FvH4_2g21680

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006668
Physical Location & Seq
Forward (+)
17967418 .. 17970513
3096 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21680.t1

Sequence Viewer

Length: 822 bp
ATGAACTCTGCTCACCTCTTACACCTCTCTCCCCAATTCCATGCCCTAAATCCCAACCACTCTTCTTCTTCTTCTTCGATCAATCTCCTCCGACTCTGCAAATTCAACTCCGTTTCCCTCGCCTCCCTTCCCAAGCCCTCCCGCTTAATCACGCTTTGCGCCTCCAGTTCCGACAAGCTGTCGTCGGAATCGGCGCCGCCGGAGTCCATTAGGGAAGGGTTTCTTCATTTTCAGAACGACCCTTCGCAGTGGAATGTCGAAGTCGGAAGCCCTAAGGTTCCGGGTCCGTCCGTGGCCAAGTTGAGCTTGGGCGACCAGGCTTTCTTTCTCATGGCCTTCATTGCCTGCACGACTTCGGTGGCATTTACGAGCCTTGTAATTGCAGCTGTTCCGGCGATATGTGCAATGGGGAGAGCTGCAACATCTCTTTCAAAGCTGGCAGATACAGCTCGTCAAGAACTACCTAGTACTATGGCTGCCATTAGGCTCTCAGGCATGGAAATAAGTGATCTCACACTGGAACTCAATGACCTAAGCCAAGAGATAGCTGATGGGGTTAGCAAATCCACTCAAGCTGTTCAAGCAGCAGAAGCTGGGATTCGACAGATTGGCTCACTTGCTCGGCAGCAGACTATGTCAATGATTCAAGAGAGGGCAAACCTGCCGGTTATCTCTTTGCAACCTGCCGTTATTGGAGCAGCAAAAAAGACTTCTCAAGCTGTTGGCCAAGTGACTAAGAAGTTCATGAATATAATCTCTCAAAGGGACTCAGAGAATGAGGATGACATTGGAATTGAATCAATTGATAGGGTGGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

274

Amino Acids

29.05

Weight (kDa)

5.97

Isoelectric Point (pI)

50.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 669, 691
AccB1I GGYRCC 1 cut(s) 193
AciI CCGC 2 cut(s) 142, 197
AcoI YGGCCR 2 cut(s) 294, 724
AcsI RAATTY 2 cut(s) 101, 816
AcyI GRCGYC 1 cut(s) 194
AfaI GTAC 1 cut(s) 469
AgsI TTSAA 5 cut(s) 106, 432, 581, 647, 797
AhdI GACNNNNNGTC 1 cut(s) 178
AjnI CCWGG 1 cut(s) 315
AlwNI CAGNNNCTG 1 cut(s) 593
AoxI GGCC 3 cut(s) 294, 333, 724
ApeKI GCWGC 6 cut(s) 383, 416, 476, 584, 625, 698
ApoI RAATTY 2 cut(s) 101, 816
Asp700I GAANNNNTTC 1 cut(s) 219
AspLEI GCGC 2 cut(s) 161, 196
AspS9I GGNCC 1 cut(s) 284
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 1 cut(s) 5
AvaII GGWCC 1 cut(s) 284
AxyI CCTNAGG 1 cut(s) 273
BalI TGGCCA 2 cut(s) 296, 726
BanI GGYRCC 1 cut(s) 193
BbvI GCAGC 6 cut(s) 395, 403, 463, 596, 637, 710
BccI CCATC 1 cut(s) 545
BceAI ACGGC 1 cut(s) 671
BciT130I CCWGG 1 cut(s) 317
BcnI CCSGG 1 cut(s) 282
BfaI CTAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 197
BfuAI ACCTGC 2 cut(s) 669, 691
BisI GCNGC 7 cut(s) 197, 384, 417, 477, 585, 626, 699
BlsI GCNGC 7 cut(s) 198, 385, 418, 478, 586, 627, 700
BmcAI AGTACT 1 cut(s) 469
Bme1390I CCNGG 2 cut(s) 282, 317
Bme18I GGWCC 1 cut(s) 284
BmeRI GACNNNNNGTC 1 cut(s) 178
BmgT120I GGNCC 1 cut(s) 284
BmiI GGNNCC 3 cut(s) 195, 279, 285
BmrFI CCNGG 2 cut(s) 282, 317
BpmI CTGGAG 1 cut(s) 148
Bpu10I CCTNAGC 1 cut(s) 533
BpuEI CTTGAG 2 cut(s) 555, 699
BpuMI CCSGG 1 cut(s) 282
BsaHI GRCGYC 1 cut(s) 194
BsaJI CCNNGG 1 cut(s) 291
Bse118I RCCGGY 1 cut(s) 664
Bse1I ACTGG 2 cut(s) 165, 522
Bse21I CCTNAGG 1 cut(s) 273
Bse3DI GCAATG 2 cut(s) 339, 411
BseBI CCWGG 1 cut(s) 317
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 1 cut(s) 787
BseMI GCAATG 2 cut(s) 339, 411
BseMII CTCAG 2 cut(s) 504, 783
BseNI ACTGG 2 cut(s) 165, 522
BseRI GAGGAG 1 cut(s) 77
BseXI GCAGC 6 cut(s) 395, 403, 463, 596, 637, 710
BseYI CCCAGC 1 cut(s) 593
BsgI GTGCAG 1 cut(s) 331
BshFI GGCC 3 cut(s) 296, 335, 726
BshNI GGYRCC 1 cut(s) 193
BsiSI CCGG 4 cut(s) 200, 281, 392, 665
BslFI GGGAC 1 cut(s) 779
BsmFI GGGAC 1 cut(s) 779
BsnI GGCC 3 cut(s) 296, 335, 726
Bsp143I GATC 2 cut(s) 78, 508
BspACI CCGC 2 cut(s) 142, 197
BspANI GGCC 3 cut(s) 296, 335, 726
BspCNI CTCAG 2 cut(s) 503, 782
BspHI TCATGA 1 cut(s) 744
BspLI GGNNCC 3 cut(s) 195, 279, 285
BspMI ACCTGC 2 cut(s) 669, 691
BspT107I GGYRCC 1 cut(s) 193
BsrDI GCAATG 2 cut(s) 339, 411
BsrFI RCCGGY 1 cut(s) 664
BsrI ACTGG 2 cut(s) 165, 522
BssAI RCCGGY 1 cut(s) 664
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 2 cut(s) 78, 508
BssNI GRCGYC 1 cut(s) 194
Bst2UI CCWGG 1 cut(s) 317
Bst6I CTCTTC 1 cut(s) 67
BstACI GRCGYC 1 cut(s) 194
BstC8I GCNNGC 2 cut(s) 346, 438
BstDEI CTNAG 5 cut(s) 273, 490, 533, 735, 769
BstDSI CCRYGG 1 cut(s) 291
BstF5I GGATG 1 cut(s) 787
BstH2I RGCGCY 1 cut(s) 197
BstHHI GCGC 2 cut(s) 161, 196
BstKTI GATC 2 cut(s) 81, 511
BstMBI GATC 2 cut(s) 78, 508
BstMWI GCNNNNNNNGC 6 cut(s) 341, 392, 401, 446, 581, 590
BstNI CCWGG 1 cut(s) 317
BstSCI CCNGG 2 cut(s) 280, 315
BstV1I GCAGC 6 cut(s) 395, 403, 463, 596, 637, 710
Bsu36I CCTNAGG 1 cut(s) 273
BsuRI GGCC 3 cut(s) 296, 335, 726
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 1 cut(s) 787
BtsI GCAGTG 1 cut(s) 254
BtsIMutI CAGTG 2 cut(s) 254, 515
BveI ACCTGC 2 cut(s) 669, 691
Cac8I GCNNGC 2 cut(s) 346, 438
CaiI CAGNNNCTG 1 cut(s) 593
CciI TCATGA 1 cut(s) 744
CfoI GCGC 2 cut(s) 161, 196
Cfr10I RCCGGY 1 cut(s) 664
Cfr13I GGNCC 1 cut(s) 284
Csp6I GTAC 1 cut(s) 468
CviAII CATG 4 cut(s) 41, 331, 496, 745
CviQI GTAC 1 cut(s) 468
DdeI CTNAG 5 cut(s) 273, 490, 533, 735, 769
DinI GGCGCC 1 cut(s) 195
DpnI GATC 2 cut(s) 80, 510
DpnII GATC 2 cut(s) 78, 508
DriI GACNNNNNGTC 1 cut(s) 178
EaeI YGGCCR 2 cut(s) 294, 724
Eam1104I CTCTTC 1 cut(s) 67
Eam1105I GACNNNNNGTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 284
Eco81I CCTNAGG 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 315
EgeI GGCGCC 1 cut(s) 195
EheI GGCGCC 1 cut(s) 195
FaeI CATG 4 cut(s) 44, 334, 499, 748
FaiI YATR 8 cut(s) 42, 332, 400, 473, 497, 635, 746, 752
FalI AAGNNNNNCTT 4 cut(s) 207, 239, 290, 322
FaqI GGGAC 1 cut(s) 779
FatI CATG 4 cut(s) 40, 330, 495, 744
FauI CCCGC 1 cut(s) 149
Fnu4HI GCNGC 7 cut(s) 197, 384, 417, 477, 585, 626, 699
FokI GGATG 1 cut(s) 794
Fsp4HI GCNGC 7 cut(s) 197, 384, 417, 477, 585, 626, 699
FspBI CTAG 1 cut(s) 465
GlaI GCGC 2 cut(s) 160, 195
GluI GCNGC 7 cut(s) 197, 384, 417, 477, 585, 626, 699
GsaI CCCAGC 1 cut(s) 597
GsuI CTGGAG 1 cut(s) 148
HaeII RGCGCY 1 cut(s) 197
HaeIII GGCC 3 cut(s) 296, 335, 726
HapII CCGG 4 cut(s) 200, 281, 392, 665
HhaI GCGC 2 cut(s) 161, 196
Hin1I GRCGYC 1 cut(s) 194
Hin1II CATG 4 cut(s) 44, 334, 499, 748
Hin6I GCGC 2 cut(s) 159, 194
HinP1I GCGC 2 cut(s) 159, 194
HinfI GANTC 7 cut(s) 93, 188, 203, 598, 643, 767, 797
HpaII CCGG 4 cut(s) 200, 281, 392, 665
HphI GGTGA 1 cut(s) 5
Hpy188I TCNGA 6 cut(s) 92, 172, 187, 234, 266, 772
Hpy188III TCNNGA 3 cut(s) 455, 647, 745
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 4 cut(s) 137, 209, 252, 346
HpyCH4V TGCA 6 cut(s) 99, 348, 383, 404, 419, 679
HpyF10VI GCNNNNNNNGC 6 cut(s) 341, 392, 401, 446, 581, 590
HpyF3I CTNAG 5 cut(s) 273, 490, 533, 735, 769
Hsp92I GRCGYC 1 cut(s) 194
Hsp92II CATG 4 cut(s) 44, 334, 499, 748
HspAI GCGC 2 cut(s) 159, 194
KasI GGCGCC 1 cut(s) 193
Kzo9I GATC 2 cut(s) 78, 508
LmnI GCTCC 1 cut(s) 695
Lsp1109I GCAGC 6 cut(s) 395, 403, 463, 596, 637, 710
MaeI CTAG 1 cut(s) 465
MaeIII GTNAC 1 cut(s) 730
MalI GATC 2 cut(s) 80, 510
MboI GATC 2 cut(s) 78, 508
MboII GAAGA 6 cut(s) 54, 57, 60, 63, 66, 215
MfeI CAATTG 1 cut(s) 801
MlsI TGGCCA 2 cut(s) 296, 726
MluCI AATT 6 cut(s) 35, 101, 378, 792, 801, 816
MluNI TGGCCA 2 cut(s) 296, 726
Mly113I GGCGCC 1 cut(s) 194
MlyI GAGTC 3 cut(s) 87, 212, 761
MmeI TCCRAC 4 cut(s) 115, 165, 195, 244
MnlI CCTC 9 cut(s) 26, 35, 98, 128, 133, 148, 172, 645, 772
Mox20I TGGCCA 2 cut(s) 296, 726
MroXI GAANNNNTTC 1 cut(s) 219
MscI TGGCCA 2 cut(s) 296, 726
MseI TTAA 1 cut(s) 146
Msp20I TGGCCA 2 cut(s) 296, 726
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 4 cut(s) 200, 281, 392, 665
MspR9I CCNGG 2 cut(s) 282, 317
MunI CAATTG 1 cut(s) 801
MvaI CCWGG 1 cut(s) 317
MwoI GCNNNNNNNGC 6 cut(s) 341, 392, 401, 446, 581, 590
NarI GGCGCC 1 cut(s) 194
NciI CCSGG 1 cut(s) 282
NdeII GATC 2 cut(s) 78, 508
NlaIII CATG 4 cut(s) 44, 334, 499, 748
NlaIV GGNNCC 3 cut(s) 195, 279, 285
NmeAIII GCCGAG 1 cut(s) 601
NmuCI GTSAC 1 cut(s) 730
PagI TCATGA 1 cut(s) 744
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 4 cut(s) 188, 598, 643, 797
PflFI GACNNNGTC 1 cut(s) 634
PkrI GCNGC 7 cut(s) 198, 385, 418, 478, 586, 627, 700
PleI GAGTC 3 cut(s) 87, 211, 761
PluTI GGCGCC 1 cut(s) 197
PpsI GAGTC 3 cut(s) 87, 211, 761
Psp6I CCWGG 1 cut(s) 315
PspFI CCCAGC 1 cut(s) 593
PspGI CCWGG 1 cut(s) 315
PspN4I GGNNCC 3 cut(s) 195, 279, 285
PspPI GGNCC 1 cut(s) 284
PstNI CAGNNNCTG 1 cut(s) 593
PsyI GACNNNGTC 1 cut(s) 634
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 1 cut(s) 469
RsaNI GTAC 1 cut(s) 468
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 7 cut(s) 197, 384, 417, 477, 585, 626, 699
Sau3AI GATC 2 cut(s) 78, 508
Sau96I GGNCC 1 cut(s) 284
ScaI AGTACT 1 cut(s) 469
SchI GAGTC 3 cut(s) 87, 212, 761
ScrFI CCNGG 2 cut(s) 282, 317
SfoI GGCGCC 1 cut(s) 195
SinI GGWCC 1 cut(s) 284
SmlI CTYRAG 2 cut(s) 570, 714
SmoI CTYRAG 2 cut(s) 570, 714
Sse9I AATT 6 cut(s) 35, 101, 378, 792, 801, 816
SsiI CCGC 2 cut(s) 142, 197
SspDI GGCGCC 1 cut(s) 193
SspMI CTAG 1 cut(s) 465
StyD4I CCNGG 2 cut(s) 280, 315
TaqI TCGA 3 cut(s) 77, 258, 601
TasI AATT 6 cut(s) 35, 101, 378, 792, 801, 816
TatI WGTACW 1 cut(s) 467
TauI GCSGC 1 cut(s) 199
TfiI GAWTC 4 cut(s) 188, 598, 643, 797
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TscAI CASTG 2 cut(s) 254, 522
TseFI GTSAC 1 cut(s) 730
TseI GCWGC 6 cut(s) 383, 416, 476, 584, 625, 698
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 5 cut(s) 17, 215, 328, 733, 761
TspGWI ACGGA 3 cut(s) 100, 276, 280
TspRI CASTG 2 cut(s) 254, 522
Tth111I GACNNNGTC 1 cut(s) 634
VpaK11BI GGWCC 1 cut(s) 284
XapI RAATTY 2 cut(s) 101, 816
XcmI CCANNNNNNNNNTGG 1 cut(s) 304
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 1 cut(s) 465
ZrmI AGTACT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.