Rh6CG251200

EC protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0009194
Physical Location & Seq
Reverse (-)
42152616 .. 42154097
1482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG251200.1

Sequence Viewer

Length: 234 bp
ATGGCAGATACAGTTGGAGGTGTGAAGATGTCCTGCAACGATAGCTGTGGCTGCCCTGTTCCGTGCCCTGGCGGCGTGTCTTGTAGGTGCAGAACCAGGACGGAGGCCACGAGTGGGGACGGCCACATGAAGTGCTCGTGCGGCGAGCACTGTGGCTGCAACCCGTGCGCTTGTGCTAAGAGCGTCGTCTCTACCGGAGTAGGCAGGGCCTACTGCAGAGAAGTCGATCAGTAG

Protein Analysis

77

Amino Acids

7.84

Weight (kDa)

7.42

Isoelectric Point (pI)

49.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_PEC PF02068 12 - 73 4.5e-21 Plant PEC family metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 72, 141
AcoI YGGCCR 1 cut(s) 121
AfiI CCNNNNNNNGG 2 cut(s) 68, 114
AjnI CCWGG 2 cut(s) 67, 95
AluBI AGCT 1 cut(s) 45
AluI AGCT 1 cut(s) 45
Alw21I GWGCWC 2 cut(s) 137, 150
Alw26I GTCTC 1 cut(s) 193
AoxI GGCC 3 cut(s) 105, 121, 207
ApeKI GCWGC 2 cut(s) 51, 156
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 1 cut(s) 207
BaeGI GKGCMC 1 cut(s) 68
BauI CACGAG 2 cut(s) 109, 136
Bbv12I GWGCWC 2 cut(s) 137, 150
BbvI GCAGC 2 cut(s) 38, 143
BceAI ACGGC 1 cut(s) 136
BcgI CGANNNNNNTGC 1 cut(s) 205
BciT130I CCWGG 2 cut(s) 69, 97
BcoDI GTCTC 1 cut(s) 193
BfmI CTRYAG 1 cut(s) 214
BglI GCCNNNNNGGC 1 cut(s) 72
BisI GCNGC 4 cut(s) 52, 73, 142, 157
BlsI GCNGC 4 cut(s) 53, 74, 143, 158
Bme1390I CCNGG 2 cut(s) 69, 97
BmgT120I GGNCC 1 cut(s) 207
BmrFI CCNGG 2 cut(s) 69, 97
BsaJI CCNNGG 1 cut(s) 67
BsaWI WCCGGW 1 cut(s) 194
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 114
BseBI CCWGG 2 cut(s) 69, 97
BseDI CCNNGG 1 cut(s) 67
BseLI CCNNNNNNNGG 2 cut(s) 68, 114
BseSI GKGCMC 1 cut(s) 68
BseXI GCAGC 2 cut(s) 38, 143
BsgI GTGCAG 1 cut(s) 109
BshFI GGCC 3 cut(s) 107, 123, 209
BsiHKAI GWGCWC 2 cut(s) 137, 150
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 68, 114
BsmAI GTCTC 1 cut(s) 193
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 3 cut(s) 107, 123, 209
Bsp1286I GDGCHC 3 cut(s) 68, 137, 150
Bsp143I GATC 1 cut(s) 226
BspACI CCGC 2 cut(s) 72, 141
BspANI GGCC 3 cut(s) 107, 123, 209
BspMAI CTGCAG 1 cut(s) 218
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 1 cut(s) 226
BssSI CACGAG 2 cut(s) 109, 136
Bst2BI CACGAG 2 cut(s) 109, 136
Bst2UI CCWGG 2 cut(s) 69, 97
Bst4CI ACNGT 2 cut(s) 13, 152
BstAPI GCANNNNNTGC 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 146
BstDEI CTNAG 1 cut(s) 177
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 1 cut(s) 229
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 1 cut(s) 226
BstMWI GCNNNNNNNGC 5 cut(s) 42, 51, 72, 141, 165
BstNI CCWGG 2 cut(s) 69, 97
BstSCI CCNGG 2 cut(s) 67, 95
BstSFI CTRYAG 1 cut(s) 214
BstSLI GKGCMC 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 38, 143
BsuRI GGCC 3 cut(s) 107, 123, 209
BtsIMutI CAGTG 1 cut(s) 148
Cac8I GCNNGC 1 cut(s) 146
CfoI GCGC 1 cut(s) 170
Cfr13I GGNCC 1 cut(s) 207
CseI GACGC 1 cut(s) 172
CviAII CATG 1 cut(s) 127
CviJI RGCY 6 cut(s) 45, 51, 107, 123, 156, 209
CviKI_1 RGCY 6 cut(s) 45, 51, 107, 123, 156, 209
DdeI CTNAG 1 cut(s) 177
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 207
EcoRII CCWGG 2 cut(s) 67, 95
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 1 cut(s) 130
FaiI YATR 1 cut(s) 128
FaqI GGGAC 1 cut(s) 131
FatI CATG 1 cut(s) 126
Fnu4HI GCNGC 4 cut(s) 52, 73, 142, 157
Fsp4HI GCNGC 4 cut(s) 52, 73, 142, 157
GlaI GCGC 1 cut(s) 169
GluI GCNGC 4 cut(s) 52, 73, 142, 157
HaeIII GGCC 3 cut(s) 107, 123, 209
HapII CCGG 1 cut(s) 195
HgaI GACGC 1 cut(s) 172
HhaI GCGC 1 cut(s) 170
Hin1II CATG 1 cut(s) 130
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HpaII CCGG 1 cut(s) 195
Hpy99I CGWCG 1 cut(s) 188
HpyCH4III ACNGT 2 cut(s) 13, 152
HpyCH4V TGCA 4 cut(s) 36, 90, 159, 216
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 51, 72, 141, 165
HpyF3I CTNAG 1 cut(s) 177
Hsp92II CATG 1 cut(s) 130
HspAI GCGC 1 cut(s) 168
Kzo9I GATC 1 cut(s) 226
LpnPI CCDG 8 cut(s) 46, 54, 69, 81, 82, 109, 190, 208
Lsp1109I GCAGC 2 cut(s) 38, 143
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MboII GAAGA 1 cut(s) 37
MhlI GDGCHC 3 cut(s) 68, 137, 150
MnlI CCTC 2 cut(s) 11, 97
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 2 cut(s) 69, 97
MvaI CCWGG 2 cut(s) 69, 97
MwoI GCNNNNNNNGC 5 cut(s) 42, 51, 72, 141, 165
NdeII GATC 1 cut(s) 226
NlaIII CATG 1 cut(s) 130
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PkrI GCNGC 4 cut(s) 53, 74, 143, 158
Psp6I CCWGG 2 cut(s) 67, 95
PspGI CCWGG 2 cut(s) 67, 95
PspPI GGNCC 1 cut(s) 207
PstI CTGCAG 1 cut(s) 218
SatI GCNGC 4 cut(s) 52, 73, 142, 157
Sau3AI GATC 1 cut(s) 226
Sau96I GGNCC 1 cut(s) 207
ScrFI CCNGG 2 cut(s) 69, 97
SduI GDGCHC 3 cut(s) 68, 137, 150
SetI ASST 3 cut(s) 22, 47, 89
SfcI CTRYAG 1 cut(s) 214
SsiI CCGC 2 cut(s) 72, 141
StyD4I CCNGG 2 cut(s) 67, 95
TaaI ACNGT 2 cut(s) 13, 152
TaqI TCGA 1 cut(s) 225
TauI GCSGC 2 cut(s) 75, 144
TscAI CASTG 1 cut(s) 155
TseI GCWGC 2 cut(s) 51, 156
TspDTI ATGAA 1 cut(s) 143
TspGWI ACGGA 2 cut(s) 51, 116
TspRI CASTG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.