Rw6G015680

membrane protein At4g09580-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008356
Physical Location & Seq
Forward (+)
30355412 .. 30359746
4335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G015680.1

Sequence Viewer

Length: 846 bp
ATGGCGGCTCCGAGAAATCTGGTGGGGCTCAGGGATGAAGAGAATGCCAAGGCGGCGGCGGCGGAGGACGACGACCAGCAGGACGATGATTGGTCCACCGGCAAGAAGCCCAAGTCGGAGCGCTTGCCCCTCTCCCGCTGGGAGGTCGCCGCTGCTGTCTCCGTCTTCTTCATATTCTCCTCCGGCCTTTTCTGTGTCTACTTGACCATGCCCGCCGCCGATTACGGTAAACTCAAGCTTCCACGCACCATTGCCGATCTTCGCTTGCTCAAAGATCATCTTGGCACCTATGCAAACGATTATCCAGCTCAATTCATTCTGGGTTACTGTTCCACATACATCTTCATGCAGACTTTCATGATTCCCGGAACGATATTCATGTCATTGCTGGCCGGAGCTCTTTTCGGGGTTTTCAGGGGCCTTCTCTTGGTTGTCTTCAACGCCACAGCTGGAGCTTCTTCCTGCTTCTTTTTGTCCAAGTTAATTGGCAGGCCTTTAGTCAGTTGGTTGTGGCCCGAAAAATTGAGATTCTTTCAGGCAGAGATCGCAAAGCGCAGGGAAAAGCTGCTCAACTATATGCTTTTCCTGAGGATAACTCCAACATTGCCAAACCTTTTCATCAACTTGGCATCTCCCATTGTTGACATACCCTTTCACATTTTCTTTCTTGCAACTTTGATTGGTCTTATTCCAGCATCTTATATCACAGTCAGAGCTGGCCTTGCTCTTGGGGATCTCAAGTCAGTCAAGGACCTCTACGATTTTAAAACATTATTTGTGCTTTTCCTTATTGGTTCAGTGATTATATTTCCAACACTTTTGAAGAGAAAGCGAATCTATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

281

Amino Acids

31.63

Weight (kDa)

9.26

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 121 - 240 1.7e-15 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 284
AccI GTMKAC 1 cut(s) 198
AciI CCGC 9 cut(s) 5, 53, 56, 59, 62, 136, 150, 213, 216
AclWI GGATC 1 cut(s) 741
AcoI YGGCCR 1 cut(s) 390
AfeI AGCGCT 1 cut(s) 122
AfiI CCNNNNNNNGG 2 cut(s) 142, 427
AgsI TTSAA 2 cut(s) 439, 823
AluBI AGCT 7 cut(s) 238, 308, 398, 449, 455, 565, 716
AluI AGCT 7 cut(s) 238, 308, 398, 449, 455, 565, 716
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 1 cut(s) 741
Aor51HI AGCGCT 1 cut(s) 122
AoxI GGCC 6 cut(s) 184, 390, 418, 491, 512, 718
ApeKI GCWGC 2 cut(s) 152, 565
AspLEI GCGC 2 cut(s) 123, 555
AspS9I GGNCC 4 cut(s) 93, 418, 513, 751
AsuC2I CCSGG 1 cut(s) 366
AvaII GGWCC 2 cut(s) 93, 751
AxyI CCTNAGG 1 cut(s) 587
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 2 cut(s) 30, 400
BbsI GAAGAC 2 cut(s) 157, 427
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 2 cut(s) 139, 552
BcnI CCSGG 1 cut(s) 366
BcoDI GTCTC 1 cut(s) 163
BfoI RGCGCY 1 cut(s) 124
BglI GCCNNNNNGGC 1 cut(s) 53
BisI GCNGC 8 cut(s) 6, 54, 57, 60, 150, 153, 216, 566
BlsI GCNGC 8 cut(s) 7, 55, 58, 61, 151, 154, 217, 567
Bme1390I CCNGG 1 cut(s) 366
Bme18I GGWCC 2 cut(s) 93, 751
BmgT120I GGNCC 4 cut(s) 93, 418, 513, 751
BmiI GGNNCC 3 cut(s) 9, 286, 419
BmrFI CCNGG 1 cut(s) 366
BmsI GCATC 2 cut(s) 638, 704
BpiI GAAGAC 2 cut(s) 157, 427
BplI GAGNNNNNCTC 2 cut(s) 580, 612
BpmI CTGGAG 1 cut(s) 471
Bpu10I CCTNAGC 1 cut(s) 29
BpuEI CTTGAG 2 cut(s) 218, 722
BpuMI CCSGG 1 cut(s) 366
BsaJI CCNNGG 1 cut(s) 48
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 427
Bse118I RCCGGY 1 cut(s) 98
Bse21I CCTNAGG 1 cut(s) 587
Bse3DI GCAATG 3 cut(s) 249, 383, 602
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 1 cut(s) 40
BseLI CCNNNNNNNGG 2 cut(s) 142, 427
BseMI GCAATG 3 cut(s) 249, 383, 602
BseMII CTCAG 2 cut(s) 43, 578
BseRI GAGGAG 1 cut(s) 169
BseXI GCAGC 2 cut(s) 139, 552
BseYI CCCAGC 1 cut(s) 138
BshFI GGCC 6 cut(s) 186, 392, 420, 493, 514, 720
BshNI GGYRCC 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 400
BsiSI CCGG 4 cut(s) 99, 183, 366, 393
BslI CCNNNNNNNGG 2 cut(s) 142, 427
BsmAI GTCTC 1 cut(s) 163
BsmI GAATGC 1 cut(s) 49
BsnI GGCC 6 cut(s) 186, 392, 420, 493, 514, 720
Bsp1286I GDGCHC 2 cut(s) 30, 400
Bsp143I GATC 4 cut(s) 256, 274, 543, 733
BspACI CCGC 9 cut(s) 5, 53, 56, 59, 62, 136, 150, 213, 216
BspANI GGCC 6 cut(s) 186, 392, 420, 493, 514, 720
BspCNI CTCAG 2 cut(s) 42, 579
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 3 cut(s) 9, 286, 419
BspPI GGATC 1 cut(s) 741
BspT107I GGYRCC 1 cut(s) 284
BsrDI GCAATG 3 cut(s) 249, 383, 602
BsrFI RCCGGY 1 cut(s) 98
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 4 cut(s) 256, 274, 543, 733
BssT1I CCWWGG 1 cut(s) 48
Bst4CI ACNGT 3 cut(s) 227, 329, 709
Bst6I CTCTTC 2 cut(s) 33, 818
BstC8I GCNNGC 6 cut(s) 125, 213, 266, 390, 491, 718
BstDEI CTNAG 2 cut(s) 29, 587
BstF5I GGATG 1 cut(s) 40
BstH2I RGCGCY 1 cut(s) 124
BstHHI GCGC 2 cut(s) 123, 555
BstKTI GATC 4 cut(s) 259, 277, 546, 736
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 4 cut(s) 256, 274, 543, 733
BstMWI GCNNNNNNNGC 4 cut(s) 53, 59, 545, 722
BstSCI CCNGG 1 cut(s) 364
BstV1I GCAGC 2 cut(s) 139, 552
BstV2I GAAGAC 2 cut(s) 157, 427
BstX2I RGATCY 1 cut(s) 733
BstYI RGATCY 1 cut(s) 733
Bsu36I CCTNAGG 1 cut(s) 587
BsuRI GGCC 6 cut(s) 186, 392, 420, 493, 514, 720
BtsCI GGATG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 804
Cac8I GCNNGC 6 cut(s) 125, 213, 266, 390, 491, 718
CciI TCATGA 1 cut(s) 357
CfoI GCGC 2 cut(s) 123, 555
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 4 cut(s) 93, 418, 513, 751
CviAII CATG 4 cut(s) 208, 346, 358, 379
DdeI CTNAG 2 cut(s) 29, 587
DpnI GATC 4 cut(s) 258, 276, 545, 735
DpnII GATC 4 cut(s) 256, 274, 543, 733
DraI TTTAAA 1 cut(s) 766
EaeI YGGCCR 1 cut(s) 390
Eam1104I CTCTTC 2 cut(s) 33, 818
EarI CTCTTC 2 cut(s) 33, 818
EciI GGCGGA 1 cut(s) 77
Ecl136II GAGCTC 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 48
Eco147I AGGCCT 1 cut(s) 493
Eco24I GRGCYC 2 cut(s) 30, 400
Eco47I GGWCC 2 cut(s) 93, 751
Eco47III AGCGCT 1 cut(s) 122
Eco53kI GAGCTC 1 cut(s) 398
Eco81I CCTNAGG 1 cut(s) 587
EcoICRI GAGCTC 1 cut(s) 398
EcoO109I RGGNCCY 2 cut(s) 418, 751
EcoT14I CCWWGG 1 cut(s) 48
EcoT38I GRGCYC 2 cut(s) 30, 400
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 4 cut(s) 211, 349, 361, 382
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FatI CATG 4 cut(s) 207, 345, 357, 378
FauI CCCGC 2 cut(s) 143, 220
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 8 cut(s) 6, 54, 57, 60, 150, 153, 216, 566
FokI GGATG 1 cut(s) 47
FriOI GRGCYC 2 cut(s) 30, 400
Fsp4HI GCNGC 8 cut(s) 6, 54, 57, 60, 150, 153, 216, 566
GlaI GCGC 2 cut(s) 122, 554
GluI GCNGC 8 cut(s) 6, 54, 57, 60, 150, 153, 216, 566
GsaI CCCAGC 1 cut(s) 142
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 124
HaeIII GGCC 6 cut(s) 186, 392, 420, 493, 514, 720
HapII CCGG 4 cut(s) 99, 183, 366, 393
HhaI GCGC 2 cut(s) 123, 555
Hin1II CATG 4 cut(s) 211, 349, 361, 382
Hin6I GCGC 2 cut(s) 121, 553
HinP1I GCGC 2 cut(s) 121, 553
HincII GTYRAC 1 cut(s) 643
HindII GTYRAC 1 cut(s) 643
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 3 cut(s) 361, 528, 834
HpaII CCGG 4 cut(s) 99, 183, 366, 393
Hpy166II GTNNAC 4 cut(s) 96, 199, 230, 643
Hpy188I TCNGA 3 cut(s) 12, 118, 713
Hpy188III TCNNGA 2 cut(s) 358, 586
Hpy8I GTNNAC 4 cut(s) 96, 199, 230, 643
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 3 cut(s) 227, 329, 709
HpyCH4V TGCA 3 cut(s) 293, 349, 671
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 59, 545, 722
HpyF3I CTNAG 2 cut(s) 29, 587
Hsp92II CATG 4 cut(s) 211, 349, 361, 382
HspAI GCGC 2 cut(s) 121, 553
Kzo9I GATC 4 cut(s) 256, 274, 543, 733
LmnI GCTCC 4 cut(s) 13, 118, 395, 452
Lsp1109I GCAGC 2 cut(s) 139, 552
LweI GCATC 2 cut(s) 638, 704
MaeIII GTNAC 1 cut(s) 323
MalI GATC 4 cut(s) 258, 276, 545, 735
MboI GATC 4 cut(s) 256, 274, 543, 733
MboII GAAGA 8 cut(s) 50, 157, 160, 251, 334, 427, 450, 835
MflI RGATCY 1 cut(s) 733
MhlI GDGCHC 2 cut(s) 30, 400
MluCI AATT 3 cut(s) 311, 483, 521
MmeI TCCRAC 3 cut(s) 96, 623, 836
MnlI CCTC 6 cut(s) 58, 136, 140, 190, 582, 764
MseI TTAA 2 cut(s) 482, 765
MslI CAYNNNNRTG 1 cut(s) 344
MspA1I CMGCKG 3 cut(s) 138, 152, 449
MspI CCGG 4 cut(s) 99, 183, 366, 393
MspR9I CCNGG 1 cut(s) 366
Mva1269I GAATGC 1 cut(s) 49
MwoI GCNNNNNNNGC 4 cut(s) 53, 59, 545, 722
NciI CCSGG 1 cut(s) 366
NdeII GATC 4 cut(s) 256, 274, 543, 733
NlaIII CATG 4 cut(s) 211, 349, 361, 382
NlaIV GGNNCC 3 cut(s) 9, 286, 419
PagI TCATGA 1 cut(s) 357
PceI AGGCCT 1 cut(s) 493
PctI GAATGC 1 cut(s) 49
PfeI GAWTC 3 cut(s) 361, 528, 834
PfoI TCCNGGA 1 cut(s) 364
PkrI GCNGC 8 cut(s) 7, 55, 58, 61, 151, 154, 217, 567
PpuMI RGGWCCY 1 cut(s) 751
Psp124BI GAGCTC 1 cut(s) 400
Psp5II RGGWCCY 1 cut(s) 751
PspFI CCCAGC 1 cut(s) 138
PspN4I GGNNCC 3 cut(s) 9, 286, 419
PspPI GGNCC 4 cut(s) 93, 418, 513, 751
PspPPI RGGWCCY 1 cut(s) 751
PsuI RGATCY 1 cut(s) 733
PvuII CAGCTG 1 cut(s) 449
RseI CAYNNNNRTG 1 cut(s) 344
SacI GAGCTC 1 cut(s) 400
SaqAI TTAA 2 cut(s) 482, 765
SatI GCNGC 8 cut(s) 6, 54, 57, 60, 150, 153, 216, 566
Sau3AI GATC 4 cut(s) 256, 274, 543, 733
Sau96I GGNCC 4 cut(s) 93, 418, 513, 751
ScrFI CCNGG 1 cut(s) 366
SduI GDGCHC 2 cut(s) 30, 400
SfaNI GCATC 2 cut(s) 638, 704
SinI GGWCC 2 cut(s) 93, 751
SmiMI CAYNNNNRTG 1 cut(s) 344
SmlI CTYRAG 2 cut(s) 233, 737
SmoI CTYRAG 2 cut(s) 233, 737
Sse9I AATT 3 cut(s) 311, 483, 521
SseBI AGGCCT 1 cut(s) 493
SsiI CCGC 9 cut(s) 5, 53, 56, 59, 62, 136, 150, 213, 216
SstI GAGCTC 1 cut(s) 400
StuI AGGCCT 1 cut(s) 493
StyD4I CCNGG 1 cut(s) 364
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 3 cut(s) 227, 329, 709
TasI AATT 3 cut(s) 311, 483, 521
TauI GCSGC 6 cut(s) 8, 56, 59, 62, 152, 218
TfiI GAWTC 3 cut(s) 361, 528, 834
Tru1I TTAA 2 cut(s) 482, 765
Tru9I TTAA 2 cut(s) 482, 765
TscAI CASTG 1 cut(s) 804
TseI GCWGC 2 cut(s) 152, 565
TspDTI ATGAA 7 cut(s) 51, 160, 304, 334, 346, 367, 607
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 1 cut(s) 804
VpaK11BI GGWCC 2 cut(s) 93, 751
XmiI GTMKAC 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.