Rroxscaffold_7G00184350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004444
Physical Location & Seq
Reverse (-)
23565092 .. 23571081
5990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00184350.1

Sequence Viewer

Length: 1152 bp
ATGTCTCTAATAGCGGATTTTGCACCTGGGTCTTCTCTATTCCCACACACCCCAAAACCCAAATCTTCAAACCGACAACTTATTAGGCATTCAAGCTGTTTCAATGTCAAACACAGATTGCTGGTGAATGGGAAACAGTGTGGGCTAGTTTCCCAGCTCTTCAGACCCCAAAAGTTCAGGATTAGATGCTTTTCGAAGGAGGAGGATACAGAAAATGAAAGTGATGAGGCTGCAATGTCTAATGACATTCTGAATGGGCAAGATGGGATTGATGATGAGCTGCTTGGAAATGTTAAAGTTACTCAAACTACTACAGAAAGTTCATTTCTGGCTAAAATAGCAATTGCACTGGGAATAGCTGCAACAATTACATTGATATCTATTAGCACCAAGGGTCCCACTCTTGGATCATCGAATGGGCTTCAGTTCTTGCCAGAAAGCTCATCCTCTTCAGCAATGGCGGCGGCCCCAGTTGGTTTCACTTTCAAAGCTTTTGGCTACAGCATTGTTCTTCCAGAATATGCACCAGGGTGGACCTACTTTTGGTTGCTTATGGCTGCAGGGTTTGGGCTTTTCATCAGTGAAGAGGCTCTAAATATATGGGTTGGCATATCTTTGGCCCGGAGGCTATCTTTGGATGGGACCTGGCAGTCATTTGCTGAATCTTTCTCAAGAAATGCTCCGTACATTGTATCTACAACTCTTTGGGTGTATTGGGGAGTATGCATTAATGATCTAATACATTTTTATATGGGGAAGCTTTTTAGACAATCCAGGGCTTCTGATGATATTTTTTCAAAGTTAGGGATTGGCAAAGAGAAAGCTTTAAGCATAACCCGTGCAGTGCAAAGATATGGCAACCTCATTGGTTTTGTTGAACGCTTTTCTCTTGGAATGAGAAATCCAACAGCTTTTCTAGCAGGGACCTTAGGTATATCTCCAGAGTGTTTCTTTGCTGGAGTTTGTTTGGGTGGCTTGATCACCCTTCCAATCCAGTTCGGGATAGGATTCTTCTTGAGGGAGCGCCCTGTCTTTGCCCTGGCTACTGCTACAACAGTTGTGGGCATTTGGACTATTTTCCCTTATGCAGTGGCTGCATTAACAGCATTGTTCCTTTACCTTCGACAACGTTACTCAACGTTGTCAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

383

Amino Acids

42.02

Weight (kDa)

8.84

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 232 - 335 4.5e-07 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 649
AciI CCGC 3 cut(s) 14, 461, 464
AclI AACGTT 2 cut(s) 1129, 1139
AclWI GGATC 1 cut(s) 415
AcuI CTGAAG 3 cut(s) 145, 407, 435
AfaI GTAC 1 cut(s) 686
AfiI CCNNNNNNNGG 2 cut(s) 404, 543
AgsI TTSAA 6 cut(s) 69, 93, 103, 487, 798, 878
AjnI CCWGG 5 cut(s) 25, 526, 644, 773, 1038
AleI CACNNNNGTG 1 cut(s) 529
AluBI AGCT 9 cut(s) 96, 157, 280, 359, 441, 491, 760, 824, 911
AluI AGCT 9 cut(s) 96, 157, 280, 359, 441, 491, 760, 824, 911
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 415
AlwNI CAGNNNCTG 1 cut(s) 1094
AoxI GGCC 2 cut(s) 465, 618
ApeKI GCWGC 5 cut(s) 230, 280, 359, 557, 1094
AseI ATTAAT 1 cut(s) 729
AspLEI GCGC 1 cut(s) 1026
AspS9I GGNCC 6 cut(s) 395, 466, 534, 619, 642, 924
AsuC2I CCSGG 1 cut(s) 622
AsuHPI GGTGA 2 cut(s) 136, 973
AsuII TTCGAA 1 cut(s) 194
AvaII GGWCC 4 cut(s) 395, 534, 642, 924
AxyI CCTNAGG 1 cut(s) 928
BbsI GAAGAC 1 cut(s) 24
BbvI GCAGC 5 cut(s) 217, 267, 346, 544, 1081
BccI CCATC 2 cut(s) 257, 632
BciT130I CCWGG 5 cut(s) 27, 528, 646, 775, 1040
BciVI GTATCC 1 cut(s) 199
BclI TGATCA 1 cut(s) 978
BcnI CCSGG 1 cut(s) 622
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 2 cut(s) 146, 917
BfmI CTRYAG 3 cut(s) 312, 499, 558
BfoI RGCGCY 1 cut(s) 1027
BfuI GTATCC 1 cut(s) 199
BisI GCNGC 7 cut(s) 231, 281, 360, 462, 465, 558, 1095
BlsI GCNGC 7 cut(s) 232, 282, 361, 463, 466, 559, 1096
Bme1390I CCNGG 6 cut(s) 27, 528, 622, 646, 775, 1040
Bme18I GGWCC 4 cut(s) 395, 534, 642, 924
BmgT120I GGNCC 6 cut(s) 395, 466, 534, 619, 642, 924
BmiI GGNNCC 5 cut(s) 396, 397, 468, 643, 925
BmrFI CCNGG 6 cut(s) 27, 528, 622, 646, 775, 1040
BmrI ACTGGG 2 cut(s) 359, 464
BmsI GCATC 1 cut(s) 176
BmuI ACTGGG 2 cut(s) 359, 464
BpiI GAAGAC 1 cut(s) 24
BpmI CTGGAG 2 cut(s) 924, 978
Bpu14I TTCGAA 1 cut(s) 194
BpuEI CTTGAG 2 cut(s) 655, 1036
BpuMI CCSGG 1 cut(s) 622
BsaJI CCNNGG 5 cut(s) 26, 390, 527, 774, 1038
Bsc4I CCNNNNNNNGG 2 cut(s) 404, 543
Bse1I ACTGG 3 cut(s) 354, 470, 994
Bse21I CCTNAGG 1 cut(s) 928
Bse3DI GCAATG 2 cut(s) 240, 462
BseBI CCWGG 5 cut(s) 27, 528, 646, 775, 1040
BseDI CCNNGG 5 cut(s) 26, 390, 527, 774, 1038
BseGI GGATG 2 cut(s) 443, 643
BseLI CCNNNNNNNGG 2 cut(s) 404, 543
BseMI GCAATG 2 cut(s) 240, 462
BseNI ACTGG 3 cut(s) 354, 470, 994
BseRI GAGGAG 1 cut(s) 215
BseXI GCAGC 5 cut(s) 217, 267, 346, 544, 1081
BseYI CCCAGC 1 cut(s) 153
BsgI GTGCAG 1 cut(s) 861
BshFI GGCC 2 cut(s) 467, 620
BsiSI CCGG 1 cut(s) 622
BslFI GGGAC 3 cut(s) 381, 655, 937
BslI CCNNNNNNNGG 2 cut(s) 404, 543
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 3 cut(s) 381, 655, 937
BsmI GAATGC 1 cut(s) 88
BsnI GGCC 2 cut(s) 467, 620
Bsp119I TTCGAA 1 cut(s) 194
Bsp143I GATC 3 cut(s) 407, 733, 978
BspACI CCGC 3 cut(s) 14, 461, 464
BspANI GGCC 2 cut(s) 467, 620
BspLI GGNNCC 5 cut(s) 396, 397, 468, 643, 925
BspMAI CTGCAG 1 cut(s) 562
BspPI GGATC 1 cut(s) 415
BspQI GCTCTTC 1 cut(s) 164
BspT104I TTCGAA 1 cut(s) 194
BsrDI GCAATG 2 cut(s) 240, 462
BsrI ACTGG 3 cut(s) 354, 470, 994
BssECI CCNNGG 5 cut(s) 26, 390, 527, 774, 1038
BssMI GATC 3 cut(s) 407, 733, 978
BssT1I CCWWGG 1 cut(s) 390
Bst2UI CCWGG 5 cut(s) 27, 528, 646, 775, 1040
Bst4CI ACNGT 2 cut(s) 138, 1057
Bst6I CTCTTC 3 cut(s) 164, 454, 579
BstAPI GCANNNNNTGC 1 cut(s) 1094
BstBI TTCGAA 1 cut(s) 194
BstDEI CTNAG 1 cut(s) 928
BstF5I GGATG 2 cut(s) 443, 643
BstH2I RGCGCY 1 cut(s) 1027
BstHHI GCGC 1 cut(s) 1026
BstKTI GATC 3 cut(s) 410, 736, 981
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 3 cut(s) 407, 733, 978
BstMWI GCNNNNNNNGC 6 cut(s) 20, 338, 461, 917, 1094, 1103
BstNI CCWGG 5 cut(s) 27, 528, 646, 775, 1040
BstSCI CCNGG 6 cut(s) 25, 526, 620, 644, 773, 1038
BstSFI CTRYAG 3 cut(s) 312, 499, 558
BstV1I GCAGC 5 cut(s) 217, 267, 346, 544, 1081
BstV2I GAAGAC 1 cut(s) 24
Bsu36I CCTNAGG 1 cut(s) 928
BsuI GTATCC 1 cut(s) 199
BsuRI GGCC 2 cut(s) 467, 620
BtsCI GGATG 2 cut(s) 443, 643
BtsI GCAGTG 2 cut(s) 849, 1095
BtsIMutI CAGTG 5 cut(s) 143, 347, 586, 849, 1095
CaiI CAGNNNCTG 1 cut(s) 1094
CfoI GCGC 1 cut(s) 1026
Cfr13I GGNCC 6 cut(s) 395, 466, 534, 619, 642, 924
Csp6I GTAC 1 cut(s) 685
CspCI CAANNNNNGTGG 2 cut(s) 1041, 1076
CviQI GTAC 1 cut(s) 685
DdeI CTNAG 1 cut(s) 928
DpnI GATC 3 cut(s) 409, 735, 980
DpnII GATC 3 cut(s) 407, 733, 978
DrdI GACNNNNNNGTC 1 cut(s) 649
DseDI GACNNNNNNGTC 1 cut(s) 649
Eam1104I CTCTTC 3 cut(s) 164, 454, 579
EarI CTCTTC 3 cut(s) 164, 454, 579
Eco130I CCWWGG 1 cut(s) 390
Eco32I GATATC 1 cut(s) 378
Eco47I GGWCC 4 cut(s) 395, 534, 642, 924
Eco57I CTGAAG 3 cut(s) 145, 407, 435
Eco81I CCTNAGG 1 cut(s) 928
EcoO109I RGGNCCY 3 cut(s) 395, 642, 924
EcoRII CCWGG 5 cut(s) 25, 526, 644, 773, 1038
EcoRV GATATC 1 cut(s) 378
EcoT14I CCWWGG 1 cut(s) 390
EcoT22I ATGCAT 1 cut(s) 728
ErhI CCWWGG 1 cut(s) 390
FaqI GGGAC 3 cut(s) 381, 655, 937
FbaI TGATCA 1 cut(s) 978
Fnu4HI GCNGC 7 cut(s) 231, 281, 360, 462, 465, 558, 1095
FokI GGATG 2 cut(s) 430, 650
Fsp4HI GCNGC 7 cut(s) 231, 281, 360, 462, 465, 558, 1095
FspBI CTAG 2 cut(s) 146, 917
GlaI GCGC 1 cut(s) 1025
GluI GCNGC 7 cut(s) 231, 281, 360, 462, 465, 558, 1095
GsaI CCCAGC 1 cut(s) 157
GsuI CTGGAG 2 cut(s) 924, 978
HaeII RGCGCY 1 cut(s) 1027
HaeIII GGCC 2 cut(s) 467, 620
HapII CCGG 1 cut(s) 622
HhaI GCGC 1 cut(s) 1026
Hin6I GCGC 1 cut(s) 1024
HinP1I GCGC 1 cut(s) 1024
HindIII AAGCTT 3 cut(s) 489, 758, 822
HinfI GANTC 2 cut(s) 662, 1008
HpaII CCGG 1 cut(s) 622
HphI GGTGA 2 cut(s) 136, 973
Hpy166II GTNNAC 1 cut(s) 534
Hpy188I TCNGA 4 cut(s) 164, 252, 784, 1147
Hpy188III TCNNGA 6 cut(s) 178, 515, 672, 941, 1000, 1015
Hpy8I GTNNAC 1 cut(s) 534
HpyAV CCTTC 3 cut(s) 190, 995, 1130
HpyCH4III ACNGT 2 cut(s) 138, 1057
HpyCH4IV ACGT 2 cut(s) 1129, 1139
HpyF10VI GCNNNNNNNGC 6 cut(s) 20, 338, 461, 917, 1094, 1103
HpyF3I CTNAG 1 cut(s) 928
HpySE526I ACGT 2 cut(s) 1129, 1139
HspAI GCGC 1 cut(s) 1024
KflI GGGWCCC 1 cut(s) 395
Ksp22I TGATCA 1 cut(s) 978
Kzo9I GATC 3 cut(s) 407, 733, 978
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 2 cut(s) 685, 1021
Lsp1109I GCAGC 5 cut(s) 217, 267, 346, 544, 1081
LweI GCATC 1 cut(s) 176
MaeI CTAG 2 cut(s) 146, 917
MaeII ACGT 2 cut(s) 1129, 1139
MaeIII GTNAC 2 cut(s) 298, 1130
MalI GATC 3 cut(s) 409, 735, 980
MboI GATC 3 cut(s) 407, 733, 978
MboII GAAGA 7 cut(s) 24, 57, 151, 441, 503, 596, 1003
MfeI CAATTG 1 cut(s) 342
MluCI AATT 2 cut(s) 342, 366
MmeI TCCRAC 1 cut(s) 929
MnlI CCTC 8 cut(s) 193, 196, 220, 457, 580, 618, 872, 1011
Mph1103I ATGCAT 1 cut(s) 728
MseI TTAA 4 cut(s) 294, 729, 827, 1100
MslI CAYNNNNRTG 1 cut(s) 529
MspI CCGG 1 cut(s) 622
MspR9I CCNGG 6 cut(s) 27, 528, 622, 646, 775, 1040
MunI CAATTG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 88
MvaI CCWGG 5 cut(s) 27, 528, 646, 775, 1040
MwoI GCNNNNNNNGC 6 cut(s) 20, 338, 461, 917, 1094, 1103
NciI CCSGG 1 cut(s) 622
NdeII GATC 3 cut(s) 407, 733, 978
NlaIV GGNNCC 5 cut(s) 396, 397, 468, 643, 925
NsiI ATGCAT 1 cut(s) 728
NspV TTCGAA 1 cut(s) 194
OliI CACNNNNGTG 1 cut(s) 529
PciSI GCTCTTC 1 cut(s) 164
PctI GAATGC 1 cut(s) 88
PfeI GAWTC 2 cut(s) 662, 1008
PkrI GCNGC 7 cut(s) 232, 282, 361, 463, 466, 559, 1096
PpuMI RGGWCCY 3 cut(s) 395, 642, 924
PshBI ATTAAT 1 cut(s) 729
Psp1406I AACGTT 2 cut(s) 1129, 1139
Psp5II RGGWCCY 3 cut(s) 395, 642, 924
Psp6I CCWGG 5 cut(s) 25, 526, 644, 773, 1038
PspFI CCCAGC 1 cut(s) 153
PspGI CCWGG 5 cut(s) 25, 526, 644, 773, 1038
PspN4I GGNNCC 5 cut(s) 396, 397, 468, 643, 925
PspPI GGNCC 6 cut(s) 395, 466, 534, 619, 642, 924
PspPPI RGGWCCY 3 cut(s) 395, 642, 924
PstI CTGCAG 1 cut(s) 562
PstNI CAGNNNCTG 1 cut(s) 1094
RsaI GTAC 1 cut(s) 686
RsaNI GTAC 1 cut(s) 685
RseI CAYNNNNRTG 1 cut(s) 529
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 4 cut(s) 294, 729, 827, 1100
SatI GCNGC 7 cut(s) 231, 281, 360, 462, 465, 558, 1095
Sau3AI GATC 3 cut(s) 407, 733, 978
Sau96I GGNCC 6 cut(s) 395, 466, 534, 619, 642, 924
ScrFI CCNGG 6 cut(s) 27, 528, 622, 646, 775, 1040
SfaNI GCATC 1 cut(s) 176
SfcI CTRYAG 3 cut(s) 312, 499, 558
SfuI TTCGAA 1 cut(s) 194
SinI GGWCC 4 cut(s) 395, 534, 642, 924
SmiMI CAYNNNNRTG 1 cut(s) 529
SmlI CTYRAG 2 cut(s) 670, 1015
SmoI CTYRAG 2 cut(s) 670, 1015
Sse9I AATT 2 cut(s) 342, 366
SsiI CCGC 3 cut(s) 14, 461, 464
SspMI CTAG 2 cut(s) 146, 917
StyD4I CCNGG 6 cut(s) 25, 526, 620, 644, 773, 1038
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 2 cut(s) 138, 1057
TaiI ACGT 2 cut(s) 1132, 1142
TaqI TCGA 3 cut(s) 194, 413, 1123
TasI AATT 2 cut(s) 342, 366
TauI GCSGC 2 cut(s) 464, 467
TfiI GAWTC 2 cut(s) 662, 1008
Tru1I TTAA 4 cut(s) 294, 729, 827, 1100
Tru9I TTAA 4 cut(s) 294, 729, 827, 1100
TscAI CASTG 5 cut(s) 143, 354, 586, 849, 1095
TseI GCWGC 5 cut(s) 230, 280, 359, 557, 1094
TspDTI ATGAA 3 cut(s) 231, 312, 565
TspGWI ACGGA 1 cut(s) 672
TspRI CASTG 5 cut(s) 143, 354, 586, 849, 1095
VpaK11BI GGWCC 4 cut(s) 395, 534, 642, 924
VspI ATTAAT 1 cut(s) 729
XspI CTAG 2 cut(s) 146, 917
Zsp2I ATGCAT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.