RLG00000013710

membrane protein At4g09580-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008356
Physical Location & Seq
Reverse (-)
36270801 .. 36275128
4328 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013710

Sequence Viewer

Length: 846 bp
ATGATGGCGGCTCCGAGAAATCTGGTGGGGCTCAGGGATGAAGAGAATGCCAAGGCGGCGGCGGCCGAGGAGGAGCAGCAGGACGATGATTGGTCCACCGGCAAGAAGCCCAAGTCGGAGCGCTTGCCCCTCACGCGCTGGGAGGTCGCCGCTGCTCTCTCCGTCTTCTTCATTTTCTCCTCCGGCCTTTTCTGTGTCTATTTGACCATGCCCGCCGCTGATTACGGTAAACTCAAGCTTCCACGCACCATTGCCGATCTTCGCTTGCTCAAAGATCATCTTGGGACCTATGCAACCGATTATCCAGCTCAATTCATTCTGGGTTACTGTTCCACATACATCTTCATGCAGACTTTCATGATTCCGGGAACGATATTCATGTCATTGCTGGCCGGAGCTCTTTTCGGGGTTTTCAGGGGCCTTCTCTTGGTTGTCTTCAACGCCACAGCTGGAGCTTCTTCCTGCTTCTTTTTGTCCAAGTTAATTGGCAGGCCTTTAGTTAGTTGGTTGTGGCCCGAAAAATTGAGATTCTTTCAGGCAGAGATTGCAAAGCGCAGGGAAAAGCTGCTCAACTATATGCTTTTCCTGAGGATAACTCCAACTTTGCCAAACCTTTTCATCAACTTGGCATCTCCCATTGTTGACATACCCTTTCACATTTTCTTTCTTGCAACTTTGATTGGCCTTATTCCAGCATCTTATATCACAGTCAGAGCTGGCCTTGCTCTTGGGGATCTCAAGTCAGTGAAGGACCTCTACGATTTTAAAACATTATTTGTGCTTTTCCTTATTGGTTCAGTGATTATATTTCCAACACTTTTGAAGAGAAAGCGAATCTATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

282

Amino Acids

31.69

Weight (kDa)

9.36

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 121 - 240 1.7e-15 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 136
AciI CCGC 7 cut(s) 8, 56, 59, 62, 150, 213, 216
AclWI GGATC 1 cut(s) 741
AcoI YGGCCR 2 cut(s) 63, 390
AfeI AGCGCT 1 cut(s) 122
AfiI CCNNNNNNNGG 1 cut(s) 427
AgsI TTSAA 2 cut(s) 439, 823
AluBI AGCT 7 cut(s) 238, 308, 398, 449, 455, 565, 716
AluI AGCT 7 cut(s) 238, 308, 398, 449, 455, 565, 716
Alw21I GWGCWC 1 cut(s) 400
AlwI GGATC 1 cut(s) 741
Aor51HI AGCGCT 1 cut(s) 122
AoxI GGCC 8 cut(s) 63, 184, 390, 418, 491, 512, 682, 718
ApeKI GCWGC 3 cut(s) 76, 152, 565
AspLEI GCGC 3 cut(s) 123, 138, 555
AspS9I GGNCC 5 cut(s) 93, 285, 418, 513, 751
AsuC2I CCSGG 1 cut(s) 366
AvaII GGWCC 3 cut(s) 93, 285, 751
AxyI CCTNAGG 1 cut(s) 587
BanII GRGCYC 2 cut(s) 33, 400
BarI GAAGNNNNNNTAC 2 cut(s) 740, 772
BbsI GAAGAC 2 cut(s) 157, 427
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 3 cut(s) 88, 139, 552
BcnI CCSGG 1 cut(s) 366
BfoI RGCGCY 1 cut(s) 124
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 9 cut(s) 9, 57, 60, 63, 77, 150, 153, 216, 566
BlsI GCNGC 9 cut(s) 10, 58, 61, 64, 78, 151, 154, 217, 567
Bme1390I CCNGG 1 cut(s) 366
Bme18I GGWCC 3 cut(s) 93, 285, 751
BmgT120I GGNCC 5 cut(s) 93, 285, 418, 513, 751
BmiI GGNNCC 3 cut(s) 12, 286, 419
BmrFI CCNGG 1 cut(s) 366
BmsI GCATC 2 cut(s) 638, 704
BpiI GAAGAC 2 cut(s) 157, 427
BplI GAGNNNNNCTC 2 cut(s) 580, 612
BpmI CTGGAG 1 cut(s) 471
Bpu10I CCTNAGC 1 cut(s) 32
BpuEI CTTGAG 2 cut(s) 218, 722
BpuMI CCSGG 1 cut(s) 366
BsaJI CCNNGG 2 cut(s) 51, 66
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse118I RCCGGY 1 cut(s) 98
Bse21I CCTNAGG 1 cut(s) 587
Bse3DI GCAATG 2 cut(s) 249, 383
BseDI CCNNGG 2 cut(s) 51, 66
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 1 cut(s) 427
BseMI GCAATG 2 cut(s) 249, 383
BseMII CTCAG 2 cut(s) 46, 578
BseRI GAGGAG 3 cut(s) 83, 86, 169
BseX3I CGGCCG 1 cut(s) 63
BseXI GCAGC 3 cut(s) 88, 139, 552
BseYI CCCAGC 1 cut(s) 138
Bsh1236I CGCG 1 cut(s) 136
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 8 cut(s) 65, 186, 392, 420, 493, 514, 684, 720
BsiEI CGRYCG 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 400
BsiSI CCGG 4 cut(s) 99, 183, 365, 393
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 1 cut(s) 427
BsmFI GGGAC 1 cut(s) 298
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 8 cut(s) 65, 186, 392, 420, 493, 514, 684, 720
Bsp1286I GDGCHC 2 cut(s) 33, 400
Bsp143I GATC 3 cut(s) 256, 274, 733
BspACI CCGC 7 cut(s) 8, 56, 59, 62, 150, 213, 216
BspANI GGCC 8 cut(s) 65, 186, 392, 420, 493, 514, 684, 720
BspCNI CTCAG 2 cut(s) 45, 579
BspFNI CGCG 1 cut(s) 136
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 3 cut(s) 12, 286, 419
BspPI GGATC 1 cut(s) 741
BsrDI GCAATG 2 cut(s) 249, 383
BsrFI RCCGGY 1 cut(s) 98
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 51, 66
BssMI GATC 3 cut(s) 256, 274, 733
BssT1I CCWWGG 1 cut(s) 51
Bst4CI ACNGT 3 cut(s) 227, 329, 709
Bst6I CTCTTC 2 cut(s) 36, 818
BstAPI GCANNNNNTGC 1 cut(s) 545
BstC8I GCNNGC 6 cut(s) 125, 213, 266, 390, 491, 718
BstDEI CTNAG 2 cut(s) 32, 587
BstF5I GGATG 1 cut(s) 43
BstFNI CGCG 1 cut(s) 136
BstH2I RGCGCY 1 cut(s) 124
BstHHI GCGC 3 cut(s) 123, 138, 555
BstKTI GATC 3 cut(s) 259, 277, 736
BstMBI GATC 3 cut(s) 256, 274, 733
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 5 cut(s) 56, 62, 133, 545, 722
BstSCI CCNGG 1 cut(s) 364
BstUI CGCG 1 cut(s) 136
BstV1I GCAGC 3 cut(s) 88, 139, 552
BstV2I GAAGAC 2 cut(s) 157, 427
BstX2I RGATCY 1 cut(s) 733
BstYI RGATCY 1 cut(s) 733
BstZI CGGCCG 1 cut(s) 63
Bsu36I CCTNAGG 1 cut(s) 587
BsuRI GGCC 8 cut(s) 65, 186, 392, 420, 493, 514, 684, 720
BtsCI GGATG 1 cut(s) 43
BtsIMutI CAGTG 2 cut(s) 750, 804
Cac8I GCNNGC 6 cut(s) 125, 213, 266, 390, 491, 718
CciI TCATGA 1 cut(s) 357
CfoI GCGC 3 cut(s) 123, 138, 555
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 5 cut(s) 93, 285, 418, 513, 751
CviAII CATG 4 cut(s) 208, 346, 358, 379
DdeI CTNAG 2 cut(s) 32, 587
DpnI GATC 3 cut(s) 258, 276, 735
DpnII GATC 3 cut(s) 256, 274, 733
DraI TTTAAA 1 cut(s) 766
EaeI YGGCCR 2 cut(s) 63, 390
EagI CGGCCG 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 36, 818
EarI CTCTTC 2 cut(s) 36, 818
Ecl136II GAGCTC 1 cut(s) 398
EclXI CGGCCG 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 51
Eco147I AGGCCT 1 cut(s) 493
Eco24I GRGCYC 2 cut(s) 33, 400
Eco47I GGWCC 3 cut(s) 93, 285, 751
Eco47III AGCGCT 1 cut(s) 122
Eco52I CGGCCG 1 cut(s) 63
Eco53kI GAGCTC 1 cut(s) 398
Eco81I CCTNAGG 1 cut(s) 587
EcoICRI GAGCTC 1 cut(s) 398
EcoO109I RGGNCCY 3 cut(s) 285, 418, 751
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 33, 400
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 4 cut(s) 211, 349, 361, 382
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FaqI GGGAC 1 cut(s) 298
FatI CATG 4 cut(s) 207, 345, 357, 378
FauI CCCGC 1 cut(s) 220
Fnu4HI GCNGC 9 cut(s) 9, 57, 60, 63, 77, 150, 153, 216, 566
FokI GGATG 1 cut(s) 50
FriOI GRGCYC 2 cut(s) 33, 400
Fsp4HI GCNGC 9 cut(s) 9, 57, 60, 63, 77, 150, 153, 216, 566
GlaI GCGC 3 cut(s) 122, 137, 554
GluI GCNGC 9 cut(s) 9, 57, 60, 63, 77, 150, 153, 216, 566
GsaI CCCAGC 1 cut(s) 142
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 124
HaeIII GGCC 8 cut(s) 65, 186, 392, 420, 493, 514, 684, 720
HapII CCGG 4 cut(s) 99, 183, 365, 393
HhaI GCGC 3 cut(s) 123, 138, 555
Hin1II CATG 4 cut(s) 211, 349, 361, 382
Hin6I GCGC 3 cut(s) 121, 136, 553
HinP1I GCGC 3 cut(s) 121, 136, 553
HincII GTYRAC 1 cut(s) 643
HindII GTYRAC 1 cut(s) 643
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 3 cut(s) 361, 528, 834
HpaII CCGG 4 cut(s) 99, 183, 365, 393
Hpy166II GTNNAC 3 cut(s) 96, 230, 643
Hpy188I TCNGA 3 cut(s) 15, 118, 713
Hpy188III TCNNGA 2 cut(s) 358, 586
Hpy8I GTNNAC 3 cut(s) 96, 230, 643
HpyAV CCTTC 2 cut(s) 431, 742
HpyCH4III ACNGT 3 cut(s) 227, 329, 709
HpyCH4V TGCA 4 cut(s) 293, 349, 548, 671
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 62, 133, 545, 722
HpyF3I CTNAG 2 cut(s) 32, 587
Hsp92II CATG 4 cut(s) 211, 349, 361, 382
HspAI GCGC 3 cut(s) 121, 136, 553
Kzo9I GATC 3 cut(s) 256, 274, 733
LmnI GCTCC 5 cut(s) 16, 73, 118, 395, 452
Lsp1109I GCAGC 3 cut(s) 88, 139, 552
LweI GCATC 2 cut(s) 638, 704
MaeIII GTNAC 1 cut(s) 323
MalI GATC 3 cut(s) 258, 276, 735
MboI GATC 3 cut(s) 256, 274, 733
MboII GAAGA 8 cut(s) 53, 157, 160, 251, 334, 427, 450, 835
MflI RGATCY 1 cut(s) 733
MhlI GDGCHC 2 cut(s) 33, 400
MluCI AATT 3 cut(s) 311, 483, 521
MmeI TCCRAC 3 cut(s) 96, 623, 836
MnlI CCTC 7 cut(s) 61, 64, 136, 140, 190, 582, 764
MseI TTAA 2 cut(s) 482, 765
MslI CAYNNNNRTG 1 cut(s) 344
MspA1I CMGCKG 3 cut(s) 152, 218, 449
MspI CCGG 4 cut(s) 99, 183, 365, 393
MspR9I CCNGG 1 cut(s) 366
Mva1269I GAATGC 1 cut(s) 52
MvnI CGCG 1 cut(s) 136
MwoI GCNNNNNNNGC 5 cut(s) 56, 62, 133, 545, 722
NciI CCSGG 1 cut(s) 366
NdeII GATC 3 cut(s) 256, 274, 733
NlaIII CATG 4 cut(s) 211, 349, 361, 382
NlaIV GGNNCC 3 cut(s) 12, 286, 419
NmeAIII GCCGAG 1 cut(s) 91
PagI TCATGA 1 cut(s) 357
PceI AGGCCT 1 cut(s) 493
PctI GAATGC 1 cut(s) 52
PfeI GAWTC 3 cut(s) 361, 528, 834
PfoI TCCNGGA 1 cut(s) 364
PkrI GCNGC 9 cut(s) 10, 58, 61, 64, 78, 151, 154, 217, 567
PpuMI RGGWCCY 2 cut(s) 285, 751
Psp124BI GAGCTC 1 cut(s) 400
Psp5II RGGWCCY 2 cut(s) 285, 751
PspFI CCCAGC 1 cut(s) 138
PspN4I GGNNCC 3 cut(s) 12, 286, 419
PspPI GGNCC 5 cut(s) 93, 285, 418, 513, 751
PspPPI RGGWCCY 2 cut(s) 285, 751
PsuI RGATCY 1 cut(s) 733
PvuII CAGCTG 1 cut(s) 449
RseI CAYNNNNRTG 1 cut(s) 344
SacI GAGCTC 1 cut(s) 400
SaqAI TTAA 2 cut(s) 482, 765
SatI GCNGC 9 cut(s) 9, 57, 60, 63, 77, 150, 153, 216, 566
Sau3AI GATC 3 cut(s) 256, 274, 733
Sau96I GGNCC 5 cut(s) 93, 285, 418, 513, 751
ScrFI CCNGG 1 cut(s) 366
SduI GDGCHC 2 cut(s) 33, 400
SfaNI GCATC 2 cut(s) 638, 704
SinI GGWCC 3 cut(s) 93, 285, 751
SmiMI CAYNNNNRTG 1 cut(s) 344
SmlI CTYRAG 2 cut(s) 233, 737
SmoI CTYRAG 2 cut(s) 233, 737
Sse9I AATT 3 cut(s) 311, 483, 521
SseBI AGGCCT 1 cut(s) 493
SsiI CCGC 7 cut(s) 8, 56, 59, 62, 150, 213, 216
SstI GAGCTC 1 cut(s) 400
StuI AGGCCT 1 cut(s) 493
StyD4I CCNGG 1 cut(s) 364
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 3 cut(s) 227, 329, 709
TasI AATT 3 cut(s) 311, 483, 521
TauI GCSGC 6 cut(s) 11, 59, 62, 65, 152, 218
TfiI GAWTC 3 cut(s) 361, 528, 834
Tru1I TTAA 2 cut(s) 482, 765
Tru9I TTAA 2 cut(s) 482, 765
TscAI CASTG 2 cut(s) 750, 804
TseI GCWGC 3 cut(s) 76, 152, 565
TspDTI ATGAA 7 cut(s) 54, 160, 304, 334, 346, 367, 607
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 2 cut(s) 750, 804
VpaK11BI GGWCC 3 cut(s) 93, 285, 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.