MD10G1048400.v1.1

membrane protein At4g09580-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008356
Physical Location & Seq
Reverse (-)
6408758 .. 6413482
4725 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1048400.v1.1.491

Sequence Viewer

Length: 831 bp
ATGGCGGCTCCGAGAAATCTGGCGGGGGACGCGGGGAGGCTGCCGAGGGACGAGGAGAATGCCAAGGAGGACGATTGGTCAACCGGCAAGAAGCTCAAGTCCGAAAGGTTTCCGCTTTCGCGGTGGGAGCTCGCGGCGGCTCTCGCCGTTGTCTTGGTCTTCTCTACCGGCCTCTCCTGTGTCTACTTGACCATGCCCGCCGCCGAGTACGGTAAACTCAAGCTGCCCCGCACCATTGTCGATCTTCGCCTTCTCAAAGACAATCTGGCAACATATGCCAATGATTATCCAGCACAATTTATTCTGGGTTACTGCTCTACTTACATCTTCATGCAGACTTTTATGATTCCTGGGACAATTTTTATGTCATTGCTAGCTGGAGCTCTTTTTGGTGTAATCAGGGGCCTTATCTTGGTTGTCTGCAACGCAACAGCTGGAGCGTCATCCTGCTTCTTTTTGTCTAAGTTAATTGGAAGGCCTTTTGTTAGTTGGTTTTGGCCAGAAAAGTTGAGATTTTTTCAGGCAGAGATTGCAAAGCGTAGGGAAAAGCTGCTAAATTACATGCTTTTTCTTAGGATAACTCCAACCTTGCCGAACCTATTCATCAACTTGGCATCTCCAATTGTTGATATACCGTTTCATATTTTCTTTCTGGCGACTTTTATTGGACTTATTCCAGCATCTTATATCACAGTCAGAGCTGGCCTTGCTCTGGGAGATCTCAATTCAGTCAAGGATCTCTATGACTTTAAAACTTTGTTTGTGCTATTCCTCATTGGCTCTTTGATTATATTTCCAACCGTTTTGAAGAGGAAGCGAATCTACGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

277

Amino Acids

31.06

Weight (kDa)

9.49

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 116 - 235 6.3e-16 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 183
AccII CGCG 3 cut(s) 32, 121, 134
AclWI GGATC 1 cut(s) 744
AcoI YGGCCR 1 cut(s) 497
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 2 cut(s) 412, 712
AgsI TTSAA 1 cut(s) 808
AhdI GACNNNNNGTC 1 cut(s) 76
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 8 cut(s) 94, 130, 223, 377, 383, 434, 550, 701
AluI AGCT 8 cut(s) 94, 130, 223, 377, 383, 434, 550, 701
Alw21I GWGCWC 2 cut(s) 132, 385
AlwI GGATC 1 cut(s) 744
AoxI GGCC 5 cut(s) 169, 403, 476, 497, 703
ApeKI GCWGC 3 cut(s) 40, 223, 550
Asp700I GAANNNNTTC 2 cut(s) 108, 599
AspS9I GGNCC 1 cut(s) 403
AsuNHI GCTAGC 1 cut(s) 373
BalI TGGCCA 1 cut(s) 499
BanII GRGCYC 2 cut(s) 132, 385
BarI GAAGNNNNNNTAC 1 cut(s) 806
BbsI GAAGAC 1 cut(s) 151
Bbv12I GWGCWC 2 cut(s) 132, 385
BbvI GCAGC 3 cut(s) 27, 210, 537
BceAI ACGGC 1 cut(s) 131
BcgI CGANNNNNNTGC 4 cut(s) 41, 75, 220, 254
BciT130I CCWGG 1 cut(s) 351
BfaI CTAG 1 cut(s) 374
BglII AGATCT 1 cut(s) 718
BisI GCNGC 7 cut(s) 6, 41, 135, 138, 201, 224, 551
BlsI GCNGC 7 cut(s) 7, 42, 136, 139, 202, 225, 552
Bme1390I CCNGG 1 cut(s) 351
BmeRI GACNNNNNGTC 1 cut(s) 76
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 2 cut(s) 9, 404
BmrFI CCNGG 1 cut(s) 351
BmsI GCATC 2 cut(s) 623, 689
BmtI GCTAGC 1 cut(s) 377
BpiI GAAGAC 1 cut(s) 151
BpmI CTGGAG 2 cut(s) 399, 456
BpuEI CTTGAG 2 cut(s) 80, 203
BsaJI CCNNGG 3 cut(s) 44, 63, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 412, 712
Bse118I RCCGGY 2 cut(s) 83, 167
Bse3DI GCAATG 1 cut(s) 368
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 3 cut(s) 44, 63, 350
BseGI GGATG 1 cut(s) 443
BseLI CCNNNNNNNGG 2 cut(s) 412, 712
BseMI GCAATG 1 cut(s) 368
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 3 cut(s) 27, 210, 537
Bsh1236I CGCG 3 cut(s) 32, 121, 134
BshFI GGCC 5 cut(s) 171, 405, 478, 499, 705
BsiHKAI GWGCWC 2 cut(s) 132, 385
BsiSI CCGG 2 cut(s) 84, 168
BslFI GGGAC 3 cut(s) 41, 62, 367
BslI CCNNNNNNNGG 2 cut(s) 412, 712
BsmFI GGGAC 3 cut(s) 41, 62, 367
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 5 cut(s) 171, 405, 478, 499, 705
Bsp1286I GDGCHC 2 cut(s) 132, 385
Bsp143I GATC 3 cut(s) 241, 718, 736
BspANI GGCC 5 cut(s) 171, 405, 478, 499, 705
BspFNI CGCG 3 cut(s) 32, 121, 134
BspLI GGNNCC 2 cut(s) 9, 404
BspOI GCTAGC 1 cut(s) 377
BspPI GGATC 1 cut(s) 744
BsrDI GCAATG 1 cut(s) 368
BsrFI RCCGGY 2 cut(s) 83, 167
BssAI RCCGGY 2 cut(s) 83, 167
BssECI CCNNGG 3 cut(s) 44, 63, 350
BssMI GATC 3 cut(s) 241, 718, 736
BssT1I CCWWGG 1 cut(s) 63
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 4 cut(s) 212, 636, 694, 802
Bst6I CTCTTC 1 cut(s) 803
BstAPI GCANNNNNTGC 2 cut(s) 275, 530
BstC8I GCNNGC 4 cut(s) 132, 198, 375, 703
BstDEI CTNAG 2 cut(s) 462, 572
BstF5I GGATG 1 cut(s) 443
BstFNI CGCG 3 cut(s) 32, 121, 134
BstKTI GATC 3 cut(s) 244, 721, 739
BstMBI GATC 3 cut(s) 241, 718, 736
BstMWI GCNNNNNNNGC 6 cut(s) 29, 127, 143, 275, 530, 707
BstNI CCWGG 1 cut(s) 351
BstNSI RCATGY 1 cut(s) 565
BstSCI CCNGG 1 cut(s) 349
BstUI CGCG 3 cut(s) 32, 121, 134
BstV1I GCAGC 3 cut(s) 27, 210, 537
BstV2I GAAGAC 1 cut(s) 151
BstX2I RGATCY 2 cut(s) 718, 736
BstYI RGATCY 2 cut(s) 718, 736
BsuRI GGCC 5 cut(s) 171, 405, 478, 499, 705
BtsCI GGATG 1 cut(s) 443
Cac8I GCNNGC 4 cut(s) 132, 198, 375, 703
Cfr10I RCCGGY 2 cut(s) 83, 167
Cfr13I GGNCC 1 cut(s) 403
CseI GACGC 2 cut(s) 38, 429
Csp6I GTAC 1 cut(s) 208
CviAII CATG 3 cut(s) 193, 331, 562
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 2 cut(s) 462, 572
DpnI GATC 3 cut(s) 243, 720, 738
DpnII GATC 3 cut(s) 241, 718, 736
DraI TTTAAA 1 cut(s) 751
DriI GACNNNNNGTC 1 cut(s) 76
EaeI YGGCCR 1 cut(s) 497
Eam1104I CTCTTC 1 cut(s) 803
Eam1105I GACNNNNNGTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 803
Ecl136II GAGCTC 2 cut(s) 130, 383
Eco130I CCWWGG 1 cut(s) 63
Eco147I AGGCCT 1 cut(s) 478
Eco24I GRGCYC 2 cut(s) 132, 385
Eco53kI GAGCTC 2 cut(s) 130, 383
EcoICRI GAGCTC 2 cut(s) 130, 383
EcoO109I RGGNCCY 1 cut(s) 403
EcoRII CCWGG 1 cut(s) 349
EcoT14I CCWWGG 1 cut(s) 63
EcoT38I GRGCYC 2 cut(s) 132, 385
ErhI CCWWGG 1 cut(s) 63
FaeI CATG 3 cut(s) 196, 334, 565
FaqI GGGAC 3 cut(s) 41, 62, 367
FatI CATG 3 cut(s) 192, 330, 561
FauI CCCGC 4 cut(s) 16, 25, 205, 236
FauNDI CATATG 1 cut(s) 274
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 7 cut(s) 6, 41, 135, 138, 201, 224, 551
FokI GGATG 1 cut(s) 430
FriOI GRGCYC 2 cut(s) 132, 385
Fsp4HI GCNGC 7 cut(s) 6, 41, 135, 138, 201, 224, 551
FspBI CTAG 1 cut(s) 374
GluI GCNGC 7 cut(s) 6, 41, 135, 138, 201, 224, 551
GsuI CTGGAG 2 cut(s) 399, 456
HaeIII GGCC 5 cut(s) 171, 405, 478, 499, 705
HapII CCGG 2 cut(s) 84, 168
HgaI GACGC 2 cut(s) 38, 429
Hin1II CATG 3 cut(s) 196, 334, 565
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HinfI GANTC 2 cut(s) 346, 819
HpaII CCGG 2 cut(s) 84, 168
Hpy166II GTNNAC 3 cut(s) 81, 184, 215
Hpy188I TCNGA 3 cut(s) 12, 103, 698
Hpy8I GTNNAC 3 cut(s) 81, 184, 215
HpyAV CCTTC 2 cut(s) 260, 468
HpyCH4III ACNGT 4 cut(s) 212, 636, 694, 802
HpyCH4V TGCA 3 cut(s) 334, 423, 533
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 127, 143, 275, 530, 707
HpyF3I CTNAG 2 cut(s) 462, 572
Hsp92II CATG 3 cut(s) 196, 334, 565
Kzo9I GATC 3 cut(s) 241, 718, 736
LmnI GCTCC 4 cut(s) 13, 127, 380, 437
Lsp1109I GCAGC 3 cut(s) 27, 210, 537
LweI GCATC 2 cut(s) 623, 689
MaeI CTAG 1 cut(s) 374
MaeIII GTNAC 1 cut(s) 308
MalI GATC 3 cut(s) 243, 720, 738
MboI GATC 3 cut(s) 241, 718, 736
MboII GAAGA 4 cut(s) 151, 236, 319, 820
MfeI CAATTG 1 cut(s) 621
MflI RGATCY 2 cut(s) 718, 736
MhlI GDGCHC 2 cut(s) 132, 385
MlsI TGGCCA 1 cut(s) 499
MluCI AATT 6 cut(s) 296, 357, 468, 556, 621, 724
MluNI TGGCCA 1 cut(s) 499
MmeI TCCRAC 2 cut(s) 608, 821
MnlI CCTC 7 cut(s) 30, 39, 46, 61, 182, 782, 804
Mox20I TGGCCA 1 cut(s) 499
MroXI GAANNNNTTC 2 cut(s) 108, 599
MscI TGGCCA 1 cut(s) 499
MseI TTAA 2 cut(s) 467, 750
MslI CAYNNNNRTG 1 cut(s) 329
Msp20I TGGCCA 1 cut(s) 499
MspA1I CMGCKG 1 cut(s) 434
MspI CCGG 2 cut(s) 84, 168
MspR9I CCNGG 1 cut(s) 351
MunI CAATTG 1 cut(s) 621
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 1 cut(s) 351
MvnI CGCG 3 cut(s) 32, 121, 134
MwoI GCNNNNNNNGC 6 cut(s) 29, 127, 143, 275, 530, 707
NdeI CATATG 1 cut(s) 274
NdeII GATC 3 cut(s) 241, 718, 736
NheI GCTAGC 1 cut(s) 373
NlaIII CATG 3 cut(s) 196, 334, 565
NlaIV GGNNCC 2 cut(s) 9, 404
NmeAIII GCCGAG 2 cut(s) 69, 229
NspI RCATGY 1 cut(s) 565
PceI AGGCCT 1 cut(s) 478
PctI GAATGC 1 cut(s) 64
PdmI GAANNNNTTC 2 cut(s) 108, 599
PfeI GAWTC 2 cut(s) 346, 819
PkrI GCNGC 7 cut(s) 7, 42, 136, 139, 202, 225, 552
Psp124BI GAGCTC 2 cut(s) 132, 385
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 2 cut(s) 9, 404
PspPI GGNCC 1 cut(s) 403
PsuI RGATCY 2 cut(s) 718, 736
PvuII CAGCTG 1 cut(s) 434
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 329
SacI GAGCTC 2 cut(s) 132, 385
SaqAI TTAA 2 cut(s) 467, 750
SatI GCNGC 7 cut(s) 6, 41, 135, 138, 201, 224, 551
Sau3AI GATC 3 cut(s) 241, 718, 736
Sau96I GGNCC 1 cut(s) 403
ScrFI CCNGG 1 cut(s) 351
SduI GDGCHC 2 cut(s) 132, 385
SfaNI GCATC 2 cut(s) 623, 689
SmiMI CAYNNNNRTG 1 cut(s) 329
SmlI CTYRAG 2 cut(s) 95, 218
SmoI CTYRAG 2 cut(s) 95, 218
Sse9I AATT 6 cut(s) 296, 357, 468, 556, 621, 724
SseBI AGGCCT 1 cut(s) 478
SspMI CTAG 1 cut(s) 374
SstI GAGCTC 2 cut(s) 132, 385
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 1 cut(s) 349
StyI CCWWGG 1 cut(s) 63
TaaI ACNGT 4 cut(s) 212, 636, 694, 802
TaqI TCGA 1 cut(s) 240
TasI AATT 6 cut(s) 296, 357, 468, 556, 621, 724
TauI GCSGC 4 cut(s) 8, 137, 140, 203
TfiI GAWTC 2 cut(s) 346, 819
Tru1I TTAA 2 cut(s) 467, 750
Tru9I TTAA 2 cut(s) 467, 750
TseI GCWGC 3 cut(s) 40, 223, 550
TspDTI ATGAA 3 cut(s) 319, 592, 629
XceI RCATGY 1 cut(s) 565
XmiI GTMKAC 1 cut(s) 183
XmnI GAANNNNTTC 2 cut(s) 108, 599
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.