Prupe.8G225000_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0006668
Physical Location & Seq
Forward (+)
20254913 .. 20257736
2824 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G225000.1

Sequence Viewer

Length: 858 bp
ATGAAATCTGTTCATTTCTCGCACCTCTCACCTCCAATTCGTGCCTTCAACCCTAATAATCATACTTCTTCATCATCTTTTTCTTCTTCTTCAATCACTCTTCTCCAGCGCTGTAAATTCAGCTCTGTTTCTCTCACCTCCACTTCCAGGCCCTCCCGCTCAATCACGCTTTGCGCTACCAATTCCACCAAAGCGACGTCGTCGTCGTCGTCCCAGTTCGAATCGGCGGCAGCGGAGGGCATAAAGGAGGCTTCTTTTCAGGTTCAAATCGACGCTTCGCAAAGTCCGTGGAATGTGGAAGTCGGAAAACCTAGCGTTCCGTCTCCGTCGGTGGCCAAATTGAGCTTGAGCGACCAGGCTTTCTTCCTCTTGGCCTTCGTCGCCTGCACGACTTCTGTGGCATTTACGAGCCTTGTAATTGCAGCTGTCCCGACACTATTTGCAATGGGGAGAGCTGCAATATCTCTTTCGAAGCTGGCGGATACAGCTCGTGAAGAACTCCCCAGTACTATGGCTGCCATTAGGCTTTCGGGCATGGAAATAAGTGATCTTACGCTGGAACTGAATGATCTAAGCCAAGAGATCGCTGAGGGGGTCAGCAAATCCACTCAAACTGTTCAAGCAGCAGGAGCTGGGATTCGGCAGATTGGTACACTCGCACAGCAGCAGACTATGTCAATGATACAAGAGAGGGCGAGCCTGCCCATCATCTCTTTGCAACCAGCTGTTGTTGGAGCAGCAAAGAAGACTACTCGTGCTGTTGGCCAAGCCACCAGGATCTTCATGAATATAATATCTCCAAAGGACTCGGAAAAAGTCAATGAGGAGGACGTTGAAATTGATAGGGTGGAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

286

Amino Acids

30.19

Weight (kDa)

6.84

Isoelectric Point (pI)

50.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 202
AatII GACGTC 1 cut(s) 200
AccBSI CCGCTC 1 cut(s) 159
AciI CCGC 4 cut(s) 157, 227, 233, 479
AclWI GGATC 1 cut(s) 785
AcoI YGGCCR 2 cut(s) 333, 763
AcsI RAATTY 1 cut(s) 116
AcyI GRCGYC 1 cut(s) 197
AfaI GTAC 2 cut(s) 508, 652
AfeI AGCGCT 1 cut(s) 110
AfiI CCNNNNNNNGG 1 cut(s) 147
AgsI TTSAA 5 cut(s) 49, 93, 266, 620, 836
AjnI CCWGG 3 cut(s) 146, 354, 773
AluBI AGCT 8 cut(s) 123, 345, 425, 455, 475, 488, 632, 725
AluI AGCT 8 cut(s) 123, 345, 425, 455, 475, 488, 632, 725
Alw26I GTCTC 1 cut(s) 327
AlwI GGATC 1 cut(s) 785
AlwNI CAGNNNCTG 1 cut(s) 632
Aor51HI AGCGCT 1 cut(s) 110
AoxI GGCC 4 cut(s) 149, 333, 372, 763
ApeKI GCWGC 7 cut(s) 230, 422, 455, 515, 623, 664, 737
ApoI RAATTY 1 cut(s) 116
AspLEI GCGC 2 cut(s) 111, 176
AspS9I GGNCC 1 cut(s) 150
AsuHPI GGTGA 2 cut(s) 21, 127
AsuII TTCGAA 2 cut(s) 219, 470
BalI TGGCCA 2 cut(s) 335, 765
BauI CACGAG 2 cut(s) 489, 753
BbsI GAAGAC 1 cut(s) 752
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 7 cut(s) 242, 434, 442, 502, 635, 676, 749
BccI CCATC 1 cut(s) 713
BciT130I CCWGG 3 cut(s) 148, 356, 775
BciVI GTATCC 1 cut(s) 475
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 1 cut(s) 312
BfoI RGCGCY 1 cut(s) 112
BfuI GTATCC 1 cut(s) 475
BisI GCNGC 8 cut(s) 228, 231, 423, 456, 516, 624, 665, 738
BlsI GCNGC 8 cut(s) 229, 232, 424, 457, 517, 625, 666, 739
BmcAI AGTACT 1 cut(s) 508
Bme1390I CCNGG 3 cut(s) 148, 356, 775
BmgT120I GGNCC 1 cut(s) 150
BmrFI CCNGG 3 cut(s) 148, 356, 775
BmrI ACTGGG 2 cut(s) 208, 498
BmuI ACTGGG 2 cut(s) 208, 498
BpiI GAAGAC 1 cut(s) 752
BpmI CTGGAG 1 cut(s) 89
Bpu10I CCTNAGC 1 cut(s) 588
Bpu14I TTCGAA 2 cut(s) 219, 470
BpuEI CTTGAG 1 cut(s) 367
BsaHI GRCGYC 1 cut(s) 197
BsaJI CCNNGG 1 cut(s) 287
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse1I ACTGG 2 cut(s) 214, 504
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 3 cut(s) 148, 356, 775
BseDI CCNNGG 1 cut(s) 287
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 1 cut(s) 579
BseNI ACTGG 2 cut(s) 214, 504
BseRI GAGGAG 1 cut(s) 839
BseXI GCAGC 7 cut(s) 242, 434, 442, 502, 635, 676, 749
BseYI CCCAGC 1 cut(s) 632
BsgI GTGCAG 1 cut(s) 370
BshFI GGCC 4 cut(s) 151, 335, 374, 765
BslFI GGGAC 2 cut(s) 196, 413
BslI CCNNNNNNNGG 1 cut(s) 147
BsmAI GTCTC 1 cut(s) 327
BsmBI CGTCTC 1 cut(s) 327
BsmFI GGGAC 2 cut(s) 196, 413
BsnI GGCC 4 cut(s) 151, 335, 374, 765
Bsp119I TTCGAA 2 cut(s) 219, 470
Bsp143I GATC 4 cut(s) 547, 568, 582, 777
BspACI CCGC 4 cut(s) 157, 227, 233, 479
BspANI GGCC 4 cut(s) 151, 335, 374, 765
BspCNI CTCAG 1 cut(s) 580
BspHI TCATGA 1 cut(s) 783
BspPI GGATC 1 cut(s) 785
BspT104I TTCGAA 2 cut(s) 219, 470
BsrBI CCGCTC 1 cut(s) 159
BsrDI GCAATG 1 cut(s) 450
BsrI ACTGG 2 cut(s) 214, 504
BssECI CCNNGG 1 cut(s) 287
BssMI GATC 4 cut(s) 547, 568, 582, 777
BssNI GRCGYC 1 cut(s) 197
BssSI CACGAG 2 cut(s) 489, 753
Bst2BI CACGAG 2 cut(s) 489, 753
Bst2UI CCWGG 3 cut(s) 148, 356, 775
Bst4CI ACNGT 1 cut(s) 616
Bst6I CTCTTC 1 cut(s) 105
BstACI GRCGYC 1 cut(s) 197
BstBI TTCGAA 2 cut(s) 219, 470
BstC8I GCNNGC 4 cut(s) 385, 477, 697, 701
BstDEI CTNAG 2 cut(s) 572, 588
BstDSI CCRYGG 1 cut(s) 287
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 2 cut(s) 111, 176
BstKTI GATC 4 cut(s) 550, 571, 585, 780
BstMAI GTCTC 1 cut(s) 327
BstMBI GATC 4 cut(s) 547, 568, 582, 777
BstMWI GCNNNNNNNGC 3 cut(s) 380, 485, 629
BstNI CCWGG 3 cut(s) 148, 356, 775
BstSCI CCNGG 3 cut(s) 146, 354, 773
BstV1I GCAGC 7 cut(s) 242, 434, 442, 502, 635, 676, 749
BstV2I GAAGAC 1 cut(s) 752
BstX2I RGATCY 1 cut(s) 777
BstXI CCANNNNNNTGG 1 cut(s) 511
BstYI RGATCY 1 cut(s) 777
BsuI GTATCC 1 cut(s) 475
BsuRI GGCC 4 cut(s) 151, 335, 374, 765
BtgI CCRYGG 1 cut(s) 287
Cac8I GCNNGC 4 cut(s) 385, 477, 697, 701
CaiI CAGNNNCTG 1 cut(s) 632
CciI TCATGA 1 cut(s) 783
CfoI GCGC 2 cut(s) 111, 176
Cfr13I GGNCC 1 cut(s) 150
CseI GACGC 1 cut(s) 281
Csp6I GTAC 2 cut(s) 507, 651
CspCI CAANNNNNGTGG 2 cut(s) 269, 304
CviAII CATG 2 cut(s) 535, 784
CviQI GTAC 2 cut(s) 507, 651
DdeI CTNAG 2 cut(s) 572, 588
DpnI GATC 4 cut(s) 549, 570, 584, 779
DpnII GATC 4 cut(s) 547, 568, 582, 777
DrdI GACNNNNNNGTC 1 cut(s) 202
DseDI GACNNNNNNGTC 1 cut(s) 202
EaeI YGGCCR 2 cut(s) 333, 763
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
EciI GGCGGA 1 cut(s) 494
Eco47III AGCGCT 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 150
EcoRII CCWGG 3 cut(s) 146, 354, 773
Esp3I CGTCTC 1 cut(s) 327
FaeI CATG 2 cut(s) 538, 787
FaiI YATR 7 cut(s) 63, 242, 512, 536, 674, 785, 791
FaqI GGGAC 2 cut(s) 196, 413
FatI CATG 2 cut(s) 534, 783
FauI CCCGC 1 cut(s) 164
Fnu4HI GCNGC 8 cut(s) 228, 231, 423, 456, 516, 624, 665, 738
Fsp4HI GCNGC 8 cut(s) 228, 231, 423, 456, 516, 624, 665, 738
FspBI CTAG 1 cut(s) 312
GlaI GCGC 2 cut(s) 110, 175
GluI GCNGC 8 cut(s) 228, 231, 423, 456, 516, 624, 665, 738
GsaI CCCAGC 1 cut(s) 636
GsuI CTGGAG 1 cut(s) 89
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 4 cut(s) 151, 335, 374, 765
HgaI GACGC 1 cut(s) 281
HhaI GCGC 2 cut(s) 111, 176
Hin1I GRCGYC 1 cut(s) 197
Hin1II CATG 2 cut(s) 538, 787
Hin6I GCGC 2 cut(s) 109, 174
HinP1I GCGC 2 cut(s) 109, 174
HinfI GANTC 3 cut(s) 221, 637, 806
HphI GGTGA 2 cut(s) 21, 127
Hpy166II GTNNAC 1 cut(s) 653
Hpy188I TCNGA 2 cut(s) 305, 811
Hpy188III TCNNGA 3 cut(s) 430, 491, 784
Hpy8I GTNNAC 1 cut(s) 653
Hpy99I CGWCG 8 cut(s) 199, 202, 205, 208, 211, 275, 331, 383
HpyAV CCTTC 2 cut(s) 55, 385
HpyCH4III ACNGT 1 cut(s) 616
HpyCH4IV ACGT 2 cut(s) 197, 831
HpyCH4V TGCA 5 cut(s) 387, 422, 443, 458, 718
HpyF10VI GCNNNNNNNGC 3 cut(s) 380, 485, 629
HpyF3I CTNAG 2 cut(s) 572, 588
HpySE526I ACGT 2 cut(s) 197, 831
Hsp92I GRCGYC 1 cut(s) 197
Hsp92II CATG 2 cut(s) 538, 787
HspAI GCGC 2 cut(s) 109, 174
Kzo9I GATC 4 cut(s) 547, 568, 582, 777
LmnI GCTCC 2 cut(s) 629, 734
Lsp1109I GCAGC 7 cut(s) 242, 434, 442, 502, 635, 676, 749
MaeI CTAG 1 cut(s) 312
MaeII ACGT 2 cut(s) 197, 831
MalI GATC 4 cut(s) 549, 570, 584, 779
MbiI CCGCTC 1 cut(s) 159
MboI GATC 4 cut(s) 547, 568, 582, 777
MboII GAAGA 9 cut(s) 60, 75, 78, 81, 92, 355, 506, 757, 772
MflI RGATCY 1 cut(s) 777
MlsI TGGCCA 2 cut(s) 335, 765
MluCI AATT 6 cut(s) 36, 116, 181, 338, 417, 837
MluNI TGGCCA 2 cut(s) 335, 765
MlyI GAGTC 1 cut(s) 800
MmeI TCCRAC 2 cut(s) 283, 712
Mox20I TGGCCA 2 cut(s) 335, 765
MscI TGGCCA 2 cut(s) 335, 765
MseI TTAA 1 cut(s) 856
Msp20I TGGCCA 2 cut(s) 335, 765
MspA1I CMGCKG 3 cut(s) 233, 425, 725
MspR9I CCNGG 3 cut(s) 148, 356, 775
MvaI CCWGG 3 cut(s) 148, 356, 775
MwoI GCNNNNNNNGC 3 cut(s) 380, 485, 629
NdeII GATC 4 cut(s) 547, 568, 582, 777
NlaIII CATG 2 cut(s) 538, 787
NspV TTCGAA 2 cut(s) 219, 470
PagI TCATGA 1 cut(s) 783
PcsI WCGNNNNNNNCGW 3 cut(s) 203, 206, 284
PfeI GAWTC 2 cut(s) 221, 637
PflFI GACNNNGTC 2 cut(s) 199, 673
PkrI GCNGC 8 cut(s) 229, 232, 424, 457, 517, 625, 666, 739
PleI GAGTC 1 cut(s) 800
PpsI GAGTC 1 cut(s) 800
Psp6I CCWGG 3 cut(s) 146, 354, 773
PspFI CCCAGC 1 cut(s) 632
PspGI CCWGG 3 cut(s) 146, 354, 773
PspPI GGNCC 1 cut(s) 150
PstNI CAGNNNCTG 1 cut(s) 632
PsuI RGATCY 1 cut(s) 777
PsyI GACNNNGTC 2 cut(s) 199, 673
PvuII CAGCTG 2 cut(s) 425, 725
RsaI GTAC 2 cut(s) 508, 652
RsaNI GTAC 2 cut(s) 507, 651
SaqAI TTAA 1 cut(s) 856
SatI GCNGC 8 cut(s) 228, 231, 423, 456, 516, 624, 665, 738
Sau3AI GATC 4 cut(s) 547, 568, 582, 777
Sau96I GGNCC 1 cut(s) 150
ScaI AGTACT 1 cut(s) 508
SchI GAGTC 1 cut(s) 800
ScrFI CCNGG 3 cut(s) 148, 356, 775
SfuI TTCGAA 2 cut(s) 219, 470
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
Sse9I AATT 6 cut(s) 36, 116, 181, 338, 417, 837
SsiI CCGC 4 cut(s) 157, 227, 233, 479
SspMI CTAG 1 cut(s) 312
StyD4I CCNGG 3 cut(s) 146, 354, 773
TaaI ACNGT 1 cut(s) 616
TaiI ACGT 2 cut(s) 200, 834
TaqI TCGA 3 cut(s) 219, 270, 470
TasI AATT 6 cut(s) 36, 116, 181, 338, 417, 837
TatI WGTACW 1 cut(s) 506
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 2 cut(s) 221, 637
Tru1I TTAA 1 cut(s) 856
Tru9I TTAA 1 cut(s) 856
TseI GCWGC 7 cut(s) 230, 422, 455, 515, 623, 664, 737
TspDTI ATGAA 4 cut(s) 17, 60, 772, 800
TspGWI ACGGA 3 cut(s) 276, 309, 315
Tth111I GACNNNGTC 2 cut(s) 199, 673
XapI RAATTY 1 cut(s) 116
XspI CTAG 1 cut(s) 312
ZraI GACGTC 1 cut(s) 198
ZrmI AGTACT 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.