FvH4_2g22220

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003313
Physical Location & Seq
Reverse (-)
18262662 .. 18267312
4651 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g22220.t1

Sequence Viewer

Length: 2796 bp
ATGGCAGCAAAGGGGAAGTCATCAGTAGATGTGCAGAGGAATTTTCAGGGGTTTATAGCCATTCTAACATCTGCAGCATGTGAATGGTTTTTGATATTTCTTCTGTTAGTCGATGCACTGTTATCCTATCTGCTGACTAAGTTTGCGGTCTATTGTGATCTGCAAACTCCTTGCATCGTGTGCTCAAGGTTTGAACATATTACTGGCAAGGAAAGCTCTCAACTTTACGAGGAACTACTTTGCAGCAACCACATATCAAAGCTCGCATCTTGGATAGCTAGTCAAAATTATGGTAAGGATGATGACAATGAAGATTACTTCTTTCCTTTCACCACAAGGGACTACTCCAACCGAGAAATGCATATGTTTTCATATGGTAATTCGGTATCAGATATTGAAAGTTCTGGTCTTCGGAGGCGTGTGGTTGGCAGAAGTTGTGTTCATAGTCCTGTTCATAGCCCTGTTCATAGTTCTGTGGATGTAGGTCAATGGTCTCGTTGCTCTAGCCCTTGGGGACGTAGAGGAAATCCTAAAGGACTAGAACTCAAATCATCTGGGAAAAAAAATCCCAAGCCTAATATTCCTTTGCCACGTTTGCCAAACCGTAGCCGTTCAAATCGAGACCAGGATAGTTCAAAGAAGACCAGGGATAAGTCTCCGGCGTCAGCTACTAGTCGTCATTCTGCAAAAGTAGGAGTTGATCCCTTGTCACATGTAGGATACAGTGAATTGAATCTCTTTTCTGATACTGACTCAGAGTCTCTTTTTACTGATGGGGATGATGGAAGCAATGTATCCAGTGCAAGTCATGAGCCTAAGATGGAATGTTCCAGACGTGCTTCACGGTATCGGCCTAAAGCACCACCAAATGGTTTGAAGTCAAGAAGGCATAGAAATCGGTCCTCCAAACCCAAGCGGTCACTTTCAGAACCTTGGCCTGTTTTTAGGAAATCCTCTGATGTAAAATATTTGGGTTCTGATAATGAATTTGAGATTGGCTTCCGTGAGCTTAACTATCAGCAATCCAATCAGAAGTCCAACCCTTCTGCATTACCTGAGCTTATTTCACTGGATGACGTCTTTGCGTCACCCAGTGTACAAGAAAAGGATATTAATGGGACAGGCGACTTTGGATTTGCATCTTTCAATAAGAATGTCGAACTTTTGAAGTCTATTTGTGCAAATGACGGAGCATCTGATAAAAATGATCCAGCTGTTATAAAATCAAACCAAAGTAATGTAAATGCCATGTCTGAGTCCGAGGGAAGTGCTAGGGAAAAGGATTCATCTAGTATTACTACAGAAGCTCCTCTTATAAAACCAAGCCCTAGTAATGTAGATCCTGTGTCCAAGTCACAGGGATGTGTTCAGGAACGAGATTCATCAGGTATTACTACAGACGCACTTATAAGTTCAAACCAAAATGCTATGTCTGAGTCAGAAGGAATTGGCAAAGAAAGAGATTCATTGGGTATTACTACAGAAGCACTTAGAAGCCCATACCACAGTAACTTAAATGCTATGTCTGAGAAAATGAAACTGTTAGCTCAACAGGACTCTTCTGCTCGGGAGATTAAGTTCTCACCAAAGAATACAAGTTCGACAGTAGATGGTTATCTGGGTGAAAAACAGACTACTGATGCTTCAAGCTCTAATGCAAACCCGATGCTTCACTCGTCAGCATCAGTAGAATCGGGTCTTGAATCGCTGGATGGAGGCTATGTCAGTGAAATTGAAGGTGAAAGTATAGTTGATCGGTTAAAACGACAGGTTGAGTATGACAGGAAATGCATAAAGGAGCTGTACAGGGAATTGGAAGAAGAAAGAAGTGCAGCTGCAATTGCTGCAAATCAGGCAATGGCCATGATCACTAAGCTCCAAGAAGAGAAAGCTGCATTCCATATGGAGGCCCTTCACTACTTAAGAATGATGGAAGAGCAGGCTGAGTTTGATGTGGAGGCACTGGAGAAAGCCAATGATCTCCTGGCTGAAAAGGAGAAAGAGATACAGGACTTGGAAGCAGAGCTAGAGTTTTCTAAGTTAGAATTCCAAGATGAATCAATGATGGAGAATCTAAATGCCAGAACTTCGGATTTAGTGGGTGGGATTGAACCGGTGGAGAGTACTGTTGTGCCTGGTGTGAATAACGATCTCACTGTTACTTGCAACTCAATGCCTACTGACCTACCGATAGTCAGTAACCAACCTCCTTTTTCTGAAACTACTCTTCTAGAGTTTGAAGATGAAAAACTGTACATTACAAATTGTTTGAAGAGCTTGGAGAGGAAGCTTTCAGAAATTTACAGTAGGGGGGCTTCCTCCAAAATGCTTAATGGTGAAAATCAAGCAGAGTCTCCTAGAAAAGAGAAGAGTCTATTAAATGATGGAAAGAATCAAGAAGAGACTCCTAGGAAAGAGAGGCATGAATTATATGGTCAGCTTGAAGAGAATGTTCTGCATGGGAAAAAAGATTTACATATGTCTAACGGAAGACCTGCTGCTCGAGAAGGGCAAACTGATTGTGATGGTGGTGGTGATTTAGAATGTAAAGAAGCCATTAATGATTCTGATTTCCATGGGGAAAATAATATCATGGATCCTAGAGAAATGGATATGGTTATTCTGGAAAATGAAATCTCAGACCTTAATGATAGGTTGGAGGCACTTGAGACTGATCATGATTTTCTTGAGCACATGCTCTACTCTCTTCAAAATGGAGCGGAAGGACTACGATTTGTTCGAGAGATAGCTCATCAGCTGCAAGAGTTGCGGAAGATAGGGAGGGGTTTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

932

Amino Acids

103.72

Weight (kDa)

5.14

Isoelectric Point (pI)

52.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 586 - 676 1.6e-29 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1220, 1316, 1409
AatII GACGTC 1 cut(s) 1080
Acc36I ACCTGC 1 cut(s) 2503
AccB7I CCANNNNNTGG 1 cut(s) 869
AccBSI CCGCTC 1 cut(s) 2720
AciI CCGC 4 cut(s) 146, 916, 2720, 2770
AclWI GGATC 5 cut(s) 695, 1202, 1334, 2591, 2604
AcoI YGGCCR 1 cut(s) 1860
AcsI RAATTY 4 cut(s) 40, 986, 2045, 2298
AcyI GRCGYC 2 cut(s) 662, 1077
AdeI CACNNNGTG 1 cut(s) 1094
AfaI GTAC 4 cut(s) 1098, 1805, 2125, 2255
AfiI CCNNNNNNNGG 2 cut(s) 869, 1906
AflII CTTAAG 1 cut(s) 1921
AflIII ACRYGT 1 cut(s) 712
AgeI ACCGGT 1 cut(s) 2113
AhdI GACNNNNNGTC 1 cut(s) 757
AhlI ACTAGT 1 cut(s) 671
AjiI CACGTC 1 cut(s) 836
AjnI CCWGG 4 cut(s) 624, 644, 1983, 2134
AjuI GAANNNNNNNTTGG 2 cut(s) 978, 1010
AloI GAACNNNNNNTCC 2 cut(s) 1562, 1594
Alw21I GWGCWC 2 cut(s) 185, 2694
Alw26I GTCTC 7 cut(s) 498, 615, 660, 765, 2358, 2396, 2663
AlwI GGATC 5 cut(s) 695, 1202, 1334, 2591, 2604
Ama87I CYCGRG 2 cut(s) 1566, 2502
AoxI GGCC 4 cut(s) 851, 935, 1860, 1908
ApeKI GCWGC 9 cut(s) 5, 74, 243, 1832, 1835, 1844, 1892, 2498, 2758
ApoI RAATTY 4 cut(s) 40, 986, 2045, 2298
ArsI GACNNNNNNTTYG 3 cut(s) 34, 1118, 1150
AseI ATTAAT 2 cut(s) 1113, 2559
AsiGI ACCGGT 1 cut(s) 2113
AspA2I CCTAGG 1 cut(s) 2408
AspS9I GGNCC 2 cut(s) 900, 1909
AsuHPI GGTGA 7 cut(s) 322, 1080, 1575, 1634, 1751, 2348, 2546
AvaI CYCGRG 2 cut(s) 1566, 2502
AvaII GGWCC 1 cut(s) 900
AvrII CCTAGG 1 cut(s) 2408
BalI TGGCCA 1 cut(s) 1862
BamHI GGATCC 1 cut(s) 2596
BarI GAAGNNNNNNTAC 2 cut(s) 2299, 2331
BbsI GAAGAC 3 cut(s) 401, 647, 2497
Bbv12I GWGCWC 2 cut(s) 185, 2694
BbvI GCAGC 9 cut(s) 17, 86, 255, 1822, 1831, 1844, 1879, 2485, 2745
BccI CCATC 9 cut(s) 767, 776, 814, 1604, 1706, 1924, 2059, 2378, 2519
BceAI ACGGC 1 cut(s) 594
BcgI CGANNNNNNTGC 4 cut(s) 878, 912, 1250, 1284
BciT130I CCWGG 4 cut(s) 626, 646, 1985, 2136
BciVI GTATCC 2 cut(s) 713, 805
BclI TGATCA 2 cut(s) 1866, 2674
BcoDI GTCTC 7 cut(s) 498, 615, 660, 765, 2358, 2396, 2663
BcuI ACTAGT 1 cut(s) 671
BfmI CTRYAG 4 cut(s) 72, 1299, 1395, 1479
BfrI CTTAAG 1 cut(s) 1921
BfuAI ACCTGC 1 cut(s) 2503
BfuI GTATCC 2 cut(s) 713, 805
BisI GCNGC 9 cut(s) 6, 75, 244, 1833, 1836, 1845, 1893, 2499, 2759
BlnI CCTAGG 1 cut(s) 2408
BlsI GCNGC 9 cut(s) 7, 76, 245, 1834, 1837, 1846, 1894, 2500, 2760
BmcAI AGTACT 1 cut(s) 2125
Bme1390I CCNGG 4 cut(s) 626, 646, 1985, 2136
Bme18I GGWCC 1 cut(s) 900
BmeRI GACNNNNNGTC 1 cut(s) 757
BmeT110I CYCGRG 2 cut(s) 1566, 2502
BmgBI CACGTC 1 cut(s) 836
BmgT120I GGNCC 2 cut(s) 900, 1909
BmiI GGNNCC 1 cut(s) 2598
BmrFI CCNGG 4 cut(s) 626, 646, 1985, 2136
BmrI ACTGGG 1 cut(s) 1086
BmsI GCATC 8 cut(s) 103, 183, 275, 1148, 1202, 1630, 1656, 1691
BmuI ACTGGG 1 cut(s) 1086
BpiI GAAGAC 3 cut(s) 401, 647, 2497
BpmI CTGGAG 1 cut(s) 1985
Bpu10I CCTNAGC 1 cut(s) 1056
BpuEI CTTGAG 3 cut(s) 169, 2687, 2708
BsaBI GATNNNNATC 2 cut(s) 1138, 1614
BsaHI GRCGYC 2 cut(s) 662, 1077
BsaI GGTCTC 2 cut(s) 498, 615
BsaJI CCNNGG 6 cut(s) 509, 645, 932, 1260, 2408, 2575
BsaWI WCCGGW 1 cut(s) 2113
BsaXI ACNNNNNCTCC 6 cut(s) 1291, 1321, 1562, 1592, 1707, 1737
Bsc4I CCNNNNNNNGG 2 cut(s) 869, 1906
Bse118I RCCGGY 1 cut(s) 2113
Bse1I ACTGG 5 cut(s) 208, 798, 1074, 1092, 1968
Bse3DI GCAATG 2 cut(s) 796, 1863
Bse8I GATNNNNATC 2 cut(s) 1138, 1614
BseBI CCWGG 4 cut(s) 626, 646, 1985, 2136
BseDI CCNNGG 6 cut(s) 509, 645, 932, 1260, 2408, 2575
BseGI GGATG 6 cut(s) 304, 484, 784, 1078, 1367, 1717
BseJI GATNNNNATC 2 cut(s) 1138, 1614
BseLI CCNNNNNNNGG 2 cut(s) 869, 1906
BseMI GCAATG 2 cut(s) 796, 1863
BseMII CTCAG 7 cut(s) 768, 1047, 1245, 1425, 1518, 1935, 2652
BseNI ACTGG 5 cut(s) 208, 798, 1074, 1092, 1968
BseRI GAGGAG 1 cut(s) 1299
BseXI GCAGC 9 cut(s) 17, 86, 255, 1822, 1831, 1844, 1879, 2485, 2745
BsgI GTGCAG 2 cut(s) 53, 1851
BshFI GGCC 4 cut(s) 853, 937, 1862, 1910
BshTI ACCGGT 1 cut(s) 2113
BsiHKAI GWGCWC 2 cut(s) 185, 2694
BsiHKCI CYCGRG 2 cut(s) 1566, 2502
BsiSI CCGG 2 cut(s) 659, 2114
BslFI GGGAC 3 cut(s) 353, 528, 1132
BslI CCNNNNNNNGG 2 cut(s) 869, 1906
BsmAI GTCTC 7 cut(s) 498, 615, 660, 765, 2358, 2396, 2663
BsmFI GGGAC 3 cut(s) 353, 528, 1132
BsmI GAATGC 1 cut(s) 1895
BsnI GGCC 4 cut(s) 853, 937, 1862, 1910
Bso31I GGTCTC 2 cut(s) 498, 615
BsoBI CYCGRG 2 cut(s) 1566, 2502
Bsp1286I GDGCHC 2 cut(s) 185, 2694
Bsp1407I TGTACA 3 cut(s) 1096, 1803, 2253
Bsp19I CCATGG 1 cut(s) 2575
BspACI CCGC 4 cut(s) 146, 916, 2720, 2770
BspANI GGCC 4 cut(s) 853, 937, 1862, 1910
BspCNI CTCAG 7 cut(s) 767, 1048, 1246, 1426, 1519, 1936, 2651
BspHI TCATGA 2 cut(s) 808, 2677
BspLI GGNNCC 1 cut(s) 2598
BspMAI CTGCAG 1 cut(s) 76
BspMI ACCTGC 1 cut(s) 2503
BspPI GGATC 5 cut(s) 695, 1202, 1334, 2591, 2604
BspQI GCTCTTC 2 cut(s) 1929, 2267
BspTI CTTAAG 1 cut(s) 1921
BspTNI GGTCTC 2 cut(s) 498, 615
BsrBI CCGCTC 1 cut(s) 2720
BsrDI GCAATG 2 cut(s) 796, 1863
BsrFI RCCGGY 1 cut(s) 2113
BsrGI TGTACA 3 cut(s) 1096, 1803, 2253
BsrI ACTGG 5 cut(s) 208, 798, 1074, 1092, 1968
BssAI RCCGGY 1 cut(s) 2113
BssECI CCNNGG 6 cut(s) 509, 645, 932, 1260, 2408, 2575
BssNI GRCGYC 2 cut(s) 662, 1077
BssT1I CCWWGG 4 cut(s) 509, 932, 2408, 2575
Bst2UI CCWGG 4 cut(s) 626, 646, 1985, 2136
Bst6I CTCTTC 9 cut(s) 1564, 1878, 1929, 2232, 2267, 2363, 2394, 2439, 2712
BstACI GRCGYC 2 cut(s) 662, 1077
BstAFI CTTAAG 1 cut(s) 1921
BstAPI GCANNNNNTGC 3 cut(s) 180, 1844, 2767
BstAUI TGTACA 3 cut(s) 1096, 1803, 2253
BstC8I GCNNGC 2 cut(s) 264, 1941
BstDSI CCRYGG 1 cut(s) 2575
BstF5I GGATG 6 cut(s) 304, 484, 784, 1078, 1367, 1717
BstMAI GTCTC 7 cut(s) 498, 615, 660, 765, 2358, 2396, 2663
BstMWI GCNNNNNNNGC 7 cut(s) 180, 213, 595, 1841, 1844, 1853, 2767
BstNI CCWGG 4 cut(s) 626, 646, 1985, 2136
BstNSI RCATGY 3 cut(s) 81, 716, 2698
BstSCI CCNGG 4 cut(s) 624, 644, 1983, 2134
BstSFI CTRYAG 4 cut(s) 72, 1299, 1395, 1479
BstV1I GCAGC 9 cut(s) 17, 86, 255, 1822, 1831, 1844, 1879, 2485, 2745
BstV2I GAAGAC 3 cut(s) 401, 647, 2497
BstX2I RGATCY 2 cut(s) 1339, 2596
BstYI RGATCY 2 cut(s) 1339, 2596
BsuI GTATCC 2 cut(s) 713, 805
BsuRI GGCC 4 cut(s) 853, 937, 1862, 1910
BtgI CCRYGG 1 cut(s) 2575
BtrI CACGTC 1 cut(s) 836
BtsCI GGATG 6 cut(s) 304, 484, 784, 1078, 1367, 1717
BtsIMutI CAGTG 8 cut(s) 116, 730, 805, 1067, 1099, 1732, 1961, 2154
BveI ACCTGC 1 cut(s) 2503
Cac8I GCNNGC 2 cut(s) 264, 1941
CciI TCATGA 2 cut(s) 808, 2677
Cfr10I RCCGGY 1 cut(s) 2113
Cfr13I GGNCC 2 cut(s) 900, 1909
CseI GACGC 3 cut(s) 651, 1074, 1409
Csp6I GTAC 4 cut(s) 1097, 1804, 2124, 2254
CspAI ACCGGT 1 cut(s) 2113
CviQI GTAC 4 cut(s) 1097, 1804, 2124, 2254
DraIII CACNNNGTG 1 cut(s) 1094
DriI GACNNNNNGTC 1 cut(s) 757
EaeI YGGCCR 1 cut(s) 1860
Eam1104I CTCTTC 9 cut(s) 1564, 1878, 1929, 2232, 2267, 2363, 2394, 2439, 2712
Eam1105I GACNNNNNGTC 1 cut(s) 757
EarI CTCTTC 9 cut(s) 1564, 1878, 1929, 2232, 2267, 2363, 2394, 2439, 2712
Eco130I CCWWGG 4 cut(s) 509, 932, 2408, 2575
Eco31I GGTCTC 2 cut(s) 498, 615
Eco47I GGWCC 1 cut(s) 900
Eco88I CYCGRG 2 cut(s) 1566, 2502
EcoO109I RGGNCCY 1 cut(s) 1909
EcoRI GAATTC 1 cut(s) 2045
EcoRII CCWGG 4 cut(s) 624, 644, 1983, 2134
EcoT14I CCWWGG 4 cut(s) 509, 932, 2408, 2575
EcoT22I ATGCAT 2 cut(s) 363, 1793
ErhI CCWWGG 4 cut(s) 509, 932, 2408, 2575
FalI AAGNNNNNCTT 2 cut(s) 1296, 1328
FaqI GGGAC 3 cut(s) 353, 528, 1132
FauNDI CATATG 4 cut(s) 363, 373, 1902, 2478
FbaI TGATCA 2 cut(s) 1866, 2674
Fnu4HI GCNGC 9 cut(s) 6, 75, 244, 1833, 1836, 1845, 1893, 2499, 2759
FokI GGATG 6 cut(s) 311, 491, 791, 1085, 1374, 1724
Fsp4HI GCNGC 9 cut(s) 6, 75, 244, 1833, 1836, 1845, 1893, 2499, 2759
GluI GCNGC 9 cut(s) 6, 75, 244, 1833, 1836, 1845, 1893, 2499, 2759
GsuI CTGGAG 1 cut(s) 1985
HaeIII GGCC 4 cut(s) 853, 937, 1862, 1910
HapII CCGG 2 cut(s) 659, 2114
HgaI GACGC 3 cut(s) 651, 1074, 1409
Hin1I GRCGYC 2 cut(s) 662, 1077
HindIII AAGCTT 1 cut(s) 2288
HpaII CCGG 2 cut(s) 659, 2114
HphI GGTGA 7 cut(s) 322, 1080, 1575, 1634, 1751, 2348, 2546
Hpy166II GTNNAC 1 cut(s) 1097
Hpy8I GTNNAC 1 cut(s) 1097
HpyAV CCTTC 7 cut(s) 879, 1053, 1436, 1730, 1922, 2501, 2717
HpyCH4IV ACGT 4 cut(s) 517, 592, 835, 1077
HpyF10VI GCNNNNNNNGC 7 cut(s) 180, 213, 595, 1841, 1844, 1853, 2767
HpySE526I ACGT 4 cut(s) 517, 592, 835, 1077
Hsp92I GRCGYC 2 cut(s) 662, 1077
Ksp22I TGATCA 2 cut(s) 1866, 2674
LguI GCTCTTC 2 cut(s) 1929, 2267
LmnI GCTCC 5 cut(s) 1190, 1312, 1798, 1881, 2717
Lsp1109I GCAGC 9 cut(s) 17, 86, 255, 1822, 1831, 1844, 1879, 2485, 2745
LweI GCATC 8 cut(s) 103, 183, 275, 1148, 1202, 1630, 1656, 1691
MaeII ACGT 4 cut(s) 517, 592, 835, 1077
MaeIII GTNAC 7 cut(s) 708, 918, 1086, 1353, 1508, 2158, 2198
MbiI CCGCTC 1 cut(s) 2720
MfeI CAATTG 1 cut(s) 1839
MflI RGATCY 2 cut(s) 1339, 2596
MhlI GDGCHC 2 cut(s) 185, 2694
MlsI TGGCCA 1 cut(s) 1862
MluNI TGGCCA 1 cut(s) 1862
MlyI GAGTC 8 cut(s) 746, 767, 1265, 1445, 1550, 2360, 2380, 2398
MmeI TCCRAC 3 cut(s) 372, 1062, 2637
Mox20I TGGCCA 1 cut(s) 1862
Mph1103I ATGCAT 2 cut(s) 363, 1793
MscI TGGCCA 1 cut(s) 1862
MslI CAYNNNNRTG 2 cut(s) 82, 1360
Msp20I TGGCCA 1 cut(s) 1862
MspA1I CMGCKG 3 cut(s) 1214, 1835, 2758
MspCI CTTAAG 1 cut(s) 1921
MspI CCGG 2 cut(s) 659, 2114
MspR9I CCNGG 4 cut(s) 626, 646, 1985, 2136
MunI CAATTG 1 cut(s) 1839
Mva1269I GAATGC 1 cut(s) 1895
MvaI CCWGG 4 cut(s) 626, 646, 1985, 2136
MwoI GCNNNNNNNGC 7 cut(s) 180, 213, 595, 1841, 1844, 1853, 2767
NcoI CCATGG 1 cut(s) 2575
NdeI CATATG 4 cut(s) 363, 373, 1902, 2478
NlaIV GGNNCC 1 cut(s) 2598
NmuCI GTSAC 4 cut(s) 708, 918, 1086, 1353
NsiI ATGCAT 2 cut(s) 363, 1793
NspI RCATGY 3 cut(s) 81, 716, 2698
PaeR7I CTCGAG 1 cut(s) 2502
PagI TCATGA 2 cut(s) 808, 2677
PciI ACATGT 1 cut(s) 712
PciSI GCTCTTC 2 cut(s) 1929, 2267
PcsI WCGNNNNNNNCGW 2 cut(s) 1762, 2737
PctI GAATGC 1 cut(s) 1895
PflMI CCANNNNNTGG 1 cut(s) 869
PinAI ACCGGT 1 cut(s) 2113
PkrI GCNGC 9 cut(s) 7, 76, 245, 1834, 1837, 1846, 1894, 2500, 2760
PleI GAGTC 8 cut(s) 746, 766, 1264, 1444, 1550, 2359, 2379, 2398
PpsI GAGTC 8 cut(s) 746, 766, 1264, 1444, 1550, 2359, 2379, 2398
PscI ACATGT 1 cut(s) 712
PshBI ATTAAT 2 cut(s) 1113, 2559
PsiI TTATAA 3 cut(s) 1220, 1316, 1409
Psp6I CCWGG 4 cut(s) 624, 644, 1983, 2134
PspGI CCWGG 4 cut(s) 624, 644, 1983, 2134
PspN4I GGNNCC 1 cut(s) 2598
PspPI GGNCC 2 cut(s) 900, 1909
PstI CTGCAG 1 cut(s) 76
PsuI RGATCY 2 cut(s) 1339, 2596
PvuII CAGCTG 3 cut(s) 1214, 1835, 2758
RsaI GTAC 4 cut(s) 1098, 1805, 2125, 2255
RsaNI GTAC 4 cut(s) 1097, 1804, 2124, 2254
RseI CAYNNNNRTG 2 cut(s) 82, 1360
SapI GCTCTTC 2 cut(s) 1929, 2267
SatI GCNGC 9 cut(s) 6, 75, 244, 1833, 1836, 1845, 1893, 2499, 2759
Sau96I GGNCC 2 cut(s) 900, 1909
ScaI AGTACT 1 cut(s) 2125
SchI GAGTC 8 cut(s) 746, 767, 1265, 1445, 1550, 2360, 2380, 2398
ScrFI CCNGG 4 cut(s) 626, 646, 1985, 2136
SduI GDGCHC 2 cut(s) 185, 2694
SfaNI GCATC 8 cut(s) 103, 183, 275, 1148, 1202, 1630, 1656, 1691
SfcI CTRYAG 4 cut(s) 72, 1299, 1395, 1479
Sfr274I CTCGAG 1 cut(s) 2502
SinI GGWCC 1 cut(s) 900
SlaI CTCGAG 1 cut(s) 2502
SmiMI CAYNNNNRTG 2 cut(s) 82, 1360
SmlI CTYRAG 5 cut(s) 184, 1921, 2502, 2666, 2687
SmoI CTYRAG 5 cut(s) 184, 1921, 2502, 2666, 2687
SpeI ACTAGT 1 cut(s) 671
SsiI CCGC 4 cut(s) 146, 916, 2720, 2770
SspI AATATT 2 cut(s) 580, 968
StyD4I CCNGG 4 cut(s) 624, 644, 1983, 2134
StyI CCWWGG 4 cut(s) 509, 932, 2408, 2575
TaiI ACGT 4 cut(s) 520, 595, 838, 1080
TaqI TCGA 6 cut(s) 111, 619, 1158, 1601, 2503, 2740
TaqII GACCGA 1 cut(s) 888
TatI WGTACW 4 cut(s) 1096, 1803, 2123, 2253
TscAI CASTG 8 cut(s) 123, 730, 805, 1074, 1099, 1732, 1968, 2161
TseFI GTSAC 4 cut(s) 708, 918, 1086, 1353
TseI GCWGC 9 cut(s) 5, 74, 243, 1832, 1835, 1844, 1892, 2498, 2758
Tsp45I GTSAC 4 cut(s) 708, 918, 1086, 1353
TspGWI ACGGA 3 cut(s) 992, 1203, 2502
TspRI CASTG 8 cut(s) 123, 730, 805, 1074, 1099, 1732, 1968, 2161
Van91I CCANNNNNTGG 1 cut(s) 869
Vha464I CTTAAG 1 cut(s) 1921
VpaK11BI GGWCC 1 cut(s) 900
VspI ATTAAT 2 cut(s) 1113, 2559
XapI RAATTY 4 cut(s) 40, 986, 2045, 2298
XbaI TCTAGA 1 cut(s) 2230
XceI RCATGY 3 cut(s) 81, 716, 2698
XcmI CCANNNNNNNNNTGG 1 cut(s) 1981
XhoI CTCGAG 1 cut(s) 2502
XmaJI CCTAGG 1 cut(s) 2408
ZraI GACGTC 1 cut(s) 1078
ZrmI AGTACT 1 cut(s) 2125
Zsp2I ATGCAT 2 cut(s) 363, 1793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.