Rorug06G0201100

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003313
Physical Location & Seq
Forward (+)
32291069 .. 32295224
4156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0201100.1

Sequence Viewer

Length: 2916 bp
ATGGCTGAAGCACTCATTTTCGTTCTCCTAGAACAATTGGCTTCAATCACCCGTCAACAGGTACAACTACAAGTGAAATTGGTAGTTGATGTTAAGAAAGAAGTTGAAAATCTCCGCCACAATTTCCGAGCTATAGAAGCCGTGCTCAAAGATGCAGAGGAGAGGCAAGTGAAGGAAGCTTCCATTAAATTATGGCTGGATGATTTAAAGGATGTGTCCAACGAGATGGAGGACGTGTTGGATGATTGGAACACTGAAATTCTGAGGCTACAAATTGAGAAACAAGAGAAAGAAGCGGGAAATGCTCTTGAAACGACTAAGAAGAAGGTATGTCTATGCATTCCTACTGCTTTCTTTTGTTTTGGCCAAGTTAGTAGAGTTATTTTTCGTCGAGACATTGCTCAAAAGATTAAAGATCTCAGTGAAAGGTTAGCAACAATTGCTAATATGAGGCAAAATTTTAACTTTCAGTACACAAAAAGTGGTACTGGACATATTGAGCGACAAAAGACCACTTCTTTTGTCGATCAAACATTTGGTCGGGTAGATGAAAAAAAATTCTTGGTGGGCATGTTGTTGAGTGAGAGTAATCCTGAAGGGCAAAGCCCCATTGTCATCCCGATTGTAGGGATGGGTGGAATCGGTAAAACAACTCTTTCGCAACTAGCATATAATGACGAAAAAGTGAAGGCTAATTTCACAAAAAGAATATGGGTTTGTGTCTCAGACCCATTTGATGAGCTCAACATAGCCAAAGCCATCATTAGAGCCCTTGATGGAAATGTCAATTGCATTTCAAATGACCTAGAAGCTTTCTTCCAATGTGTGCACAAGTCTATCGAGGGGAAAAAGTTTCTCCTTGTCTTAGATGATGTGTGGAACCAAGACTATAGGAAGTGGGAAAATTTGAAGCTACCTTTACATAATGGTGCTCTAGGTAGTAAAATAATAGTCACTACACGAAAAGAAGAAGTAGCTAGGATGATGGGAGCACCCGATAACACAATCAATTTGAAGGTGTTGAGTGAAGAAAATTGTTGGTCACTGTTCACTAGAATTGCCTTTACCAATAGAAACCAAGATGAGTGTCAAATTTTGGAACCTATTGGTAAGGAAATTGTAAAGAAGTGCAAAGGGTTGCCACTTATTGCCAAGACTTTAGGTGGTCTCATGCGTTGTAAGAAAACAAAGAAAGAATGGCAAGATGTTTTGGGTAGTGAGATATGGGAGTTAGACATAATGGAGCAACAAGTTTTCCAACCGTTGTTACTAAGTTATTATGATTTAACTTCAATGATCAAGCGTTGTCTCTTATATTGTGTTACCTTCCCAAAAAATCATATAATTGACAAAGAAGTTTTGATTGAGTTGTGGATGTCGCAAGGTTTTCTTGGTTCATTTGCAAACAAAGAAAAGACAATGATAAACATTGGAGAATCATATTTCGATAACTTGGTAATGCGATCTTTCTTCCAGAATTTGAAGAAAGATGAATTTGGAGATATTGAACATTGCCAAATGCATGATATTGTGCACGACTTTCTCCAATATTTAACCAAGAATGAATGTTTAATCGTTGAGAAAAAAGGTGTTGAGGAGAGAATAGAATTTCCAGCAGACAATAAGCTCCGTCATTTAACCATAATGTTTGCACCGATAGGACGTTTTCCCCTTTCTTTTGACCATTGCCAACGTCTACGGACCATTACAACATTTAATTGTAAAATTACAAGTTTTACGCGAGAGTTGATTTTACAATTAAAATGCGTGAGGACGTTGAATTTGAGTGACAACTATATCGAAGAAGTTCCGGACGAGGTTGGCGGGTTGGTGCACTTGAGGTATTTGGATTTATCTCGTAATCGGAGGCTGAAGAAATTACCTGATAGTCTGTGTAATTTAGTCAATTTGCAAACCTTGCGAGTTGATTGGTGCATTAGCCTTGAAAAGTTCCCCGAGGCCATGGGAAAGTTGACCAGCTTGCAGCATCTTCATGCTAATCTTGGTTTTTGCGACATTGATAAACTGCTGAAGCTGCCCAAATCAATTGCGATGTTGAAAAGTCTACGGACGCTAGATGTTGAACCTGTGTTATTAATTGAAGGTGAAGGTGAAGATGGAGATGAAGAAGGATTGAGGTCATGTGATTTGAGAGACATGGACGAGCTTCAGGGGAAACTTTGGATCGTATTAAAGGGCAAATTGAAAGATAGTGCTGCGTGTGATGTGGAGAAGGCCAAATTGGTGAATAAGAAACACCTTCTTCATTTGAAACTAGGGTTCGCCTGTGAAAGCGAGGGCAACCAGAGTACCATCGAGGAAGCAGTATTGAATGTCTTACAACCAAATTCAAATCTTGAATCCTTATGGATCAGAGGTTACGATGGCAGCACCCTGTACCCCAATTGGACCTCCTCTTTAATTAACTTGAAAAGCCTAACCTTCTCTTGGTGCTTGGTTTGTAACTATGTGCCTCTTTCGGTTTTGGGCAACTTGGGGTCCCTTGAGAGACTCAGATTTGATGACATGCCACAAGTGAAAAAGGTTGGATTTGAGTACTCACTTGATCATCAAGTTATATTGTTCCCCAATCTGAAACAACTCGAGTTCGCTGAAATGCCGGAGTGGGAAGAATGGGATTATCATGAGAATGATGCTACTTCATCGTCACCAACTTTTTTCATGCCACGCCTTTCTACTTTATCCATTGAATGGTGCTATAAGCTGAAGACATTGCCAGACTTCCTTCCAAGGAAGACGCCGCTGCTTCAGAAGTTGATCATCAACGATTGTCCCATTGTCTCCAAAAGTTGCAAAGACATGCAAGGCCCGGAGTGTTCCAAGATTTCTCACATCCCAAACATCCATATTGAATGGAAAACTATACAAAAAGATGGAGTTTGGATCAAACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

971

Amino Acids

111.73

Weight (kDa)

6.18

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 95 1.2e-20 Rx N-terminal domain
NB-ARC PF00931 192 - 358 4.7e-38 NB-ARC domain
WHD_DRP PF23559 443 - 514 2.9e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 566 - 904 2.5e-21 Leucine-rich repeat region
LRR_8 PF13855 590 - 644 2.6e-08 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 716 - 843 7.6e-25 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 2712
AccI GTMKAC 2 cut(s) 1696, 2065
AccII CGCG 1 cut(s) 1741
AccIII TCCGGA 1 cut(s) 1810
AciI CCGC 4 cut(s) 115, 296, 1824, 2762
AclWI GGATC 3 cut(s) 2192, 2378, 2912
AcoI YGGCCR 1 cut(s) 364
AcuI CTGAAG 7 cut(s) 27, 615, 1892, 2051, 2153, 2747, 2753
AcyI GRCGYC 1 cut(s) 2759
AfaI GTAC 6 cut(s) 63, 473, 487, 2311, 2399, 2558
AfiI CCNNNNNNNGG 4 cut(s) 58, 626, 2448, 2712
AflIII ACRYGT 1 cut(s) 234
AjiI CACGTC 1 cut(s) 235
AjuI GAANNNNNNNTTGG 2 cut(s) 2225, 2257
Alw21I GWGCWC 7 cut(s) 147, 744, 831, 934, 994, 1536, 1836
Alw26I GTCTC 7 cut(s) 387, 727, 1172, 1313, 2148, 2503, 2806
Alw44I GTGCAC 3 cut(s) 827, 1532, 1832
AlwI GGATC 3 cut(s) 2192, 2378, 2912
Ama87I CYCGRG 2 cut(s) 1955, 2603
Aor13HI TCCGGA 1 cut(s) 1810
AoxI GGCC 4 cut(s) 364, 1959, 2235, 2827
ApaLI GTGCAC 3 cut(s) 827, 1532, 1832
ApeKI GCWGC 5 cut(s) 1984, 2035, 2216, 2388, 2764
ArsI GACNNNNNNTTYG 4 cut(s) 523, 555, 1765, 1797
AseI ATTAAT 1 cut(s) 2096
Asp700I GAANNNNTTC 1 cut(s) 1806
AspS9I GGNCC 4 cut(s) 1701, 2409, 2499, 2828
AsuC2I CCSGG 1 cut(s) 2831
AsuHPI GGTGA 5 cut(s) 40, 2117, 2123, 2257, 2661
AvaI CYCGRG 2 cut(s) 1955, 2603
AvaII GGWCC 3 cut(s) 1701, 2409, 2499
BaeGI GKGCMC 3 cut(s) 831, 1536, 1836
BaeI ACNNNNGTAYC 2 cut(s) 2293, 2326
BalI TGGCCA 1 cut(s) 366
BanII GRGCYC 2 cut(s) 744, 772
BbsI GAAGAC 2 cut(s) 2735, 2762
Bbv12I GWGCWC 7 cut(s) 147, 744, 831, 934, 994, 1536, 1836
BbvI GCAGC 5 cut(s) 1996, 2022, 2203, 2400, 2751
BccI CCATC 9 cut(s) 220, 625, 767, 770, 979, 2111, 2321, 2378, 2888
BceAI ACGGC 1 cut(s) 125
BclI TGATCA 3 cut(s) 1296, 2566, 2778
BcnI CCSGG 1 cut(s) 2831
BcoDI GTCTC 7 cut(s) 387, 727, 1172, 1313, 2148, 2503, 2806
BfaI CTAG 8 cut(s) 29, 665, 806, 935, 978, 1053, 2075, 2276
BfmI CTRYAG 2 cut(s) 132, 889
BglII AGATCT 1 cut(s) 415
BisI GCNGC 6 cut(s) 1985, 2036, 2217, 2389, 2762, 2765
BlsI GCNGC 6 cut(s) 1986, 2037, 2218, 2390, 2763, 2766
BmcAI AGTACT 1 cut(s) 2558
Bme1390I CCNGG 1 cut(s) 2831
Bme18I GGWCC 3 cut(s) 1701, 2409, 2499
BmeT110I CYCGRG 2 cut(s) 1955, 2603
BmgBI CACGTC 1 cut(s) 235
BmgT120I GGNCC 4 cut(s) 1701, 2409, 2499, 2828
BmiI GGNNCC 4 cut(s) 881, 1101, 2500, 2501
BmrFI CCNGG 1 cut(s) 2831
BmsI GCATC 3 cut(s) 142, 1996, 2644
BpiI GAAGAC 2 cut(s) 2735, 2762
BpuEI CTTGAG 2 cut(s) 1858, 2525
BpuMI CCSGG 1 cut(s) 2831
BsaHI GRCGYC 1 cut(s) 2759
BsaI GGTCTC 1 cut(s) 1172
BsaJI CCNNGG 3 cut(s) 1956, 1962, 2750
BsaWI WCCGGW 1 cut(s) 1810
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 626, 2448, 2712
Bse1I ACTGG 1 cut(s) 493
Bse3DI GCAATG 4 cut(s) 396, 1510, 1684, 2732
BseAI TCCGGA 1 cut(s) 1810
BseDI CCNNGG 3 cut(s) 1956, 1962, 2750
BseGI GGATG 9 cut(s) 205, 217, 247, 615, 636, 987, 1380, 2853, 2862
BseLI CCNNNNNNNGG 4 cut(s) 58, 626, 2448, 2712
BseMI GCAATG 4 cut(s) 396, 1510, 1684, 2732
BseMII CTCAG 4 cut(s) 254, 433, 738, 2527
BseNI ACTGG 1 cut(s) 493
BseRI GAGGAG 3 cut(s) 173, 1610, 2404
BseSI GKGCMC 3 cut(s) 831, 1536, 1836
BseXI GCAGC 5 cut(s) 1996, 2022, 2203, 2400, 2751
Bsh1236I CGCG 1 cut(s) 1741
BshFI GGCC 4 cut(s) 366, 1961, 2237, 2829
BsiHKAI GWGCWC 7 cut(s) 147, 744, 831, 934, 994, 1536, 1836
BsiHKCI CYCGRG 2 cut(s) 1955, 2603
BsiSI CCGG 3 cut(s) 1811, 2621, 2831
BslFI GGGAC 2 cut(s) 2485, 2778
BslI CCNNNNNNNGG 4 cut(s) 58, 626, 2448, 2712
BsmAI GTCTC 7 cut(s) 387, 727, 1172, 1313, 2148, 2503, 2806
BsmFI GGGAC 2 cut(s) 2485, 2778
BsmI GAATGC 1 cut(s) 339
BsnI GGCC 4 cut(s) 366, 1961, 2237, 2829
Bso31I GGTCTC 1 cut(s) 1172
BsoBI CYCGRG 2 cut(s) 1955, 2603
Bsp1286I GDGCHC 8 cut(s) 147, 744, 772, 831, 934, 994, 1536, 1836
Bsp13I TCCGGA 1 cut(s) 1810
Bsp143I GATC 9 cut(s) 415, 526, 1296, 1463, 2184, 2370, 2566, 2778, 2904
Bsp19I CCATGG 1 cut(s) 1962
BspACI CCGC 4 cut(s) 115, 296, 1824, 2762
BspANI GGCC 4 cut(s) 366, 1961, 2237, 2829
BspCNI CTCAG 4 cut(s) 255, 432, 737, 2526
BspEI TCCGGA 1 cut(s) 1810
BspFNI CGCG 1 cut(s) 1741
BspHI TCATGA 1 cut(s) 2644
BspLI GGNNCC 4 cut(s) 881, 1101, 2500, 2501
BspPI GGATC 3 cut(s) 2192, 2378, 2912
BspTNI GGTCTC 1 cut(s) 1172
BsrDI GCAATG 4 cut(s) 396, 1510, 1684, 2732
BsrI ACTGG 1 cut(s) 493
BssECI CCNNGG 3 cut(s) 1956, 1962, 2750
BssMI GATC 9 cut(s) 415, 526, 1296, 1463, 2184, 2370, 2566, 2778, 2904
BssNI GRCGYC 1 cut(s) 2759
BssT1I CCWWGG 2 cut(s) 1962, 2750
Bst4CI ACNGT 3 cut(s) 1047, 1263, 2913
BstACI GRCGYC 1 cut(s) 2759
BstAPI GCANNNNNTGC 2 cut(s) 440, 1918
BstC8I GCNNGC 1 cut(s) 1982
BstDEI CTNAG 7 cut(s) 263, 318, 419, 724, 865, 1271, 2513
BstDSI CCRYGG 1 cut(s) 1962
BstF5I GGATG 9 cut(s) 205, 217, 247, 615, 636, 987, 1380, 2853, 2862
BstFNI CGCG 1 cut(s) 1741
BstKTI GATC 9 cut(s) 418, 529, 1299, 1466, 2187, 2373, 2569, 2781, 2907
BstMAI GTCTC 7 cut(s) 387, 727, 1172, 1313, 2148, 2503, 2806
BstMBI GATC 9 cut(s) 415, 526, 1296, 1463, 2184, 2370, 2566, 2778, 2904
BstMWI GCNNNNNNNGC 5 cut(s) 137, 302, 440, 1918, 2035
BstNSI RCATGY 3 cut(s) 574, 2530, 2824
BstSCI CCNGG 1 cut(s) 2829
BstSFI CTRYAG 2 cut(s) 132, 889
BstSLI GKGCMC 3 cut(s) 831, 1536, 1836
BstUI CGCG 1 cut(s) 1741
BstV1I GCAGC 5 cut(s) 1996, 2022, 2203, 2400, 2751
BstV2I GAAGAC 2 cut(s) 2735, 2762
BstX2I RGATCY 1 cut(s) 415
BstXI CCANNNNNNTGG 1 cut(s) 226
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 4 cut(s) 366, 1961, 2237, 2829
BtgI CCRYGG 1 cut(s) 1962
BtgZI GCGATG 1 cut(s) 2066
BtrI CACGTC 1 cut(s) 235
BtsCI GGATG 9 cut(s) 205, 217, 247, 615, 636, 987, 1380, 2853, 2862
BtsIMutI CAGTG 3 cut(s) 252, 427, 1043
Cac8I GCNNGC 1 cut(s) 1982
CciI TCATGA 1 cut(s) 2644
Cfr13I GGNCC 4 cut(s) 1701, 2409, 2499, 2828
CseI GACGC 2 cut(s) 2080, 2767
Csp6I GTAC 6 cut(s) 62, 472, 486, 2310, 2398, 2557
CspCI CAANNNNNGTGG 2 cut(s) 502, 537
CviQI GTAC 6 cut(s) 62, 472, 486, 2310, 2398, 2557
DdeI CTNAG 7 cut(s) 263, 318, 419, 724, 865, 1271, 2513
DpnI GATC 9 cut(s) 417, 528, 1298, 1465, 2186, 2372, 2568, 2780, 2906
DpnII GATC 9 cut(s) 415, 526, 1296, 1463, 2184, 2370, 2566, 2778, 2904
DraI TTTAAA 1 cut(s) 207
EaeI YGGCCR 1 cut(s) 364
EciI GGCGGA 1 cut(s) 104
Ecl136II GAGCTC 1 cut(s) 742
Eco130I CCWWGG 2 cut(s) 1962, 2750
Eco24I GRGCYC 2 cut(s) 744, 772
Eco31I GGTCTC 1 cut(s) 1172
Eco47I GGWCC 3 cut(s) 1701, 2409, 2499
Eco53kI GAGCTC 1 cut(s) 742
Eco57I CTGAAG 7 cut(s) 27, 615, 1892, 2051, 2153, 2747, 2753
Eco88I CYCGRG 2 cut(s) 1955, 2603
EcoICRI GAGCTC 1 cut(s) 742
EcoO109I RGGNCCY 1 cut(s) 2499
EcoT14I CCWWGG 2 cut(s) 1962, 2750
EcoT22I ATGCAT 2 cut(s) 341, 1524
EcoT38I GRGCYC 2 cut(s) 744, 772
ErhI CCWWGG 2 cut(s) 1962, 2750
FalI AAGNNNNNCTT 2 cut(s) 1374, 1406
FaqI GGGAC 2 cut(s) 2485, 2778
FauI CCCGC 2 cut(s) 289, 1817
FbaI TGATCA 3 cut(s) 1296, 2566, 2778
FblI GTMKAC 2 cut(s) 1696, 2065
Fnu4HI GCNGC 6 cut(s) 1985, 2036, 2217, 2389, 2762, 2765
FokI GGATG 9 cut(s) 212, 224, 254, 602, 643, 994, 1387, 2840, 2849
FriOI GRGCYC 2 cut(s) 744, 772
Fsp4HI GCNGC 6 cut(s) 1985, 2036, 2217, 2389, 2762, 2765
FspBI CTAG 8 cut(s) 29, 665, 806, 935, 978, 1053, 2075, 2276
GluI GCNGC 6 cut(s) 1985, 2036, 2217, 2389, 2762, 2765
HaeIII GGCC 4 cut(s) 366, 1961, 2237, 2829
HapII CCGG 3 cut(s) 1811, 2621, 2831
HgaI GACGC 2 cut(s) 2080, 2767
Hin1I GRCGYC 1 cut(s) 2759
HincII GTYRAC 2 cut(s) 56, 1974
HindII GTYRAC 2 cut(s) 56, 1974
HindIII AAGCTT 2 cut(s) 177, 810
HinfI GANTC 4 cut(s) 639, 1436, 2360, 2511
HpaII CCGG 3 cut(s) 1811, 2621, 2831
HphI GGTGA 5 cut(s) 40, 2117, 2123, 2257, 2661
Hpy166II GTNNAC 9 cut(s) 56, 474, 829, 1050, 1534, 1697, 1834, 1974, 2066
Hpy188I TCNGA 8 cut(s) 128, 264, 727, 1866, 2375, 2516, 2595, 2772
Hpy188III TCNNGA 8 cut(s) 308, 392, 593, 619, 1474, 1811, 2357, 2645
Hpy8I GTNNAC 9 cut(s) 56, 474, 829, 1050, 1534, 1697, 1834, 1974, 2066
Hpy99I CGWCG 1 cut(s) 393
HpyCH4III ACNGT 3 cut(s) 1047, 1263, 2913
HpyCH4IV ACGT 4 cut(s) 234, 1663, 1693, 1775
HpyF10VI GCNNNNNNNGC 5 cut(s) 137, 302, 440, 1918, 2035
HpyF3I CTNAG 7 cut(s) 263, 318, 419, 724, 865, 1271, 2513
HpySE526I ACGT 4 cut(s) 234, 1663, 1693, 1775
Hsp92I GRCGYC 1 cut(s) 2759
KflI GGGWCCC 1 cut(s) 2499
Kpn2I TCCGGA 1 cut(s) 1810
Ksp22I TGATCA 3 cut(s) 1296, 2566, 2778
Kzo9I GATC 9 cut(s) 415, 526, 1296, 1463, 2184, 2370, 2566, 2778, 2904
LmnI GCTCC 3 cut(s) 989, 1243, 1632
Lsp1109I GCAGC 5 cut(s) 1996, 2022, 2203, 2400, 2751
LweI GCATC 3 cut(s) 142, 1996, 2644
MaeI CTAG 8 cut(s) 29, 665, 806, 935, 978, 1053, 2075, 2276
MaeII ACGT 4 cut(s) 234, 1663, 1693, 1775
MaeIII GTNAC 8 cut(s) 952, 1041, 1266, 1321, 1787, 2378, 2462, 2667
MalI GATC 9 cut(s) 417, 528, 1298, 1465, 2186, 2372, 2568, 2780, 2906
MboI GATC 9 cut(s) 415, 526, 1296, 1463, 2184, 2370, 2566, 2778, 2904
MfeI CAATTG 5 cut(s) 35, 438, 787, 2046, 2404
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 8 cut(s) 147, 744, 772, 831, 934, 994, 1536, 1836
MlsI TGGCCA 1 cut(s) 366
MluNI TGGCCA 1 cut(s) 366
MlyI GAGTC 1 cut(s) 2505
MmeI TCCRAC 4 cut(s) 219, 243, 1282, 2527
Mox20I TGGCCA 1 cut(s) 366
Mph1103I ATGCAT 2 cut(s) 341, 1524
MroI TCCGGA 1 cut(s) 1810
MroXI GAANNNNTTC 1 cut(s) 1806
MscI TGGCCA 1 cut(s) 366
MslI CAYNNNNRTG 3 cut(s) 927, 1992, 2649
Msp20I TGGCCA 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 2764
MspI CCGG 3 cut(s) 1811, 2621, 2831
MspR9I CCNGG 1 cut(s) 2831
MunI CAATTG 5 cut(s) 35, 438, 787, 2046, 2404
Mva1269I GAATGC 1 cut(s) 339
MvnI CGCG 1 cut(s) 1741
MwoI GCNNNNNNNGC 5 cut(s) 137, 302, 440, 1918, 2035
NciI CCSGG 1 cut(s) 2831
NcoI CCATGG 1 cut(s) 1962
NdeII GATC 9 cut(s) 415, 526, 1296, 1463, 2184, 2370, 2566, 2778, 2904
NlaIV GGNNCC 4 cut(s) 881, 1101, 2500, 2501
NmuCI GTSAC 4 cut(s) 952, 1041, 1787, 2667
NsiI ATGCAT 2 cut(s) 341, 1524
NspI RCATGY 3 cut(s) 574, 2530, 2824
PacI TTAATTAA 1 cut(s) 2424
PaeR7I CTCGAG 1 cut(s) 2603
PagI TCATGA 1 cut(s) 2644
PctI GAATGC 1 cut(s) 339
PdmI GAANNNNTTC 1 cut(s) 1806
PfeI GAWTC 3 cut(s) 639, 1436, 2360
PflMI CCANNNNNTGG 1 cut(s) 2712
PkrI GCNGC 6 cut(s) 1986, 2037, 2218, 2390, 2763, 2766
PleI GAGTC 1 cut(s) 2505
PpsI GAGTC 1 cut(s) 2505
PpuMI RGGWCCY 1 cut(s) 2499
PshBI ATTAAT 1 cut(s) 2096
Psp124BI GAGCTC 1 cut(s) 744
Psp5II RGGWCCY 1 cut(s) 2499
PspN4I GGNNCC 4 cut(s) 881, 1101, 2500, 2501
PspPI GGNCC 4 cut(s) 1701, 2409, 2499, 2828
PspPPI RGGWCCY 1 cut(s) 2499
PspXI VCTCGAGB 1 cut(s) 2603
PsuI RGATCY 1 cut(s) 415
RsaI GTAC 6 cut(s) 63, 473, 487, 2311, 2399, 2558
RsaNI GTAC 6 cut(s) 62, 472, 486, 2310, 2398, 2557
RseI CAYNNNNRTG 3 cut(s) 927, 1992, 2649
SacI GAGCTC 1 cut(s) 744
SatI GCNGC 6 cut(s) 1985, 2036, 2217, 2389, 2762, 2765
Sau3AI GATC 9 cut(s) 415, 526, 1296, 1463, 2184, 2370, 2566, 2778, 2904
Sau96I GGNCC 4 cut(s) 1701, 2409, 2499, 2828
ScaI AGTACT 1 cut(s) 2558
SchI GAGTC 1 cut(s) 2505
ScrFI CCNGG 1 cut(s) 2831
SduI GDGCHC 8 cut(s) 147, 744, 772, 831, 934, 994, 1536, 1836
SfaNI GCATC 3 cut(s) 142, 1996, 2644
SfcI CTRYAG 2 cut(s) 132, 889
Sfr274I CTCGAG 1 cut(s) 2603
SinI GGWCC 3 cut(s) 1701, 2409, 2499
SlaI CTCGAG 1 cut(s) 2603
SmiMI CAYNNNNRTG 3 cut(s) 927, 1992, 2649
SmlI CTYRAG 3 cut(s) 1837, 2504, 2603
SmoI CTYRAG 3 cut(s) 1837, 2504, 2603
SsiI CCGC 4 cut(s) 115, 296, 1824, 2762
SspI AATATT 1 cut(s) 1550
SspMI CTAG 8 cut(s) 29, 665, 806, 935, 978, 1053, 2075, 2276
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 1 cut(s) 2829
StyI CCWWGG 2 cut(s) 1962, 2750
TaaI ACNGT 3 cut(s) 1047, 1263, 2913
TaiI ACGT 4 cut(s) 237, 1666, 1696, 1778
TaqI TCGA 7 cut(s) 391, 525, 840, 1446, 1800, 2316, 2604
TatI WGTACW 2 cut(s) 471, 2556
TauI GCSGC 1 cut(s) 2764
TfiI GAWTC 3 cut(s) 639, 1436, 2360
TscAI CASTG 3 cut(s) 259, 427, 1050
TseFI GTSAC 4 cut(s) 952, 1041, 1787, 2667
TseI GCWGC 5 cut(s) 1984, 2035, 2216, 2388, 2764
Tsp45I GTSAC 4 cut(s) 952, 1041, 1787, 2667
TspDTI ATGAA 9 cut(s) 564, 1386, 1506, 1578, 1982, 2139, 2255, 2652, 2671
TspGWI ACGGA 3 cut(s) 1619, 1714, 2083
TspRI CASTG 3 cut(s) 259, 427, 1050
Van91I CCANNNNNTGG 1 cut(s) 2712
VneI GTGCAC 3 cut(s) 827, 1532, 1832
VpaK11BI GGWCC 3 cut(s) 1701, 2409, 2499
VspI ATTAAT 1 cut(s) 2096
XceI RCATGY 3 cut(s) 574, 2530, 2824
XhoI CTCGAG 1 cut(s) 2603
XmiI GTMKAC 2 cut(s) 1696, 2065
XmnI GAANNNNTTC 1 cut(s) 1806
XspI CTAG 8 cut(s) 29, 665, 806, 935, 978, 1053, 2075, 2276
ZrmI AGTACT 1 cut(s) 2558
Zsp2I ATGCAT 2 cut(s) 341, 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.