Rroxscaffold_7G00187560

EC protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009194
Physical Location & Seq
Forward (+)
26972905 .. 26973581
677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00187560.1

Sequence Viewer

Length: 258 bp
ATGGCAGATACAGTTGGAGGTGTGCAGATGTCCTGCAACGATAGCTGTGGCTGCCCTGTTCCGTGCCCTGGCGGCGTGTCCTGTAGGTGCACAACCAGGACGGAGGCCACGAGTGGGGACGGCCACATGAAGTGCTCGTGCGGCGAGCACTGTGGCTGCAACCCGTGCACTTGTGCTAAGAGCGTCGTCTCTACCAGAGTAGGCAGGGCCTACTGCAAGTGCGGCGAGGGTTGTACCTGTGTCTCATGCTCTGCCTAA

Protein Analysis

85

Amino Acids

8.51

Weight (kDa)

7.36

Isoelectric Point (pI)

53.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_PEC PF02068 12 - 84 4e-25 Plant PEC family metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 72, 141, 222
AcoI YGGCCR 1 cut(s) 121
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 2 cut(s) 68, 114
AjnI CCWGG 2 cut(s) 67, 95
AluBI AGCT 1 cut(s) 45
AluI AGCT 1 cut(s) 45
Alw21I GWGCWC 4 cut(s) 92, 137, 150, 170
Alw26I GTCTC 2 cut(s) 193, 247
Alw44I GTGCAC 2 cut(s) 88, 166
AoxI GGCC 3 cut(s) 105, 121, 207
ApaLI GTGCAC 2 cut(s) 88, 166
ApeKI GCWGC 2 cut(s) 51, 156
AspS9I GGNCC 1 cut(s) 207
BaeGI GKGCMC 3 cut(s) 68, 92, 170
BauI CACGAG 2 cut(s) 109, 136
Bbv12I GWGCWC 4 cut(s) 92, 137, 150, 170
BbvI GCAGC 2 cut(s) 38, 143
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 2 cut(s) 69, 97
BcoDI GTCTC 2 cut(s) 193, 247
BfmI CTRYAG 1 cut(s) 82
BglI GCCNNNNNGGC 1 cut(s) 72
BisI GCNGC 5 cut(s) 52, 73, 142, 157, 223
BlsI GCNGC 5 cut(s) 53, 74, 143, 158, 224
Bme1390I CCNGG 2 cut(s) 69, 97
BmgT120I GGNCC 1 cut(s) 207
BmrFI CCNGG 2 cut(s) 69, 97
BsaJI CCNNGG 1 cut(s) 67
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 114
BseBI CCWGG 2 cut(s) 69, 97
BseDI CCNNGG 1 cut(s) 67
BseLI CCNNNNNNNGG 2 cut(s) 68, 114
BseSI GKGCMC 3 cut(s) 68, 92, 170
BseXI GCAGC 2 cut(s) 38, 143
BsgI GTGCAG 1 cut(s) 44
BshFI GGCC 3 cut(s) 107, 123, 209
BsiHKAI GWGCWC 4 cut(s) 92, 137, 150, 170
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 68, 114
BsmAI GTCTC 2 cut(s) 193, 247
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 3 cut(s) 107, 123, 209
Bsp1286I GDGCHC 5 cut(s) 68, 92, 137, 150, 170
BspACI CCGC 3 cut(s) 72, 141, 222
BspANI GGCC 3 cut(s) 107, 123, 209
BssECI CCNNGG 1 cut(s) 67
BssSI CACGAG 2 cut(s) 109, 136
Bst2BI CACGAG 2 cut(s) 109, 136
Bst2UI CCWGG 2 cut(s) 69, 97
Bst4CI ACNGT 2 cut(s) 13, 152
BstAPI GCANNNNNTGC 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 146
BstDEI CTNAG 1 cut(s) 177
BstMAI GTCTC 2 cut(s) 193, 247
BstMWI GCNNNNNNNGC 6 cut(s) 42, 51, 72, 141, 165, 222
BstNI CCWGG 2 cut(s) 69, 97
BstSCI CCNGG 2 cut(s) 67, 95
BstSFI CTRYAG 1 cut(s) 82
BstSLI GKGCMC 3 cut(s) 68, 92, 170
BstV1I GCAGC 2 cut(s) 38, 143
BsuRI GGCC 3 cut(s) 107, 123, 209
BtsIMutI CAGTG 1 cut(s) 148
Cac8I GCNNGC 1 cut(s) 146
Cfr13I GGNCC 1 cut(s) 207
CseI GACGC 1 cut(s) 172
Csp6I GTAC 1 cut(s) 234
CviAII CATG 2 cut(s) 127, 246
CviJI RGCY 6 cut(s) 45, 51, 107, 123, 156, 209
CviKI_1 RGCY 6 cut(s) 45, 51, 107, 123, 156, 209
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 1 cut(s) 177
EaeI YGGCCR 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 207
EcoRII CCWGG 2 cut(s) 67, 95
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 2 cut(s) 130, 249
FaiI YATR 2 cut(s) 128, 247
FaqI GGGAC 1 cut(s) 131
FatI CATG 2 cut(s) 126, 245
Fnu4HI GCNGC 5 cut(s) 52, 73, 142, 157, 223
Fsp4HI GCNGC 5 cut(s) 52, 73, 142, 157, 223
GluI GCNGC 5 cut(s) 52, 73, 142, 157, 223
HaeIII GGCC 3 cut(s) 107, 123, 209
HgaI GACGC 1 cut(s) 172
Hin1II CATG 2 cut(s) 130, 249
Hpy166II GTNNAC 2 cut(s) 90, 168
Hpy8I GTNNAC 2 cut(s) 90, 168
Hpy99I CGWCG 1 cut(s) 188
HpyCH4III ACNGT 2 cut(s) 13, 152
HpyCH4V TGCA 6 cut(s) 25, 36, 90, 159, 168, 216
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 51, 72, 141, 165, 222
HpyF3I CTNAG 1 cut(s) 177
Hsp92II CATG 2 cut(s) 130, 249
Lsp1109I GCAGC 2 cut(s) 38, 143
MhlI GDGCHC 5 cut(s) 68, 92, 137, 150, 170
MnlI CCTC 3 cut(s) 11, 97, 220
MspR9I CCNGG 2 cut(s) 69, 97
MvaI CCWGG 2 cut(s) 69, 97
MwoI GCNNNNNNNGC 6 cut(s) 42, 51, 72, 141, 165, 222
NlaIII CATG 2 cut(s) 130, 249
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PkrI GCNGC 5 cut(s) 53, 74, 143, 158, 224
Psp6I CCWGG 2 cut(s) 67, 95
PspGI CCWGG 2 cut(s) 67, 95
PspPI GGNCC 1 cut(s) 207
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SatI GCNGC 5 cut(s) 52, 73, 142, 157, 223
Sau96I GGNCC 1 cut(s) 207
ScrFI CCNGG 2 cut(s) 69, 97
SduI GDGCHC 5 cut(s) 68, 92, 137, 150, 170
SetI ASST 4 cut(s) 22, 47, 89, 239
SfcI CTRYAG 1 cut(s) 82
SsiI CCGC 3 cut(s) 72, 141, 222
StyD4I CCNGG 2 cut(s) 67, 95
TaaI ACNGT 2 cut(s) 13, 152
TauI GCSGC 3 cut(s) 75, 144, 225
TscAI CASTG 1 cut(s) 155
TseI GCWGC 2 cut(s) 51, 156
TspDTI ATGAA 1 cut(s) 143
TspGWI ACGGA 2 cut(s) 51, 116
TspRI CASTG 1 cut(s) 155
VneI GTGCAC 2 cut(s) 88, 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.