Rw6G015620

membrane protein At4g09580-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008356
Physical Location & Seq
Reverse (-)
30141974 .. 30146757
4784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G015620.1

Sequence Viewer

Length: 849 bp
ATGATGGCGGCTCCGAGAAATCTGGTGGGGCTCAGGGATGAAGAGAACGCCAAGGCGGCGGCGGCGGAGGACGACGACCAGCAGGACGATGATTGGTCCACCGGCAAGAAGCCCAAGTCGGAGCGCTTGCCCCTCTCCCGCTGGGAGGTCGCCGCTGCTCTCTCCGTCTTCTTCATATTCTCCTCCGGCCTTTTCTGTGTCTACTTGACCATGCCCGCCGCCGATTACGGTAAACTAAAGCTTCCACGCACCATTGCCGATCTTCGCTTGCTCAAAGATCATCTTGGGACCTATGCAAACGATTATCCAGCTCAATTCATTCTGGGTTACTGTTCCACATACATCTTCATGCAGACCTTCATGATTCCCGGAACGATATTCATGTCATTGCTGGCCGGAGCTCTTTTCGGGGTTTTCAGGGGCCTTCTCTTGGTTGTCTTCAACGCCACAGCTGGAGCTTCTTCCTGCTTCTTTTTGTCCAAGTTAATTGGCAGGCCTTTAGTCAGTTGGTTGTGGCCCGAAAAATTGAGATTCTTTCAGGCAGAGATCGCAAAGCGCAGGGAAAAGCTGCTCAACTATATGCTTTTCCTGAGGATAACTCCAACTTTGCCAAACCTTTTCATCAACTTGGCATCTCCCATTGTTGACATACCCTTTCACATTTTCTTTCTTGCAACTTTGATTGGTCTTATTCCAGCATCTTATATCACAGTCAGAGCTGGCCTTGCTCTTGGGGATCTCAAGTCAGTCAAGGACCTCTACGATTTTAAAACATTATTTGTGCTTTTCCTTATTGGTTCAGTGATTATATTTCCAACACTTTTGAAGAGAAAGCGAATCTATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

282

Amino Acids

31.78

Weight (kDa)

9.26

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 122 - 241 1.7e-15 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 201
AciI CCGC 9 cut(s) 8, 56, 59, 62, 65, 139, 153, 216, 219
AclWI GGATC 1 cut(s) 744
AcoI YGGCCR 1 cut(s) 393
AfeI AGCGCT 1 cut(s) 125
AfiI CCNNNNNNNGG 2 cut(s) 145, 430
AgsI TTSAA 2 cut(s) 442, 826
AluBI AGCT 7 cut(s) 241, 311, 401, 452, 458, 568, 719
AluI AGCT 7 cut(s) 241, 311, 401, 452, 458, 568, 719
Alw21I GWGCWC 1 cut(s) 403
AlwI GGATC 1 cut(s) 744
Aor51HI AGCGCT 1 cut(s) 125
AoxI GGCC 6 cut(s) 187, 393, 421, 494, 515, 721
ApeKI GCWGC 2 cut(s) 155, 568
AspLEI GCGC 2 cut(s) 126, 558
AspS9I GGNCC 5 cut(s) 96, 288, 421, 516, 754
AsuC2I CCSGG 1 cut(s) 369
AvaII GGWCC 3 cut(s) 96, 288, 754
AxyI CCTNAGG 1 cut(s) 590
BanII GRGCYC 2 cut(s) 33, 403
BbsI GAAGAC 2 cut(s) 160, 430
Bbv12I GWGCWC 1 cut(s) 403
BbvI GCAGC 2 cut(s) 142, 555
BcnI CCSGG 1 cut(s) 369
BfoI RGCGCY 1 cut(s) 127
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 8 cut(s) 9, 57, 60, 63, 153, 156, 219, 569
BlsI GCNGC 8 cut(s) 10, 58, 61, 64, 154, 157, 220, 570
Bme1390I CCNGG 1 cut(s) 369
Bme18I GGWCC 3 cut(s) 96, 288, 754
BmgT120I GGNCC 5 cut(s) 96, 288, 421, 516, 754
BmiI GGNNCC 3 cut(s) 12, 289, 422
BmrFI CCNGG 1 cut(s) 369
BmsI GCATC 2 cut(s) 641, 707
BpiI GAAGAC 2 cut(s) 160, 430
BplI GAGNNNNNCTC 2 cut(s) 583, 615
BpmI CTGGAG 1 cut(s) 474
Bpu10I CCTNAGC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 725
BpuMI CCSGG 1 cut(s) 369
BsaJI CCNNGG 1 cut(s) 51
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 430
Bse118I RCCGGY 1 cut(s) 101
Bse21I CCTNAGG 1 cut(s) 590
Bse3DI GCAATG 2 cut(s) 252, 386
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 2 cut(s) 145, 430
BseMI GCAATG 2 cut(s) 252, 386
BseMII CTCAG 2 cut(s) 46, 581
BseRI GAGGAG 1 cut(s) 172
BseXI GCAGC 2 cut(s) 142, 555
BseYI CCCAGC 1 cut(s) 141
BshFI GGCC 6 cut(s) 189, 395, 423, 496, 517, 723
BsiHKAI GWGCWC 1 cut(s) 403
BsiSI CCGG 4 cut(s) 102, 186, 369, 396
BslFI GGGAC 1 cut(s) 301
BslI CCNNNNNNNGG 2 cut(s) 145, 430
BsmFI GGGAC 1 cut(s) 301
BsnI GGCC 6 cut(s) 189, 395, 423, 496, 517, 723
Bsp1286I GDGCHC 2 cut(s) 33, 403
Bsp143I GATC 4 cut(s) 259, 277, 546, 736
BspACI CCGC 9 cut(s) 8, 56, 59, 62, 65, 139, 153, 216, 219
BspANI GGCC 6 cut(s) 189, 395, 423, 496, 517, 723
BspCNI CTCAG 2 cut(s) 45, 582
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 3 cut(s) 12, 289, 422
BspPI GGATC 1 cut(s) 744
BsrDI GCAATG 2 cut(s) 252, 386
BsrFI RCCGGY 1 cut(s) 101
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 4 cut(s) 259, 277, 546, 736
BssT1I CCWWGG 1 cut(s) 51
Bst4CI ACNGT 3 cut(s) 230, 332, 712
Bst6I CTCTTC 2 cut(s) 36, 821
BstC8I GCNNGC 6 cut(s) 128, 216, 269, 393, 494, 721
BstDEI CTNAG 2 cut(s) 32, 590
BstF5I GGATG 1 cut(s) 43
BstH2I RGCGCY 1 cut(s) 127
BstHHI GCGC 2 cut(s) 126, 558
BstKTI GATC 4 cut(s) 262, 280, 549, 739
BstMBI GATC 4 cut(s) 259, 277, 546, 736
BstMWI GCNNNNNNNGC 4 cut(s) 56, 62, 548, 725
BstSCI CCNGG 1 cut(s) 367
BstV1I GCAGC 2 cut(s) 142, 555
BstV2I GAAGAC 2 cut(s) 160, 430
BstX2I RGATCY 1 cut(s) 736
BstYI RGATCY 1 cut(s) 736
Bsu36I CCTNAGG 1 cut(s) 590
BsuRI GGCC 6 cut(s) 189, 395, 423, 496, 517, 723
BtsCI GGATG 1 cut(s) 43
BtsIMutI CAGTG 1 cut(s) 807
Cac8I GCNNGC 6 cut(s) 128, 216, 269, 393, 494, 721
CciI TCATGA 1 cut(s) 360
CfoI GCGC 2 cut(s) 126, 558
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 5 cut(s) 96, 288, 421, 516, 754
CviAII CATG 4 cut(s) 211, 349, 361, 382
DdeI CTNAG 2 cut(s) 32, 590
DpnI GATC 4 cut(s) 261, 279, 548, 738
DpnII GATC 4 cut(s) 259, 277, 546, 736
DraI TTTAAA 1 cut(s) 769
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 2 cut(s) 36, 821
EarI CTCTTC 2 cut(s) 36, 821
EciI GGCGGA 1 cut(s) 80
Ecl136II GAGCTC 1 cut(s) 401
Eco130I CCWWGG 1 cut(s) 51
Eco147I AGGCCT 1 cut(s) 496
Eco24I GRGCYC 2 cut(s) 33, 403
Eco47I GGWCC 3 cut(s) 96, 288, 754
Eco47III AGCGCT 1 cut(s) 125
Eco53kI GAGCTC 1 cut(s) 401
Eco81I CCTNAGG 1 cut(s) 590
EcoICRI GAGCTC 1 cut(s) 401
EcoO109I RGGNCCY 3 cut(s) 288, 421, 754
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 33, 403
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 4 cut(s) 214, 352, 364, 385
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FaqI GGGAC 1 cut(s) 301
FatI CATG 4 cut(s) 210, 348, 360, 381
FauI CCCGC 2 cut(s) 146, 223
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 8 cut(s) 9, 57, 60, 63, 153, 156, 219, 569
FokI GGATG 1 cut(s) 50
FriOI GRGCYC 2 cut(s) 33, 403
Fsp4HI GCNGC 8 cut(s) 9, 57, 60, 63, 153, 156, 219, 569
GlaI GCGC 2 cut(s) 125, 557
GluI GCNGC 8 cut(s) 9, 57, 60, 63, 153, 156, 219, 569
GsaI CCCAGC 1 cut(s) 145
GsuI CTGGAG 1 cut(s) 474
HaeII RGCGCY 1 cut(s) 127
HaeIII GGCC 6 cut(s) 189, 395, 423, 496, 517, 723
HapII CCGG 4 cut(s) 102, 186, 369, 396
HhaI GCGC 2 cut(s) 126, 558
Hin1II CATG 4 cut(s) 214, 352, 364, 385
Hin6I GCGC 2 cut(s) 124, 556
HinP1I GCGC 2 cut(s) 124, 556
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 3 cut(s) 364, 531, 837
HpaII CCGG 4 cut(s) 102, 186, 369, 396
Hpy166II GTNNAC 4 cut(s) 99, 202, 233, 646
Hpy188I TCNGA 3 cut(s) 15, 121, 716
Hpy188III TCNNGA 2 cut(s) 361, 589
Hpy8I GTNNAC 4 cut(s) 99, 202, 233, 646
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 2 cut(s) 367, 434
HpyCH4III ACNGT 3 cut(s) 230, 332, 712
HpyCH4V TGCA 3 cut(s) 296, 352, 674
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 62, 548, 725
HpyF3I CTNAG 2 cut(s) 32, 590
Hsp92II CATG 4 cut(s) 214, 352, 364, 385
HspAI GCGC 2 cut(s) 124, 556
Kzo9I GATC 4 cut(s) 259, 277, 546, 736
LmnI GCTCC 4 cut(s) 16, 121, 398, 455
Lsp1109I GCAGC 2 cut(s) 142, 555
LweI GCATC 2 cut(s) 641, 707
MaeIII GTNAC 1 cut(s) 326
MalI GATC 4 cut(s) 261, 279, 548, 738
MboI GATC 4 cut(s) 259, 277, 546, 736
MboII GAAGA 8 cut(s) 53, 160, 163, 254, 337, 430, 453, 838
MflI RGATCY 1 cut(s) 736
MhlI GDGCHC 2 cut(s) 33, 403
MluCI AATT 3 cut(s) 314, 486, 524
MmeI TCCRAC 3 cut(s) 99, 626, 839
MnlI CCTC 6 cut(s) 61, 139, 143, 193, 585, 767
MseI TTAA 2 cut(s) 485, 768
MslI CAYNNNNRTG 1 cut(s) 347
MspA1I CMGCKG 3 cut(s) 141, 155, 452
MspI CCGG 4 cut(s) 102, 186, 369, 396
MspR9I CCNGG 1 cut(s) 369
MwoI GCNNNNNNNGC 4 cut(s) 56, 62, 548, 725
NciI CCSGG 1 cut(s) 369
NdeII GATC 4 cut(s) 259, 277, 546, 736
NlaIII CATG 4 cut(s) 214, 352, 364, 385
NlaIV GGNNCC 3 cut(s) 12, 289, 422
PagI TCATGA 1 cut(s) 360
PceI AGGCCT 1 cut(s) 496
PfeI GAWTC 3 cut(s) 364, 531, 837
PfoI TCCNGGA 1 cut(s) 367
PkrI GCNGC 8 cut(s) 10, 58, 61, 64, 154, 157, 220, 570
PpuMI RGGWCCY 2 cut(s) 288, 754
Psp124BI GAGCTC 1 cut(s) 403
Psp5II RGGWCCY 2 cut(s) 288, 754
PspFI CCCAGC 1 cut(s) 141
PspN4I GGNNCC 3 cut(s) 12, 289, 422
PspPI GGNCC 5 cut(s) 96, 288, 421, 516, 754
PspPPI RGGWCCY 2 cut(s) 288, 754
PsuI RGATCY 1 cut(s) 736
PvuII CAGCTG 1 cut(s) 452
RseI CAYNNNNRTG 1 cut(s) 347
SacI GAGCTC 1 cut(s) 403
SaqAI TTAA 2 cut(s) 485, 768
SatI GCNGC 8 cut(s) 9, 57, 60, 63, 153, 156, 219, 569
Sau3AI GATC 4 cut(s) 259, 277, 546, 736
Sau96I GGNCC 5 cut(s) 96, 288, 421, 516, 754
ScrFI CCNGG 1 cut(s) 369
SduI GDGCHC 2 cut(s) 33, 403
SfaNI GCATC 2 cut(s) 641, 707
SinI GGWCC 3 cut(s) 96, 288, 754
SmiMI CAYNNNNRTG 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 3 cut(s) 314, 486, 524
SseBI AGGCCT 1 cut(s) 496
SsiI CCGC 9 cut(s) 8, 56, 59, 62, 65, 139, 153, 216, 219
SstI GAGCTC 1 cut(s) 403
StuI AGGCCT 1 cut(s) 496
StyD4I CCNGG 1 cut(s) 367
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 3 cut(s) 230, 332, 712
TasI AATT 3 cut(s) 314, 486, 524
TauI GCSGC 6 cut(s) 11, 59, 62, 65, 155, 221
TfiI GAWTC 3 cut(s) 364, 531, 837
Tru1I TTAA 2 cut(s) 485, 768
Tru9I TTAA 2 cut(s) 485, 768
TscAI CASTG 1 cut(s) 807
TseI GCWGC 2 cut(s) 155, 568
TspDTI ATGAA 7 cut(s) 54, 163, 307, 337, 349, 370, 610
TspGWI ACGGA 1 cut(s) 154
TspRI CASTG 1 cut(s) 807
VpaK11BI GGWCC 3 cut(s) 96, 288, 754
XmiI GTMKAC 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.