MD05G1135300.v1.1

EC protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009194
Physical Location & Seq
Reverse (-)
26346834 .. 26347583
750 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1135300.v1.1.491

Sequence Viewer

Length: 258 bp
ATGGCAGATACAACCACAGCGGGGGGTCTCAGGGCCAGCTGCAACGACCGATGCGGCTGCACTAATCCGTGCCCCGGTGGCGCCTCTTGCAAGTGCACAACCACCTCCGACACCACAAGCGGGGGCGACCACATGACATGCTCCTGTGGCGAGCACTGCAGCTGCAACCCATGCACTTGCGCTGAGAGCGTCGTCACGGCTGGCAAGGCCAAATGCACGTGTGGCGCGGGCTGCGCATGCGTCTCTTGCGCAGCCTAA

Protein Analysis

86

Amino Acids

8.19

Weight (kDa)

5.98

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_PEC PF02068 14 - 84 1.5e-21 Plant PEC family metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 235, 250
AccB1I GGYRCC 1 cut(s) 80
AccII CGCG 1 cut(s) 227
AciI CCGC 4 cut(s) 20, 54, 120, 227
AcvI CACGTG 1 cut(s) 219
AcyI GRCGYC 1 cut(s) 81
AfiI CCNNNNNNNGG 3 cut(s) 21, 74, 120
AflIII ACRYGT 1 cut(s) 218
AluBI AGCT 2 cut(s) 39, 162
AluI AGCT 2 cut(s) 39, 162
Alw21I GWGCWC 2 cut(s) 98, 156
Alw26I GTCTC 2 cut(s) 32, 247
Alw44I GTGCAC 1 cut(s) 94
AoxI GGCC 2 cut(s) 33, 207
ApaLI GTGCAC 1 cut(s) 94
ApeKI GCWGC 6 cut(s) 39, 57, 159, 162, 231, 251
AspLEI GCGC 5 cut(s) 83, 182, 227, 236, 251
AspS9I GGNCC 1 cut(s) 33
AsuC2I CCSGG 1 cut(s) 75
BaeGI GKGCMC 2 cut(s) 74, 98
BanI GGYRCC 1 cut(s) 80
BbrPI CACGTG 1 cut(s) 219
Bbv12I GWGCWC 2 cut(s) 98, 156
BbvI GCAGC 5 cut(s) 26, 44, 149, 171, 218
BceAI ACGGC 1 cut(s) 213
BcgI CGANNNNNNTGC 2 cut(s) 39, 73
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 2 cut(s) 32, 247
BfmI CTRYAG 1 cut(s) 157
BfoI RGCGCY 1 cut(s) 84
BglI GCCNNNNNGGC 1 cut(s) 78
BisI GCNGC 7 cut(s) 40, 55, 58, 160, 163, 232, 252
BlsI GCNGC 7 cut(s) 41, 56, 59, 161, 164, 233, 253
Bme1390I CCNGG 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 33
BmiI GGNNCC 1 cut(s) 82
BmrFI CCNGG 1 cut(s) 75
BmsI GCATC 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 75
BsaAI YACGTR 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 73
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 74, 120
BseDI CCNNGG 1 cut(s) 73
BseLI CCNNNNNNNGG 3 cut(s) 21, 74, 120
BseMII CTCAG 2 cut(s) 43, 174
BseSI GKGCMC 2 cut(s) 74, 98
BseXI GCAGC 5 cut(s) 26, 44, 149, 171, 218
BsgI GTGCAG 1 cut(s) 43
Bsh1236I CGCG 1 cut(s) 227
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 2 cut(s) 35, 209
BshNI GGYRCC 1 cut(s) 80
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 2 cut(s) 98, 156
BsiSI CCGG 1 cut(s) 75
BslI CCNNNNNNNGG 3 cut(s) 21, 74, 120
BsmAI GTCTC 2 cut(s) 32, 247
BsmBI CGTCTC 1 cut(s) 247
BsnI GGCC 2 cut(s) 35, 209
Bso31I GGTCTC 1 cut(s) 32
Bsp1286I GDGCHC 3 cut(s) 74, 98, 156
BspACI CCGC 4 cut(s) 20, 54, 120, 227
BspANI GGCC 2 cut(s) 35, 209
BspCNI CTCAG 2 cut(s) 42, 175
BspFNI CGCG 1 cut(s) 227
BspLI GGNNCC 1 cut(s) 82
BspMAI CTGCAG 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 80
BspTNI GGTCTC 1 cut(s) 32
BssECI CCNNGG 1 cut(s) 73
BssNI GRCGYC 1 cut(s) 81
BstACI GRCGYC 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 171
BstBAI YACGTR 1 cut(s) 219
BstC8I GCNNGC 5 cut(s) 37, 152, 202, 229, 238
BstDEI CTNAG 2 cut(s) 29, 183
BstFNI CGCG 1 cut(s) 227
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 5 cut(s) 83, 182, 227, 236, 251
BstMAI GTCTC 2 cut(s) 32, 247
BstMCI CGRYCG 1 cut(s) 49
BstNSI RCATGY 2 cut(s) 141, 240
BstSCI CCNGG 1 cut(s) 73
BstSFI CTRYAG 1 cut(s) 157
BstSLI GKGCMC 2 cut(s) 74, 98
BstUI CGCG 1 cut(s) 227
BstV1I GCAGC 5 cut(s) 26, 44, 149, 171, 218
BsuRI GGCC 2 cut(s) 35, 209
BtsI GCAGTG 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 154
Cac8I GCNNGC 5 cut(s) 37, 152, 202, 229, 238
CfoI GCGC 5 cut(s) 83, 182, 227, 236, 251
Cfr13I GGNCC 1 cut(s) 33
CseI GACGC 2 cut(s) 178, 229
CviAII CATG 4 cut(s) 133, 138, 171, 237
CviJI RGCY 8 cut(s) 35, 39, 57, 162, 200, 209, 231, 254
CviKI_1 RGCY 8 cut(s) 35, 39, 57, 162, 200, 209, 231, 254
DdeI CTNAG 2 cut(s) 29, 183
DinI GGCGCC 1 cut(s) 82
Eco31I GGTCTC 1 cut(s) 32
Eco72I CACGTG 1 cut(s) 219
EgeI GGCGCC 1 cut(s) 82
EheI GGCGCC 1 cut(s) 82
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 4 cut(s) 136, 141, 174, 240
FaiI YATR 4 cut(s) 134, 139, 172, 238
FatI CATG 4 cut(s) 132, 137, 170, 236
FauI CCCGC 3 cut(s) 13, 113, 220
Fnu4HI GCNGC 7 cut(s) 40, 55, 58, 160, 163, 232, 252
Fsp4HI GCNGC 7 cut(s) 40, 55, 58, 160, 163, 232, 252
FspI TGCGCA 2 cut(s) 235, 250
GlaI GCGC 5 cut(s) 82, 181, 226, 235, 250
GluI GCNGC 7 cut(s) 40, 55, 58, 160, 163, 232, 252
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 2 cut(s) 35, 209
HapII CCGG 1 cut(s) 75
HgaI GACGC 2 cut(s) 178, 229
HhaI GCGC 5 cut(s) 83, 182, 227, 236, 251
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 4 cut(s) 136, 141, 174, 240
Hin6I GCGC 5 cut(s) 81, 180, 225, 234, 249
HinP1I GCGC 5 cut(s) 81, 180, 225, 234, 249
HpaII CCGG 1 cut(s) 75
Hpy166II GTNNAC 1 cut(s) 96
Hpy188I TCNGA 1 cut(s) 109
Hpy8I GTNNAC 1 cut(s) 96
Hpy99I CGWCG 1 cut(s) 194
HpyCH4IV ACGT 1 cut(s) 218
HpyCH4V TGCA 8 cut(s) 42, 60, 90, 96, 159, 165, 174, 216
HpyF3I CTNAG 2 cut(s) 29, 183
HpySE526I ACGT 1 cut(s) 218
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 4 cut(s) 136, 141, 174, 240
HspAI GCGC 5 cut(s) 81, 180, 225, 234, 249
KasI GGCGCC 1 cut(s) 80
LmnI GCTCC 1 cut(s) 146
LpnPI CCDG 5 cut(s) 16, 49, 88, 157, 186
Lsp1109I GCAGC 5 cut(s) 26, 44, 149, 171, 218
LweI GCATC 1 cut(s) 41
MaeII ACGT 1 cut(s) 218
MaeIII GTNAC 1 cut(s) 193
MhlI GDGCHC 3 cut(s) 74, 98, 156
Mly113I GGCGCC 1 cut(s) 81
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 2 cut(s) 94, 115
MspA1I CMGCKG 3 cut(s) 20, 39, 162
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 75
MvnI CGCG 1 cut(s) 227
NarI GGCGCC 1 cut(s) 81
NciI CCSGG 1 cut(s) 75
NlaIII CATG 4 cut(s) 136, 141, 174, 240
NlaIV GGNNCC 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 193
NsbI TGCGCA 2 cut(s) 235, 250
NspI RCATGY 2 cut(s) 141, 240
PaeI GCATGC 1 cut(s) 240
PkrI GCNGC 7 cut(s) 41, 56, 59, 161, 164, 233, 253
PluTI GGCGCC 1 cut(s) 84
PmaCI CACGTG 1 cut(s) 219
PmlI CACGTG 1 cut(s) 219
Ppu21I YACGTR 1 cut(s) 219
PspCI CACGTG 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 82
PspPI GGNCC 1 cut(s) 33
PstI CTGCAG 1 cut(s) 161
PvuII CAGCTG 2 cut(s) 39, 162
SatI GCNGC 7 cut(s) 40, 55, 58, 160, 163, 232, 252
Sau96I GGNCC 1 cut(s) 33
ScrFI CCNGG 1 cut(s) 75
SduI GDGCHC 3 cut(s) 74, 98, 156
SetI ASST 4 cut(s) 41, 107, 164, 221
SfaNI GCATC 1 cut(s) 41
SfcI CTRYAG 1 cut(s) 157
SfoI GGCGCC 1 cut(s) 82
SphI GCATGC 1 cut(s) 240
SsiI CCGC 4 cut(s) 20, 54, 120, 227
SspDI GGCGCC 1 cut(s) 80
StyD4I CCNGG 1 cut(s) 73
TaiI ACGT 1 cut(s) 221
TaqII GACCGA 1 cut(s) 63
TauI GCSGC 1 cut(s) 57
TscAI CASTG 1 cut(s) 161
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 6 cut(s) 39, 57, 159, 162, 231, 251
Tsp45I GTSAC 1 cut(s) 193
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 1 cut(s) 161
VneI GTGCAC 1 cut(s) 94
XceI RCATGY 2 cut(s) 141, 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.