Rh6DG311500

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003313
Physical Location & Seq
Reverse (-)
51315050 .. 51318173
3124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG311500.1

Sequence Viewer

Length: 2910 bp
ATGGCAGCAAAGGGGAAGTCATCAGTACAAGTGCAGAGGAACTTTAAGAGGTTTATAGCCATTCTAACATCTGCAGCATGTGAATGGTTTTTGATATTTCTTCTGTTTGTTGATGCACTGTTATCCTATCTGCTGACTAAGTTTGCGGTCTATTGCGACTTGCAAAAGCCCTGCATCTTGTGCTCAAGGTTTGAACATATTACTGGTAATGAAAGTTCTCAACTTTATGAGGATCTACTTTGCAGCAACCACATATCGAAGCTCACATCTTGGATAGCTAGTCAAATTTATGGTAAGGATGATGGCAATGGAATGCATGAAGATTATCTCTTTCCTTTCACCACAAGGGACAACTCCAACCGAGAAATGCATATGTTTTCAGTTGGTAATTCGGTATCAGATGTTGAAAGTTATGGTTCTCGGAGGAGTATGCCAAGCAGAAGTTGTGTTCACAGTCCTGTTCACAGTTCTGTGGGTGTAGGGCAATGGTCTTGTTGCTGTAGTCTTTGGGGACATAGAGGAAAGTTTAAAGGACTAAAACTCAAATCATCTGGGACAAAACATGCCAAGCCTAATATTCCTTTGCCACGTTTGCCAAACAGTAGCCGTTCAAATCGAAGCCGGGATAGTTCAAAGAAGATCAGGGATAAGTCTCCGGCGTCAGTCACTAGTTGTCGTTCTGCAAAAGTAGGAGTTGATCCCTTGTTTCATGTAGGATACAGTGAATTGAAGTTCTTTTCTGACACTGACTCAGAGTTTCTGTTTTCTGATGGGGATGATGGTAGCGGTTTAACCAGTGTAACTCATGAGCCTAAGATGGAAGGTTTAGTACGTGCTTCACGATATCGGCCTAAAGCACCACCAAATGGTTTGAGGTCAGGAAGGCCTAGCAAGCGTCCCCCCAAACCCAAGCGGTCACTTTCAGAACCATGGCCTGTTTTTAGGAAAGCCTATGATGTAAAATCCTTGGGTTCTTATAACGAATTTGAGACTGGCCTCAGTGAGCTTAACTGTCAACAATCCAATAGGAAGTCCAACCCTTCTGCATTACCTGAGCTCATTTCACTAGATGACATCCTTTCGTCACCCAATGTTCTAGAAAAGGATATTAATGGGACAGGGGACTTTGGATATGCATCTATCAATAAGAATGTTGAACTTCTGAAGTCTGTATATGCAACAGACGGAGTACCTGTTAAAAACGATCCAGCTCTTATAAAACGAAACCATAGTAAGGTAGATGCTGTGTCTGAGTCAGAGGTAGGTGTTAGGGAAAGAGATTCATCTGGTACTACTACAGAAGCTCCTCTTATGAAACCAAACCCTGGTAATGTAAATGCTGTGTCCAAGTCACAGGGAAGTGTTCGAGAAAGAGATTCATCTGGTGTTACTACTGAAGCTTTTATAAAACCAGACCATAATAAGGTAAATGCTGTGTCACAGTCAGAGGGAAATGTTAAGGAAAGAGCTTCATCCAGTATTACTACAGAAACTCTTATACAACCAGAACATGGTAAGGTAAATCCTCTGTCTCAGTCAGACAGAAATGTTAAGGAAAGAGATTCTTCTGGTGTTTCTAAAGAACAGCCAGCAGTGAAGGAACCCGATAGAGTTGATGAGGAAATGGAAGTGCTAGCTTCACAGGACTCTTCTGCTCGGGAGACTAACTTGTCAGCAAAGAATACAAGTTCAACAGTAGATGGTTGTGTGGGTGAAAAACAGACAACTGATGCCTCCAGCTCTAATGCAAAGCCGATGCTTCACACGTCAGCATCAGTAGAATCTGGTCTTGAATCGCTGGATGGAGGCTATGTCAGTGAAATTGAAGGTGAAAGTATAGTTGATCAGTTAAAACGACAGATTGATTATGACAGGAAATGCATAAAGGAGTTGTATAAGGAATTGGAAGAAGAAAGGAGTGCCGCTGCAATTGCTGCAAATCAGGCAATGGCCATGATCACAAAGCTCCAAGAAGAGAAAGCTGCATTCCATATGGAGGCCCTTCACTGCTTAAGAATGATGGAAGAGCAGGCTGAGTTTGACGTAGAGGCACTGGAGAAAGCCAATGATCTGCTGGCTGAGAAGGAGAAAGAGATACAGGACTTGGAAGCAGAGCTAGAATTTTCTAAGTTAGACTACCAAGATGAATCGATAATGGAGAATCCACTTGACAAAAGTTGTGATTTGATGGGGGGGATTGACCCGGTGGAGAATACTGTTATGCCCGGTGTAAAAAACGATCTCAATGTTACTTGCAACTCAATGGCTACTGAGGTATCCATAGGCAGTAACCAACCTCCTTTTTCTGAAACTACTCTTTTAGAGTTTGAAGATGAAAAATTGTACATTACAAATTGTTTGAAGAGCTTGGAGAGGAAGCTTTCTGAACTTTCCAGTAGAGGGGCTTCCTCCAAAATGCTTAATGGTGGGCATTCCCAAAAGCATATGGATGATGGACAGAATCAAGCAGAGACTCCTAGAAAAGAGAGGAGTCTACTAAATGATGGACAAAATCAAGAAGAGACTCCTAGGAAAGGGAGGAGTCTATTAAATGGTCAGCTTGAAGAGAATGTTCTGCATGGGCAAAAAGATTTACATATGTCTAATGGTAGCTCTGCTGCTCAAGAAGGGCCAACTGGTTGTGGTGGCGGTGATGAATTAGAATGTGAAGACAATAATGCTGATTCCCGTGGGGAAAAAGATATCATGGATCCTGGAGAAATGGATTTGGTTATTCTGGAAAATGAAATCTCAGACCTTAATGATAGGTTGGAGGCACTTGAGACTGATCATGATTTTCTTGAGCACATGCTCTACTCTCTTCAAAATGGAGCGGAAGGACTACAATTTGTTCGAGAGATAGCTCATCAGCTGCAAGAGTTGCGGAAGATAGGGACGGGTTTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

969

Amino Acids

106.95

Weight (kDa)

5.21

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 616 - 706 2.8e-28 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 978, 1217, 1406
AasI GACNNNNNNGTC 1 cut(s) 1669
AccB7I CCANNNNNTGG 3 cut(s) 866, 1325, 1511
AccBSI CCGCTC 1 cut(s) 2834
AccI GTMKAC 1 cut(s) 2494
AciI CCGC 7 cut(s) 146, 786, 913, 1923, 2649, 2834, 2884
AclWI GGATC 5 cut(s) 240, 692, 1199, 2705, 2718
AcoI YGGCCR 1 cut(s) 1950
AcsI RAATTY 3 cut(s) 285, 983, 2120
AcuI CTGAAG 2 cut(s) 1184, 1416
AcyI GRCGYC 1 cut(s) 659
AfaI GTAC 5 cut(s) 27, 831, 1191, 1291, 2345
AfiI CCNNNNNNNGG 8 cut(s) 866, 1234, 1325, 1423, 1511, 1996, 2400, 2534
AflII CTTAAG 1 cut(s) 2011
AflIII ACRYGT 1 cut(s) 1764
AhlI ACTAGT 1 cut(s) 668
AjiI CACGTC 1 cut(s) 1767
AjnI CCWGG 2 cut(s) 1324, 2713
Alw21I GWGCWC 3 cut(s) 185, 1059, 2808
Alw26I GTCTC 7 cut(s) 657, 983, 1536, 1655, 2465, 2516, 2777
AlwI GGATC 5 cut(s) 240, 692, 1199, 2705, 2718
Ama87I CYCGRG 1 cut(s) 1656
AoxI GGCC 7 cut(s) 848, 884, 932, 994, 1950, 1998, 2630
ApeKI GCWGC 8 cut(s) 5, 74, 243, 1925, 1934, 1982, 2618, 2872
ApoI RAATTY 3 cut(s) 285, 983, 2120
ArsI GACNNNNNNTTYG 1 cut(s) 34
AseI ATTAAT 1 cut(s) 1110
AspA2I CCTAGG 1 cut(s) 2528
AspS9I GGNCC 2 cut(s) 1999, 2630
AsuC2I CCSGG 3 cut(s) 623, 2204, 2226
AsuHPI GGTGA 5 cut(s) 331, 1077, 1724, 1841, 2663
AsuNHI GCTAGC 1 cut(s) 1633
AvaI CYCGRG 1 cut(s) 1656
AvrII CCTAGG 1 cut(s) 2528
BaeI ACNNNNGTAYC 2 cut(s) 2259, 2292
BalI TGGCCA 1 cut(s) 1952
BamHI GGATCC 1 cut(s) 2710
BanII GRGCYC 1 cut(s) 1059
BarI GAAGNNNNNNTAC 4 cut(s) 813, 845, 2389, 2421
BbsI GAAGAC 1 cut(s) 2676
Bbv12I GWGCWC 3 cut(s) 185, 1059, 2808
BbvI GCAGC 8 cut(s) 17, 86, 255, 1912, 1921, 1969, 2605, 2859
BceAI ACGGC 1 cut(s) 591
BciT130I CCWGG 2 cut(s) 1326, 2715
BciVI GTATCC 2 cut(s) 710, 2287
BclI TGATCA 3 cut(s) 1843, 1956, 2788
BcnI CCSGG 3 cut(s) 623, 2204, 2226
BcoDI GTCTC 7 cut(s) 657, 983, 1536, 1655, 2465, 2516, 2777
BcuI ACTAGT 1 cut(s) 668
BfaI CTAG 9 cut(s) 279, 669, 888, 1067, 1097, 1634, 2117, 2478, 2529
BfmI CTRYAG 4 cut(s) 72, 499, 1296, 1485
BfrI CTTAAG 1 cut(s) 2011
BfuI GTATCC 2 cut(s) 710, 2287
BisI GCNGC 9 cut(s) 6, 75, 244, 1923, 1926, 1935, 1983, 2619, 2873
BlnI CCTAGG 1 cut(s) 2528
BlsI GCNGC 9 cut(s) 7, 76, 245, 1924, 1927, 1936, 1984, 2620, 2874
Bme1390I CCNGG 5 cut(s) 623, 1326, 2204, 2226, 2715
BmeT110I CYCGRG 1 cut(s) 1656
BmgBI CACGTC 1 cut(s) 1767
BmgT120I GGNCC 2 cut(s) 1999, 2630
BmiI GGNNCC 2 cut(s) 1602, 2712
BmrFI CCNGG 5 cut(s) 623, 1326, 2204, 2226, 2715
BmsI GCATC 7 cut(s) 103, 183, 1145, 1231, 1720, 1746, 1781
BmtI GCTAGC 1 cut(s) 1637
BpiI GAAGAC 1 cut(s) 2676
BpmI CTGGAG 3 cut(s) 1720, 2075, 2736
Bpu10I CCTNAGC 1 cut(s) 1053
BpuEI CTTGAG 4 cut(s) 169, 2607, 2801, 2822
BpuMI CCSGG 3 cut(s) 623, 2204, 2226
Bsa29I ATCGAT 1 cut(s) 2150
BsaAI YACGTR 1 cut(s) 833
BsaBI GATNNNNATC 1 cut(s) 1135
BsaHI GRCGYC 1 cut(s) 659
BsaJI CCNNGG 5 cut(s) 929, 966, 1324, 2528, 2689
BsaXI ACNNNNNCTCC 6 cut(s) 1179, 1209, 1288, 1318, 1797, 1827
Bsc4I CCNNNNNNNGG 8 cut(s) 866, 1234, 1325, 1423, 1511, 1996, 2400, 2534
Bse1I ACTGG 7 cut(s) 208, 795, 997, 1476, 2058, 2394, 2641
Bse3DI GCAATG 3 cut(s) 313, 491, 1953
Bse8I GATNNNNATC 1 cut(s) 1135
BseBI CCWGG 2 cut(s) 1326, 2715
BseCI ATCGAT 1 cut(s) 2150
BseDI CCNNGG 5 cut(s) 929, 966, 1324, 2528, 2689
BseGI GGATG 6 cut(s) 304, 781, 1074, 1472, 1807, 2455
BseJI GATNNNNATC 1 cut(s) 1135
BseLI CCNNNNNNNGG 8 cut(s) 866, 1234, 1325, 1423, 1511, 1996, 2400, 2534
BseMI GCAATG 3 cut(s) 313, 491, 1953
BseMII CTCAG 9 cut(s) 765, 1012, 1044, 1242, 1547, 2025, 2070, 2262, 2766
BseNI ACTGG 7 cut(s) 208, 795, 997, 1476, 2058, 2394, 2641
BseRI GAGGAG 4 cut(s) 439, 1296, 2503, 2554
BseXI GCAGC 8 cut(s) 17, 86, 255, 1912, 1921, 1969, 2605, 2859
BsgI GTGCAG 1 cut(s) 53
BshFI GGCC 7 cut(s) 850, 886, 934, 996, 1952, 2000, 2632
BshVI ATCGAT 1 cut(s) 2150
BsiHKAI GWGCWC 3 cut(s) 185, 1059, 2808
BsiHKCI CYCGRG 1 cut(s) 1656
BsiSI CCGG 4 cut(s) 622, 656, 2204, 2226
BslFI GGGAC 6 cut(s) 362, 525, 568, 882, 1129, 1136
BslI CCNNNNNNNGG 8 cut(s) 866, 1234, 1325, 1423, 1511, 1996, 2400, 2534
BsmAI GTCTC 7 cut(s) 657, 983, 1536, 1655, 2465, 2516, 2777
BsmFI GGGAC 6 cut(s) 362, 525, 568, 882, 1129, 1136
BsmI GAATGC 3 cut(s) 318, 1985, 2431
BsnI GGCC 7 cut(s) 850, 886, 934, 996, 1952, 2000, 2632
BsoBI CYCGRG 1 cut(s) 1656
Bsp1286I GDGCHC 3 cut(s) 185, 1059, 2808
Bsp1407I TGTACA 1 cut(s) 2343
Bsp19I CCATGG 1 cut(s) 929
BspACI CCGC 7 cut(s) 146, 786, 913, 1923, 2649, 2834, 2884
BspANI GGCC 7 cut(s) 850, 886, 934, 996, 1952, 2000, 2632
BspCNI CTCAG 9 cut(s) 764, 1011, 1045, 1243, 1546, 2026, 2071, 2263, 2765
BspDI ATCGAT 1 cut(s) 2150
BspHI TCATGA 2 cut(s) 805, 2791
BspLI GGNNCC 2 cut(s) 1602, 2712
BspMAI CTGCAG 1 cut(s) 76
BspOI GCTAGC 1 cut(s) 1637
BspPI GGATC 5 cut(s) 240, 692, 1199, 2705, 2718
BspQI GCTCTTC 2 cut(s) 2019, 2357
BspTI CTTAAG 1 cut(s) 2011
BsrBI CCGCTC 1 cut(s) 2834
BsrDI GCAATG 3 cut(s) 313, 491, 1953
BsrGI TGTACA 1 cut(s) 2343
BsrI ACTGG 7 cut(s) 208, 795, 997, 1476, 2058, 2394, 2641
BssECI CCNNGG 5 cut(s) 929, 966, 1324, 2528, 2689
BssNI GRCGYC 1 cut(s) 659
BssT1I CCWWGG 3 cut(s) 929, 966, 2528
Bst2UI CCWGG 2 cut(s) 1326, 2715
Bst4CI ACNGT 9 cut(s) 120, 455, 467, 602, 722, 1013, 1443, 1696, 2218
Bst6I CTCTTC 7 cut(s) 1654, 1968, 2019, 2357, 2514, 2559, 2826
BstACI GRCGYC 1 cut(s) 659
BstAFI CTTAAG 1 cut(s) 2011
BstAPI GCANNNNNTGC 3 cut(s) 180, 1934, 2881
BstAUI TGTACA 1 cut(s) 2343
BstBAI YACGTR 1 cut(s) 833
BstC8I GCNNGC 5 cut(s) 893, 1590, 1635, 2031, 2076
BstDSI CCRYGG 2 cut(s) 929, 2689
BstENI CCTNNNNNAGG 1 cut(s) 2532
BstF5I GGATG 6 cut(s) 304, 781, 1074, 1472, 1807, 2455
BstMAI GTCTC 7 cut(s) 657, 983, 1536, 1655, 2465, 2516, 2777
BstMWI GCNNNNNNNGC 7 cut(s) 180, 592, 892, 1931, 1934, 1943, 2881
BstNI CCWGG 2 cut(s) 1326, 2715
BstNSI RCATGY 3 cut(s) 81, 566, 2812
BstSCI CCNGG 5 cut(s) 621, 1324, 2202, 2224, 2713
BstSFI CTRYAG 4 cut(s) 72, 499, 1296, 1485
BstV1I GCAGC 8 cut(s) 17, 86, 255, 1912, 1921, 1969, 2605, 2859
BstV2I GAAGAC 1 cut(s) 2676
BstX2I RGATCY 2 cut(s) 232, 2710
BstYI RGATCY 2 cut(s) 232, 2710
Bsu15I ATCGAT 1 cut(s) 2150
BsuI GTATCC 2 cut(s) 710, 2287
BsuRI GGCC 7 cut(s) 850, 886, 934, 996, 1952, 2000, 2632
BsuTUI ATCGAT 1 cut(s) 2150
BtgI CCRYGG 2 cut(s) 929, 2689
BtrI CACGTC 1 cut(s) 1767
BtsCI GGATG 6 cut(s) 304, 781, 1074, 1472, 1807, 2455
BtsI GCAGTG 2 cut(s) 1599, 2005
BtsIMutI CAGTG 9 cut(s) 116, 727, 744, 802, 1006, 1599, 1822, 2005, 2051
Cac8I GCNNGC 5 cut(s) 893, 1590, 1635, 2031, 2076
CciI TCATGA 2 cut(s) 805, 2791
Cfr13I GGNCC 2 cut(s) 1999, 2630
ClaI ATCGAT 1 cut(s) 2150
CseI GACGC 2 cut(s) 648, 884
Csp6I GTAC 5 cut(s) 26, 830, 1190, 1290, 2344
CviQI GTAC 5 cut(s) 26, 830, 1190, 1290, 2344
DraI TTTAAA 1 cut(s) 529
DrdI GACNNNNNNGTC 1 cut(s) 1669
DseDI GACNNNNNNGTC 1 cut(s) 1669
EaeI YGGCCR 1 cut(s) 1950
Eam1104I CTCTTC 7 cut(s) 1654, 1968, 2019, 2357, 2514, 2559, 2826
EarI CTCTTC 7 cut(s) 1654, 1968, 2019, 2357, 2514, 2559, 2826
Ecl136II GAGCTC 1 cut(s) 1057
Eco130I CCWWGG 3 cut(s) 929, 966, 2528
Eco147I AGGCCT 1 cut(s) 886
Eco24I GRGCYC 1 cut(s) 1059
Eco32I GATATC 2 cut(s) 845, 2704
Eco53kI GAGCTC 1 cut(s) 1057
Eco57I CTGAAG 2 cut(s) 1184, 1416
Eco88I CYCGRG 1 cut(s) 1656
EcoICRI GAGCTC 1 cut(s) 1057
EcoNI CCTNNNNNAGG 1 cut(s) 2532
EcoO109I RGGNCCY 1 cut(s) 1999
EcoRII CCWGG 2 cut(s) 1324, 2713
EcoRV GATATC 2 cut(s) 845, 2704
EcoT14I CCWWGG 3 cut(s) 929, 966, 2528
EcoT22I ATGCAT 4 cut(s) 318, 372, 1138, 1883
EcoT38I GRGCYC 1 cut(s) 1059
ErhI CCWWGG 3 cut(s) 929, 966, 2528
FalI AAGNNNNNCTT 4 cut(s) 1293, 1325, 1549, 1581
FaqI GGGAC 6 cut(s) 362, 525, 568, 882, 1129, 1136
FauNDI CATATG 4 cut(s) 372, 1992, 2445, 2598
FbaI TGATCA 3 cut(s) 1843, 1956, 2788
FblI GTMKAC 1 cut(s) 2494
Fnu4HI GCNGC 9 cut(s) 6, 75, 244, 1923, 1926, 1935, 1983, 2619, 2873
FokI GGATG 6 cut(s) 311, 788, 1061, 1459, 1814, 2462
FriOI GRGCYC 1 cut(s) 1059
Fsp4HI GCNGC 9 cut(s) 6, 75, 244, 1923, 1926, 1935, 1983, 2619, 2873
FspBI CTAG 9 cut(s) 279, 669, 888, 1067, 1097, 1634, 2117, 2478, 2529
GluI GCNGC 9 cut(s) 6, 75, 244, 1923, 1926, 1935, 1983, 2619, 2873
GsuI CTGGAG 3 cut(s) 1720, 2075, 2736
HaeIII GGCC 7 cut(s) 850, 886, 934, 996, 1952, 2000, 2632
HapII CCGG 4 cut(s) 622, 656, 2204, 2226
HgaI GACGC 2 cut(s) 648, 884
Hin1I GRCGYC 1 cut(s) 659
HincII GTYRAC 1 cut(s) 1016
HindII GTYRAC 1 cut(s) 1016
HindIII AAGCTT 2 cut(s) 1398, 2378
HpaII CCGG 4 cut(s) 622, 656, 2204, 2226
HphI GGTGA 5 cut(s) 331, 1077, 1724, 1841, 2663
Hpy166II GTNNAC 4 cut(s) 451, 463, 1016, 2495
Hpy8I GTNNAC 4 cut(s) 451, 463, 1016, 2495
HpyAV CCTTC 9 cut(s) 815, 876, 1050, 1591, 1820, 2012, 2077, 2621, 2831
HpyCH4III ACNGT 9 cut(s) 120, 455, 467, 602, 722, 1013, 1443, 1696, 2218
HpyCH4IV ACGT 4 cut(s) 589, 832, 1766, 2043
HpyF10VI GCNNNNNNNGC 7 cut(s) 180, 592, 892, 1931, 1934, 1943, 2881
HpySE526I ACGT 4 cut(s) 589, 832, 1766, 2043
Hsp92I GRCGYC 1 cut(s) 659
Ksp22I TGATCA 3 cut(s) 1843, 1956, 2788
LguI GCTCTTC 2 cut(s) 2019, 2357
LmnI GCTCC 3 cut(s) 1309, 1971, 2831
Lsp1109I GCAGC 8 cut(s) 17, 86, 255, 1912, 1921, 1969, 2605, 2859
LweI GCATC 7 cut(s) 103, 183, 1145, 1231, 1720, 1746, 1781
MaeI CTAG 9 cut(s) 279, 669, 888, 1067, 1097, 1634, 2117, 2478, 2529
MaeII ACGT 4 cut(s) 589, 832, 1766, 2043
MaeIII GTNAC 9 cut(s) 664, 799, 915, 1083, 1350, 1387, 1437, 2248, 2288
MbiI CCGCTC 1 cut(s) 2834
MfeI CAATTG 1 cut(s) 1929
MflI RGATCY 2 cut(s) 232, 2710
MhlI GDGCHC 3 cut(s) 185, 1059, 2808
MlsI TGGCCA 1 cut(s) 1952
MluNI TGGCCA 1 cut(s) 1952
MlyI GAGTC 7 cut(s) 743, 1262, 1640, 2467, 2500, 2518, 2551
MmeI TCCRAC 3 cut(s) 381, 1059, 2751
Mox20I TGGCCA 1 cut(s) 1952
Mph1103I ATGCAT 4 cut(s) 318, 372, 1138, 1883
MscI TGGCCA 1 cut(s) 1952
MslI CAYNNNNRTG 2 cut(s) 82, 2448
Msp20I TGGCCA 1 cut(s) 1952
MspA1I CMGCKG 2 cut(s) 1925, 2872
MspCI CTTAAG 1 cut(s) 2011
MspI CCGG 4 cut(s) 622, 656, 2204, 2226
MspR9I CCNGG 5 cut(s) 623, 1326, 2204, 2226, 2715
MunI CAATTG 1 cut(s) 1929
Mva1269I GAATGC 3 cut(s) 318, 1985, 2431
MvaI CCWGG 2 cut(s) 1326, 2715
MwoI GCNNNNNNNGC 7 cut(s) 180, 592, 892, 1931, 1934, 1943, 2881
NciI CCSGG 3 cut(s) 623, 2204, 2226
NcoI CCATGG 1 cut(s) 929
NdeI CATATG 4 cut(s) 372, 1992, 2445, 2598
NheI GCTAGC 1 cut(s) 1633
NlaIV GGNNCC 2 cut(s) 1602, 2712
NmuCI GTSAC 5 cut(s) 664, 915, 1083, 1350, 1437
NsiI ATGCAT 4 cut(s) 318, 372, 1138, 1883
NspI RCATGY 3 cut(s) 81, 566, 2812
PagI TCATGA 2 cut(s) 805, 2791
PceI AGGCCT 1 cut(s) 886
PciSI GCTCTTC 2 cut(s) 2019, 2357
PcsI WCGNNNNNNNCGW 1 cut(s) 838
PctI GAATGC 3 cut(s) 318, 1985, 2431
PfeI GAWTC 9 cut(s) 1280, 1376, 1562, 1781, 1793, 2147, 2161, 2461, 2684
PflMI CCANNNNNTGG 3 cut(s) 866, 1325, 1511
PfoI TCCNGGA 1 cut(s) 2713
PkrI GCNGC 9 cut(s) 7, 76, 245, 1924, 1927, 1936, 1984, 2620, 2874
PleI GAGTC 7 cut(s) 743, 1261, 1640, 2467, 2499, 2518, 2550
PpsI GAGTC 7 cut(s) 743, 1261, 1640, 2467, 2499, 2518, 2550
Ppu21I YACGTR 1 cut(s) 833
PshBI ATTAAT 1 cut(s) 1110
PsiI TTATAA 3 cut(s) 978, 1217, 1406
Psp124BI GAGCTC 1 cut(s) 1059
Psp6I CCWGG 2 cut(s) 1324, 2713
PspGI CCWGG 2 cut(s) 1324, 2713
PspN4I GGNNCC 2 cut(s) 1602, 2712
PspPI GGNCC 2 cut(s) 1999, 2630
PsrI GAACNNNNNNTAC 2 cut(s) 199, 231
PstI CTGCAG 1 cut(s) 76
PsuI RGATCY 2 cut(s) 232, 2710
PvuII CAGCTG 1 cut(s) 2872
RsaI GTAC 5 cut(s) 27, 831, 1191, 1291, 2345
RsaNI GTAC 5 cut(s) 26, 830, 1190, 1290, 2344
RseI CAYNNNNRTG 2 cut(s) 82, 2448
SacI GAGCTC 1 cut(s) 1059
SapI GCTCTTC 2 cut(s) 2019, 2357
SatI GCNGC 9 cut(s) 6, 75, 244, 1923, 1926, 1935, 1983, 2619, 2873
Sau96I GGNCC 2 cut(s) 1999, 2630
SchI GAGTC 7 cut(s) 743, 1262, 1640, 2467, 2500, 2518, 2551
ScrFI CCNGG 5 cut(s) 623, 1326, 2204, 2226, 2715
SduI GDGCHC 3 cut(s) 185, 1059, 2808
SfaNI GCATC 7 cut(s) 103, 183, 1145, 1231, 1720, 1746, 1781
SfcI CTRYAG 4 cut(s) 72, 499, 1296, 1485
SmiMI CAYNNNNRTG 2 cut(s) 82, 2448
SmlI CTYRAG 5 cut(s) 184, 2011, 2622, 2780, 2801
SmoI CTYRAG 5 cut(s) 184, 2011, 2622, 2780, 2801
SpeI ACTAGT 1 cut(s) 668
SseBI AGGCCT 1 cut(s) 886
SsiI CCGC 7 cut(s) 146, 786, 913, 1923, 2649, 2834, 2884
SspI AATATT 1 cut(s) 577
SspMI CTAG 9 cut(s) 279, 669, 888, 1067, 1097, 1634, 2117, 2478, 2529
SstI GAGCTC 1 cut(s) 1059
StuI AGGCCT 1 cut(s) 886
StyD4I CCNGG 5 cut(s) 621, 1324, 2202, 2224, 2713
StyI CCWWGG 3 cut(s) 929, 966, 2528
TaaI ACNGT 9 cut(s) 120, 455, 467, 602, 722, 1013, 1443, 1696, 2218
TaiI ACGT 4 cut(s) 592, 835, 1769, 2046
TaqI TCGA 5 cut(s) 257, 616, 1366, 2150, 2854
TatI WGTACW 2 cut(s) 25, 2343
TauI GCSGC 1 cut(s) 1925
TfiI GAWTC 9 cut(s) 1280, 1376, 1562, 1781, 1793, 2147, 2161, 2461, 2684
TscAI CASTG 9 cut(s) 123, 727, 751, 802, 1006, 1599, 1822, 2012, 2058
TseFI GTSAC 5 cut(s) 664, 915, 1083, 1350, 1437
TseI GCWGC 8 cut(s) 5, 74, 243, 1925, 1934, 1982, 2618, 2872
Tsp45I GTSAC 5 cut(s) 664, 915, 1083, 1350, 1437
TspGWI ACGGA 1 cut(s) 1200
TspRI CASTG 9 cut(s) 123, 727, 751, 802, 1006, 1599, 1822, 2012, 2058
Van91I CCANNNNNTGG 3 cut(s) 866, 1325, 1511
Vha464I CTTAAG 1 cut(s) 2011
VspI ATTAAT 1 cut(s) 1110
XagI CCTNNNNNAGG 1 cut(s) 2532
XapI RAATTY 3 cut(s) 285, 983, 2120
XbaI TCTAGA 1 cut(s) 1096
XceI RCATGY 3 cut(s) 81, 566, 2812
XcmI CCANNNNNNNNNTGG 1 cut(s) 2071
XmaJI CCTAGG 1 cut(s) 2528
XmiI GTMKAC 1 cut(s) 2494
XspI CTAG 9 cut(s) 279, 669, 888, 1067, 1097, 1634, 2117, 2478, 2529
Zsp2I ATGCAT 4 cut(s) 318, 372, 1138, 1883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.