pycom05g16170

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004444
Physical Location & Seq
Forward (+)
19521116 .. 19524064
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g16170.10

Sequence Viewer

Length: 1128 bp
ATGGCGATTTTGGCCCATGGGTTTTATCTACCCCCACCCACACGGACACCCAAATCATCAAATCGGAAACTTGTTGGGTATTCGAGCTCTATCATTGTCAGACGCAGATTGCTGACCGACGGGAACGAATGCGTGCAGGTTTCAGAACTCTTCACGCCCAAGACTTTCAGGATCAGAAGCTTTTCTGAGGAGGATGCAGGACATAATGAGGTTGAAATATCTAAAGACATTCTGGTTGGGGAAGATGGGATTTCTGATGAGCTGCTTGGAAATGTTATGAATGCTGAAATTAGTACGGAAAGTTCATTTCTTGCTAAAATAGCAATTGCACTAGGCGTTGCCGCAACAGTGACTCTGATATCGGTTGGCATCAAGGGTGCACCCGCAATTGGGTCATCGAATGGAATTCAAATTTTTCCAGATAGTTCATCCTCTTCAGTAATGGCTGCAGCCAATGTCGGTTTCACTATCAAAGCTTTTGGGTACAGCATTGTTCTCCCAGCATTTGCACCGGGGTGGATCTACTTTTGGTTGCTTATGGCTGCAGGCTGTGGACTTTTCATTAGTGAAGAGGCTCTAAATATTTGGGTTGGCATATCTTTGGCTCAGATGCTGTCTTTGGATGGGACATGTCAGTCGTTCGCTGAATCTTTTTCGAGAAATGCTCCATATATCATATCCACAATTCTTTGGGTGTATTGGGGAGTGTGCATCAGTGATCTAATACCATTTTATCTCGGGAAGCTTTTTAGAAAATCCAGGGCCTCCAATGATGTTCTCTCGAAGCTAGGGATTGGAAAAGAGAAAGCTTTAAGCATAACCTCTGCAGTGCAAAAATATGGCAACCTCATTGGTTTCGTTGAACGATTTTCGCTTGGAGTGAGAAATCCAACGGCTTTTCTAGCAGGGACCTTGGGCATTTCTTCAGAGTGTTTCTTTGCTGGAGTTTGTTGTGGCGGTTTGATCACCCTGCCTCTCCAGTTGTTAATAGGATTCTTCCTAAGGGAGCACCCCGTCTTTGCCCTCACTACTGTTGCAACAACAATTGGCATTTGGACCATCTTCCCTTACGCCGTGGCTGCATCAACGGCATTATTCCTTTACTTGAGACGCCACTACTCTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

376

Amino Acids

40.43

Weight (kDa)

8.11

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 634
Acc36I ACCTGC 1 cut(s) 127
AciI CCGC 3 cut(s) 342, 384, 957
AclWI GGATC 2 cut(s) 179, 527
AcsI RAATTY 2 cut(s) 405, 411
AcuI CTGAAG 2 cut(s) 420, 909
AcyI GRCGYC 1 cut(s) 1111
AfaI GTAC 2 cut(s) 295, 485
AfiI CCNNNNNNNGG 2 cut(s) 389, 390
AflIII ACRYGT 1 cut(s) 629
AgsI TTSAA 3 cut(s) 215, 410, 863
AjnI CCWGG 1 cut(s) 758
AjuI GAANNNNNNNTTGG 2 cut(s) 446, 478
AleI CACNNNNGTG 1 cut(s) 514
AluBI AGCT 7 cut(s) 87, 180, 262, 476, 745, 787, 809
AluI AGCT 7 cut(s) 87, 180, 262, 476, 745, 787, 809
Alw21I GWGCWC 3 cut(s) 89, 382, 1011
Alw26I GTCTC 1 cut(s) 1102
Alw44I GTGCAC 1 cut(s) 378
AlwI GGATC 2 cut(s) 179, 527
AlwNI CAGNNNCTG 1 cut(s) 613
Ama87I CYCGRG 1 cut(s) 737
AoxI GGCC 2 cut(s) 12, 762
ApaLI GTGCAC 1 cut(s) 378
ApeKI GCWGC 5 cut(s) 262, 446, 449, 542, 1079
ApoI RAATTY 2 cut(s) 405, 411
Asp700I GAANNNNTTC 1 cut(s) 181
AspS9I GGNCC 4 cut(s) 13, 762, 909, 1056
AsuC2I CCSGG 1 cut(s) 513
AsuHPI GGTGA 1 cut(s) 958
AvaI CYCGRG 1 cut(s) 737
AvaII GGWCC 2 cut(s) 909, 1056
AxyI CCTNAGG 1 cut(s) 1001
BaeGI GKGCMC 1 cut(s) 382
BanII GRGCYC 1 cut(s) 89
Bbv12I GWGCWC 3 cut(s) 89, 382, 1011
BbvI GCAGC 5 cut(s) 249, 433, 461, 529, 1066
BccI CCATC 3 cut(s) 239, 617, 1067
BceAI ACGGC 3 cut(s) 909, 1058, 1104
BciT130I CCWGG 1 cut(s) 760
BclI TGATCA 1 cut(s) 963
BcnI CCSGG 1 cut(s) 513
BcoDI GTCTC 1 cut(s) 1102
BfaI CTAG 4 cut(s) 332, 788, 902, 1126
BfmI CTRYAG 3 cut(s) 447, 543, 825
BfuAI ACCTGC 1 cut(s) 127
BisI GCNGC 6 cut(s) 263, 342, 447, 450, 543, 1080
BlsI GCNGC 6 cut(s) 264, 343, 448, 451, 544, 1081
Bme1390I CCNGG 2 cut(s) 513, 760
Bme18I GGWCC 2 cut(s) 909, 1056
BmeT110I CYCGRG 1 cut(s) 737
BmgT120I GGNCC 4 cut(s) 13, 762, 909, 1056
BmiI GGNNCC 1 cut(s) 910
BmrFI CCNGG 2 cut(s) 513, 760
BmsI GCATC 5 cut(s) 184, 378, 600, 720, 1091
BplI GAGNNNNNCTC 2 cut(s) 649, 681
BpmI CTGGAG 2 cut(s) 962, 963
BpuEI CTTGAG 1 cut(s) 1126
BpuMI CCSGG 1 cut(s) 513
BsaHI GRCGYC 1 cut(s) 1111
BsaJI CCNNGG 5 cut(s) 16, 512, 759, 912, 1074
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 390
Bse1I ACTGG 1 cut(s) 979
Bse21I CCTNAGG 1 cut(s) 1001
BseBI CCWGG 1 cut(s) 760
BseDI CCNNGG 5 cut(s) 16, 512, 759, 912, 1074
BseGI GGATG 3 cut(s) 199, 428, 628
BseLI CCNNNNNNNGG 2 cut(s) 389, 390
BseMII CTCAG 2 cut(s) 177, 620
BseNI ACTGG 1 cut(s) 979
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 5 cut(s) 249, 433, 461, 529, 1066
BseYI CCCAGC 1 cut(s) 499
BsgI GTGCAG 1 cut(s) 155
BshFI GGCC 2 cut(s) 14, 764
BsiHKAI GWGCWC 3 cut(s) 89, 382, 1011
BsiHKCI CYCGRG 1 cut(s) 737
BsiSI CCGG 1 cut(s) 512
BslFI GGGAC 2 cut(s) 640, 922
BslI CCNNNNNNNGG 2 cut(s) 389, 390
BsmAI GTCTC 1 cut(s) 1102
BsmBI CGTCTC 1 cut(s) 1102
BsmFI GGGAC 2 cut(s) 640, 922
BsmI GAATGC 2 cut(s) 134, 286
BsnI GGCC 2 cut(s) 14, 764
BsoBI CYCGRG 1 cut(s) 737
Bsp1286I GDGCHC 3 cut(s) 89, 382, 1011
Bsp143I GATC 4 cut(s) 171, 519, 718, 963
Bsp19I CCATGG 1 cut(s) 16
BspACI CCGC 3 cut(s) 342, 384, 957
BspANI GGCC 2 cut(s) 14, 764
BspCNI CTCAG 2 cut(s) 178, 619
BspLI GGNNCC 1 cut(s) 910
BspMAI CTGCAG 3 cut(s) 451, 547, 829
BspMI ACCTGC 1 cut(s) 127
BspPI GGATC 2 cut(s) 179, 527
BsrI ACTGG 1 cut(s) 979
BssECI CCNNGG 5 cut(s) 16, 512, 759, 912, 1074
BssMI GATC 4 cut(s) 171, 519, 718, 963
BssNI GRCGYC 1 cut(s) 1111
BssT1I CCWWGG 2 cut(s) 16, 912
Bst2UI CCWGG 1 cut(s) 760
Bst4CI ACNGT 2 cut(s) 349, 1033
Bst6I CTCTTC 3 cut(s) 155, 439, 564
BstACI GRCGYC 1 cut(s) 1111
BstC8I GCNNGC 2 cut(s) 134, 547
BstDEI CTNAG 3 cut(s) 186, 606, 1001
BstDSI CCRYGG 2 cut(s) 16, 1074
BstF5I GGATG 3 cut(s) 199, 428, 628
BstKTI GATC 4 cut(s) 174, 522, 721, 966
BstMAI GTCTC 1 cut(s) 1102
BstMBI GATC 4 cut(s) 171, 519, 718, 963
BstMWI GCNNNNNNNGC 5 cut(s) 11, 320, 902, 1079, 1088
BstNI CCWGG 1 cut(s) 760
BstNSI RCATGY 1 cut(s) 633
BstSCI CCNGG 2 cut(s) 511, 758
BstSFI CTRYAG 3 cut(s) 447, 543, 825
BstSLI GKGCMC 1 cut(s) 382
BstV1I GCAGC 5 cut(s) 249, 433, 461, 529, 1066
BstX2I RGATCY 1 cut(s) 519
BstYI RGATCY 1 cut(s) 519
Bsu36I CCTNAGG 1 cut(s) 1001
BsuRI GGCC 2 cut(s) 14, 764
BtgI CCRYGG 2 cut(s) 16, 1074
BtsCI GGATG 3 cut(s) 199, 428, 628
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 3 cut(s) 354, 721, 834
BveI ACCTGC 1 cut(s) 127
Cac8I GCNNGC 2 cut(s) 134, 547
CaiI CAGNNNCTG 1 cut(s) 613
Cfr13I GGNCC 4 cut(s) 13, 762, 909, 1056
CseI GACGC 2 cut(s) 111, 1119
Csp6I GTAC 2 cut(s) 294, 484
CviAII CATG 2 cut(s) 17, 630
CviQI GTAC 2 cut(s) 294, 484
DdeI CTNAG 3 cut(s) 186, 606, 1001
DpnI GATC 4 cut(s) 173, 521, 720, 965
DpnII GATC 4 cut(s) 171, 519, 718, 963
DrdI GACNNNNNNGTC 1 cut(s) 634
DseDI GACNNNNNNGTC 1 cut(s) 634
Eam1104I CTCTTC 3 cut(s) 155, 439, 564
EarI CTCTTC 3 cut(s) 155, 439, 564
Ecl136II GAGCTC 1 cut(s) 87
Eco130I CCWWGG 2 cut(s) 16, 912
Eco24I GRGCYC 1 cut(s) 89
Eco32I GATATC 1 cut(s) 360
Eco47I GGWCC 2 cut(s) 909, 1056
Eco53kI GAGCTC 1 cut(s) 87
Eco57I CTGAAG 2 cut(s) 420, 909
Eco81I CCTNAGG 1 cut(s) 1001
Eco88I CYCGRG 1 cut(s) 737
EcoICRI GAGCTC 1 cut(s) 87
EcoO109I RGGNCCY 2 cut(s) 762, 909
EcoRI GAATTC 1 cut(s) 405
EcoRII CCWGG 1 cut(s) 758
EcoRV GATATC 1 cut(s) 360
EcoT14I CCWWGG 2 cut(s) 16, 912
EcoT38I GRGCYC 1 cut(s) 89
ErhI CCWWGG 2 cut(s) 16, 912
Esp3I CGTCTC 1 cut(s) 1102
FaeI CATG 2 cut(s) 20, 633
FaqI GGGAC 2 cut(s) 640, 922
FatI CATG 2 cut(s) 16, 629
FauI CCCGC 1 cut(s) 391
FbaI TGATCA 1 cut(s) 963
Fnu4HI GCNGC 6 cut(s) 263, 342, 447, 450, 543, 1080
FokI GGATG 3 cut(s) 206, 415, 635
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 6 cut(s) 263, 342, 447, 450, 543, 1080
FspBI CTAG 4 cut(s) 332, 788, 902, 1126
GluI GCNGC 6 cut(s) 263, 342, 447, 450, 543, 1080
GsaI CCCAGC 1 cut(s) 503
GsuI CTGGAG 2 cut(s) 962, 963
HaeIII GGCC 2 cut(s) 14, 764
HapII CCGG 1 cut(s) 512
HgaI GACGC 2 cut(s) 111, 1119
Hin1I GRCGYC 1 cut(s) 1111
Hin1II CATG 2 cut(s) 20, 633
HindIII AAGCTT 4 cut(s) 178, 474, 743, 807
HinfI GANTC 3 cut(s) 352, 647, 993
HpaII CCGG 1 cut(s) 512
HphI GGTGA 1 cut(s) 958
Hpy166II GTNNAC 2 cut(s) 380, 554
Hpy188I TCNGA 9 cut(s) 66, 101, 145, 176, 187, 256, 357, 609, 928
Hpy188III TCNNGA 5 cut(s) 169, 419, 657, 739, 781
Hpy8I GTNNAC 2 cut(s) 380, 554
Hpy99I CGWCG 1 cut(s) 122
HpyCH4III ACNGT 2 cut(s) 349, 1033
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 320, 902, 1079, 1088
HpyF3I CTNAG 3 cut(s) 186, 606, 1001
Hsp92I GRCGYC 1 cut(s) 1111
Hsp92II CATG 2 cut(s) 20, 633
Ksp22I TGATCA 1 cut(s) 963
Kzo9I GATC 4 cut(s) 171, 519, 718, 963
LmnI GCTCC 2 cut(s) 670, 1006
Lsp1109I GCAGC 5 cut(s) 249, 433, 461, 529, 1066
LweI GCATC 5 cut(s) 184, 378, 600, 720, 1091
MaeI CTAG 4 cut(s) 332, 788, 902, 1126
MaeIII GTNAC 1 cut(s) 349
MalI GATC 4 cut(s) 173, 521, 720, 965
MboI GATC 4 cut(s) 171, 519, 718, 963
MboII GAAGA 7 cut(s) 142, 254, 426, 581, 915, 988, 1054
MfeI CAATTG 3 cut(s) 324, 387, 1044
MflI RGATCY 1 cut(s) 519
MhlI GDGCHC 3 cut(s) 89, 382, 1011
MluCI AATT 7 cut(s) 288, 324, 387, 405, 411, 684, 1044
MlyI GAGTC 1 cut(s) 346
MmeI TCCRAC 1 cut(s) 914
MroXI GAANNNNTTC 1 cut(s) 181
MseI TTAA 2 cut(s) 812, 986
MslI CAYNNNNRTG 1 cut(s) 514
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 2 cut(s) 513, 760
MunI CAATTG 3 cut(s) 324, 387, 1044
Mva1269I GAATGC 2 cut(s) 134, 286
MvaI CCWGG 1 cut(s) 760
MwoI GCNNNNNNNGC 5 cut(s) 11, 320, 902, 1079, 1088
NciI CCSGG 1 cut(s) 513
NcoI CCATGG 1 cut(s) 16
NdeII GATC 4 cut(s) 171, 519, 718, 963
NlaIII CATG 2 cut(s) 20, 633
NlaIV GGNNCC 1 cut(s) 910
NmuCI GTSAC 1 cut(s) 349
NspI RCATGY 1 cut(s) 633
OliI CACNNNNGTG 1 cut(s) 514
PciI ACATGT 1 cut(s) 629
PctI GAATGC 2 cut(s) 134, 286
PdmI GAANNNNTTC 1 cut(s) 181
PfeI GAWTC 2 cut(s) 647, 993
PkrI GCNGC 6 cut(s) 264, 343, 448, 451, 544, 1081
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PpuMI RGGWCCY 1 cut(s) 909
PscI ACATGT 1 cut(s) 629
Psp124BI GAGCTC 1 cut(s) 89
Psp5II RGGWCCY 1 cut(s) 909
Psp6I CCWGG 1 cut(s) 758
PspFI CCCAGC 1 cut(s) 499
PspGI CCWGG 1 cut(s) 758
PspN4I GGNNCC 1 cut(s) 910
PspPI GGNCC 4 cut(s) 13, 762, 909, 1056
PspPPI RGGWCCY 1 cut(s) 909
PsrI GAACNNNNNNTAC 2 cut(s) 286, 318
PstI CTGCAG 3 cut(s) 451, 547, 829
PstNI CAGNNNCTG 1 cut(s) 613
PsuI RGATCY 1 cut(s) 519
RsaI GTAC 2 cut(s) 295, 485
RsaNI GTAC 2 cut(s) 294, 484
RseI CAYNNNNRTG 1 cut(s) 514
SacI GAGCTC 1 cut(s) 89
SaqAI TTAA 2 cut(s) 812, 986
SatI GCNGC 6 cut(s) 263, 342, 447, 450, 543, 1080
Sau3AI GATC 4 cut(s) 171, 519, 718, 963
Sau96I GGNCC 4 cut(s) 13, 762, 909, 1056
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 2 cut(s) 513, 760
SduI GDGCHC 3 cut(s) 89, 382, 1011
SfaNI GCATC 5 cut(s) 184, 378, 600, 720, 1091
SfcI CTRYAG 3 cut(s) 447, 543, 825
SinI GGWCC 2 cut(s) 909, 1056
SmiMI CAYNNNNRTG 1 cut(s) 514
SmlI CTYRAG 1 cut(s) 1105
SmoI CTYRAG 1 cut(s) 1105
Sse9I AATT 7 cut(s) 288, 324, 387, 405, 411, 684, 1044
SsiI CCGC 3 cut(s) 342, 384, 957
SspI AATATT 1 cut(s) 583
SspMI CTAG 4 cut(s) 332, 788, 902, 1126
SstI GAGCTC 1 cut(s) 89
StyD4I CCNGG 2 cut(s) 511, 758
StyI CCWWGG 2 cut(s) 16, 912
TaaI ACNGT 2 cut(s) 349, 1033
TaqI TCGA 4 cut(s) 83, 398, 656, 782
TaqII GACCGA 1 cut(s) 131
TasI AATT 7 cut(s) 288, 324, 387, 405, 411, 684, 1044
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 2 cut(s) 647, 993
Tru1I TTAA 2 cut(s) 812, 986
Tru9I TTAA 2 cut(s) 812, 986
TscAI CASTG 3 cut(s) 354, 721, 834
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 5 cut(s) 262, 446, 449, 542, 1079
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 4 cut(s) 293, 294, 417, 550
TspGWI ACGGA 2 cut(s) 58, 311
TspRI CASTG 3 cut(s) 354, 721, 834
VneI GTGCAC 1 cut(s) 378
VpaK11BI GGWCC 2 cut(s) 909, 1056
XapI RAATTY 2 cut(s) 405, 411
XceI RCATGY 1 cut(s) 633
XmnI GAANNNNTTC 1 cut(s) 181
XspI CTAG 4 cut(s) 332, 788, 902, 1126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.