FvH4_2g19010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004444
Physical Location & Seq
Forward (+)
16168623 .. 16175174
6552 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19010.t1

Sequence Viewer

Length: 1134 bp
ATGGCCGTTTTTGCACCCGGGTCCTCTCTATTCCCACACACCCCAAAACCCAAATTCTCAAATCCTCAACTTGTCAGGCATTCAAGCTGTTTCAATGTCAGACACAGATTGCTGGTGAATGGGAAACAATGTAGGCTAGTTTCCCAGTTCTTCAGACCCCAAACTTTCAGGATTAGAAGCTTTTCAAAGGAGGATTCAGAAAATGATGAGGCTGCAATGTCTAATGACATTCCGGATGCGCAACATGGGATTGATGATGACCTGCTTGGAAGTGTTAAGATTACTCAGACTGGTACTGAAACTTCATTTCTGGCTAAAATAGCTATTGCACTGGGCATAGCTGCAACAGTTACATTGATATCTATCTGCATCAAGCGTCCCACTCTTGGATCATCGAATGGGATTCAGTTCTTGCCAGAAAGCTCATCCTCATCAGCAATGGCAGCGGCCCCTGTTGGATTTACTTTCAAAGTTTTTGGCTACAGCATTGTGCTTCCAGAATATGCACCAGGGTGGATCTACTTTTGGTTGCTTATGGCTGCAGGGTTTGGGCTTTTCATCAGTGAAGAAGCTCTAAATATATGGGTCGGCATATCTTTGGCCCGGAGGCTATCTTTGGATGGGACCTGGCAGTCATTCGCTGAATCTTTCTCGAGAAATGCTCCGTACATTATATCTACAACACTTTGGGTGTATTGGGGAGTATGCATTAGTGATCTAATACCATTTTATCTGGGGAAGCTTTTTAGACAATCCAGGGCGTCAGATGATGTTCTTTCAAAGTTGGGGATTGGCAAAGAGAAAGCGTTAAGCATAACCCGTGCGGTGCAAAGATATGGCAATCTAATAGGTTTTGTTGAACGCTTTTCCCTTGGAGTCAGAAATCCAACAGCCTTTCTAGCGGGAACCTTGGGTATATCTCCAGAGTGTTTCTTTGCTGGAGTCTGTTTGGGTGGATTGATCACCCTTCCAATCCAGTTCGGGATAGGATTCTTCTTGAGGGAGCGCCCTGTCTTTGCCCTAGCTACTGTTGCTACAGTTGTGGGGATTTGGACTATTTTTCCTTATGCAGTGGCTACATCAACAGCATTATTCCTTTACCTTAGACAACGGTACTCTACATCGTCAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

378

Amino Acids

41.38

Weight (kDa)

9.23

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 226 - 329 4.6e-09 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 631
Acc16I TGCGCA 1 cut(s) 240
Acc36I ACCTGC 1 cut(s) 270
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 3 cut(s) 446, 824, 902
AclWI GGATC 2 cut(s) 397, 524
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 53
AcuI CTGAAG 1 cut(s) 136
AcyI GRCGYC 1 cut(s) 761
AfaI GTAC 3 cut(s) 295, 668, 1115
AfiI CCNNNNNNNGG 1 cut(s) 386
AgsI TTSAA 6 cut(s) 84, 94, 186, 469, 780, 860
AjnI CCWGG 3 cut(s) 508, 626, 755
AleI CACNNNNGTG 1 cut(s) 511
AluBI AGCT 8 cut(s) 87, 180, 323, 341, 423, 572, 742, 1025
AluI AGCT 8 cut(s) 87, 180, 323, 341, 423, 572, 742, 1025
AlwI GGATC 2 cut(s) 397, 524
Ama87I CYCGRG 2 cut(s) 17, 652
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 3 cut(s) 3, 447, 600
ApeKI GCWGC 4 cut(s) 212, 341, 443, 539
ApoI RAATTY 1 cut(s) 53
Asp700I GAANNNNTTC 1 cut(s) 181
AspLEI GCGC 2 cut(s) 241, 1008
AspS9I GGNCC 4 cut(s) 21, 448, 601, 624
AsuC2I CCSGG 3 cut(s) 18, 19, 604
AsuHPI GGTGA 2 cut(s) 127, 955
AvaI CYCGRG 2 cut(s) 17, 652
AvaII GGWCC 2 cut(s) 21, 624
BarI GAAGNNNNNNTAC 2 cut(s) 286, 318
BbvI GCAGC 4 cut(s) 199, 328, 455, 526
BccI CCATC 1 cut(s) 614
BciT130I CCWGG 3 cut(s) 510, 628, 757
BclI TGATCA 1 cut(s) 960
BcnI CCSGG 3 cut(s) 18, 19, 604
BfaI CTAG 3 cut(s) 137, 899, 1022
BfmI CTRYAG 3 cut(s) 481, 540, 1035
BfoI RGCGCY 1 cut(s) 1009
BfuAI ACCTGC 1 cut(s) 270
BisI GCNGC 5 cut(s) 213, 342, 444, 447, 540
BlsI GCNGC 5 cut(s) 214, 343, 445, 448, 541
Bme1390I CCNGG 6 cut(s) 18, 19, 510, 604, 628, 757
Bme18I GGWCC 2 cut(s) 21, 624
BmeT110I CYCGRG 2 cut(s) 17, 652
BmgT120I GGNCC 4 cut(s) 21, 448, 601, 624
BmiI GGNNCC 4 cut(s) 22, 450, 625, 907
BmrFI CCNGG 6 cut(s) 18, 19, 510, 604, 628, 757
BmrI ACTGGG 2 cut(s) 139, 341
BmsI GCATC 2 cut(s) 226, 378
BmuI ACTGGG 2 cut(s) 139, 341
BplI GAGNNNNNCTC 2 cut(s) 646, 678
BpmI CTGGAG 2 cut(s) 906, 960
BpuEI CTTGAG 1 cut(s) 1018
BpuMI CCSGG 3 cut(s) 18, 19, 604
BsaBI GATNNNNATC 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 761
BsaJI CCNNGG 5 cut(s) 17, 509, 756, 871, 909
BsaWI WCCGGW 1 cut(s) 232
Bsc4I CCNNNNNNNGG 1 cut(s) 386
Bse1I ACTGG 4 cut(s) 145, 295, 336, 976
Bse3DI GCAATG 2 cut(s) 222, 444
Bse8I GATNNNNATC 1 cut(s) 362
BseAI TCCGGA 1 cut(s) 232
BseBI CCWGG 3 cut(s) 510, 628, 757
BseDI CCNNGG 5 cut(s) 17, 509, 756, 871, 909
BseGI GGATG 3 cut(s) 241, 425, 625
BseJI GATNNNNATC 1 cut(s) 362
BseLI CCNNNNNNNGG 1 cut(s) 386
BseMI GCAATG 2 cut(s) 222, 444
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 4 cut(s) 145, 295, 336, 976
BseXI GCAGC 4 cut(s) 199, 328, 455, 526
BshFI GGCC 3 cut(s) 5, 449, 602
BsiHKCI CYCGRG 2 cut(s) 17, 652
BsiSI CCGG 3 cut(s) 18, 233, 604
BslFI GGGAC 2 cut(s) 363, 637
BslI CCNNNNNNNGG 1 cut(s) 386
BsmFI GGGAC 2 cut(s) 363, 637
BsmI GAATGC 1 cut(s) 79
BsnI GGCC 3 cut(s) 5, 449, 602
BsoBI CYCGRG 2 cut(s) 17, 652
Bsp13I TCCGGA 1 cut(s) 232
Bsp143I GATC 4 cut(s) 389, 516, 715, 960
BspACI CCGC 3 cut(s) 446, 824, 902
BspANI GGCC 3 cut(s) 5, 449, 602
BspCNI CTCAG 1 cut(s) 298
BspEI TCCGGA 1 cut(s) 232
BspLI GGNNCC 4 cut(s) 22, 450, 625, 907
BspMAI CTGCAG 1 cut(s) 544
BspMI ACCTGC 1 cut(s) 270
BspPI GGATC 2 cut(s) 397, 524
BsrDI GCAATG 2 cut(s) 222, 444
BsrI ACTGG 4 cut(s) 145, 295, 336, 976
BssECI CCNNGG 5 cut(s) 17, 509, 756, 871, 909
BssMI GATC 4 cut(s) 389, 516, 715, 960
BssNI GRCGYC 1 cut(s) 761
BssT1I CCWWGG 2 cut(s) 871, 909
Bst2UI CCWGG 3 cut(s) 510, 628, 757
Bst4CI ACNGT 4 cut(s) 349, 1030, 1039, 1113
BstACI GRCGYC 1 cut(s) 761
BstDEI CTNAG 2 cut(s) 285, 1103
BstF5I GGATG 3 cut(s) 241, 425, 625
BstH2I RGCGCY 1 cut(s) 1009
BstHHI GCGC 2 cut(s) 241, 1008
BstKTI GATC 4 cut(s) 392, 519, 718, 963
BstMBI GATC 4 cut(s) 389, 516, 715, 960
BstMWI GCNNNNNNNGC 5 cut(s) 11, 320, 443, 899, 1031
BstNI CCWGG 3 cut(s) 510, 628, 757
BstSCI CCNGG 6 cut(s) 16, 17, 508, 602, 626, 755
BstSFI CTRYAG 3 cut(s) 481, 540, 1035
BstV1I GCAGC 4 cut(s) 199, 328, 455, 526
BstX2I RGATCY 1 cut(s) 516
BstYI RGATCY 1 cut(s) 516
BsuRI GGCC 3 cut(s) 5, 449, 602
BtsCI GGATG 3 cut(s) 241, 425, 625
BtsI GCAGTG 1 cut(s) 1077
BtsIMutI CAGTG 3 cut(s) 329, 568, 1077
BveI ACCTGC 1 cut(s) 270
CfoI GCGC 2 cut(s) 241, 1008
Cfr13I GGNCC 4 cut(s) 21, 448, 601, 624
Cfr9I CCCGGG 1 cut(s) 17
CseI GACGC 2 cut(s) 365, 750
Csp6I GTAC 3 cut(s) 294, 667, 1114
CviAII CATG 1 cut(s) 245
CviQI GTAC 3 cut(s) 294, 667, 1114
DdeI CTNAG 2 cut(s) 285, 1103
DpnI GATC 4 cut(s) 391, 518, 717, 962
DpnII GATC 4 cut(s) 389, 516, 715, 960
DrdI GACNNNNNNGTC 1 cut(s) 631
DseDI GACNNNNNNGTC 1 cut(s) 631
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 2 cut(s) 871, 909
Eco32I GATATC 1 cut(s) 360
Eco47I GGWCC 2 cut(s) 21, 624
Eco57I CTGAAG 1 cut(s) 136
Eco88I CYCGRG 2 cut(s) 17, 652
EcoO109I RGGNCCY 2 cut(s) 21, 624
EcoRII CCWGG 3 cut(s) 508, 626, 755
EcoRV GATATC 1 cut(s) 360
EcoT14I CCWWGG 2 cut(s) 871, 909
EcoT22I ATGCAT 1 cut(s) 710
ErhI CCWWGG 2 cut(s) 871, 909
FaeI CATG 1 cut(s) 248
FaqI GGGAC 2 cut(s) 363, 637
FatI CATG 1 cut(s) 244
FauI CCCGC 1 cut(s) 895
FbaI TGATCA 1 cut(s) 960
Fnu4HI GCNGC 5 cut(s) 213, 342, 444, 447, 540
FokI GGATG 3 cut(s) 248, 412, 632
Fsp4HI GCNGC 5 cut(s) 213, 342, 444, 447, 540
FspBI CTAG 3 cut(s) 137, 899, 1022
FspI TGCGCA 1 cut(s) 240
GlaI GCGC 2 cut(s) 240, 1007
GluI GCNGC 5 cut(s) 213, 342, 444, 447, 540
GsuI CTGGAG 2 cut(s) 906, 960
HaeII RGCGCY 1 cut(s) 1009
HaeIII GGCC 3 cut(s) 5, 449, 602
HapII CCGG 3 cut(s) 18, 233, 604
HgaI GACGC 2 cut(s) 365, 750
HhaI GCGC 2 cut(s) 241, 1008
Hin1I GRCGYC 1 cut(s) 761
Hin1II CATG 1 cut(s) 248
Hin6I GCGC 2 cut(s) 239, 1006
HinP1I GCGC 2 cut(s) 239, 1006
HindIII AAGCTT 2 cut(s) 178, 740
HinfI GANTC 6 cut(s) 194, 403, 644, 876, 942, 990
HpaII CCGG 3 cut(s) 18, 233, 604
HphI GGTGA 2 cut(s) 127, 955
Hpy188I TCNGA 7 cut(s) 101, 155, 199, 288, 766, 881, 1129
Hpy188III TCNNGA 8 cut(s) 169, 233, 497, 652, 654, 923, 982, 997
HpyAV CCTTC 1 cut(s) 977
HpyCH4III ACNGT 4 cut(s) 349, 1030, 1039, 1113
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 320, 443, 899, 1031
HpyF3I CTNAG 2 cut(s) 285, 1103
Hsp92I GRCGYC 1 cut(s) 761
Hsp92II CATG 1 cut(s) 248
HspAI GCGC 2 cut(s) 239, 1006
Kpn2I TCCGGA 1 cut(s) 232
Ksp22I TGATCA 1 cut(s) 960
Kzo9I GATC 4 cut(s) 389, 516, 715, 960
LmnI GCTCC 2 cut(s) 667, 1003
Lsp1109I GCAGC 4 cut(s) 199, 328, 455, 526
LweI GCATC 2 cut(s) 226, 378
MaeI CTAG 3 cut(s) 137, 899, 1022
MaeIII GTNAC 1 cut(s) 349
MalI GATC 4 cut(s) 391, 518, 717, 962
MboI GATC 4 cut(s) 389, 516, 715, 960
MboII GAAGA 3 cut(s) 142, 578, 985
MflI RGATCY 1 cut(s) 516
MluCI AATT 1 cut(s) 53
MlyI GAGTC 2 cut(s) 885, 951
MmeI TCCRAC 2 cut(s) 436, 911
MnlI CCTC 7 cut(s) 34, 75, 184, 202, 439, 600, 993
Mph1103I ATGCAT 1 cut(s) 710
MroI TCCGGA 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 181
MseI TTAA 2 cut(s) 276, 809
MslI CAYNNNNRTG 1 cut(s) 511
MspA1I CMGCKG 1 cut(s) 446
MspI CCGG 3 cut(s) 18, 233, 604
MspR9I CCNGG 6 cut(s) 18, 19, 510, 604, 628, 757
Mva1269I GAATGC 1 cut(s) 79
MvaI CCWGG 3 cut(s) 510, 628, 757
MwoI GCNNNNNNNGC 5 cut(s) 11, 320, 443, 899, 1031
NciI CCSGG 3 cut(s) 18, 19, 604
NdeII GATC 4 cut(s) 389, 516, 715, 960
NlaIII CATG 1 cut(s) 248
NlaIV GGNNCC 4 cut(s) 22, 450, 625, 907
NsbI TGCGCA 1 cut(s) 240
NsiI ATGCAT 1 cut(s) 710
OliI CACNNNNGTG 1 cut(s) 511
PaeR7I CTCGAG 1 cut(s) 652
PctI GAATGC 1 cut(s) 79
PdmI GAANNNNTTC 1 cut(s) 181
PfeI GAWTC 4 cut(s) 194, 403, 644, 990
PkrI GCNGC 5 cut(s) 214, 343, 445, 448, 541
PleI GAGTC 2 cut(s) 884, 950
PpsI GAGTC 2 cut(s) 884, 950
PpuMI RGGWCCY 2 cut(s) 21, 624
Psp5II RGGWCCY 2 cut(s) 21, 624
Psp6I CCWGG 3 cut(s) 508, 626, 755
PspGI CCWGG 3 cut(s) 508, 626, 755
PspN4I GGNNCC 4 cut(s) 22, 450, 625, 907
PspPI GGNCC 4 cut(s) 21, 448, 601, 624
PspPPI RGGWCCY 2 cut(s) 21, 624
PstI CTGCAG 1 cut(s) 544
PsuI RGATCY 1 cut(s) 516
RsaI GTAC 3 cut(s) 295, 668, 1115
RsaNI GTAC 3 cut(s) 294, 667, 1114
RseI CAYNNNNRTG 1 cut(s) 511
SaqAI TTAA 2 cut(s) 276, 809
SatI GCNGC 5 cut(s) 213, 342, 444, 447, 540
Sau3AI GATC 4 cut(s) 389, 516, 715, 960
Sau96I GGNCC 4 cut(s) 21, 448, 601, 624
SchI GAGTC 2 cut(s) 885, 951
ScrFI CCNGG 6 cut(s) 18, 19, 510, 604, 628, 757
SfaNI GCATC 2 cut(s) 226, 378
SfcI CTRYAG 3 cut(s) 481, 540, 1035
Sfr274I CTCGAG 1 cut(s) 652
SinI GGWCC 2 cut(s) 21, 624
SlaI CTCGAG 1 cut(s) 652
SmaI CCCGGG 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 511
SmlI CTYRAG 2 cut(s) 652, 997
SmoI CTYRAG 2 cut(s) 652, 997
Sse9I AATT 1 cut(s) 53
SsiI CCGC 3 cut(s) 446, 824, 902
SspMI CTAG 3 cut(s) 137, 899, 1022
StyD4I CCNGG 6 cut(s) 16, 17, 508, 602, 626, 755
StyI CCWWGG 2 cut(s) 871, 909
TaaI ACNGT 4 cut(s) 349, 1030, 1039, 1113
TaqI TCGA 2 cut(s) 395, 653
TasI AATT 1 cut(s) 53
TauI GCSGC 1 cut(s) 449
TfiI GAWTC 4 cut(s) 194, 403, 644, 990
Tru1I TTAA 2 cut(s) 276, 809
Tru9I TTAA 2 cut(s) 276, 809
TscAI CASTG 3 cut(s) 336, 568, 1077
TseI GCWGC 4 cut(s) 212, 341, 443, 539
TspDTI ATGAA 2 cut(s) 294, 547
TspGWI ACGGA 1 cut(s) 654
TspMI CCCGGG 1 cut(s) 17
TspRI CASTG 3 cut(s) 336, 568, 1077
VpaK11BI GGWCC 2 cut(s) 21, 624
XapI RAATTY 1 cut(s) 53
XhoI CTCGAG 1 cut(s) 652
XmaI CCCGGG 1 cut(s) 17
XmnI GAANNNNTTC 1 cut(s) 181
XspI CTAG 3 cut(s) 137, 899, 1022
Zsp2I ATGCAT 1 cut(s) 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.