FvH4_2g16730

EC protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009194
Physical Location & Seq
Reverse (-)
14578849 .. 14580071
1223 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g16730.t1

Sequence Viewer

Length: 258 bp
ATGGCAGAAACAGTTGGAGATGTGAAGATGTCCTGCAACGATACCTGTGGCTGCACTGTTCCGTGCCCCGGCGGCGTGTCCTGCAGGTGCACATCCAAGACCGAGGCCACAAGTGGGGATGGCCACCTGAAGTGCTCGTGCGGCGACCACTGCGGCTGCAACCCGTGCACTTGTGCTAAGAGCGTCGTCTCCACCGGGGTAGGCAAGGCTTACTGCAAGTGCGGCACTGGTTGTACCTGTGTCACATGCTCCGCCTAA

Protein Analysis

86

Amino Acids

8.39

Weight (kDa)

7.35

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_PEC PF02068 12 - 84 2.1e-24 Plant PEC family metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 75
Acc36I ACCTGC 1 cut(s) 75
AciI CCGC 5 cut(s) 72, 141, 153, 222, 252
AcoI YGGCCR 1 cut(s) 121
AcuI CTGAAG 1 cut(s) 149
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 2 cut(s) 68, 114
Alw21I GWGCWC 3 cut(s) 92, 137, 170
Alw26I GTCTC 1 cut(s) 193
Alw44I GTGCAC 2 cut(s) 88, 166
AoxI GGCC 2 cut(s) 105, 121
ApaLI GTGCAC 2 cut(s) 88, 166
ApeKI GCWGC 2 cut(s) 51, 156
AsuC2I CCSGG 2 cut(s) 69, 196
BaeGI GKGCMC 3 cut(s) 68, 92, 170
BalI TGGCCA 1 cut(s) 123
BauI CACGAG 1 cut(s) 136
Bbv12I GWGCWC 3 cut(s) 92, 137, 170
BbvI GCAGC 2 cut(s) 38, 143
BccI CCATC 1 cut(s) 113
BcnI CCSGG 2 cut(s) 69, 196
BcoDI GTCTC 1 cut(s) 193
BfmI CTRYAG 1 cut(s) 82
BfuAI ACCTGC 1 cut(s) 75
BglI GCCNNNNNGGC 1 cut(s) 72
BisI GCNGC 6 cut(s) 52, 73, 142, 154, 157, 223
BlsI GCNGC 6 cut(s) 53, 74, 143, 155, 158, 224
Bme1390I CCNGG 2 cut(s) 69, 196
BmrFI CCNGG 2 cut(s) 69, 196
BpuMI CCSGG 2 cut(s) 69, 196
BsaJI CCNNGG 3 cut(s) 67, 102, 195
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 114
Bse1I ACTGG 1 cut(s) 232
BseDI CCNNGG 3 cut(s) 67, 102, 195
BseGI GGATG 2 cut(s) 92, 124
BseLI CCNNNNNNNGG 2 cut(s) 68, 114
BseNI ACTGG 1 cut(s) 232
BseSI GKGCMC 3 cut(s) 68, 92, 170
BseXI GCAGC 2 cut(s) 38, 143
BsgI GTGCAG 1 cut(s) 37
BshFI GGCC 2 cut(s) 107, 123
BsiHKAI GWGCWC 3 cut(s) 92, 137, 170
BsiSI CCGG 2 cut(s) 69, 195
BslI CCNNNNNNNGG 2 cut(s) 68, 114
BsmAI GTCTC 1 cut(s) 193
BsmBI CGTCTC 1 cut(s) 193
BsnI GGCC 2 cut(s) 107, 123
Bsp1286I GDGCHC 4 cut(s) 68, 92, 137, 170
BspACI CCGC 5 cut(s) 72, 141, 153, 222, 252
BspANI GGCC 2 cut(s) 107, 123
BspMAI CTGCAG 1 cut(s) 86
BspMI ACCTGC 1 cut(s) 75
BsrI ACTGG 1 cut(s) 232
BssECI CCNNGG 3 cut(s) 67, 102, 195
BssSI CACGAG 1 cut(s) 136
Bst2BI CACGAG 1 cut(s) 136
Bst4CI ACNGT 2 cut(s) 13, 58
BstAPI GCANNNNNTGC 1 cut(s) 165
BstDEI CTNAG 1 cut(s) 177
BstF5I GGATG 2 cut(s) 92, 124
BstMAI GTCTC 1 cut(s) 193
BstMWI GCNNNNNNNGC 6 cut(s) 72, 81, 141, 150, 165, 222
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 2 cut(s) 67, 194
BstSFI CTRYAG 1 cut(s) 82
BstSLI GKGCMC 3 cut(s) 68, 92, 170
BstV1I GCAGC 2 cut(s) 38, 143
BsuRI GGCC 2 cut(s) 107, 123
BtsCI GGATG 2 cut(s) 92, 124
BtsI GCAGTG 1 cut(s) 148
BtsIMutI CAGTG 3 cut(s) 54, 148, 225
BveI ACCTGC 1 cut(s) 75
CseI GACGC 1 cut(s) 172
Csp6I GTAC 1 cut(s) 234
CviAII CATG 1 cut(s) 246
CviJI RGCY 5 cut(s) 51, 107, 123, 156, 209
CviKI_1 RGCY 5 cut(s) 51, 107, 123, 156, 209
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 1 cut(s) 177
EaeI YGGCCR 1 cut(s) 121
EciI GGCGGA 1 cut(s) 241
Eco57I CTGAAG 1 cut(s) 149
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 1 cut(s) 249
FaiI YATR 1 cut(s) 247
FatI CATG 1 cut(s) 245
Fnu4HI GCNGC 6 cut(s) 52, 73, 142, 154, 157, 223
FokI GGATG 2 cut(s) 79, 131
Fsp4HI GCNGC 6 cut(s) 52, 73, 142, 154, 157, 223
GluI GCNGC 6 cut(s) 52, 73, 142, 154, 157, 223
HaeIII GGCC 2 cut(s) 107, 123
HapII CCGG 2 cut(s) 69, 195
HgaI GACGC 1 cut(s) 172
Hin1II CATG 1 cut(s) 249
HpaII CCGG 2 cut(s) 69, 195
Hpy166II GTNNAC 2 cut(s) 90, 168
Hpy8I GTNNAC 2 cut(s) 90, 168
Hpy99I CGWCG 1 cut(s) 188
HpyCH4III ACNGT 2 cut(s) 13, 58
HpyCH4V TGCA 7 cut(s) 36, 54, 84, 90, 159, 168, 216
HpyF10VI GCNNNNNNNGC 6 cut(s) 72, 81, 141, 150, 165, 222
HpyF3I CTNAG 1 cut(s) 177
Hsp92II CATG 1 cut(s) 249
LmnI GCTCC 1 cut(s) 254
LpnPI CCDG 9 cut(s) 46, 58, 70, 82, 94, 140, 208, 213, 250
Lsp1109I GCAGC 2 cut(s) 38, 143
MaeIII GTNAC 1 cut(s) 241
MboII GAAGA 1 cut(s) 37
MhlI GDGCHC 4 cut(s) 68, 92, 137, 170
MlsI TGGCCA 1 cut(s) 123
MluNI TGGCCA 1 cut(s) 123
MnlI CCTC 1 cut(s) 97
Mox20I TGGCCA 1 cut(s) 123
MscI TGGCCA 1 cut(s) 123
Msp20I TGGCCA 1 cut(s) 123
MspI CCGG 2 cut(s) 69, 195
MspR9I CCNGG 2 cut(s) 69, 196
MwoI GCNNNNNNNGC 6 cut(s) 72, 81, 141, 150, 165, 222
NciI CCSGG 2 cut(s) 69, 196
NlaIII CATG 1 cut(s) 249
NmuCI GTSAC 1 cut(s) 241
NspI RCATGY 1 cut(s) 249
PaqCI CACCTGC 1 cut(s) 75
PkrI GCNGC 6 cut(s) 53, 74, 143, 155, 158, 224
PstI CTGCAG 1 cut(s) 86
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SatI GCNGC 6 cut(s) 52, 73, 142, 154, 157, 223
SbfI CCTGCAGG 1 cut(s) 86
ScrFI CCNGG 2 cut(s) 69, 196
SdaI CCTGCAGG 1 cut(s) 86
SduI GDGCHC 4 cut(s) 68, 92, 137, 170
SetI ASST 4 cut(s) 47, 89, 129, 239
SfcI CTRYAG 1 cut(s) 82
Sse8387I CCTGCAGG 1 cut(s) 86
SsiI CCGC 5 cut(s) 72, 141, 153, 222, 252
StyD4I CCNGG 2 cut(s) 67, 194
TaaI ACNGT 2 cut(s) 13, 58
TaqII GACCGA 1 cut(s) 116
TauI GCSGC 4 cut(s) 75, 144, 156, 225
TscAI CASTG 3 cut(s) 61, 155, 232
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 2 cut(s) 51, 156
Tsp45I GTSAC 1 cut(s) 241
TspGWI ACGGA 1 cut(s) 51
TspRI CASTG 3 cut(s) 61, 155, 232
VneI GTGCAC 2 cut(s) 88, 166
XceI RCATGY 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.