253, 271, 675, 730
DraI TTTAAA 1 cut(s) 763
EaeI YGGCCR 2 cut(s) 60, 387
EagI CGGCCG 1 cut(s) 60
Eam1104I CTCTTC 2 cut(s) 36, 815
EarI CTCTTC 2 cut(s) 36, 815
Ecl136II GAGCTC 1 cut(s) 395
EclXI CGGCCG 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 51
Eco147I AGGCCT 1 cut(s) 490
Eco24I GRGCYC 2 cut(s) 33, 397
Eco47I GGWCC 4 cut(s) 70, 90, 282, 748
Eco47III AGCGCT 1 cut(s) 119
Eco52I CGGCCG 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 395
Eco81I CCTNAGG 1 cut(s) 584
EcoICRI GAGCTC 1 cut(s) 395
EcoO109I RGGNCCY 3 cut(s) 282, 415, 748
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 33, 397
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 4 cut(s) 208, 346, 358, 379
FalI AAGNNNNNCTT 2 cut(s) 261, 293
FaqI GGGAC 2 cut(s) 76, 295
FatI CATG 4 cut(s) 204, 342, 354, 375
FauI CCCGC 2 cut(s) 140, 217
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 7 cut(s) 9, 57, 60, 147, 150, 213, 563
FokI GGATG 1 cut(s) 50
FriOI GRGCYC 2 cut(s) 33, 397
Fsp4HI GCNGC 7 cut(s) 9, 57, 60, 147, 150, 213, 563
GlaI GCGC 2 cut(s) 119, 551
GluI GCNGC 7 cut(s) 9, 57, 60, 147, 150, 213, 563
GsaI CCCAGC 1 cut(s) 139
GsuI CTGGAG 1 cut(s) 468
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 7 cut(s) 62, 183, 389, 417, 490, 511, 717
HapII CCGG 4 cut(s) 96, 180, 363, 390
HhaI GCGC 2 cut(s) 120, 552
Hin1II CATG 4 cut(s) 208, 346, 358, 379
Hin6I GCGC 2 cut(s) 118, 550
HinP1I GCGC 2 cut(s) 118, 550
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 2 cut(s) 358, 831
HpaII CCGG 4 cut(s) 96, 180, 363, 390
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 3 cut(s) 196, 227, 640
Hpy188I TCNGA 4 cut(s) 15, 115, 633, 710
Hpy188III TCNNGA 1 cut(s) 355
Hpy8I GTNNAC 3 cut(s) 196, 227, 640
HpyAV CCTTC 1 cut(s) 428
HpyCH4III ACNGT 3 cut(s) 224, 326, 706
HpyCH4V TGCA 4 cut(s) 290, 346, 545, 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 542, 719
HpyF3I CTNAG 2 cut(s) 32, 584
Hsp92II CATG 4 cut(s) 208, 346, 358, 379
HspAI GCGC 2 cut(s) 118, 550
Kzo9I GATC 4 cut(s) 253, 271, 675, 730
LmnI GCTCC 4 cut(s) 16, 115, 392, 449
Lsp1109I GCAGC 2 cut(s) 136, 549
LweI GCATC 2 cut(s) 635, 701
MaeIII GTNAC 1 cut(s) 320
MalI GATC 4 cut(s) 255, 273, 677, 732
MboI GATC 4 cut(s) 253, 271, 675, 730
MboII GAAGA 8 cut(s) 53, 154, 157, 248, 331, 424, 447, 832
MflI RGATCY 1 cut(s) 730
MhlI GDGCHC 2 cut(s) 33, 397
MluCI AATT 3 cut(s) 308, 480, 518
MmeI TCCRAC 3 cut(s) 93, 620, 833
MnlI CCTC 7 cut(s) 58, 61, 133, 137, 187, 516, 761
MseI TTAA 2 cut(s) 479, 762
MslI CAYNNNNRTG 1 cut(s) 341
MspA1I CMGCKG 3 cut(s) 135, 149, 446
MspI CCGG 4 cut(s) 96, 180, 363, 390
MspR9I CCNGG 2 cut(s) 96, 363
Mva1269I GAATGC 1 cut(s) 52
MwoI GCNNNNNNNGC 3 cut(s) 56, 542, 719
NciI CCSGG 2 cut(s) 96, 363
NdeII GATC 4 cut(s) 253, 271, 675, 730
NlaIII CATG 4 cut(s) 208, 346, 358, 379
NlaIV GGNNCC 4 cut(s) 12, 92, 283, 416
NmeAIII GCCGAG 1 cut(s) 88
PagI TCATGA 1 cut(s) 354
PceI AGGCCT 1 cut(s) 490
PctI GAATGC 1 cut(s) 52
PfeI GAWTC 2 cut(s) 358, 831
PkrI GCNGC 7 cut(s) 10, 58, 61, 148, 151, 214, 564
PpuMI RGGWCCY 2 cut(s) 282, 748
Psp124BI GAGCTC 1 cut(s) 397
Psp5II RGGWCCY 2 cut(s) 282, 748
PspFI CCCAGC 1 cut(s) 135
PspN4I GGNNCC 4 cut(s) 12, 92, 283, 416
PspPI GGNCC 6 cut(s) 70, 90, 282, 415, 510, 748
PspPPI RGGWCCY 2 cut(s) 282, 748
PsuI RGATCY 1 cut(s) 730
PvuII CAGCTG 1 cut(s) 446
RseI CAYNNNNRTG 1 cut(s) 341
SacI GAGCTC 1 cut(s) 397
SaqAI TTAA 2 cut(s) 479, 762
SatI GCNGC 7 cut(s) 9, 57, 60, 147, 150, 213, 563
Sau3AI GATC 4 cut(s) 253, 271, 675, 730
Sau96I GGNCC 6 cut(s) 70, 90, 282, 415, 510, 748
ScrFI CCNGG 2 cut(s) 96, 363
SduI GDGCHC 2 cut(s) 33, 397
SfaNI GCATC 2 cut(s) 635, 701
SinI GGWCC 4 cut(s) 70, 90, 282, 748
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 2 cut(s) 230, 734
SmoI CTYRAG 2 cut(s) 230, 734
Sse9I AATT 3 cut(s) 308, 480, 518
SseBI AGGCCT 1 cut(s) 490
SsiI CCGC 7 cut(s) 8, 56, 59, 133, 147, 210, 213
SstI GAGCTC 1 cut(s) 397
StuI AGGCCT 1 cut(s) 490
StyD4I CCNGG 2 cut(s) 94, 361
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 3 cut(s) 224, 326, 706
TaqII GACCGA 1 cut(s) 668
TasI AATT 3 cut(s) 308, 480, 518
TauI GCSGC 5 cut(s) 11, 59, 62, 149, 215
TfiI GAWTC 2 cut(s) 358, 831
Tru1I TTAA 2 cut(s) 479, 762
Tru9I TTAA 2 cut(s) 479, 762
TscAI CASTG 1 cut(s) 801
TseI GCWGC 2 cut(s) 149, 562
TspDTI ATGAA 7 cut(s) 54, 157, 301, 331, 343, 364, 604
TspGWI ACGGA 1 cut(s) 148
TspRI CASTG 1 cut(s) 801
VpaK11BI GGWCC 4 cut(s) 70, 90, 282, 748
XmiI GTMKAC 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.