MD05G1174800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004444
Physical Location & Seq
Forward (+)
30278513 .. 30283608
5096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1174800.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGCGGTTTTGGCTCATGGGTTTTATCTACCCCCACCCACACGGACACCCAAATCGTCAAATCGGAAACTTGTTGGGTATTCGAGCTCTATCATTGTCAGACGCAGATTGCTGACGGACGGGAACGAATGCGTGCAGGTTTCCGAACTCTTCACTCCCAAGACTTTCAGGATCAGAAGCTTTTATGAGGAGGATGCAGGAAATAATGAGGTTGAAATATCTAAAGACATTCTGGATGGGGAAGATGGGATTTCGGATGAGCTGCTTGGAAATGTTGTGAATACTGAAATTAGTACAGAAAATTCATTTCTTGCTAAAATAGCAATTGCACTAGGCGTTGCCGCAACGGTGACTCTGATATCGGTTGGCATCAAGGGTGCACCCGCTATTGGGTCATCGAATGGAATTCAAATTTTTCCAGATAGTTCATCCTCTTCAGTAATGGCTTCAGCCAATGTCAGTTTCACCATCAAAGCTTTTGGGTACAGCATTGTTCTCCCAGCATTTGCACCGGGGTGGATCTACTTTTGGTTGCTTATGGCTGCAGGCTGTGGACTTTGCATTAGTGAAGAAGCTCTAAATATTTGGGTTGGCATATCTTTGGCTCGGATGCTGTCTTTGGACGGGACATGGCAGATGTTTGCTGAATCTTTTTCGAGAAATGCTCCATATATCATATCCACAATTCTTTGGGTGTATTGGGGAGTGTGCATCAGTGATCTAATACCATTTTATCTCGGGAAGCTTTTTAGAAAATCCAGGGCCTCTGATGACGTTTTCTCGAAGTTAGGGATTGGAAAAGAGAAAGCTTTAAGCATAACCTCTGCAGTGCAAAAATATGGCAACCTCATCGGTTTCGTTGAACGATTTTCGCTTGGAGTGAGAAATCCAACGGCTTTTCTAGCGGGGACCTTGGGCATTTCTTCAGAGTGTTTCTTTGCTGGAGTTTGTTGTGGCGGTTTGATCACCCTTCCCCTCCAGTTGTTAATAGGATTCTTCCTGAGGGAGCGCCCCGTCTTTGCCCTCGCTACTGTTGCAACAGCAATTGGCATTTGGACCATCTTCCCTTACGCTGTAGCTGCATCAACGGCATTATTCCTTTACCTGAGACGCCGCTACTCTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

376

Amino Acids

40.68

Weight (kDa)

8.41

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 227 - 331 7.8e-08 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 127
AciI CCGC 6 cut(s) 5, 342, 384, 905, 957, 1114
AclWI GGATC 2 cut(s) 179, 527
AcsI RAATTY 3 cut(s) 301, 405, 411
AcuI CTGAAG 3 cut(s) 420, 432, 909
AcyI GRCGYC 1 cut(s) 1111
AfaI GTAC 2 cut(s) 295, 485
AfiI CCNNNNNNNGG 2 cut(s) 389, 390
AgsI TTSAA 3 cut(s) 215, 410, 863
AjnI CCWGG 1 cut(s) 758
AjuI GAANNNNNNNTTGG 2 cut(s) 446, 478
AleI CACNNNNGTG 1 cut(s) 514
AluBI AGCT 8 cut(s) 87, 180, 262, 476, 575, 745, 809, 1079
AluI AGCT 8 cut(s) 87, 180, 262, 476, 575, 745, 809, 1079
Alw21I GWGCWC 2 cut(s) 89, 382
Alw26I GTCTC 1 cut(s) 1102
Alw44I GTGCAC 1 cut(s) 378
AlwI GGATC 2 cut(s) 179, 527
Ama87I CYCGRG 1 cut(s) 737
AoxI GGCC 1 cut(s) 762
ApaLI GTGCAC 1 cut(s) 378
ApeKI GCWGC 3 cut(s) 262, 542, 1079
ApoI RAATTY 3 cut(s) 301, 405, 411
AspLEI GCGC 1 cut(s) 1011
AspS9I GGNCC 3 cut(s) 762, 909, 1056
AsuC2I CCSGG 1 cut(s) 513
AsuHPI GGTGA 3 cut(s) 361, 457, 958
AvaI CYCGRG 1 cut(s) 737
AvaII GGWCC 2 cut(s) 909, 1056
AxyI CCTNAGG 1 cut(s) 1001
BaeGI GKGCMC 1 cut(s) 382
BanII GRGCYC 1 cut(s) 89
Bbv12I GWGCWC 2 cut(s) 89, 382
BbvI GCAGC 3 cut(s) 249, 529, 1066
BccI CCATC 4 cut(s) 230, 239, 476, 1067
BceAI ACGGC 2 cut(s) 909, 1104
BcgI CGANNNNNNTGC 2 cut(s) 832, 866
BciT130I CCWGG 1 cut(s) 760
BclI TGATCA 1 cut(s) 963
BcnI CCSGG 1 cut(s) 513
BcoDI GTCTC 1 cut(s) 1102
BfaI CTAG 3 cut(s) 332, 902, 1126
BfmI CTRYAG 3 cut(s) 543, 825, 1074
BfoI RGCGCY 1 cut(s) 1012
BfuAI ACCTGC 1 cut(s) 127
BisI GCNGC 5 cut(s) 263, 342, 543, 1080, 1114
BlsI GCNGC 5 cut(s) 264, 343, 544, 1081, 1115
Bme1390I CCNGG 2 cut(s) 513, 760
Bme18I GGWCC 2 cut(s) 909, 1056
BmeT110I CYCGRG 1 cut(s) 737
BmgT120I GGNCC 3 cut(s) 762, 909, 1056
BmiI GGNNCC 1 cut(s) 910
BmrFI CCNGG 2 cut(s) 513, 760
BmsI GCATC 5 cut(s) 184, 378, 600, 720, 1091
BplI GAGNNNNNCTC 2 cut(s) 649, 681
BpmI CTGGAG 2 cut(s) 962, 963
BpuMI CCSGG 1 cut(s) 513
BsaHI GRCGYC 1 cut(s) 1111
BsaJI CCNNGG 3 cut(s) 512, 759, 912
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 390
Bse1I ACTGG 1 cut(s) 979
Bse21I CCTNAGG 1 cut(s) 1001
BseBI CCWGG 1 cut(s) 760
BseDI CCNNGG 3 cut(s) 512, 759, 912
BseGI GGATG 5 cut(s) 199, 241, 262, 428, 615
BseLI CCNNNNNNNGG 2 cut(s) 389, 390
BseMII CTCAG 2 cut(s) 992, 1097
BseNI ACTGG 1 cut(s) 979
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 3 cut(s) 249, 529, 1066
BseYI CCCAGC 1 cut(s) 499
BsgI GTGCAG 1 cut(s) 155
BshFI GGCC 1 cut(s) 764
BsiHKAI GWGCWC 2 cut(s) 89, 382
BsiHKCI CYCGRG 1 cut(s) 737
BsiSI CCGG 1 cut(s) 512
BslFI GGGAC 2 cut(s) 640, 922
BslI CCNNNNNNNGG 2 cut(s) 389, 390
BsmAI GTCTC 1 cut(s) 1102
BsmBI CGTCTC 1 cut(s) 1102
BsmFI GGGAC 2 cut(s) 640, 922
BsmI GAATGC 1 cut(s) 134
BsnI GGCC 1 cut(s) 764
BsoBI CYCGRG 1 cut(s) 737
Bsp1286I GDGCHC 2 cut(s) 89, 382
Bsp143I GATC 4 cut(s) 171, 519, 718, 963
BspACI CCGC 6 cut(s) 5, 342, 384, 905, 957, 1114
BspANI GGCC 1 cut(s) 764
BspCNI CTCAG 2 cut(s) 993, 1098
BspLI GGNNCC 1 cut(s) 910
BspMAI CTGCAG 2 cut(s) 547, 829
BspMI ACCTGC 1 cut(s) 127
BspPI GGATC 2 cut(s) 179, 527
BsrI ACTGG 1 cut(s) 979
BssECI CCNNGG 3 cut(s) 512, 759, 912
BssMI GATC 4 cut(s) 171, 519, 718, 963
BssNI GRCGYC 1 cut(s) 1111
BssT1I CCWWGG 1 cut(s) 912
Bst2UI CCWGG 1 cut(s) 760
Bst4CI ACNGT 2 cut(s) 349, 1033
Bst6I CTCTTC 2 cut(s) 155, 439
BstACI GRCGYC 1 cut(s) 1111
BstC8I GCNNGC 2 cut(s) 134, 547
BstDEI CTNAG 2 cut(s) 1001, 1106
BstF5I GGATG 5 cut(s) 199, 241, 262, 428, 615
BstH2I RGCGCY 1 cut(s) 1012
BstHHI GCGC 1 cut(s) 1011
BstKTI GATC 4 cut(s) 174, 522, 721, 966
BstMAI GTCTC 1 cut(s) 1102
BstMBI GATC 4 cut(s) 171, 519, 718, 963
BstMWI GCNNNNNNNGC 6 cut(s) 11, 320, 902, 1034, 1079, 1088
BstNI CCWGG 1 cut(s) 760
BstSCI CCNGG 2 cut(s) 511, 758
BstSFI CTRYAG 3 cut(s) 543, 825, 1074
BstSLI GKGCMC 1 cut(s) 382
BstV1I GCAGC 3 cut(s) 249, 529, 1066
BstX2I RGATCY 1 cut(s) 519
BstYI RGATCY 1 cut(s) 519
Bsu36I CCTNAGG 1 cut(s) 1001
BsuRI GGCC 1 cut(s) 764
BtsCI GGATG 5 cut(s) 199, 241, 262, 428, 615
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 2 cut(s) 721, 834
BveI ACCTGC 1 cut(s) 127
Cac8I GCNNGC 2 cut(s) 134, 547
CfoI GCGC 1 cut(s) 1011
Cfr13I GGNCC 3 cut(s) 762, 909, 1056
CseI GACGC 2 cut(s) 111, 1119
Csp6I GTAC 2 cut(s) 294, 484
CviAII CATG 2 cut(s) 17, 630
CviQI GTAC 2 cut(s) 294, 484
DdeI CTNAG 2 cut(s) 1001, 1106
DpnI GATC 4 cut(s) 173, 521, 720, 965
DpnII GATC 4 cut(s) 171, 519, 718, 963
Eam1104I CTCTTC 2 cut(s) 155, 439
EarI CTCTTC 2 cut(s) 155, 439
Ecl136II GAGCTC 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 912
Eco24I GRGCYC 1 cut(s) 89
Eco32I GATATC 1 cut(s) 360
Eco47I GGWCC 2 cut(s) 909, 1056
Eco53kI GAGCTC 1 cut(s) 87
Eco57I CTGAAG 3 cut(s) 420, 432, 909
Eco81I CCTNAGG 1 cut(s) 1001
Eco88I CYCGRG 1 cut(s) 737
EcoICRI GAGCTC 1 cut(s) 87
EcoO109I RGGNCCY 2 cut(s) 762, 909
EcoRI GAATTC 1 cut(s) 405
EcoRII CCWGG 1 cut(s) 758
EcoRV GATATC 1 cut(s) 360
EcoT14I CCWWGG 1 cut(s) 912
EcoT38I GRGCYC 1 cut(s) 89
ErhI CCWWGG 1 cut(s) 912
Esp3I CGTCTC 1 cut(s) 1102
FaeI CATG 2 cut(s) 20, 633
FaqI GGGAC 2 cut(s) 640, 922
FatI CATG 2 cut(s) 16, 629
FauI CCCGC 2 cut(s) 391, 898
FbaI TGATCA 1 cut(s) 963
Fnu4HI GCNGC 5 cut(s) 263, 342, 543, 1080, 1114
FokI GGATG 5 cut(s) 206, 248, 269, 415, 622
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 5 cut(s) 263, 342, 543, 1080, 1114
FspBI CTAG 3 cut(s) 332, 902, 1126
GlaI GCGC 1 cut(s) 1010
GluI GCNGC 5 cut(s) 263, 342, 543, 1080, 1114
GsaI CCCAGC 1 cut(s) 503
GsuI CTGGAG 2 cut(s) 962, 963
HaeII RGCGCY 1 cut(s) 1012
HaeIII GGCC 1 cut(s) 764
HapII CCGG 1 cut(s) 512
HgaI GACGC 2 cut(s) 111, 1119
HhaI GCGC 1 cut(s) 1011
Hin1I GRCGYC 1 cut(s) 1111
Hin1II CATG 2 cut(s) 20, 633
Hin6I GCGC 1 cut(s) 1009
HinP1I GCGC 1 cut(s) 1009
HindIII AAGCTT 4 cut(s) 178, 474, 743, 807
HinfI GANTC 3 cut(s) 352, 647, 993
HpaII CCGG 1 cut(s) 512
HphI GGTGA 3 cut(s) 361, 457, 958
Hpy166II GTNNAC 2 cut(s) 380, 554
Hpy188I TCNGA 9 cut(s) 66, 101, 145, 176, 256, 357, 609, 769, 928
Hpy188III TCNNGA 7 cut(s) 169, 233, 419, 657, 739, 781, 1000
Hpy8I GTNNAC 2 cut(s) 380, 554
HpyAV CCTTC 1 cut(s) 980
HpyCH4III ACNGT 2 cut(s) 349, 1033
HpyCH4IV ACGT 1 cut(s) 774
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 320, 902, 1034, 1079, 1088
HpyF3I CTNAG 2 cut(s) 1001, 1106
HpySE526I ACGT 1 cut(s) 774
Hsp92I GRCGYC 1 cut(s) 1111
Hsp92II CATG 2 cut(s) 20, 633
HspAI GCGC 1 cut(s) 1009
Ksp22I TGATCA 1 cut(s) 963
Kzo9I GATC 4 cut(s) 171, 519, 718, 963
LmnI GCTCC 2 cut(s) 670, 1006
Lsp1109I GCAGC 3 cut(s) 249, 529, 1066
LweI GCATC 5 cut(s) 184, 378, 600, 720, 1091
MaeI CTAG 3 cut(s) 332, 902, 1126
MaeII ACGT 1 cut(s) 774
MaeIII GTNAC 1 cut(s) 349
MalI GATC 4 cut(s) 173, 521, 720, 965
MboI GATC 4 cut(s) 171, 519, 718, 963
MboII GAAGA 7 cut(s) 142, 254, 426, 581, 915, 988, 1054
MfeI CAATTG 2 cut(s) 324, 1044
MflI RGATCY 1 cut(s) 519
MhlI GDGCHC 2 cut(s) 89, 382
MluCI AATT 7 cut(s) 288, 301, 324, 405, 411, 684, 1044
MlyI GAGTC 1 cut(s) 346
MmeI TCCRAC 1 cut(s) 914
MseI TTAA 2 cut(s) 812, 986
MslI CAYNNNNRTG 1 cut(s) 514
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 2 cut(s) 513, 760
MunI CAATTG 2 cut(s) 324, 1044
Mva1269I GAATGC 1 cut(s) 134
MvaI CCWGG 1 cut(s) 760
MwoI GCNNNNNNNGC 6 cut(s) 11, 320, 902, 1034, 1079, 1088
NciI CCSGG 1 cut(s) 513
NdeII GATC 4 cut(s) 171, 519, 718, 963
NlaIII CATG 2 cut(s) 20, 633
NlaIV GGNNCC 1 cut(s) 910
NmuCI GTSAC 1 cut(s) 349
OliI CACNNNNGTG 1 cut(s) 514
PctI GAATGC 1 cut(s) 134
PfeI GAWTC 2 cut(s) 647, 993
PkrI GCNGC 5 cut(s) 264, 343, 544, 1081, 1115
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PpuMI RGGWCCY 1 cut(s) 909
Psp124BI GAGCTC 1 cut(s) 89
Psp5II RGGWCCY 1 cut(s) 909
Psp6I CCWGG 1 cut(s) 758
PspFI CCCAGC 1 cut(s) 499
PspGI CCWGG 1 cut(s) 758
PspN4I GGNNCC 1 cut(s) 910
PspPI GGNCC 3 cut(s) 762, 909, 1056
PspPPI RGGWCCY 1 cut(s) 909
PstI CTGCAG 2 cut(s) 547, 829
PsuI RGATCY 1 cut(s) 519
RsaI GTAC 2 cut(s) 295, 485
RsaNI GTAC 2 cut(s) 294, 484
RseI CAYNNNNRTG 1 cut(s) 514
SacI GAGCTC 1 cut(s) 89
SaqAI TTAA 2 cut(s) 812, 986
SatI GCNGC 5 cut(s) 263, 342, 543, 1080, 1114
Sau3AI GATC 4 cut(s) 171, 519, 718, 963
Sau96I GGNCC 3 cut(s) 762, 909, 1056
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 2 cut(s) 513, 760
SduI GDGCHC 2 cut(s) 89, 382
SfaNI GCATC 5 cut(s) 184, 378, 600, 720, 1091
SfcI CTRYAG 3 cut(s) 543, 825, 1074
SinI GGWCC 2 cut(s) 909, 1056
SmiMI CAYNNNNRTG 1 cut(s) 514
Sse9I AATT 7 cut(s) 288, 301, 324, 405, 411, 684, 1044
SsiI CCGC 6 cut(s) 5, 342, 384, 905, 957, 1114
SspI AATATT 1 cut(s) 583
SspMI CTAG 3 cut(s) 332, 902, 1126
SstI GAGCTC 1 cut(s) 89
StyD4I CCNGG 2 cut(s) 511, 758
StyI CCWWGG 1 cut(s) 912
TaaI ACNGT 2 cut(s) 349, 1033
TaiI ACGT 1 cut(s) 777
TaqI TCGA 4 cut(s) 83, 398, 656, 782
TasI AATT 7 cut(s) 288, 301, 324, 405, 411, 684, 1044
TatI WGTACW 1 cut(s) 293
TauI GCSGC 2 cut(s) 344, 1116
TfiI GAWTC 2 cut(s) 647, 993
Tru1I TTAA 2 cut(s) 812, 986
Tru9I TTAA 2 cut(s) 812, 986
TscAI CASTG 2 cut(s) 721, 834
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 3 cut(s) 262, 542, 1079
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 2 cut(s) 294, 417
TspGWI ACGGA 2 cut(s) 58, 131
TspRI CASTG 2 cut(s) 721, 834
VneI GTGCAC 1 cut(s) 378
VpaK11BI GGWCC 2 cut(s) 909, 1056
XapI RAATTY 3 cut(s) 301, 405, 411
XspI CTAG 3 cut(s) 332, 902, 1126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.