MD10G1190500.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003313
Physical Location & Seq
Reverse (-)
28764519 .. 28768185
3667 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1190500.v1.1.491

Sequence Viewer

Length: 3027 bp
ATGGCAGCAAAGGGAAATTCATCTGTACATGTGCATAAGAACTTAGAGGGTTTTGTAAACATTCTAACATCTGCAGCATGTGAGTGGGTTTTGATCTTTCTTCTGCTTGTCGATGGACTATTATCCTATCTGCTTACTAAATTTGCAGAATATTGCAAGTTGCAAAAGCCTTGCATCTTTTGCTCCAGGTTTGAACATGTTATAGGCAGTGAAAAACCAAAACCTCAACTTTATCAGGATCTACTTTGCAGCAACCACATATCTGAGTTCTCGTCATGGTTGTCTTGTCAAATTTATGGTAAGCATGATGGCAATGGAATGTGTGAAGATTGCATCGTGTCATATGCCAAAAAGGGCAAGTCAAACCCCGAAAAGCACATGTTTTCGGCTGGCAAGTCGGATTCTGATATTGGAAGTTCCGGTTTTAGGAGGTCCATGCAGAACAGAAAGTTTGTTCATAGTTCTGTGGGTGTAAGGCCATGCTCTTGTTGCAGTAGGCCTTATATACCTAGAGAAAATGCTCGAGGACTACAACGAAAATCATCTGCTTCCAAACCTAATATTCCTTTGCCACGTTTTTCAAGCCGTAGTCGTATACCTCGCAGGGATGGTTCAAAGAAGACCAGGGATAAAATTTCTGGATCAGTAACTACTCGTCATCCTGGAAAACTTGGCTGGGATCCCTTGCCACATAGAGGACGCAAGTTGAGGATTAGTTCTGATACTGACTCCACTCTTCGTCCTGGTAAATTTGGCCGGGATCCCTTGCCACATGTAGGATACTCTGAGTTGAGGATTAATTCCGATACTGAATCCGCTCGTCGTGCTGGAAAACTTGGCTGGGATCCCTTGTCTCATGTAGGATACACTGAGTTGAAGATTAGTTCTGATACCGATTCTGAGATTCCATTTTCTGATGAAGACGGTGGTAGCAGCATAATCATTGGAAATCAGGAGCATAAGAAGGAAGCTTTAGTAGCTCAATATACTTCAGAAAAACCATTCAAAGCACCGCTAAATGGTTTGAATCCAGCAAAGCAGAGCAACGTTACCACCGACCACAAGCCTAGACTTTCTGATCCAAGTGTGCAGCCAGATGGTAGCACGAGGTCTGATATAAAATTCTTAGGCTCGAATGTTGCTTCAAAGAATGGCTTGGGTGAGCTTAACTGGCAGCAAGCGAGTCAGAATTCCATCCCTTATGCATTCCCTGAGCTTATTTCACTAGATGATATCCCTCCATCATTTAATGTTGCTGAAACTTGTTTTACTGGGACAAGCGACTTTGGAGATGCACCCATCAGTAAGAATGATGAACTTTTGAAGTCTATAAGTGCAACAGATGGAGCACCTATTAAAACCGATCCTATCATCAATGATCCGCCTCTTACGAAGCCAAATCATATTAATGTAAATGCTGTGTCTGAGTCAGCAGGCATTGGTAAGGAAGGAGATACATCCAATTCTATTAAAGAACAACCTACAGTGAAGGTACCTGATAGAGTTGATGGGGAAGTGAAACCAACGGACTCACAAAATTGTTCAGCTCAGGAGAGAAATTTATCATCAAATAATACAGTTTCAGGGGTACTTGGTGTTGGTGATGAAAATCCGGTAACTGATGCTTCCAAATCGTCTGCTCAGGAGAGTAATTTATCGTCAAATAGTACAATTTCAGGGGTACATGGTGTTGGTGATGAAAAGCCGATTAGTGATGCTTCCAAATCTTCTGCTCAGGGGAGAAATTTATCATTGAATGGCACAATTTCAGCGGCACGTGGCGTTGGTGACGAAAAGCCCGTACCTGATGCTTCAAAGTCTTCTGCTCGGAAGAGTAATTCATCATCAAATAATACAATTTCAGGGGTATGTGGTGGTGGTGATGAAAAACAGGCAACTGATGCTTCCAATTCTAATCCAAATCCAGGGCTTAAAACATCATGGTCAGTAGAATCTGGTCTTGAATCTCTGGATGAAGGATATGTCAGTGAAATTGAAGGTGAAAGTATTGTCGATCGGTTAAAACGACAGGTTGATTATTATAGAAAGTGCATAAAGGAGTTGTTTAGGGAATTAGACGAAGAAAGGAATGCCGCTGAAATTGCTGCAAATCAGGCAATGGCCATGATCACAAAGCTACAGGAAGAGAAAGCTGCAATCCATATGGAGGCCCTTCAGTATTTAAGAATGATGGAAGAGCAGGCTGAGTTTGATGTAGATGCACTCGAAAAAGCCAATGATCTCCTGGTTGAAAAGGAGAAAGAAATACAGGACTTGGAAGCAGAGCTAGAATTTTCTAAGTTCGATATCCCAGATGAATCGACAATGGAGAGTATGCCAATGCCGGCATTGCCGGTAAAAAGTTATGATTTGAAGGGAAAGATTGACACAGTGGAGAGTACTGTGGTACCTGAAGCCGTTGTTCCCTGCGTAAAAAGTGATGTCGAAGCTACTGGTAACTCAACAGTTACTGAGGTACCTAAAGCTAGCAATGAACGCCTTTTTTCTGAGACAACACTTTTGGAATTTGAAGATGAAAAATTGTACATTTCAAATTGTTTGAAGAGCTTGGAGAGGAAGCTTTCCGAAATTTCCTGCAATGGGGCTTCCGCCAACATGCCTGATGGTGGGCATTCTAGAAAGCATACAGATGATGAACCGAATCAAGTAGAGACGCCTAAAAAATTTAGGCTTTTATTAAATGGTCAGATTGAAGAGGAAGTTGTGCAGATGCCAAACGATTTACATATATCTAACGGAAGCGCTGCTCCACAAGAGGGACCAATTCATTGCGATAGTTTAGACCGTGAAGACAAAAAAGATTCTGAATTGGATGTTCTGGAAAACGAAATGTCAGACCTTAACGACAGGTTGGAGGCGCTCGAGACTGATCACGATTTTCTTGAGCACATGCTTTACTCTCTTCAAAATGGACAGGAAGGGCTACAGTTTGTTCAAGAGATAGCTCAGGAGCTGCGAGAACTGCGAAAAATTGGCGCAGCTTTCAGAAATCCGTCCGCTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

1009

Amino Acids

110.27

Weight (kDa)

5.18

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 673 - 763 5.4e-29 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 3 cut(s) 1492, 2407, 2476
AccB1I GGYRCC 3 cut(s) 1492, 2407, 2476
AccBSI CCGCTC 2 cut(s) 818, 3020
AccI GTMKAC 1 cut(s) 595
AciI CCGC 7 cut(s) 816, 1013, 1382, 1772, 2094, 2610, 3018
AclI AACGTT 1 cut(s) 1047
AcoI YGGCCR 2 cut(s) 754, 2121
AcuI CTGAAG 3 cut(s) 975, 2159, 2433
AcvI CACGTG 1 cut(s) 1778
AcyI GRCGYC 1 cut(s) 2675
AfeI AGCGCT 1 cut(s) 2764
AfiI CCNNNNNNNGG 9 cut(s) 426, 695, 776, 1019, 1925, 2167, 2601, 2627, 2777
AflIII ACRYGT 4 cut(s) 28, 196, 378, 772
AjnI CCWGG 6 cut(s) 185, 623, 661, 742, 1924, 2244
Alw21I GWGCWC 2 cut(s) 1351, 2910
Alw26I GTCTC 4 cut(s) 858, 2504, 2666, 2879
AlwNI CAGNNNCTG 2 cut(s) 2471, 2974
Ama87I CYCGRG 2 cut(s) 522, 2882
Aor51HI AGCGCT 1 cut(s) 2764
AoxI GGCC 5 cut(s) 476, 497, 754, 2121, 2169
ArsI GACNNNNNNTTYG 2 cut(s) 2503, 2535
AseI ATTAAT 2 cut(s) 798, 1407
Asp718I GGTACC 3 cut(s) 1492, 2407, 2476
AspLEI GCGC 3 cut(s) 2765, 2881, 2999
AspS9I GGNCC 3 cut(s) 432, 2170, 2780
AsuC2I CCSGG 1 cut(s) 758
AsuHPI GGTGA 6 cut(s) 1172, 1613, 1706, 1799, 1892, 2012
AsuNHI GCTAGC 1 cut(s) 2486
AvaI CYCGRG 2 cut(s) 522, 2882
AvaII GGWCC 2 cut(s) 432, 2780
BaeI ACNNNNGTAYC 4 cut(s) 2391, 2424, 2460, 2493
BalI TGGCCA 1 cut(s) 2123
BamHI GGATCC 3 cut(s) 679, 760, 844
BanI GGYRCC 3 cut(s) 1492, 2407, 2476
BauI CACGAG 1 cut(s) 1105
BbrPI CACGTG 1 cut(s) 1778
BbsI GAAGAC 4 cut(s) 626, 927, 1812, 2817
Bbv12I GWGCWC 2 cut(s) 1351, 2910
BceAI ACGGC 2 cut(s) 570, 2402
BciT130I CCWGG 6 cut(s) 187, 625, 663, 744, 1926, 2246
BciVI GTATCC 2 cut(s) 773, 857
BclI TGATCA 2 cut(s) 2127, 2890
BcnI CCSGG 1 cut(s) 758
BcoDI GTCTC 4 cut(s) 858, 2504, 2666, 2879
BfaI CTAG 6 cut(s) 510, 1068, 1226, 2288, 2487, 2637
BfmI CTRYAG 4 cut(s) 72, 1482, 2138, 2945
BfoI RGCGCY 2 cut(s) 2766, 2882
BfuI GTATCC 2 cut(s) 773, 857
BmcAI AGTACT 1 cut(s) 2401
Bme1390I CCNGG 7 cut(s) 187, 625, 663, 744, 758, 1926, 2246
Bme18I GGWCC 2 cut(s) 432, 2780
BmeT110I CYCGRG 2 cut(s) 522, 2882
BmgT120I GGNCC 3 cut(s) 432, 2170, 2780
BmiI GGNNCC 7 cut(s) 681, 762, 846, 1494, 2409, 2478, 2781
BmrFI CCNGG 7 cut(s) 187, 625, 663, 744, 758, 1926, 2246
BmrI ACTGGG 1 cut(s) 1281
BmsI GCATC 9 cut(s) 183, 342, 1282, 1612, 1705, 1798, 1891, 2209, 2721
BmtI GCTAGC 1 cut(s) 2490
BmuI ACTGGG 1 cut(s) 1281
BpiI GAAGAC 4 cut(s) 626, 927, 1812, 2817
BpmI CTGGAG 1 cut(s) 169
Bpu10I CCTNAGC 5 cut(s) 1212, 1548, 1641, 1734, 2967
BpuEI CTTGAG 1 cut(s) 2924
BpuMI CCSGG 1 cut(s) 758
BsaAI YACGTR 1 cut(s) 1778
BsaBI GATNNNNATC 1 cut(s) 1608
BsaHI GRCGYC 1 cut(s) 2675
BsaJI CCNNGG 2 cut(s) 624, 1925
BsaWI WCCGGW 2 cut(s) 419, 1612
Bsc4I CCNNNNNNNGG 9 cut(s) 426, 695, 776, 1019, 1925, 2167, 2601, 2627, 2777
Bse118I RCCGGY 2 cut(s) 2344, 2353
Bse1I ACTGG 3 cut(s) 1175, 1276, 2458
Bse3DI GCAATG 6 cut(s) 319, 2124, 2348, 2497, 2605, 2788
Bse8I GATNNNNATC 1 cut(s) 1608
BseBI CCWGG 6 cut(s) 187, 625, 663, 744, 1926, 2246
BseDI CCNNGG 2 cut(s) 624, 1925
BseGI GGATG 6 cut(s) 613, 658, 1194, 1457, 1978, 2839
BseJI GATNNNNATC 1 cut(s) 1608
BseLI CCNNNNNNNGG 9 cut(s) 426, 695, 776, 1019, 1925, 2167, 2601, 2627, 2777
BseMI GCAATG 6 cut(s) 319, 2124, 2348, 2497, 2605, 2788
BseNI ACTGG 3 cut(s) 1175, 1276, 2458
BseYI CCCAGC 2 cut(s) 675, 840
BsgI GTGCAG 2 cut(s) 1109, 2747
Bsh1285I CGRYCG 1 cut(s) 2017
BshFI GGCC 5 cut(s) 478, 499, 756, 2123, 2171
BshNI GGYRCC 3 cut(s) 1492, 2407, 2476
BsiEI CGRYCG 1 cut(s) 2017
BsiHKAI GWGCWC 2 cut(s) 1351, 2910
BsiHKCI CYCGRG 2 cut(s) 522, 2882
BsiSI CCGG 5 cut(s) 420, 757, 1613, 2345, 2354
BslFI GGGAC 2 cut(s) 1288, 2793
BslI CCNNNNNNNGG 9 cut(s) 426, 695, 776, 1019, 1925, 2167, 2601, 2627, 2777
BsmAI GTCTC 4 cut(s) 858, 2504, 2666, 2879
BsmBI CGTCTC 1 cut(s) 2666
BsmFI GGGAC 2 cut(s) 1288, 2793
BsmI GAATGC 3 cut(s) 1205, 2095, 2632
BsnI GGCC 5 cut(s) 478, 499, 756, 2123, 2171
BsoBI CYCGRG 2 cut(s) 522, 2882
Bsp1286I GDGCHC 2 cut(s) 1351, 2910
Bsp1407I TGTACA 2 cut(s) 25, 2544
BspACI CCGC 7 cut(s) 816, 1013, 1382, 1772, 2094, 2610, 3018
BspANI GGCC 5 cut(s) 478, 499, 756, 2123, 2171
BspLI GGNNCC 7 cut(s) 681, 762, 846, 1494, 2409, 2478, 2781
BspMAI CTGCAG 1 cut(s) 76
BspOI GCTAGC 1 cut(s) 2490
BspQI GCTCTTC 2 cut(s) 2190, 2558
BspT107I GGYRCC 3 cut(s) 1492, 2407, 2476
BsrBI CCGCTC 2 cut(s) 818, 3020
BsrDI GCAATG 6 cut(s) 319, 2124, 2348, 2497, 2605, 2788
BsrFI RCCGGY 2 cut(s) 2344, 2353
BsrGI TGTACA 2 cut(s) 25, 2544
BsrI ACTGG 3 cut(s) 1175, 1276, 2458
BssAI RCCGGY 2 cut(s) 2344, 2353
BssECI CCNNGG 2 cut(s) 624, 1925
BssNAI GTATAC 1 cut(s) 596
BssNI GRCGYC 1 cut(s) 2675
BssSI CACGAG 1 cut(s) 1105
Bst1107I GTATAC 1 cut(s) 596
Bst2BI CACGAG 1 cut(s) 1105
Bst2UI CCWGG 6 cut(s) 187, 625, 663, 744, 1926, 2246
Bst4CI ACNGT 8 cut(s) 926, 1486, 1579, 2392, 2404, 2467, 2807, 2949
Bst6I CTCTTC 7 cut(s) 741, 1826, 2139, 2190, 2558, 2709, 2928
BstACI GRCGYC 1 cut(s) 2675
BstAPI GCANNNNNTGC 2 cut(s) 180, 1901
BstAUI TGTACA 2 cut(s) 25, 2544
BstBAI YACGTR 1 cut(s) 1778
BstC8I GCNNGC 6 cut(s) 391, 1179, 1435, 2202, 2346, 2488
BstF5I GGATG 6 cut(s) 613, 658, 1194, 1457, 1978, 2839
BstH2I RGCGCY 2 cut(s) 2766, 2882
BstHHI GCGC 3 cut(s) 2765, 2881, 2999
BstMAI GTCTC 4 cut(s) 858, 2504, 2666, 2879
BstMCI CGRYCG 1 cut(s) 2017
BstNI CCWGG 6 cut(s) 187, 625, 663, 744, 1926, 2246
BstNSI RCATGY 7 cut(s) 32, 81, 200, 382, 776, 2620, 2914
BstSCI CCNGG 7 cut(s) 185, 623, 661, 742, 756, 1924, 2244
BstSFI CTRYAG 4 cut(s) 72, 1482, 2138, 2945
BstV2I GAAGAC 4 cut(s) 626, 927, 1812, 2817
BstX2I RGATCY 4 cut(s) 238, 679, 760, 844
BstYI RGATCY 4 cut(s) 238, 679, 760, 844
BstZ17I GTATAC 1 cut(s) 596
BsuI GTATCC 2 cut(s) 773, 857
BsuRI GGCC 5 cut(s) 478, 499, 756, 2123, 2171
BtsCI GGATG 6 cut(s) 613, 658, 1194, 1457, 1978, 2839
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 5 cut(s) 214, 867, 1491, 1993, 2397
Cac8I GCNNGC 6 cut(s) 391, 1179, 1435, 2202, 2346, 2488
CaiI CAGNNNCTG 2 cut(s) 2471, 2974
CfoI GCGC 3 cut(s) 2765, 2881, 2999
Cfr10I RCCGGY 2 cut(s) 2344, 2353
Cfr13I GGNCC 3 cut(s) 432, 2170, 2780
CseI GACGC 2 cut(s) 708, 2683
EaeI YGGCCR 2 cut(s) 754, 2121
Eam1104I CTCTTC 7 cut(s) 741, 1826, 2139, 2190, 2558, 2709, 2928
EarI CTCTTC 7 cut(s) 741, 1826, 2139, 2190, 2558, 2709, 2928
EciI GGCGGA 2 cut(s) 1371, 2599
Eco147I AGGCCT 1 cut(s) 499
Eco32I GATATC 2 cut(s) 1234, 2308
Eco47I GGWCC 2 cut(s) 432, 2780
Eco47III AGCGCT 1 cut(s) 2764
Eco57I CTGAAG 3 cut(s) 975, 2159, 2433
Eco72I CACGTG 1 cut(s) 1778
Eco88I CYCGRG 2 cut(s) 522, 2882
EcoO109I RGGNCCY 1 cut(s) 2170
EcoRI GAATTC 1 cut(s) 1189
EcoRII CCWGG 6 cut(s) 185, 623, 661, 742, 1924, 2244
EcoRV GATATC 2 cut(s) 1234, 2308
EcoT22I ATGCAT 1 cut(s) 1207
Esp3I CGTCTC 1 cut(s) 2666
FalI AAGNNNNNCTT 2 cut(s) 1139, 1171
FaqI GGGAC 2 cut(s) 1288, 2793
FauNDI CATATG 2 cut(s) 343, 2163
FbaI TGATCA 2 cut(s) 2127, 2890
FblI GTMKAC 1 cut(s) 595
FokI GGATG 6 cut(s) 620, 645, 1181, 1444, 1985, 2846
FspBI CTAG 6 cut(s) 510, 1068, 1226, 2288, 2487, 2637
GlaI GCGC 3 cut(s) 2764, 2880, 2998
GsaI CCCAGC 2 cut(s) 679, 844
GsuI CTGGAG 1 cut(s) 169
HaeII RGCGCY 2 cut(s) 2766, 2882
HaeIII GGCC 5 cut(s) 478, 499, 756, 2123, 2171
HapII CCGG 5 cut(s) 420, 757, 1613, 2345, 2354
HgaI GACGC 2 cut(s) 708, 2683
HhaI GCGC 3 cut(s) 2765, 2881, 2999
Hin1I GRCGYC 1 cut(s) 2675
Hin6I GCGC 3 cut(s) 2763, 2879, 2997
HinP1I GCGC 3 cut(s) 2763, 2879, 2997
HindIII AAGCTT 2 cut(s) 969, 2579
HpaII CCGG 5 cut(s) 420, 757, 1613, 2345, 2354
HphI GGTGA 6 cut(s) 1172, 1613, 1706, 1799, 1892, 2012
Hpy166II GTNNAC 2 cut(s) 58, 596
Hpy8I GTNNAC 2 cut(s) 58, 596
Hpy99I CGWCG 1 cut(s) 825
HpyAV CCTTC 8 cut(s) 958, 1442, 1483, 1970, 1991, 2183, 2368, 2933
HpyCH4III ACNGT 8 cut(s) 926, 1486, 1579, 2392, 2404, 2467, 2807, 2949
HpyCH4IV ACGT 3 cut(s) 574, 1047, 1777
HpySE526I ACGT 3 cut(s) 574, 1047, 1777
Hsp92I GRCGYC 1 cut(s) 2675
HspAI GCGC 3 cut(s) 2763, 2879, 2997
KpnI GGTACC 3 cut(s) 1496, 2411, 2480
KroI GCCGGC 1 cut(s) 2344
KroNI GCCGGC 1 cut(s) 2346
Ksp22I TGATCA 2 cut(s) 2127, 2890
LguI GCTCTTC 2 cut(s) 2190, 2558
LmnI GCTCC 6 cut(s) 188, 955, 1346, 2773, 2971, 3025
LweI GCATC 9 cut(s) 183, 342, 1282, 1612, 1705, 1798, 1891, 2209, 2721
MaeI CTAG 6 cut(s) 510, 1068, 1226, 2288, 2487, 2637
MaeII ACGT 3 cut(s) 574, 1047, 1777
MaeIII GTNAC 6 cut(s) 646, 1048, 1615, 1787, 2456, 2467
MbiI CCGCTC 2 cut(s) 818, 3020
MflI RGATCY 4 cut(s) 238, 679, 760, 844
MhlI GDGCHC 2 cut(s) 1351, 2910
MlsI TGGCCA 1 cut(s) 2123
MluNI TGGCCA 1 cut(s) 2123
MlyI GAGTC 4 cut(s) 722, 1192, 1436, 1523
MmeI TCCRAC 2 cut(s) 378, 2853
Mox20I TGGCCA 1 cut(s) 2123
Mph1103I ATGCAT 1 cut(s) 1207
MroNI GCCGGC 1 cut(s) 2344
MscI TGGCCA 1 cut(s) 2123
MslI CAYNNNNRTG 3 cut(s) 82, 1407, 2649
Msp20I TGGCCA 1 cut(s) 2123
MspA1I CMGCKG 2 cut(s) 1772, 2096
MspI CCGG 5 cut(s) 420, 757, 1613, 2345, 2354
MspR9I CCNGG 7 cut(s) 187, 625, 663, 744, 758, 1926, 2246
Mva1269I GAATGC 3 cut(s) 1205, 2095, 2632
MvaI CCWGG 6 cut(s) 187, 625, 663, 744, 1926, 2246
NaeI GCCGGC 1 cut(s) 2346
NciI CCSGG 1 cut(s) 758
NdeI CATATG 2 cut(s) 343, 2163
NgoMIV GCCGGC 1 cut(s) 2344
NheI GCTAGC 1 cut(s) 2486
NlaIV GGNNCC 7 cut(s) 681, 762, 846, 1494, 2409, 2478, 2781
NmuCI GTSAC 1 cut(s) 1787
NsiI ATGCAT 1 cut(s) 1207
NspI RCATGY 7 cut(s) 32, 81, 200, 382, 776, 2620, 2914
PaeR7I CTCGAG 2 cut(s) 522, 2882
PceI AGGCCT 1 cut(s) 499
PciI ACATGT 4 cut(s) 28, 196, 378, 772
PciSI GCTCTTC 2 cut(s) 2190, 2558
PcsI WCGNNNNNNNCGW 3 cut(s) 1053, 1797, 2023
PctI GAATGC 3 cut(s) 1205, 2095, 2632
PdiI GCCGGC 1 cut(s) 2346
PfoI TCCNGGA 1 cut(s) 661
Ple19I CGATCG 1 cut(s) 2017
PleI GAGTC 4 cut(s) 722, 1191, 1435, 1523
PmaCI CACGTG 1 cut(s) 1778
PmlI CACGTG 1 cut(s) 1778
PpsI GAGTC 4 cut(s) 722, 1191, 1435, 1523
Ppu21I YACGTR 1 cut(s) 1778
PscI ACATGT 4 cut(s) 28, 196, 378, 772
PshBI ATTAAT 2 cut(s) 798, 1407
Psp1406I AACGTT 1 cut(s) 1047
Psp6I CCWGG 6 cut(s) 185, 623, 661, 742, 1924, 2244
PspCI CACGTG 1 cut(s) 1778
PspFI CCCAGC 2 cut(s) 675, 840
PspGI CCWGG 6 cut(s) 185, 623, 661, 742, 1924, 2244
PspN4I GGNNCC 7 cut(s) 681, 762, 846, 1494, 2409, 2478, 2781
PspPI GGNCC 3 cut(s) 432, 2170, 2780
PspXI VCTCGAGB 1 cut(s) 522
PstI CTGCAG 1 cut(s) 76
PstNI CAGNNNCTG 2 cut(s) 2471, 2974
PsuI RGATCY 4 cut(s) 238, 679, 760, 844
PvuI CGATCG 1 cut(s) 2017
RseI CAYNNNNRTG 3 cut(s) 82, 1407, 2649
SapI GCTCTTC 2 cut(s) 2190, 2558
Sau96I GGNCC 3 cut(s) 432, 2170, 2780
ScaI AGTACT 1 cut(s) 2401
SchI GAGTC 4 cut(s) 722, 1192, 1436, 1523
ScrFI CCNGG 7 cut(s) 187, 625, 663, 744, 758, 1926, 2246
SduI GDGCHC 2 cut(s) 1351, 2910
SfaNI GCATC 9 cut(s) 183, 342, 1282, 1612, 1705, 1798, 1891, 2209, 2721
SfcI CTRYAG 4 cut(s) 72, 1482, 2138, 2945
Sfr274I CTCGAG 2 cut(s) 522, 2882
SinI GGWCC 2 cut(s) 432, 2780
SlaI CTCGAG 2 cut(s) 522, 2882
SmiMI CAYNNNNRTG 3 cut(s) 82, 1407, 2649
SmlI CTYRAG 3 cut(s) 522, 2882, 2903
SmoI CTYRAG 3 cut(s) 522, 2882, 2903
SseBI AGGCCT 1 cut(s) 499
SsiI CCGC 7 cut(s) 816, 1013, 1382, 1772, 2094, 2610, 3018
SspI AATATT 2 cut(s) 152, 562
SspMI CTAG 6 cut(s) 510, 1068, 1226, 2288, 2487, 2637
StuI AGGCCT 1 cut(s) 499
StyD4I CCNGG 7 cut(s) 185, 623, 661, 742, 756, 1924, 2244
TaaI ACNGT 8 cut(s) 926, 1486, 1579, 2392, 2404, 2467, 2807, 2949
TaiI ACGT 3 cut(s) 577, 1050, 1780
TaqI TCGA 9 cut(s) 111, 523, 1133, 2013, 2226, 2304, 2321, 2445, 2883
TatI WGTACW 4 cut(s) 25, 1667, 2399, 2544
TauI GCSGC 2 cut(s) 1775, 2096
TscAI CASTG 5 cut(s) 214, 874, 1491, 1993, 2397
TseFI GTSAC 1 cut(s) 1787
Tsp45I GTSAC 1 cut(s) 1787
TspGWI ACGGA 3 cut(s) 1541, 2772, 3003
TspRI CASTG 5 cut(s) 214, 874, 1491, 1993, 2397
VpaK11BI GGWCC 2 cut(s) 432, 2780
VspI ATTAAT 2 cut(s) 798, 1407
XbaI TCTAGA 1 cut(s) 2636
XceI RCATGY 7 cut(s) 32, 81, 200, 382, 776, 2620, 2914
XcmI CCANNNNNNNNNTGG 1 cut(s) 2242
XhoI CTCGAG 2 cut(s) 522, 2882
XmiI GTMKAC 1 cut(s) 595
XspI CTAG 6 cut(s) 510, 1068, 1226, 2288, 2487, 2637
ZrmI AGTACT 1 cut(s) 2401
Zsp2I ATGCAT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.