Rroxscaffold_7G00179540

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003313
Physical Location & Seq
Forward (+)
19073618 .. 19078145
4528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00179540.1

Sequence Viewer

Length: 2235 bp
ATGGCAGCAAAGGGGAAGCCATCAGTAGAAGTGCAGAGGAACTTTGAGAGGTTTATAGCCATTCTAACATCTGCAGCATGTGAATGGTTTTTGATATTTCTTCTGTTTGTCGAAGCACTGTTATCCTATCTGCTGACTAAGTTTGCGGTCCATTGCGACTTGCAAAAGCCCTGCATCTTGTGCTCAAGGTTTGAACATATTACCGGTAATGAAAGTTCTCAACTTTATGAGGATCTACTTTGCAGCAACCACATATTGAAGCTCACATCTTGGATAGCTAGTCAAATTTATGGTAAGGATGATGGCAATGGAATGCATGAAGATTATCACTTTCCTTTCACCACAAGGGACAACTCCAACCAAGAAATGCATATGTTTTCAGTTGGTAATTCGGTATCAGATGTTGAAAGTTATGGTTCTCAGAGGCTTGTGCCAAGCAGAACTTGTGTTCATAGTCCTGTTCACAGTTCTGTGGGTGTAGGGCAATGGTCTTGCAGCTGTAGTCTTTGGGGACATAGAGGAAAGTTTAAAGGACTAAAACTCAAATCATCTGGGACAAAACATGCCAAGCCTAATATTCCACTGCCACGTTTGCCAAACAGTAGCCGTTCAAACCGAAGCCGGGATAGTTCAAAGAAGATCAGGGATAAGTCTCCGGCGTCAGTCACTAGTCGTCGTTCTGCAAAAGTAGGAGTTGATCCCTTGTTTCATGTAAGATACAGTGAATTGAAGTTCTTCTCTGATACTGACTCAGAGTTTCTGTTTTCTGATGGGGATGATGGTAGCGGTTTAACCAGTGTAAATCATGAGCCTAAGATGGAAGGTTTAGTACGTGCTTCACGATATCGGCCTAAAGCACCACCAAATGGTTTGAGGTCAGGAAGGCCTAGCAAGCGTCCCCCCAAACCCAAGCGGTCACTTTCAGAACCATGGCCTGTTTTTAGGAAAGCCTATGATGTAAAATCCTTGGGTTCTTATAACGAATTTGAGACTGGCCTCAGTGAGCTTAACCGTCAACAATCCAATAGGAAGTCCAACCCTTCTGCATTACCTGAGCTCATTTCACTAGATGACATCCTTTCGTCACCCAATGTTCTAGAAAAGGCAGATATTAATGGGACAGGGGACTTTGGATATGCATCCATCAATAAGAATGTTGAACTTCTGAAGTCTGTATGTGCAACAGACGGAGCACCTGTTAAAAACGATCCAGCTCTTATAATACCAAACCATAGTAAGGTAGATGCTGTGTCTGAGTCAGAGGTAAGTGTTAAGGAAAGAGATTCATCTGGTACTACTACAGAAGCTCCTCTTATGAAACCAAACCCCAGTAATGTAAATGCTGTGTCCAAGTCACAGGGAAGTGTTCGAGAAAGAGATTCATCTGGTATTACTACTGAAGCTTTTATAAAACCAGACCATAATAAGGTAAATGCTGTGTCACAGTCAGAGGGAAATGTTAAGGAAAGAGCTTCATCCAGTATTACTACAGAAGCTCTTATGCAACCAGAACATGGTAAGGTACATCCTCTGTCTCAGTCAGACAGAAATGTTGAGGAAAGAGATTCTTCTGGTGTTTCTAAAGAACGGCCAGCAGTGAAGGAACCCGATAGAGTTGATGAGGAAATGGAAGTGCTAGCTTCACAGGACTCTTCTGCTCGGGAGACTTACTTGTCAGCAAAGAATACAAGTTCAACAGTAGATGGTTGTGTGGGTGAAAAACAGACAACTGATGCCTCCAGCTCTAATGCAAAGCCGATGCTTCACACGTCAGCATCAGTAGAATCTGGTCTTGAATCGCTGGATGGAGGCTATGTCAGTGAAATTGAAGGTGAAAGTATAGTTGATCAGTTAAAACGACAGATTGATTATGACAGGAAATGCATAAAGGAGTTGTATAAGGAACTGGAAGAAGAAAGGAGTGCCGCTGCAATTGCTGCAAATCAGGCAATGGCCATGATCACAAAGCTCCAAGAAGAGAAAGCTGCATTCCATATGGAGGCCCTTCACTGCTTAAGAATGATGGAAGAGCAGGCTGAGTTTGACGTAGAGGCACTGGAGAAAGCCAATGATCTCCTGGCTGAGAAGGAGAAAGAGATACAGGACTTGGAAGCAGAGCTAGAATTTTCTAAGTTAGACTTCCAAGATGAATCGATAATGGGGAATCCACTTGACAAAAGTTGTGATTTGATGGGGGGATTGACCTGGTGGAGAATACTGTTATGCCCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

744

Amino Acids

82.29

Weight (kDa)

5.69

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 617 - 707 1.9e-28 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 978, 1220, 1409
AasI GACNNNNNNGTC 1 cut(s) 1672
AccB7I CCANNNNNTGG 2 cut(s) 866, 1514
AciI CCGC 4 cut(s) 146, 786, 913, 1926
AclWI GGATC 3 cut(s) 240, 692, 1202
AcoI YGGCCR 2 cut(s) 1589, 1953
AcsI RAATTY 3 cut(s) 285, 983, 2123
AcuI CTGAAG 2 cut(s) 1187, 1419
AcyI GRCGYC 1 cut(s) 659
AfaI GTAC 3 cut(s) 831, 1294, 1524
AfiI CCNNNNNNNGG 6 cut(s) 622, 866, 1237, 1426, 1514, 1999
AflII CTTAAG 1 cut(s) 2014
AflIII ACRYGT 1 cut(s) 1767
AgeI ACCGGT 1 cut(s) 203
AhlI ACTAGT 1 cut(s) 668
AjiI CACGTC 1 cut(s) 1770
AjnI CCWGG 2 cut(s) 2076, 2204
Alw21I GWGCWC 3 cut(s) 185, 1059, 1195
Alw26I GTCTC 4 cut(s) 657, 983, 1539, 1658
AlwI GGATC 3 cut(s) 240, 692, 1202
Ama87I CYCGRG 1 cut(s) 1659
AoxI GGCC 7 cut(s) 848, 884, 932, 994, 1589, 1953, 2001
ApeKI GCWGC 7 cut(s) 5, 74, 243, 495, 1928, 1937, 1985
ApoI RAATTY 3 cut(s) 285, 983, 2123
AseI ATTAAT 1 cut(s) 1113
AsiGI ACCGGT 1 cut(s) 203
Asp700I GAANNNNTTC 1 cut(s) 734
AspS9I GGNCC 2 cut(s) 148, 2002
AsuC2I CCSGG 1 cut(s) 623
AsuHPI GGTGA 4 cut(s) 331, 1077, 1727, 1844
AsuNHI GCTAGC 1 cut(s) 1636
AvaI CYCGRG 1 cut(s) 1659
AvaII GGWCC 1 cut(s) 148
BalI TGGCCA 1 cut(s) 1955
BanII GRGCYC 1 cut(s) 1059
BarI GAAGNNNNNNTAC 2 cut(s) 813, 845
Bbv12I GWGCWC 3 cut(s) 185, 1059, 1195
BbvI GCAGC 7 cut(s) 17, 86, 255, 507, 1915, 1924, 1972
BceAI ACGGC 2 cut(s) 591, 1604
BciT130I CCWGG 2 cut(s) 2078, 2206
BclI TGATCA 2 cut(s) 1846, 1959
BcnI CCSGG 1 cut(s) 623
BcoDI GTCTC 4 cut(s) 657, 983, 1539, 1658
BcuI ACTAGT 1 cut(s) 668
BfaI CTAG 7 cut(s) 279, 669, 888, 1067, 1097, 1637, 2120
BfmI CTRYAG 4 cut(s) 72, 499, 1299, 1488
BfrI CTTAAG 1 cut(s) 2014
BisI GCNGC 8 cut(s) 6, 75, 244, 496, 1926, 1929, 1938, 1986
BlsI GCNGC 8 cut(s) 7, 76, 245, 497, 1927, 1930, 1939, 1987
Bme1390I CCNGG 3 cut(s) 623, 2078, 2206
Bme18I GGWCC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 1659
BmgBI CACGTC 1 cut(s) 1770
BmgT120I GGNCC 2 cut(s) 148, 2002
BmiI GGNNCC 1 cut(s) 1605
BmrFI CCNGG 3 cut(s) 623, 2078, 2206
BmrI ACTGGG 1 cut(s) 1323
BmsI GCATC 6 cut(s) 183, 1148, 1234, 1723, 1749, 1784
BmtI GCTAGC 1 cut(s) 1640
BmuI ACTGGG 1 cut(s) 1323
BpmI CTGGAG 2 cut(s) 1723, 2078
Bpu10I CCTNAGC 1 cut(s) 1053
BpuEI CTTGAG 1 cut(s) 169
BpuMI CCSGG 1 cut(s) 623
Bsa29I ATCGAT 1 cut(s) 2153
BsaAI YACGTR 1 cut(s) 833
BsaBI GATNNNNATC 1 cut(s) 1138
BsaHI GRCGYC 1 cut(s) 659
BsaJI CCNNGG 2 cut(s) 929, 966
BsaWI WCCGGW 1 cut(s) 203
BsaXI ACNNNNNCTCC 6 cut(s) 1182, 1212, 1291, 1321, 1800, 1830
Bsc4I CCNNNNNNNGG 6 cut(s) 622, 866, 1237, 1426, 1514, 1999
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 6 cut(s) 795, 997, 1329, 1479, 1911, 2061
Bse3DI GCAATG 4 cut(s) 151, 313, 491, 1956
Bse8I GATNNNNATC 1 cut(s) 1138
BseBI CCWGG 2 cut(s) 2078, 2206
BseCI ATCGAT 1 cut(s) 2153
BseDI CCNNGG 2 cut(s) 929, 966
BseGI GGATG 7 cut(s) 304, 781, 1074, 1139, 1475, 1525, 1810
BseJI GATNNNNATC 1 cut(s) 1138
BseLI CCNNNNNNNGG 6 cut(s) 622, 866, 1237, 1426, 1514, 1999
BseMI GCAATG 4 cut(s) 151, 313, 491, 1956
BseMII CTCAG 8 cut(s) 434, 765, 1012, 1044, 1245, 1550, 2028, 2073
BseNI ACTGG 6 cut(s) 795, 997, 1329, 1479, 1911, 2061
BseRI GAGGAG 1 cut(s) 1299
BseXI GCAGC 7 cut(s) 17, 86, 255, 507, 1915, 1924, 1972
BsgI GTGCAG 1 cut(s) 53
BshFI GGCC 7 cut(s) 850, 886, 934, 996, 1591, 1955, 2003
BshTI ACCGGT 1 cut(s) 203
BshVI ATCGAT 1 cut(s) 2153
BsiHKAI GWGCWC 3 cut(s) 185, 1059, 1195
BsiHKCI CYCGRG 1 cut(s) 1659
BsiSI CCGG 3 cut(s) 204, 622, 656
BslFI GGGAC 6 cut(s) 362, 525, 568, 882, 1132, 1139
BslI CCNNNNNNNGG 6 cut(s) 622, 866, 1237, 1426, 1514, 1999
BsmAI GTCTC 4 cut(s) 657, 983, 1539, 1658
BsmFI GGGAC 6 cut(s) 362, 525, 568, 882, 1132, 1139
BsmI GAATGC 2 cut(s) 318, 1988
BsnI GGCC 7 cut(s) 850, 886, 934, 996, 1591, 1955, 2003
BsoBI CYCGRG 1 cut(s) 1659
Bsp1286I GDGCHC 3 cut(s) 185, 1059, 1195
Bsp143I GATC 7 cut(s) 232, 639, 697, 1207, 1846, 1959, 2071
Bsp19I CCATGG 1 cut(s) 929
BspACI CCGC 4 cut(s) 146, 786, 913, 1926
BspANI GGCC 7 cut(s) 850, 886, 934, 996, 1591, 1955, 2003
BspCNI CTCAG 8 cut(s) 433, 764, 1011, 1045, 1246, 1549, 2029, 2074
BspDI ATCGAT 1 cut(s) 2153
BspHI TCATGA 1 cut(s) 805
BspLI GGNNCC 1 cut(s) 1605
BspMAI CTGCAG 1 cut(s) 76
BspOI GCTAGC 1 cut(s) 1640
BspPI GGATC 3 cut(s) 240, 692, 1202
BspQI GCTCTTC 1 cut(s) 2022
BspTI CTTAAG 1 cut(s) 2014
BsrDI GCAATG 4 cut(s) 151, 313, 491, 1956
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 6 cut(s) 795, 997, 1329, 1479, 1911, 2061
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 929, 966
BssMI GATC 7 cut(s) 232, 639, 697, 1207, 1846, 1959, 2071
BssNI GRCGYC 1 cut(s) 659
BssT1I CCWWGG 2 cut(s) 929, 966
Bst2UI CCWGG 2 cut(s) 2078, 2206
Bst4CI ACNGT 8 cut(s) 120, 467, 602, 722, 1013, 1446, 1699, 2220
Bst6I CTCTTC 3 cut(s) 1657, 1971, 2022
BstACI GRCGYC 1 cut(s) 659
BstAFI CTTAAG 1 cut(s) 2014
BstAPI GCANNNNNTGC 2 cut(s) 180, 1937
BstBAI YACGTR 1 cut(s) 833
BstC8I GCNNGC 4 cut(s) 893, 1593, 1638, 2034
BstDSI CCRYGG 1 cut(s) 929
BstF5I GGATG 7 cut(s) 304, 781, 1074, 1139, 1475, 1525, 1810
BstKTI GATC 7 cut(s) 235, 642, 700, 1210, 1849, 1962, 2074
BstMAI GTCTC 4 cut(s) 657, 983, 1539, 1658
BstMBI GATC 7 cut(s) 232, 639, 697, 1207, 1846, 1959, 2071
BstMWI GCNNNNNNNGC 6 cut(s) 180, 592, 892, 1934, 1937, 1946
BstNI CCWGG 2 cut(s) 2078, 2206
BstNSI RCATGY 2 cut(s) 81, 566
BstSCI CCNGG 3 cut(s) 621, 2076, 2204
BstSFI CTRYAG 4 cut(s) 72, 499, 1299, 1488
BstV1I GCAGC 7 cut(s) 17, 86, 255, 507, 1915, 1924, 1972
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
Bsu15I ATCGAT 1 cut(s) 2153
BsuRI GGCC 7 cut(s) 850, 886, 934, 996, 1591, 1955, 2003
BsuTUI ATCGAT 1 cut(s) 2153
BtgI CCRYGG 1 cut(s) 929
BtrI CACGTC 1 cut(s) 1770
BtsCI GGATG 7 cut(s) 304, 781, 1074, 1139, 1475, 1525, 1810
BtsI GCAGTG 3 cut(s) 581, 1602, 2008
BtsIMutI CAGTG 9 cut(s) 116, 581, 727, 802, 1006, 1602, 1825, 2008, 2054
Cac8I GCNNGC 4 cut(s) 893, 1593, 1638, 2034
CciI TCATGA 1 cut(s) 805
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 2 cut(s) 148, 2002
ClaI ATCGAT 1 cut(s) 2153
CseI GACGC 2 cut(s) 648, 884
CsiI ACCWGGT 1 cut(s) 2204
Csp6I GTAC 3 cut(s) 830, 1293, 1523
CspAI ACCGGT 1 cut(s) 203
CviAII CATG 8 cut(s) 78, 317, 563, 710, 806, 930, 1514, 1957
CviQI GTAC 3 cut(s) 830, 1293, 1523
DpnI GATC 7 cut(s) 234, 641, 699, 1209, 1848, 1961, 2073
DpnII GATC 7 cut(s) 232, 639, 697, 1207, 1846, 1959, 2071
DraI TTTAAA 1 cut(s) 529
DrdI GACNNNNNNGTC 1 cut(s) 1672
DseDI GACNNNNNNGTC 1 cut(s) 1672
EaeI YGGCCR 2 cut(s) 1589, 1953
Eam1104I CTCTTC 3 cut(s) 1657, 1971, 2022
EarI CTCTTC 3 cut(s) 1657, 1971, 2022
Ecl136II GAGCTC 1 cut(s) 1057
Eco130I CCWWGG 2 cut(s) 929, 966
Eco147I AGGCCT 1 cut(s) 886
Eco24I GRGCYC 1 cut(s) 1059
Eco32I GATATC 1 cut(s) 845
Eco47I GGWCC 1 cut(s) 148
Eco53kI GAGCTC 1 cut(s) 1057
Eco57I CTGAAG 2 cut(s) 1187, 1419
Eco88I CYCGRG 1 cut(s) 1659
EcoICRI GAGCTC 1 cut(s) 1057
EcoO109I RGGNCCY 1 cut(s) 2002
EcoRII CCWGG 2 cut(s) 2076, 2204
EcoRV GATATC 1 cut(s) 845
EcoT14I CCWWGG 2 cut(s) 929, 966
EcoT22I ATGCAT 4 cut(s) 318, 372, 1141, 1886
EcoT38I GRGCYC 1 cut(s) 1059
ErhI CCWWGG 2 cut(s) 929, 966
FaeI CATG 8 cut(s) 81, 320, 566, 713, 809, 933, 1517, 1960
FalI AAGNNNNNCTT 8 cut(s) 427, 459, 1296, 1328, 1552, 1584, 2123, 2155
FaqI GGGAC 6 cut(s) 362, 525, 568, 882, 1132, 1139
FatI CATG 8 cut(s) 77, 316, 562, 709, 805, 929, 1513, 1956
FauNDI CATATG 2 cut(s) 372, 1995
FbaI TGATCA 2 cut(s) 1846, 1959
Fnu4HI GCNGC 8 cut(s) 6, 75, 244, 496, 1926, 1929, 1938, 1986
FokI GGATG 7 cut(s) 311, 788, 1061, 1126, 1462, 1512, 1817
FriOI GRGCYC 1 cut(s) 1059
Fsp4HI GCNGC 8 cut(s) 6, 75, 244, 496, 1926, 1929, 1938, 1986
FspBI CTAG 7 cut(s) 279, 669, 888, 1067, 1097, 1637, 2120
GluI GCNGC 8 cut(s) 6, 75, 244, 496, 1926, 1929, 1938, 1986
GsuI CTGGAG 2 cut(s) 1723, 2078
HaeIII GGCC 7 cut(s) 850, 886, 934, 996, 1591, 1955, 2003
HapII CCGG 3 cut(s) 204, 622, 656
HgaI GACGC 2 cut(s) 648, 884
Hin1I GRCGYC 1 cut(s) 659
Hin1II CATG 8 cut(s) 81, 320, 566, 713, 809, 933, 1517, 1960
HincII GTYRAC 1 cut(s) 1016
HindII GTYRAC 1 cut(s) 1016
HindIII AAGCTT 1 cut(s) 1401
HpaII CCGG 3 cut(s) 204, 622, 656
HphI GGTGA 4 cut(s) 331, 1077, 1727, 1844
Hpy166II GTNNAC 2 cut(s) 463, 1016
Hpy188III TCNNGA 7 cut(s) 806, 840, 879, 1097, 1370, 1661, 1793
Hpy8I GTNNAC 2 cut(s) 463, 1016
Hpy99I CGWCG 1 cut(s) 678
HpyAV CCTTC 7 cut(s) 815, 876, 1050, 1594, 1823, 2015, 2080
HpyCH4III ACNGT 8 cut(s) 120, 467, 602, 722, 1013, 1446, 1699, 2220
HpyCH4IV ACGT 4 cut(s) 589, 832, 1769, 2046
HpyF10VI GCNNNNNNNGC 6 cut(s) 180, 592, 892, 1934, 1937, 1946
HpySE526I ACGT 4 cut(s) 589, 832, 1769, 2046
Hsp92I GRCGYC 1 cut(s) 659
Hsp92II CATG 8 cut(s) 81, 320, 566, 713, 809, 933, 1517, 1960
Ksp22I TGATCA 2 cut(s) 1846, 1959
Kzo9I GATC 7 cut(s) 232, 639, 697, 1207, 1846, 1959, 2071
LguI GCTCTTC 1 cut(s) 2022
LmnI GCTCC 3 cut(s) 1190, 1312, 1974
Lsp1109I GCAGC 7 cut(s) 17, 86, 255, 507, 1915, 1924, 1972
LweI GCATC 6 cut(s) 183, 1148, 1234, 1723, 1749, 1784
MabI ACCWGGT 1 cut(s) 2204
MaeI CTAG 7 cut(s) 279, 669, 888, 1067, 1097, 1637, 2120
MaeII ACGT 4 cut(s) 589, 832, 1769, 2046
MaeIII GTNAC 5 cut(s) 664, 915, 1083, 1353, 1440
MalI GATC 7 cut(s) 234, 641, 699, 1209, 1848, 1961, 2073
MboI GATC 7 cut(s) 232, 639, 697, 1207, 1846, 1959, 2071
MfeI CAATTG 1 cut(s) 1932
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 3 cut(s) 185, 1059, 1195
MlsI TGGCCA 1 cut(s) 1955
MluCI AATT 7 cut(s) 285, 388, 725, 983, 1824, 1932, 2123
MluNI TGGCCA 1 cut(s) 1955
MlyI GAGTC 3 cut(s) 743, 1265, 1643
MmeI TCCRAC 2 cut(s) 381, 1059
Mox20I TGGCCA 1 cut(s) 1955
Mph1103I ATGCAT 4 cut(s) 318, 372, 1141, 1886
MroXI GAANNNNTTC 1 cut(s) 734
MscI TGGCCA 1 cut(s) 1955
MseI TTAA 9 cut(s) 528, 791, 1008, 1113, 1200, 1272, 1461, 1853, 2015
MslI CAYNNNNRTG 1 cut(s) 82
Msp20I TGGCCA 1 cut(s) 1955
MspA1I CMGCKG 2 cut(s) 498, 1928
MspCI CTTAAG 1 cut(s) 2014
MspI CCGG 3 cut(s) 204, 622, 656
MspR9I CCNGG 3 cut(s) 623, 2078, 2206
MunI CAATTG 1 cut(s) 1932
Mva1269I GAATGC 2 cut(s) 318, 1988
MvaI CCWGG 2 cut(s) 2078, 2206
MwoI GCNNNNNNNGC 6 cut(s) 180, 592, 892, 1934, 1937, 1946
NciI CCSGG 1 cut(s) 623
NcoI CCATGG 1 cut(s) 929
NdeI CATATG 2 cut(s) 372, 1995
NdeII GATC 7 cut(s) 232, 639, 697, 1207, 1846, 1959, 2071
NheI GCTAGC 1 cut(s) 1636
NlaIII CATG 8 cut(s) 81, 320, 566, 713, 809, 933, 1517, 1960
NlaIV GGNNCC 1 cut(s) 1605
NmuCI GTSAC 5 cut(s) 664, 915, 1083, 1353, 1440
NsiI ATGCAT 4 cut(s) 318, 372, 1141, 1886
NspI RCATGY 2 cut(s) 81, 566
PagI TCATGA 1 cut(s) 805
PceI AGGCCT 1 cut(s) 886
PciSI GCTCTTC 1 cut(s) 2022
PcsI WCGNNNNNNNCGW 1 cut(s) 838
PctI GAATGC 2 cut(s) 318, 1988
PdmI GAANNNNTTC 1 cut(s) 734
PfeI GAWTC 7 cut(s) 1283, 1379, 1565, 1784, 1796, 2150, 2164
PflMI CCANNNNNTGG 2 cut(s) 866, 1514
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 8 cut(s) 7, 76, 245, 497, 1927, 1930, 1939, 1987
PleI GAGTC 3 cut(s) 743, 1264, 1643
PpsI GAGTC 3 cut(s) 743, 1264, 1643
Ppu21I YACGTR 1 cut(s) 833
PshBI ATTAAT 1 cut(s) 1113
PsiI TTATAA 3 cut(s) 978, 1220, 1409
Psp124BI GAGCTC 1 cut(s) 1059
Psp6I CCWGG 2 cut(s) 2076, 2204
PspGI CCWGG 2 cut(s) 2076, 2204
PspN4I GGNNCC 1 cut(s) 1605
PspPI GGNCC 2 cut(s) 148, 2002
PsrI GAACNNNNNNTAC 2 cut(s) 199, 231
PstI CTGCAG 1 cut(s) 76
PsuI RGATCY 1 cut(s) 232
PvuII CAGCTG 1 cut(s) 498
RsaI GTAC 3 cut(s) 831, 1294, 1524
RsaNI GTAC 3 cut(s) 830, 1293, 1523
RseI CAYNNNNRTG 1 cut(s) 82
SacI GAGCTC 1 cut(s) 1059
SapI GCTCTTC 1 cut(s) 2022
SaqAI TTAA 9 cut(s) 528, 791, 1008, 1113, 1200, 1272, 1461, 1853, 2015
SatI GCNGC 8 cut(s) 6, 75, 244, 496, 1926, 1929, 1938, 1986
Sau3AI GATC 7 cut(s) 232, 639, 697, 1207, 1846, 1959, 2071
Sau96I GGNCC 2 cut(s) 148, 2002
SchI GAGTC 3 cut(s) 743, 1265, 1643
ScrFI CCNGG 3 cut(s) 623, 2078, 2206
SduI GDGCHC 3 cut(s) 185, 1059, 1195
SexAI ACCWGGT 1 cut(s) 2204
SfaNI GCATC 6 cut(s) 183, 1148, 1234, 1723, 1749, 1784
SfcI CTRYAG 4 cut(s) 72, 499, 1299, 1488
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 82
SmlI CTYRAG 2 cut(s) 184, 2014
SmoI CTYRAG 2 cut(s) 184, 2014
SpeI ACTAGT 1 cut(s) 668
Sse9I AATT 7 cut(s) 285, 388, 725, 983, 1824, 1932, 2123
SseBI AGGCCT 1 cut(s) 886
SsiI CCGC 4 cut(s) 146, 786, 913, 1926
SspI AATATT 1 cut(s) 577
SspMI CTAG 7 cut(s) 279, 669, 888, 1067, 1097, 1637, 2120
SstI GAGCTC 1 cut(s) 1059
StuI AGGCCT 1 cut(s) 886
StyD4I CCNGG 3 cut(s) 621, 2076, 2204
StyI CCWWGG 2 cut(s) 929, 966
TaaI ACNGT 8 cut(s) 120, 467, 602, 722, 1013, 1446, 1699, 2220
TaiI ACGT 4 cut(s) 592, 835, 1772, 2049
TaqI TCGA 3 cut(s) 111, 1369, 2153
TasI AATT 7 cut(s) 285, 388, 725, 983, 1824, 1932, 2123
TauI GCSGC 1 cut(s) 1928
TfiI GAWTC 7 cut(s) 1283, 1379, 1565, 1784, 1796, 2150, 2164
Tru1I TTAA 9 cut(s) 528, 791, 1008, 1113, 1200, 1272, 1461, 1853, 2015
Tru9I TTAA 9 cut(s) 528, 791, 1008, 1113, 1200, 1272, 1461, 1853, 2015
TscAI CASTG 9 cut(s) 123, 588, 727, 802, 1006, 1602, 1825, 2015, 2061
TseFI GTSAC 5 cut(s) 664, 915, 1083, 1353, 1440
TseI GCWGC 7 cut(s) 5, 74, 243, 495, 1928, 1937, 1985
Tsp45I GTSAC 5 cut(s) 664, 915, 1083, 1353, 1440
TspDTI ATGAA 9 cut(s) 225, 333, 440, 698, 1275, 1331, 1371, 1464, 2163
TspGWI ACGGA 1 cut(s) 1203
TspRI CASTG 9 cut(s) 123, 588, 727, 802, 1006, 1602, 1825, 2015, 2061
Van91I CCANNNNNTGG 2 cut(s) 866, 1514
Vha464I CTTAAG 1 cut(s) 2014
VpaK11BI GGWCC 1 cut(s) 148
VspI ATTAAT 1 cut(s) 1113
XapI RAATTY 3 cut(s) 285, 983, 2123
XbaI TCTAGA 1 cut(s) 1096
XceI RCATGY 2 cut(s) 81, 566
XcmI CCANNNNNNNNNTGG 1 cut(s) 2074
XmnI GAANNNNTTC 1 cut(s) 734
XspI CTAG 7 cut(s) 279, 669, 888, 1067, 1097, 1637, 2120
Zsp2I ATGCAT 4 cut(s) 318, 372, 1141, 1886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.