Rh6DG242800

EC protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0009194
Physical Location & Seq
Reverse (-)
42997378 .. 42999917
2540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG242800.1

Sequence Viewer

Length: 354 bp
ATGGCAGAAACAGTTGGAGGTGTGAAGATGTCCTGCAACGATAGCTGTGGCTGCCCTGTTCCGTGCCCTGGCGGCGTGTCCTGTAGGTGCATAACCAGGACGGAGGCCACGAGTGGGGACGGCCACATGAAGTGCTCGTGCGGCGAGCACTGTGGCTGCAACCCGTGCGCTTGTGCTAAGAGCGTCGTCTCTACCGGAGTAGGCAGGGCCTACTGCAGGTGCGGCGAGGGTTGTACCTGTGACACTGCAGAAGTGCTTCTAGTCCATACAAAGAAAGCCTTGAGAAATTTTGCTAGCTCAAGCAGGGACAAGACTATGTACATGTTCTCTAAAATCGAACAACTACCTGCATGA

Protein Analysis

117

Amino Acids

12.2

Weight (kDa)

8.15

Isoelectric Point (pI)

60.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_PEC PF02068 12 - 83 5.2e-24 Plant PEC family metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 207
AciI CCGC 3 cut(s) 72, 141, 222
AcoI YGGCCR 1 cut(s) 121
AcsI RAATTY 1 cut(s) 286
AfaI GTAC 2 cut(s) 235, 320
AfiI CCNNNNNNNGG 3 cut(s) 68, 114, 216
AflIII ACRYGT 1 cut(s) 321
AjnI CCWGG 2 cut(s) 67, 95
AluBI AGCT 2 cut(s) 45, 297
AluI AGCT 2 cut(s) 45, 297
Alw21I GWGCWC 2 cut(s) 137, 150
Alw26I GTCTC 1 cut(s) 193
AoxI GGCC 3 cut(s) 105, 121, 207
ApeKI GCWGC 2 cut(s) 51, 156
ApoI RAATTY 1 cut(s) 286
Asp700I GAANNNNTTC 1 cut(s) 255
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 1 cut(s) 207
AsuNHI GCTAGC 1 cut(s) 293
BaeGI GKGCMC 1 cut(s) 68
BauI CACGAG 2 cut(s) 109, 136
Bbv12I GWGCWC 2 cut(s) 137, 150
BbvI GCAGC 2 cut(s) 38, 143
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 2 cut(s) 69, 97
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 2 cut(s) 260, 294
BfmI CTRYAG 3 cut(s) 82, 214, 246
BfuAI ACCTGC 1 cut(s) 207
BglI GCCNNNNNGGC 1 cut(s) 72
BisI GCNGC 5 cut(s) 52, 73, 142, 157, 223
BlsI GCNGC 5 cut(s) 53, 74, 143, 158, 224
Bme1390I CCNGG 2 cut(s) 69, 97
BmgT120I GGNCC 1 cut(s) 207
BmrFI CCNGG 2 cut(s) 69, 97
BmtI GCTAGC 1 cut(s) 297
BpuEI CTTGAG 2 cut(s) 283, 301
BsaJI CCNNGG 1 cut(s) 67
BsaWI WCCGGW 1 cut(s) 194
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 114, 216
BseBI CCWGG 2 cut(s) 69, 97
BseDI CCNNGG 1 cut(s) 67
BseLI CCNNNNNNNGG 3 cut(s) 68, 114, 216
BseSI GKGCMC 1 cut(s) 68
BseXI GCAGC 2 cut(s) 38, 143
BshFI GGCC 3 cut(s) 107, 123, 209
BsiHKAI GWGCWC 2 cut(s) 137, 150
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 2 cut(s) 131, 320
BslI CCNNNNNNNGG 3 cut(s) 68, 114, 216
BsmAI GTCTC 1 cut(s) 193
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 2 cut(s) 131, 320
BsnI GGCC 3 cut(s) 107, 123, 209
Bsp1286I GDGCHC 3 cut(s) 68, 137, 150
Bsp1407I TGTACA 1 cut(s) 318
BspACI CCGC 3 cut(s) 72, 141, 222
BspANI GGCC 3 cut(s) 107, 123, 209
BspMAI CTGCAG 2 cut(s) 218, 250
BspMI ACCTGC 1 cut(s) 207
BspOI GCTAGC 1 cut(s) 297
BsrGI TGTACA 1 cut(s) 318
BssECI CCNNGG 1 cut(s) 67
BssSI CACGAG 2 cut(s) 109, 136
Bst2BI CACGAG 2 cut(s) 109, 136
Bst2UI CCWGG 2 cut(s) 69, 97
Bst4CI ACNGT 2 cut(s) 13, 152
BstAPI GCANNNNNTGC 1 cut(s) 165
BstAUI TGTACA 1 cut(s) 318
BstC8I GCNNGC 2 cut(s) 146, 295
BstDEI CTNAG 1 cut(s) 177
BstENI CCTNNNNNAGG 1 cut(s) 214
BstHHI GCGC 1 cut(s) 170
BstMAI GTCTC 1 cut(s) 193
BstMWI GCNNNNNNNGC 6 cut(s) 42, 51, 72, 141, 165, 222
BstNI CCWGG 2 cut(s) 69, 97
BstNSI RCATGY 1 cut(s) 325
BstSCI CCNGG 2 cut(s) 67, 95
BstSFI CTRYAG 3 cut(s) 82, 214, 246
BstSLI GKGCMC 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 38, 143
BsuRI GGCC 3 cut(s) 107, 123, 209
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 2 cut(s) 148, 243
BveI ACCTGC 1 cut(s) 207
Cac8I GCNNGC 2 cut(s) 146, 295
CfoI GCGC 1 cut(s) 170
Cfr13I GGNCC 1 cut(s) 207
CseI GACGC 1 cut(s) 172
Csp6I GTAC 2 cut(s) 234, 319
CviAII CATG 3 cut(s) 127, 322, 351
CviJI RGCY 8 cut(s) 45, 51, 107, 123, 156, 209, 278, 297
CviKI_1 RGCY 8 cut(s) 45, 51, 107, 123, 156, 209, 278, 297
CviQI GTAC 2 cut(s) 234, 319
DdeI CTNAG 1 cut(s) 177
EaeI YGGCCR 1 cut(s) 121
EcoNI CCTNNNNNAGG 1 cut(s) 214
EcoO109I RGGNCCY 1 cut(s) 207
EcoRII CCWGG 2 cut(s) 67, 95
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 3 cut(s) 130, 325, 354
FaiI YATR 6 cut(s) 92, 128, 267, 317, 323, 352
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 2 cut(s) 131, 320
FatI CATG 3 cut(s) 126, 321, 350
Fnu4HI GCNGC 5 cut(s) 52, 73, 142, 157, 223
Fsp4HI GCNGC 5 cut(s) 52, 73, 142, 157, 223
FspBI CTAG 2 cut(s) 260, 294
GlaI GCGC 1 cut(s) 169
GluI GCNGC 5 cut(s) 52, 73, 142, 157, 223
HaeIII GGCC 3 cut(s) 107, 123, 209
HapII CCGG 1 cut(s) 195
HgaI GACGC 1 cut(s) 172
HhaI GCGC 1 cut(s) 170
Hin1II CATG 3 cut(s) 130, 325, 354
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HpaII CCGG 1 cut(s) 195
Hpy99I CGWCG 1 cut(s) 188
HpyCH4III ACNGT 2 cut(s) 13, 152
HpyCH4V TGCA 6 cut(s) 36, 90, 159, 216, 248, 350
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 51, 72, 141, 165, 222
HpyF3I CTNAG 1 cut(s) 177
Hsp92II CATG 3 cut(s) 130, 325, 354
HspAI GCGC 1 cut(s) 168
Lsp1109I GCAGC 2 cut(s) 38, 143
MaeI CTAG 2 cut(s) 260, 294
MaeIII GTNAC 1 cut(s) 239
MboII GAAGA 1 cut(s) 37
MhlI GDGCHC 3 cut(s) 68, 137, 150
MluCI AATT 1 cut(s) 286
MnlI CCTC 3 cut(s) 11, 97, 220
MroXI GAANNNNTTC 1 cut(s) 255
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 2 cut(s) 69, 97
MvaI CCWGG 2 cut(s) 69, 97
MwoI GCNNNNNNNGC 6 cut(s) 42, 51, 72, 141, 165, 222
NheI GCTAGC 1 cut(s) 293
NlaIII CATG 3 cut(s) 130, 325, 354
NmuCI GTSAC 1 cut(s) 239
NspI RCATGY 1 cut(s) 325
PaqCI CACCTGC 1 cut(s) 207
PciI ACATGT 1 cut(s) 321
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 255
PkrI GCNGC 5 cut(s) 53, 74, 143, 158, 224
PscI ACATGT 1 cut(s) 321
Psp6I CCWGG 2 cut(s) 67, 95
PspGI CCWGG 2 cut(s) 67, 95
PspPI GGNCC 1 cut(s) 207
PstI CTGCAG 2 cut(s) 218, 250
RsaI GTAC 2 cut(s) 235, 320
RsaNI GTAC 2 cut(s) 234, 319
SatI GCNGC 5 cut(s) 52, 73, 142, 157, 223
Sau96I GGNCC 1 cut(s) 207
ScrFI CCNGG 2 cut(s) 69, 97
SduI GDGCHC 3 cut(s) 68, 137, 150
SetI ASST 7 cut(s) 22, 47, 89, 221, 239, 299, 349
SfcI CTRYAG 3 cut(s) 82, 214, 246
SmlI CTYRAG 2 cut(s) 280, 298
SmoI CTYRAG 2 cut(s) 280, 298
Sse9I AATT 1 cut(s) 286
SsiI CCGC 3 cut(s) 72, 141, 222
SspMI CTAG 2 cut(s) 260, 294
StyD4I CCNGG 2 cut(s) 67, 95
TaaI ACNGT 2 cut(s) 13, 152
TaqI TCGA 1 cut(s) 336
TasI AATT 1 cut(s) 286
TatI WGTACW 1 cut(s) 318
TauI GCSGC 3 cut(s) 75, 144, 225
TscAI CASTG 2 cut(s) 155, 250
TseFI GTSAC 1 cut(s) 239
TseI GCWGC 2 cut(s) 51, 156
Tsp45I GTSAC 1 cut(s) 239
TspDTI ATGAA 1 cut(s) 143
TspGWI ACGGA 2 cut(s) 51, 116
TspRI CASTG 2 cut(s) 155, 250
XagI CCTNNNNNAGG 1 cut(s) 214
XapI RAATTY 1 cut(s) 286
XceI RCATGY 1 cut(s) 325
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 2 cut(s) 260, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.