RLG00000012714

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004444
Physical Location & Seq
Reverse (-)
21556003 .. 21560859
4857 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012714

Sequence Viewer

Length: 1149 bp
ATGTCTCTAATGGCGGTTTTTGTACCTGGGTCTTCTCTATTCCCACGCACCCCAAAACCCAAATCTTCAAACCCACAACTTATTAGGCATTCAAGCTGTTTCGATGTCAGACACAGATTGCTGGTGAATTGGAAACAATGTGGGCTAGTTTCCCAGCTCTTCAGACCCCAAACGTTCAGGATTAGAAGCTTTTCGAAGGAGGATACAGAAAATGAAAATGATGAGGCTGCAATGTCTAATGACATTTTGAATGGGCAAGATGGGATTTATGATGAGTTGCTTGGAAATGTTAAAGTTACTCAGACTGCTACAGAAAGTCTATTTCTGGCTAAAATAGCGATTGCACTGGGAATAGCTGCAACAGTTACATTGATATCTATTGGCATCAAGGGTCCTACTCTTGGATCATCGAATGGGCTTCAGTTCTTGCCAGAAAGCCCATCCTCTTCAGCAATGGCGGCGGCCCCTGTTGGTTTCACTTTCAAAGCTTTTGGCTACAGCATTGTTCTTCCAGAATATGCACCAGGGTGGATCTACTTTTGGTTGCTTATGGCTGCAGGGTTTGGGCTTTTCATCAGTGAAGAGGCTCTGAATATATGGGTTGGCATATCTTTAGCCCGGAGGCTATCTTTGGATGGGACCTGGCAGTCATTTGCTGAATCTTTCTCGAGAAATGCTCCGTACATTGTATCTACAACTCTTTGGGTGTATTGGGGAGTATGCATTAGTGATCTAATACCTTTTTATATGGGGAAGCTTTTTAGACAATCCAGGGCGTCTGATGATATTTTTTCAAAGTTAGGAATTGGCAAAGAGAAAGCTTTAAGCATAACCCGTGCAGTGCAAAGATATGGCAACCTTATTGGTTTTGTTGAACGCTTTTCCCTTGGAGTGAGAAATCCAACAGCTTTTCTAGCAGGGACCTTAGGTATATCTCCAGAGTGTTTCTTTGCTGGAGTTTGTTTGGGTGGCTTGATCACCCTTCCGGTCCAGTTCGGGATAGGATTCTTCTTGAGGGAGCGCCCTGTCTTTGCCCTAGCTACTGTTGCAACAGTTGTGGGCATTTGGACTATTTTCCCTTATGCAGTGGCTACATCAACAGCATTGTTCCTTTACCTTCGACAACGTTACTCAACGTCGTCAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

383

Amino Acids

41.92

Weight (kDa)

9.05

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 231 - 334 1.6e-08 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 646
AciI CCGC 3 cut(s) 14, 458, 461
AclI AACGTT 2 cut(s) 173, 1126
AclWI GGATC 2 cut(s) 412, 539
AcuI CTGAAG 3 cut(s) 145, 404, 432
AcyI GRCGYC 1 cut(s) 776
AfaI GTAC 2 cut(s) 24, 683
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 6 cut(s) 69, 93, 250, 484, 795, 875
AjnI CCWGG 4 cut(s) 25, 523, 641, 770
AleI CACNNNNGTG 1 cut(s) 526
AluBI AGCT 9 cut(s) 96, 157, 189, 356, 488, 757, 821, 908, 1040
AluI AGCT 9 cut(s) 96, 157, 189, 356, 488, 757, 821, 908, 1040
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 2 cut(s) 412, 539
Ama87I CYCGRG 1 cut(s) 667
AoxI GGCC 1 cut(s) 462
ApeKI GCWGC 3 cut(s) 227, 356, 554
Asp700I GAANNNNTTC 1 cut(s) 190
AspLEI GCGC 1 cut(s) 1023
AspS9I GGNCC 5 cut(s) 392, 463, 639, 921, 988
AsuC2I CCSGG 1 cut(s) 619
AsuHPI GGTGA 2 cut(s) 136, 970
AsuII TTCGAA 1 cut(s) 194
AvaI CYCGRG 1 cut(s) 667
AvaII GGWCC 4 cut(s) 392, 639, 921, 988
AxyI CCTNAGG 1 cut(s) 925
BbsI GAAGAC 1 cut(s) 24
BbvI GCAGC 3 cut(s) 214, 343, 541
BccI CCATC 3 cut(s) 254, 448, 629
BciT130I CCWGG 4 cut(s) 27, 525, 643, 772
BciVI GTATCC 1 cut(s) 196
BclI TGATCA 1 cut(s) 975
BcnI CCSGG 1 cut(s) 619
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 3 cut(s) 146, 914, 1037
BfmI CTRYAG 3 cut(s) 309, 496, 555
BfoI RGCGCY 1 cut(s) 1024
BfuI GTATCC 1 cut(s) 196
BisI GCNGC 5 cut(s) 228, 357, 459, 462, 555
BlsI GCNGC 5 cut(s) 229, 358, 460, 463, 556
Bme1390I CCNGG 5 cut(s) 27, 525, 619, 643, 772
Bme18I GGWCC 4 cut(s) 392, 639, 921, 988
BmeT110I CYCGRG 1 cut(s) 667
BmgT120I GGNCC 5 cut(s) 392, 463, 639, 921, 988
BmiI GGNNCC 4 cut(s) 393, 465, 640, 922
BmrFI CCNGG 5 cut(s) 27, 525, 619, 643, 772
BmrI ACTGGG 1 cut(s) 356
BmsI GCATC 1 cut(s) 393
BmuI ACTGGG 1 cut(s) 356
BpiI GAAGAC 1 cut(s) 24
BplI GAGNNNNNCTC 2 cut(s) 661, 693
BpmI CTGGAG 2 cut(s) 921, 975
Bpu14I TTCGAA 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 1033
BpuMI CCSGG 1 cut(s) 619
BsaHI GRCGYC 1 cut(s) 776
BsaJI CCNNGG 4 cut(s) 26, 524, 771, 886
BsaWI WCCGGW 1 cut(s) 985
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse1I ACTGG 2 cut(s) 351, 991
Bse21I CCTNAGG 1 cut(s) 925
Bse3DI GCAATG 2 cut(s) 237, 459
BseBI CCWGG 4 cut(s) 27, 525, 643, 772
BseDI CCNNGG 4 cut(s) 26, 524, 771, 886
BseGI GGATG 2 cut(s) 440, 640
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 2 cut(s) 237, 459
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 2 cut(s) 351, 991
BseXI GCAGC 3 cut(s) 214, 343, 541
BseYI CCCAGC 1 cut(s) 153
BsgI GTGCAG 1 cut(s) 858
BshFI GGCC 1 cut(s) 464
BsiHKCI CYCGRG 1 cut(s) 667
BsiSI CCGG 2 cut(s) 619, 986
BslFI GGGAC 2 cut(s) 652, 934
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 652, 934
BsmI GAATGC 1 cut(s) 88
BsnI GGCC 1 cut(s) 464
BsoBI CYCGRG 1 cut(s) 667
Bsp119I TTCGAA 1 cut(s) 194
Bsp143I GATC 4 cut(s) 404, 531, 730, 975
BspACI CCGC 3 cut(s) 14, 458, 461
BspANI GGCC 1 cut(s) 464
BspCNI CTCAG 1 cut(s) 313
BspLI GGNNCC 4 cut(s) 393, 465, 640, 922
BspMAI CTGCAG 1 cut(s) 559
BspPI GGATC 2 cut(s) 412, 539
BspQI GCTCTTC 1 cut(s) 164
BspT104I TTCGAA 1 cut(s) 194
BsrDI GCAATG 2 cut(s) 237, 459
BsrI ACTGG 2 cut(s) 351, 991
BssECI CCNNGG 4 cut(s) 26, 524, 771, 886
BssMI GATC 4 cut(s) 404, 531, 730, 975
BssNI GRCGYC 1 cut(s) 776
BssT1I CCWWGG 1 cut(s) 886
Bst2UI CCWGG 4 cut(s) 27, 525, 643, 772
Bst4CI ACNGT 3 cut(s) 364, 1045, 1054
Bst6I CTCTTC 3 cut(s) 164, 451, 576
BstACI GRCGYC 1 cut(s) 776
BstBI TTCGAA 1 cut(s) 194
BstDEI CTNAG 2 cut(s) 300, 925
BstF5I GGATG 2 cut(s) 440, 640
BstH2I RGCGCY 1 cut(s) 1024
BstHHI GCGC 1 cut(s) 1023
BstKTI GATC 4 cut(s) 407, 534, 733, 978
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 4 cut(s) 404, 531, 730, 975
BstMWI GCNNNNNNNGC 4 cut(s) 335, 458, 914, 1046
BstNI CCWGG 4 cut(s) 27, 525, 643, 772
BstSCI CCNGG 5 cut(s) 25, 523, 617, 641, 770
BstSFI CTRYAG 3 cut(s) 309, 496, 555
BstV1I GCAGC 3 cut(s) 214, 343, 541
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 531
BstYI RGATCY 1 cut(s) 531
Bsu36I CCTNAGG 1 cut(s) 925
BsuI GTATCC 1 cut(s) 196
BsuRI GGCC 1 cut(s) 464
BtsCI GGATG 2 cut(s) 440, 640
BtsI GCAGTG 2 cut(s) 846, 1092
BtsIMutI CAGTG 4 cut(s) 344, 583, 846, 1092
CfoI GCGC 1 cut(s) 1023
Cfr13I GGNCC 5 cut(s) 392, 463, 639, 921, 988
CseI GACGC 1 cut(s) 765
Csp6I GTAC 2 cut(s) 23, 682
CspCI CAANNNNNGTGG 2 cut(s) 1038, 1073
CviQI GTAC 2 cut(s) 23, 682
DdeI CTNAG 2 cut(s) 300, 925
DpnI GATC 4 cut(s) 406, 533, 732, 977
DpnII GATC 4 cut(s) 404, 531, 730, 975
DrdI GACNNNNNNGTC 1 cut(s) 646
DseDI GACNNNNNNGTC 1 cut(s) 646
Eam1104I CTCTTC 3 cut(s) 164, 451, 576
EarI CTCTTC 3 cut(s) 164, 451, 576
Eco130I CCWWGG 1 cut(s) 886
Eco32I GATATC 1 cut(s) 375
Eco47I GGWCC 4 cut(s) 392, 639, 921, 988
Eco57I CTGAAG 3 cut(s) 145, 404, 432
Eco81I CCTNAGG 1 cut(s) 925
Eco88I CYCGRG 1 cut(s) 667
EcoO109I RGGNCCY 3 cut(s) 392, 639, 921
EcoRII CCWGG 4 cut(s) 25, 523, 641, 770
EcoRV GATATC 1 cut(s) 375
EcoT14I CCWWGG 1 cut(s) 886
EcoT22I ATGCAT 1 cut(s) 725
ErhI CCWWGG 1 cut(s) 886
FaqI GGGAC 2 cut(s) 652, 934
FbaI TGATCA 1 cut(s) 975
Fnu4HI GCNGC 5 cut(s) 228, 357, 459, 462, 555
FokI GGATG 2 cut(s) 427, 647
Fsp4HI GCNGC 5 cut(s) 228, 357, 459, 462, 555
FspBI CTAG 3 cut(s) 146, 914, 1037
GlaI GCGC 1 cut(s) 1022
GluI GCNGC 5 cut(s) 228, 357, 459, 462, 555
GsaI CCCAGC 1 cut(s) 157
GsuI CTGGAG 2 cut(s) 921, 975
HaeII RGCGCY 1 cut(s) 1024
HaeIII GGCC 1 cut(s) 464
HapII CCGG 2 cut(s) 619, 986
HgaI GACGC 1 cut(s) 765
HhaI GCGC 1 cut(s) 1023
Hin1I GRCGYC 1 cut(s) 776
Hin6I GCGC 1 cut(s) 1021
HinP1I GCGC 1 cut(s) 1021
HindIII AAGCTT 4 cut(s) 187, 486, 755, 819
HinfI GANTC 2 cut(s) 659, 1005
HpaII CCGG 2 cut(s) 619, 986
HphI GGTGA 2 cut(s) 136, 970
Hpy188I TCNGA 6 cut(s) 110, 164, 303, 591, 781, 1144
Hpy188III TCNNGA 7 cut(s) 178, 512, 667, 669, 938, 997, 1012
Hpy99I CGWCG 1 cut(s) 1141
HpyAV CCTTC 3 cut(s) 190, 992, 1127
HpyCH4III ACNGT 3 cut(s) 364, 1045, 1054
HpyCH4IV ACGT 3 cut(s) 173, 1126, 1136
HpyF10VI GCNNNNNNNGC 4 cut(s) 335, 458, 914, 1046
HpyF3I CTNAG 2 cut(s) 300, 925
HpySE526I ACGT 3 cut(s) 173, 1126, 1136
Hsp92I GRCGYC 1 cut(s) 776
HspAI GCGC 1 cut(s) 1021
Ksp22I TGATCA 1 cut(s) 975
Kzo9I GATC 4 cut(s) 404, 531, 730, 975
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 2 cut(s) 682, 1018
Lsp1109I GCAGC 3 cut(s) 214, 343, 541
LweI GCATC 1 cut(s) 393
MaeI CTAG 3 cut(s) 146, 914, 1037
MaeII ACGT 3 cut(s) 173, 1126, 1136
MaeIII GTNAC 3 cut(s) 295, 364, 1127
MalI GATC 4 cut(s) 406, 533, 732, 977
MboI GATC 4 cut(s) 404, 531, 730, 975
MboII GAAGA 7 cut(s) 24, 57, 151, 438, 500, 593, 1000
MflI RGATCY 1 cut(s) 531
MluCI AATT 2 cut(s) 127, 804
MmeI TCCRAC 1 cut(s) 926
MnlI CCTC 6 cut(s) 193, 217, 454, 577, 615, 1008
Mph1103I ATGCAT 1 cut(s) 725
MroXI GAANNNNTTC 1 cut(s) 190
MseI TTAA 2 cut(s) 291, 824
MslI CAYNNNNRTG 1 cut(s) 526
MspI CCGG 2 cut(s) 619, 986
MspR9I CCNGG 5 cut(s) 27, 525, 619, 643, 772
Mva1269I GAATGC 1 cut(s) 88
MvaI CCWGG 4 cut(s) 27, 525, 643, 772
MwoI GCNNNNNNNGC 4 cut(s) 335, 458, 914, 1046
NciI CCSGG 1 cut(s) 619
NdeII GATC 4 cut(s) 404, 531, 730, 975
NlaIV GGNNCC 4 cut(s) 393, 465, 640, 922
NsiI ATGCAT 1 cut(s) 725
NspV TTCGAA 1 cut(s) 194
OliI CACNNNNGTG 1 cut(s) 526
PaeR7I CTCGAG 1 cut(s) 667
PciSI GCTCTTC 1 cut(s) 164
PctI GAATGC 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 190
PfeI GAWTC 2 cut(s) 659, 1005
PkrI GCNGC 5 cut(s) 229, 358, 460, 463, 556
PpuMI RGGWCCY 3 cut(s) 392, 639, 921
Psp1406I AACGTT 2 cut(s) 173, 1126
Psp5II RGGWCCY 3 cut(s) 392, 639, 921
Psp6I CCWGG 4 cut(s) 25, 523, 641, 770
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 4 cut(s) 25, 523, 641, 770
PspN4I GGNNCC 4 cut(s) 393, 465, 640, 922
PspPI GGNCC 5 cut(s) 392, 463, 639, 921, 988
PspPPI RGGWCCY 3 cut(s) 392, 639, 921
PstI CTGCAG 1 cut(s) 559
PsuI RGATCY 1 cut(s) 531
RsaI GTAC 2 cut(s) 24, 683
RsaNI GTAC 2 cut(s) 23, 682
RseI CAYNNNNRTG 1 cut(s) 526
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 2 cut(s) 291, 824
SatI GCNGC 5 cut(s) 228, 357, 459, 462, 555
Sau3AI GATC 4 cut(s) 404, 531, 730, 975
Sau96I GGNCC 5 cut(s) 392, 463, 639, 921, 988
ScrFI CCNGG 5 cut(s) 27, 525, 619, 643, 772
SfaNI GCATC 1 cut(s) 393
SfcI CTRYAG 3 cut(s) 309, 496, 555
Sfr274I CTCGAG 1 cut(s) 667
SfuI TTCGAA 1 cut(s) 194
SinI GGWCC 4 cut(s) 392, 639, 921, 988
SlaI CTCGAG 1 cut(s) 667
SmiMI CAYNNNNRTG 1 cut(s) 526
SmlI CTYRAG 2 cut(s) 667, 1012
SmoI CTYRAG 2 cut(s) 667, 1012
Sse9I AATT 2 cut(s) 127, 804
SsiI CCGC 3 cut(s) 14, 458, 461
SspMI CTAG 3 cut(s) 146, 914, 1037
StyD4I CCNGG 5 cut(s) 25, 523, 617, 641, 770
StyI CCWWGG 1 cut(s) 886
TaaI ACNGT 3 cut(s) 364, 1045, 1054
TaiI ACGT 3 cut(s) 176, 1129, 1139
TaqI TCGA 5 cut(s) 102, 194, 410, 668, 1120
TasI AATT 2 cut(s) 127, 804
TauI GCSGC 2 cut(s) 461, 464
TfiI GAWTC 2 cut(s) 659, 1005
Tru1I TTAA 2 cut(s) 291, 824
Tru9I TTAA 2 cut(s) 291, 824
TscAI CASTG 4 cut(s) 351, 583, 846, 1092
TseI GCWGC 3 cut(s) 227, 356, 554
TspDTI ATGAA 2 cut(s) 228, 562
TspGWI ACGGA 1 cut(s) 669
TspRI CASTG 4 cut(s) 351, 583, 846, 1092
VpaK11BI GGWCC 4 cut(s) 392, 639, 921, 988
XhoI CTCGAG 1 cut(s) 667
XmnI GAANNNNTTC 1 cut(s) 190
XspI CTAG 3 cut(s) 146, 914, 1037
Zsp2I ATGCAT 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.