RchiOBHm_Chr6g0272001

membrane protein At4g09580-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008356
Physical Location & Seq
Forward (+)
32074347 .. 32078850
4504 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24399

Sequence Viewer

Length: 852 bp
ATGATGGCGGCTCCGAGAAATCTGGTGGGGCTCAGGGATGAAGAGAATGCCAAGGCGGCGGCGGCGGCGGAGGAGGACGACCACCAGGACGATGATTGGTCCACCGGCAAGAAGCCCAAGTCGGAGCGCTTGCCCCTCTCCCGCTGGGAGGTCGCCGCTGCTCTCTCCGTCTTCTTCATATTCTCCTCCGGCCTTTTCTGTGTCTACTTGACCATGCCCGCCGCCGATTACGGTAAACTCAAGCTTCCACGCACCATTGCCGATCTTCGCTTGCTCAAAGATCATCTTGGGACCTATGCAAACGATTATCCAGCTCAATTCATTCTGGGTTACTGTTCCACATACATCTTCATGCAGACTTTCATGATTCCCGGAACGATATTCATGTCATTGCTCGCCGGAGCTCTTTTCGGGGTTTTCAGGGGCCTTCTCCTGGTTGTCTTCAACGCCACTGCTGGAGCTTCTTCCTGCTTCTTTTTGTCCAAGTTAATTGGCAGGCCTTTAGTCAGTTGGTTGTGGCCCGAAAAATTGAGATTCTTTCAGGCAGAGATCGCAAAGCGCAGGGAAAAGCTGCTCAACTATATGCTTTTCCTGAGGATAACTCCAACATTGCCAAACCTTTTCATCAACTTGGCATCTCCCATTGTTGACATACCCTTTCACATTTTCTTTCTTGCAACTTTGATTGGTCTTATTCCAGCATCTTATATCACAGTCAGAGCTGGCCTTGCTCTTGGGGATCTCAAGTCAGTCAAGGACCTCTACGATTTTAAAACATTATTTGTGCTTTTCCTTATTGGTTCAGTGATTATATTTCCAACACTTTTGAAGAGAAAGCGAATCTATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

283

Amino Acids

31.87

Weight (kDa)

9.26

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 123 - 242 1.8e-15 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 204
AclWI GGATC 1 cut(s) 747
AfeI AGCGCT 1 cut(s) 128
AfiI CCNNNNNNNGG 2 cut(s) 148, 433
AgsI TTSAA 2 cut(s) 445, 829
AjnI CCWGG 2 cut(s) 84, 432
AluBI AGCT 6 cut(s) 244, 314, 404, 461, 571, 722
AluI AGCT 6 cut(s) 244, 314, 404, 461, 571, 722
Alw21I GWGCWC 1 cut(s) 406
AlwI GGATC 1 cut(s) 747
Aor51HI AGCGCT 1 cut(s) 128
AoxI GGCC 5 cut(s) 190, 424, 497, 518, 724
ApeKI GCWGC 2 cut(s) 158, 571
AspLEI GCGC 2 cut(s) 129, 561
AspS9I GGNCC 5 cut(s) 99, 291, 424, 519, 757
AsuC2I CCSGG 1 cut(s) 372
AvaII GGWCC 3 cut(s) 99, 291, 757
AxyI CCTNAGG 1 cut(s) 593
BanII GRGCYC 2 cut(s) 33, 406
BbsI GAAGAC 2 cut(s) 163, 433
Bbv12I GWGCWC 1 cut(s) 406
BbvI GCAGC 2 cut(s) 145, 558
BciT130I CCWGG 2 cut(s) 86, 434
BcnI CCSGG 1 cut(s) 372
BfoI RGCGCY 1 cut(s) 130
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 9 cut(s) 9, 57, 60, 63, 66, 156, 159, 222, 572
BlsI GCNGC 9 cut(s) 10, 58, 61, 64, 67, 157, 160, 223, 573
Bme1390I CCNGG 3 cut(s) 86, 372, 434
Bme18I GGWCC 3 cut(s) 99, 291, 757
BmgT120I GGNCC 5 cut(s) 99, 291, 424, 519, 757
BmiI GGNNCC 3 cut(s) 12, 292, 425
BmrFI CCNGG 3 cut(s) 86, 372, 434
BmsI GCATC 2 cut(s) 644, 710
BpiI GAAGAC 2 cut(s) 163, 433
BplI GAGNNNNNCTC 2 cut(s) 586, 618
BpmI CTGGAG 1 cut(s) 477
Bpu10I CCTNAGC 1 cut(s) 32
BpuEI CTTGAG 2 cut(s) 224, 728
BpuMI CCSGG 1 cut(s) 372
BsaJI CCNNGG 1 cut(s) 51
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 433
Bse118I RCCGGY 1 cut(s) 104
Bse21I CCTNAGG 1 cut(s) 593
Bse3DI GCAATG 3 cut(s) 255, 389, 608
BseBI CCWGG 2 cut(s) 86, 434
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 2 cut(s) 148, 433
BseMI GCAATG 3 cut(s) 255, 389, 608
BseMII CTCAG 2 cut(s) 46, 584
BseRI GAGGAG 2 cut(s) 86, 175
BseXI GCAGC 2 cut(s) 145, 558
BseYI CCCAGC 1 cut(s) 144
BshFI GGCC 5 cut(s) 192, 426, 499, 520, 726
BsiHKAI GWGCWC 1 cut(s) 406
BsiSI CCGG 4 cut(s) 105, 189, 372, 399
BslFI GGGAC 1 cut(s) 304
BslI CCNNNNNNNGG 2 cut(s) 148, 433
BsmFI GGGAC 1 cut(s) 304
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 5 cut(s) 192, 426, 499, 520, 726
Bsp1286I GDGCHC 2 cut(s) 33, 406
Bsp143I GATC 4 cut(s) 262, 280, 549, 739
BspANI GGCC 5 cut(s) 192, 426, 499, 520, 726
BspCNI CTCAG 2 cut(s) 45, 585
BspHI TCATGA 1 cut(s) 363
BspLI GGNNCC 3 cut(s) 12, 292, 425
BspPI GGATC 1 cut(s) 747
BsrDI GCAATG 3 cut(s) 255, 389, 608
BsrFI RCCGGY 1 cut(s) 104
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 4 cut(s) 262, 280, 549, 739
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 2 cut(s) 86, 434
Bst4CI ACNGT 3 cut(s) 233, 335, 715
Bst6I CTCTTC 2 cut(s) 36, 824
BstC8I GCNNGC 6 cut(s) 131, 219, 272, 396, 497, 724
BstDEI CTNAG 2 cut(s) 32, 593
BstF5I GGATG 1 cut(s) 43
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 2 cut(s) 129, 561
BstKTI GATC 4 cut(s) 265, 283, 552, 742
BstMBI GATC 4 cut(s) 262, 280, 549, 739
BstMWI GCNNNNNNNGC 5 cut(s) 56, 62, 65, 551, 728
BstNI CCWGG 2 cut(s) 86, 434
BstSCI CCNGG 3 cut(s) 84, 370, 432
BstV1I GCAGC 2 cut(s) 145, 558
BstV2I GAAGAC 2 cut(s) 163, 433
BstX2I RGATCY 1 cut(s) 739
BstYI RGATCY 1 cut(s) 739
Bsu36I CCTNAGG 1 cut(s) 593
BsuRI GGCC 5 cut(s) 192, 426, 499, 520, 726
BtsCI GGATG 1 cut(s) 43
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 2 cut(s) 450, 810
Cac8I GCNNGC 6 cut(s) 131, 219, 272, 396, 497, 724
CciI TCATGA 1 cut(s) 363
CfoI GCGC 2 cut(s) 129, 561
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 5 cut(s) 99, 291, 424, 519, 757
CviAII CATG 4 cut(s) 214, 352, 364, 385
DdeI CTNAG 2 cut(s) 32, 593
DpnI GATC 4 cut(s) 264, 282, 551, 741
DpnII GATC 4 cut(s) 262, 280, 549, 739
DraI TTTAAA 1 cut(s) 772
Eam1104I CTCTTC 2 cut(s) 36, 824
EarI CTCTTC 2 cut(s) 36, 824
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 404
Eco130I CCWWGG 1 cut(s) 51
Eco147I AGGCCT 1 cut(s) 499
Eco24I GRGCYC 2 cut(s) 33, 406
Eco47I GGWCC 3 cut(s) 99, 291, 757
Eco47III AGCGCT 1 cut(s) 128
Eco53kI GAGCTC 1 cut(s) 404
Eco81I CCTNAGG 1 cut(s) 593
EcoICRI GAGCTC 1 cut(s) 404
EcoO109I RGGNCCY 3 cut(s) 291, 424, 757
EcoRII CCWGG 2 cut(s) 84, 432
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 33, 406
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 4 cut(s) 217, 355, 367, 388
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FaqI GGGAC 1 cut(s) 304
FatI CATG 4 cut(s) 213, 351, 363, 384
FauI CCCGC 2 cut(s) 149, 226
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 9 cut(s) 9, 57, 60, 63, 66, 156, 159, 222, 572
FokI GGATG 1 cut(s) 50
FriOI GRGCYC 2 cut(s) 33, 406
Fsp4HI GCNGC 9 cut(s) 9, 57, 60, 63, 66, 156, 159, 222, 572
GlaI GCGC 2 cut(s) 128, 560
GluI GCNGC 9 cut(s) 9, 57, 60, 63, 66, 156, 159, 222, 572
GsaI CCCAGC 1 cut(s) 148
GsuI CTGGAG 1 cut(s) 477
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 5 cut(s) 192, 426, 499, 520, 726
HapII CCGG 4 cut(s) 105, 189, 372, 399
HhaI GCGC 2 cut(s) 129, 561
Hin1II CATG 4 cut(s) 217, 355, 367, 388
Hin6I GCGC 2 cut(s) 127, 559
HinP1I GCGC 2 cut(s) 127, 559
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HindIII AAGCTT 1 cut(s) 242
HinfI GANTC 3 cut(s) 367, 534, 840
HpaII CCGG 4 cut(s) 105, 189, 372, 399
Hpy166II GTNNAC 4 cut(s) 102, 205, 236, 649
Hpy188I TCNGA 3 cut(s) 15, 124, 719
Hpy188III TCNNGA 2 cut(s) 364, 592
Hpy8I GTNNAC 4 cut(s) 102, 205, 236, 649
HpyAV CCTTC 1 cut(s) 437
HpyCH4III ACNGT 3 cut(s) 233, 335, 715
HpyCH4V TGCA 3 cut(s) 299, 355, 677
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 62, 65, 551, 728
HpyF3I CTNAG 2 cut(s) 32, 593
Hsp92II CATG 4 cut(s) 217, 355, 367, 388
HspAI GCGC 2 cut(s) 127, 559
Kzo9I GATC 4 cut(s) 262, 280, 549, 739
LmnI GCTCC 4 cut(s) 16, 124, 401, 458
Lsp1109I GCAGC 2 cut(s) 145, 558
LweI GCATC 2 cut(s) 644, 710
MaeIII GTNAC 1 cut(s) 329
MalI GATC 4 cut(s) 264, 282, 551, 741
MboI GATC 4 cut(s) 262, 280, 549, 739
MboII GAAGA 8 cut(s) 53, 163, 166, 257, 340, 433, 456, 841
MflI RGATCY 1 cut(s) 739
MhlI GDGCHC 2 cut(s) 33, 406
MluCI AATT 3 cut(s) 317, 489, 527
MmeI TCCRAC 3 cut(s) 102, 629, 842
MnlI CCTC 7 cut(s) 64, 67, 142, 146, 196, 588, 770
MseI TTAA 2 cut(s) 488, 771
MslI CAYNNNNRTG 1 cut(s) 350
MspA1I CMGCKG 2 cut(s) 144, 158
MspI CCGG 4 cut(s) 105, 189, 372, 399
MspR9I CCNGG 3 cut(s) 86, 372, 434
Mva1269I GAATGC 1 cut(s) 52
MvaI CCWGG 2 cut(s) 86, 434
MwoI GCNNNNNNNGC 5 cut(s) 56, 62, 65, 551, 728
NciI CCSGG 1 cut(s) 372
NdeII GATC 4 cut(s) 262, 280, 549, 739
NlaIII CATG 4 cut(s) 217, 355, 367, 388
NlaIV GGNNCC 3 cut(s) 12, 292, 425
PagI TCATGA 1 cut(s) 363
PceI AGGCCT 1 cut(s) 499
PctI GAATGC 1 cut(s) 52
PfeI GAWTC 3 cut(s) 367, 534, 840
PfoI TCCNGGA 1 cut(s) 370
PkrI GCNGC 9 cut(s) 10, 58, 61, 64, 67, 157, 160, 223, 573
PpuMI RGGWCCY 2 cut(s) 291, 757
Psp124BI GAGCTC 1 cut(s) 406
Psp5II RGGWCCY 2 cut(s) 291, 757
Psp6I CCWGG 2 cut(s) 84, 432
PspFI CCCAGC 1 cut(s) 144
PspGI CCWGG 2 cut(s) 84, 432
PspN4I GGNNCC 3 cut(s) 12, 292, 425
PspPI GGNCC 5 cut(s) 99, 291, 424, 519, 757
PspPPI RGGWCCY 2 cut(s) 291, 757
PsuI RGATCY 1 cut(s) 739
RseI CAYNNNNRTG 1 cut(s) 350
SacI GAGCTC 1 cut(s) 406
SaqAI TTAA 2 cut(s) 488, 771
SatI GCNGC 9 cut(s) 9, 57, 60, 63, 66, 156, 159, 222, 572
Sau3AI GATC 4 cut(s) 262, 280, 549, 739
Sau96I GGNCC 5 cut(s) 99, 291, 424, 519, 757
ScrFI CCNGG 3 cut(s) 86, 372, 434
SduI GDGCHC 2 cut(s) 33, 406
SfaNI GCATC 2 cut(s) 644, 710
SinI GGWCC 3 cut(s) 99, 291, 757
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 2 cut(s) 239, 743
SmoI CTYRAG 2 cut(s) 239, 743
Sse9I AATT 3 cut(s) 317, 489, 527
SseBI AGGCCT 1 cut(s) 499
SstI GAGCTC 1 cut(s) 406
StuI AGGCCT 1 cut(s) 499
StyD4I CCNGG 3 cut(s) 84, 370, 432
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 3 cut(s) 233, 335, 715
TasI AATT 3 cut(s) 317, 489, 527
TauI GCSGC 7 cut(s) 11, 59, 62, 65, 68, 158, 224
TfiI GAWTC 3 cut(s) 367, 534, 840
Tru1I TTAA 2 cut(s) 488, 771
Tru9I TTAA 2 cut(s) 488, 771
TscAI CASTG 2 cut(s) 457, 810
TseI GCWGC 2 cut(s) 158, 571
TspDTI ATGAA 7 cut(s) 54, 166, 310, 340, 352, 373, 613
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 2 cut(s) 457, 810
VpaK11BI GGWCC 3 cut(s) 99, 291, 757
XmiI GTMKAC 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.